##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l05n01_30291.34100000035bb0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12310450 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74528355990236 33.0 33.0 33.0 33.0 33.0 2 32.75970878400059 33.0 33.0 33.0 33.0 33.0 3 32.79648176955351 33.0 33.0 33.0 33.0 33.0 4 36.658284546868714 37.0 37.0 37.0 37.0 37.0 5 36.69889069855286 37.0 37.0 37.0 37.0 37.0 6 36.676366989021524 37.0 37.0 37.0 37.0 37.0 7 36.645600526382054 37.0 37.0 37.0 37.0 37.0 8 36.63752023687193 37.0 37.0 37.0 37.0 37.0 9 36.62634607183328 37.0 37.0 37.0 37.0 37.0 10-11 36.59836805315809 37.0 37.0 37.0 37.0 37.0 12-13 36.57570056334252 37.0 37.0 37.0 37.0 37.0 14-15 38.68305297531772 40.0 37.0 40.0 37.0 40.0 16-17 38.63407487134914 40.0 37.0 40.0 37.0 40.0 18-19 38.58137724453614 40.0 37.0 40.0 37.0 40.0 20-21 38.557001571835315 40.0 37.0 40.0 37.0 40.0 22-23 38.520472200447585 40.0 37.0 40.0 37.0 40.0 24-25 38.47065521569073 40.0 37.0 40.0 37.0 40.0 26-27 38.38790117339334 40.0 37.0 40.0 37.0 40.0 28-29 38.308072491257434 40.0 37.0 40.0 37.0 40.0 30-31 38.26105434813512 40.0 37.0 40.0 37.0 40.0 32-33 38.18700445556418 40.0 37.0 40.0 37.0 40.0 34-35 38.10454991490969 40.0 37.0 40.0 37.0 40.0 36-37 38.00792144072719 40.0 37.0 40.0 37.0 40.0 38-39 37.90338038008359 40.0 37.0 40.0 33.0 40.0 40-41 37.7995611858218 40.0 37.0 40.0 33.0 40.0 42-43 37.66351961138707 40.0 37.0 40.0 33.0 40.0 44-45 37.56171626544928 40.0 37.0 40.0 33.0 40.0 46-47 37.404939665081294 40.0 37.0 40.0 33.0 40.0 48-49 37.23576128411228 40.0 37.0 40.0 33.0 40.0 50-51 37.070098777867585 37.0 37.0 40.0 33.0 40.0 52-53 36.86746467432141 37.0 37.0 40.0 33.0 40.0 54-55 36.65769062057033 37.0 37.0 40.0 33.0 40.0 56-57 36.448990126274836 37.0 37.0 40.0 33.0 40.0 58-59 36.217622101547875 37.0 37.0 40.0 33.0 40.0 60-61 35.94324179863449 37.0 37.0 40.0 33.0 40.0 62-63 35.70238728072491 37.0 37.0 38.5 33.0 40.0 64-65 35.42155099935421 37.0 33.0 37.0 33.0 40.0 66-67 35.136633266858645 37.0 33.0 37.0 30.0 40.0 68-69 34.85151761308482 37.0 33.0 37.0 27.0 40.0 70-71 34.58141278344821 37.0 33.0 37.0 27.0 37.0 72-73 34.35945038564796 37.0 33.0 37.0 27.0 37.0 74-75 34.04552936732614 37.0 33.0 37.0 27.0 37.0 76-77 31.21842056951614 33.0 30.0 35.0 24.5 37.0 78-79 33.73785783622857 35.0 33.0 37.0 27.0 37.0 80-81 34.172321686047226 37.0 33.0 37.0 27.0 37.0 82-83 34.113259547782576 37.0 33.0 37.0 27.0 37.0 84-85 34.03086666206353 37.0 33.0 37.0 27.0 37.0 86-87 33.893414334975574 37.0 33.0 37.0 27.0 37.0 88-89 33.78215507962747 37.0 33.0 37.0 27.0 37.0 90-91 33.701675446470276 37.0 33.0 37.0 27.0 37.0 92-93 33.61136818719056 37.0 33.0 37.0 27.0 37.0 94-95 33.47772481915771 37.0 33.0 37.0 27.0 37.0 96-97 33.36949096093157 37.0 33.0 37.0 27.0 37.0 98-99 33.212814397524056 37.0 33.0 37.0 27.0 37.0 100-101 31.44158682257757 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.08821437906426155 2306 2 0.10391765962258148 2306 3 0.07057135500132716 2306 4 0.07298039175263682 2306 5 0.07277548336609385 2306 6 0.09539629761040658 2306 7 0.11051828947736198 2306 8 0.10068486700119905 2306 9 0.09623959317497821 2306 10-11 0.1124323946112682 2306 12-13 0.06854352920160522 2306 14-15 0.10852343661998987 2306 16-17 0.12596995620082652 2306 18-19 0.1389759960528565 2306 20-21 0.13859815642262419 2306 22-23 0.14365826532436188 2306 24-25 0.12332285462515102 2306 26-27 0.13897943798453838 2306 28-29 0.11448574090093189 2306 30-31 0.12612046504506935 2306 32-33 0.13704582156923806 2306 34-35 0.10230296204893108 2306 36-37 0.13756873006662573 2306 38-39 0.09960679247961224 2306 40-41 0.06795176466454222 2306 42-43 0.08487522541129522 2306 44-45 0.0737850036647032 2306 46-47 0.09020939707587416 2306 48-49 0.06428238690362775 2306 50-51 0.007643942603252185 2306 52-53 -0.03839088479174535 2306 54-55 0.049208424552055874 2306 56-57 0.05872060548016833 2306 58-59 -0.047457978074561424 2306 60-61 -0.01281108119487584 2306 62-63 -0.007130909679958108 2306 64-65 -0.11943209455504444 2306 66-67 -0.18174880727669773 2306 68-69 -0.181920219889129 2306 70-71 -0.21936246850148677 2306 72-73 -0.19138376606448304 2306 74-75 -0.21591334260167372 2306 76-77 -0.49589047347282644 2306 78-79 -0.11413663119115824 2306 80-81 -0.03568273851346504 2306 82-83 -0.12354939066565152 2306 84-85 -0.12584850363075617 2306 86-87 -0.16280763931753484 2306 88-89 -0.051878062988855334 2306 90-91 -0.0754003031635051 2306 92-93 -0.12152864824350473 2306 94-95 -0.16476695748141879 2306 96-97 -0.2854341274601211 2306 98-99 -0.14340289019217067 2306 100-101 -0.2592113472458024 2307 1 0.08183206683149535 2307 2 0.08984407057195654 2307 3 0.05579545529757013 2307 4 0.053075156747802055 2307 5 0.053285104771084946 2307 6 0.07446547580209995 2307 7 0.0392511149939736 2307 8 0.08483186420946254 2307 9 0.058558348274537764 2307 10-11 0.08965587197321412 2307 12-13 0.0985723446901261 2307 14-15 0.1582696896192104 2307 16-17 0.13289699836482072 2307 18-19 0.1051328608461759 2307 20-21 0.09648437091202311 2307 22-23 0.11561611343667266 2307 24-25 0.056925727001960524 2307 26-27 0.09590855022671718 2307 28-29 0.1130865718621692 2307 30-31 0.10576562816404333 2307 32-33 0.07717049123252906 2307 34-35 0.10279762793512504 2307 36-37 0.11668257099162815 2307 38-39 0.05431153244911968 2307 40-41 0.03629542184940249 2307 42-43 0.0627014140024329 2307 44-45 -0.028661140862418222 2307 46-47 -0.0024755261942814855 2307 48-49 -0.07731162426459548 2307 50-51 -0.0731251010015086 2307 52-53 -0.08225596282732539 2307 54-55 -0.052139737759908655 2307 56-57 -0.03297083949174606 2307 58-59 -0.004708468364626128 2307 60-61 -0.13576523565073018 2307 62-63 -0.07284495608410424 2307 64-65 -0.12915930920431862 2307 66-67 -0.27089547008771575 2307 68-69 -0.18483624668931498 2307 70-71 -0.20217492769706524 2307 72-73 -0.3538969811252244 2307 74-75 -0.412494547947702 2307 76-77 -0.6790143807103952 2307 78-79 -0.26163470920317167 2307 80-81 -0.07279364183825976 2307 82-83 -0.22256465128212 2307 84-85 -0.1904138540886251 2307 86-87 -0.12834174815523625 2307 88-89 -0.20978881905147517 2307 90-91 -0.2714168994581243 2307 92-93 -0.19948418805937962 2307 94-95 -0.12771558323611742 2307 96-97 -0.4088428519798484 2307 98-99 -0.23187398139089765 2307 100-101 -0.2909612002702495 2201 1 0.12548468419294068 2201 2 0.11586238784843772 2201 3 0.08884358388909419 2201 4 0.0049873531545046035 2201 5 0.029250397739133405 2201 6 0.05975930621987402 2201 7 0.08678232650036222 2201 8 0.08417071517852293 2201 9 0.06051904735634395 2201 10-11 0.08556830378453384 2201 12-13 0.08203117277133742 2201 14-15 0.1550228422348212 2201 16-17 0.16856853083725554 2201 18-19 0.15815708188412003 2201 20-21 0.1413884027405956 2201 22-23 0.07921450033232702 2201 24-25 0.1204207106693147 2201 26-27 0.11359956884117395 2201 28-29 0.09553991338416523 2201 30-31 0.15763506716686493 2201 32-33 0.10809462335279107 2201 34-35 0.1304331866190509 2201 36-37 0.1435213724324882 2201 38-39 0.12866370941746652 2201 40-41 0.15543088089423662 2201 42-43 0.12384816664228282 2201 44-45 0.11460032608129467 2201 46-47 0.07398888284105709 2201 48-49 0.07269856689094922 2201 50-51 0.05183467044624024 2201 52-53 0.050446686173899025 2201 54-55 0.042722206752188185 2201 56-57 0.08314366154951358 2201 58-59 0.006522511349793092 2201 60-61 -0.009057637325604162 2201 62-63 -0.018419040365948547 2201 64-65 -0.03164584627413802 2201 66-67 -0.09720014193716509 2201 68-69 -0.11715305935734932 2201 70-71 -0.09212414089790144 2201 72-73 -0.060509019158217825 2201 74-75 -0.05374449987620977 2201 76-77 -0.037718775434999685 2201 78-79 -0.009718952592898233 2201 80-81 0.0262149259576816 2201 82-83 -0.012914827311170995 2201 84-85 -0.030610131432560195 2201 86-87 -0.029093335679725385 2201 88-89 -0.011690789341542995 2201 90-91 -0.06388025150340582 2201 92-93 -0.042752553990048625 2201 94-95 -0.03781694706794525 2201 96-97 -0.12515645638587358 2201 98-99 -0.06254045033322342 2201 100-101 -0.09706248019547914 1101 1 0.06750321088588862 1101 2 0.07336251225762425 1101 3 0.03504527384986034 1101 4 -0.011089115778275982 1101 5 0.013902720352440667 1101 6 0.040257890672648955 1101 7 0.038842245360100947 1101 8 0.07048230812729628 1101 9 0.07224433693252053 1101 10-11 0.07516574284058919 1101 12-13 0.06767766488242444 1101 14-15 0.1143337784050189 1101 16-17 0.09799069136535365 1101 18-19 0.1035871164520401 1101 20-21 0.04051503753247232 1101 22-23 0.054677930910898453 1101 24-25 0.08577952543787859 1101 26-27 0.0822423614562382 1101 28-29 0.05507238706843509 1101 30-31 0.017905805328588542 1101 32-33 0.07506332692757667 1101 34-35 0.06656357101031318 1101 36-37 0.03800686530261288 1101 38-39 -0.02155038052978142 1101 40-41 -0.10530463384302635 1101 42-43 0.02648634618180523 1101 44-45 0.031061979434333864 1101 46-47 0.04030685844802662 1101 48-49 -0.023279296936280502 1101 50-51 -0.01473600441541123 1101 52-53 -0.09650045257622253 1101 54-55 -0.11074734883442972 1101 56-57 -0.018314108368734594 1101 58-59 0.011518962503515695 1101 60-61 0.054256828838411764 1101 62-63 -0.14277156062426144 1101 64-65 -0.1986002321776752 1101 66-67 -0.13570790515515085 1101 68-69 -0.13106650691161548 1101 70-71 -0.25324938332597213 1101 72-73 -0.38345986827425804 1101 74-75 -0.2705884970439243 1101 76-77 -0.4909737867473183 1101 78-79 -0.10827193791227785 1101 80-81 -0.008784307206184394 1101 82-83 -0.07406073100504784 1101 84-85 -0.04315662777272422 1101 86-87 -0.03612421667168064 1101 88-89 -0.10372583740424801 1101 90-91 -0.09440981936279513 1101 92-93 -0.1731363083416042 1101 94-95 -0.1615516674546953 1101 96-97 -0.1900549953889552 1101 98-99 -0.17021729947405362 1101 100-101 -0.06852919143998193 2304 1 0.08874595596860502 2304 2 0.0920554254756496 2304 3 0.04525993297579589 2304 4 -0.01791397683626883 2304 5 -0.02949905891022553 2304 6 0.035545997758326564 2304 7 0.0012811012112550202 2304 8 0.03875501132714021 2304 9 0.027117715514989982 2304 10-11 0.05679716245825972 2304 12-13 0.02736477258808634 2304 14-15 0.02061269793487952 2304 16-17 0.0322542740977525 2304 18-19 0.05972272337098872 2304 20-21 0.004361940279181908 2304 22-23 0.04818243431681424 2304 24-25 0.006895453757501002 2304 26-27 0.003930662151077513 2304 28-29 0.0388252200724466 2304 30-31 0.03044154468788207 2304 32-33 -0.009016542179054454 2304 34-35 -0.02589260745424582 2304 36-37 -0.05730537386666157 2304 38-39 0.0035119721964349537 2304 40-41 -0.11468014563854467 2304 42-43 -0.053173138871500214 2304 44-45 0.01742781590207443 2304 46-47 -0.052875956193496165 2304 48-49 -0.02518876038318041 2304 50-51 -0.08831596053961022 2304 52-53 -0.139894595603117 2304 54-55 -0.1468662902114275 2304 56-57 -0.10086950264909689 2304 58-59 -0.11186348741401986 2304 60-61 -0.12619574934816313 2304 62-63 -0.13996894370402657 2304 64-65 -0.21807250514370224 2304 66-67 -0.3026711123110388 2304 68-69 -0.23256139379075336 2304 70-71 -0.3143584193015201 2304 72-73 -0.2930843963371288 2304 74-75 -0.3410541318124416 2304 76-77 -0.48347199410644137 2304 78-79 -0.22527156024418815 2304 80-81 -0.02133191528852052 2304 82-83 -0.02157633003913162 2304 84-85 -0.09504398165325512 2304 86-87 -0.05455935266969192 2304 88-89 0.016380884416953734 2304 90-91 -0.09043366888593596 2304 92-93 -0.22196469864283586 2304 94-95 -0.2520795878610542 2304 96-97 -0.22652513454998058 2304 98-99 -0.15218256269135821 2304 100-101 0.060920425386729704 2202 1 0.06216044317854852 2202 2 0.049932676045443714 2202 3 0.035408087826823476 2202 4 -0.01382853489729996 2202 5 -0.011241113405688452 2202 6 -0.0017647011376951127 2202 7 -0.001981821183072441 2202 8 0.035889146594470844 2202 9 -0.0036987413641327294 2202 10-11 0.014993447929704473 2202 12-13 0.02679868839476285 2202 14-15 0.04137748683373843 2202 16-17 0.07638718945071332 2202 18-19 0.05091683154996929 2202 20-21 0.02394874679632153 2202 22-23 0.08804046424121736 2202 24-25 0.021452544283128816 2202 26-27 0.04328326187545173 2202 28-29 0.003324873380563531 2202 30-31 0.03589708251319479 2202 32-33 0.044140673135601105 2202 34-35 0.06130312587996656 2202 36-37 0.06816710260690684 2202 38-39 0.12515456270880776 2202 40-41 0.029873069264581886 2202 42-43 0.07024076057063411 2202 44-45 0.05096935117530421 2202 46-47 0.09678777682832873 2202 48-49 0.07243720896669004 2202 50-51 0.013424173914330595 2202 52-53 0.059052006009956415 2202 54-55 0.06980047213837537 2202 56-57 0.0390181070322555 2202 58-59 0.03829173796486174 2202 60-61 0.09236162057221264 2202 62-63 0.07311800738576579 2202 64-65 0.0878862074935185 2202 66-67 -0.03279293698654584 2202 68-69 0.011101680931126623 2202 70-71 0.06634133605037817 2202 72-73 -0.09828287459258434 2202 74-75 -0.08151769016243549 2202 76-77 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32-33 -0.017812913989445178 1102 34-35 0.004564972418542368 1102 36-37 -0.00999690769436512 1102 38-39 0.01973429287826889 1102 40-41 -0.01442059206769386 1102 42-43 -0.011088855272220144 1102 44-45 -0.02856681370194991 1102 46-47 -0.03916098054859418 1102 48-49 0.010299159706626426 1102 50-51 -0.00740378189956914 1102 52-53 -0.08064765652350303 1102 54-55 -0.08491763942383557 1102 56-57 -0.1039204962966025 1102 58-59 -0.08274545757670637 1102 60-61 -0.04980252751681036 1102 62-63 -0.017005279794027217 1102 64-65 -0.0788049168633691 1102 66-67 -0.09709655070229672 1102 68-69 -0.11104544302560981 1102 70-71 -0.14470962490447903 1102 72-73 -0.14196596625797042 1102 74-75 -0.16714927393221757 1102 76-77 -0.3996110235984105 1102 78-79 -0.05757149946769857 1102 80-81 0.03725922022718464 1102 82-83 0.03686571495168067 1102 84-85 -0.012422972164394253 1102 86-87 0.007172390305967724 1102 88-89 -0.04351321363736105 1102 90-91 -0.1707008430895698 1102 92-93 -0.21434160731482166 1102 94-95 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-0.3082688221804233 1213 24-25 -0.25890515440509176 1213 26-27 -0.29620901951781775 1213 28-29 -0.2797648732239253 1213 30-31 -0.24732135068359895 1213 32-33 -0.2644571250941752 1213 34-35 -0.24483577581340654 1213 36-37 -0.2175113566958231 1213 38-39 -0.1409116408094775 1213 40-41 -0.17366994261951163 1213 42-43 -0.149468279900276 1213 44-45 -0.15051381144336062 1213 46-47 -0.25592975254162553 1213 48-49 -0.1261017894444194 1213 50-51 -0.08623232807983072 1213 52-53 -0.037479406426847106 1213 54-55 0.04137589242525763 1213 56-57 0.04526607300847019 1213 58-59 0.03844692344061684 1213 60-61 0.10251804791000296 1213 62-63 0.11209995284214358 1213 64-65 0.1867056959086142 1213 66-67 0.1734758999590369 1213 68-69 0.2174915070404353 1213 70-71 0.17469610100631172 1213 72-73 0.18665705068296745 1213 74-75 0.28544836729024325 1213 76-77 0.6832896346571218 1213 78-79 0.24213274354298875 1213 80-81 0.07987934786950035 1213 82-83 0.10174554577969985 1213 84-85 0.11737672404452582 1213 86-87 0.10627467377949529 1213 88-89 0.15161816263930916 1213 90-91 0.14621499311508046 1213 92-93 0.2204063937340024 1213 94-95 0.2894156622654762 1213 96-97 0.25896910434824605 1213 98-99 0.31664295417638044 1213 100-101 0.5965381174226962 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 29.0 8 375.0 9 1070.0 10 755.0 11 298.0 12 291.0 13 514.0 14 1268.0 15 3052.0 16 5893.0 17 9169.0 18 12832.0 19 16344.0 20 20192.0 21 24982.0 22 30011.0 23 35873.0 24 44092.0 25 54097.0 26 66340.0 27 82467.0 28 102407.0 29 131349.0 30 169266.0 31 222742.0 32 297872.0 33 415718.0 34 637525.0 35 1193780.0 36 2760960.0 37 4747510.0 38 1221343.0 39 34.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.91437213444452 25.410674711724017 31.117048881727698 23.557904272103762 2 23.826976268129922 24.207100471550593 32.09699889118595 19.86892436913354 3 21.426877165335142 22.743839583443336 36.308591481221235 19.520691770000283 4 20.91356476878658 22.60488540625606 36.32849829539014 20.15305152956723 5 26.125129463179658 21.95374661364938 32.769679418705245 19.15144450446572 6 26.661218720680395 22.167784280834578 32.39585067970708 18.77514631877795 7 26.799329025340256 25.50161854359508 28.92613998675922 18.772912444305447 8 25.361371842621512 23.942455393588375 31.307572022143788 19.388600741646325 9 22.01570210674671 22.702581952731215 35.360193981536014 19.921521958986066 10-11 25.581767695834746 23.537735226402017 30.660470794995838 20.220026282767403 12-13 25.398555698613777 26.83983932350158 27.10153974875005 20.6600652291346 14-15 25.60343712744903 24.55172847404746 28.048988026055376 21.795846372448132 16-17 24.961577359072983 24.513973900222982 28.21524395940035 22.30920478130369 18-19 24.28892932427328 24.990162829141095 28.86957016193559 21.851337684650034 20-21 23.068992672121 25.91252832865447 29.132762587475813 21.885716411748717 22-23 22.977299773769438 25.76049210223834 29.080618498917588 22.181589625074633 24-25 23.234455279863855 26.012282248008805 28.66220568703825 22.09105678508909 26-27 23.18081386139418 25.909775840850656 28.681128634615305 22.228281663139853 28-29 23.417125287865186 25.894626110337153 28.328606996494848 22.359641605302812 30-31 23.299874480563005 25.751118318721893 28.245207890145963 22.70379931056914 32-33 23.230312458114856 26.026201316767462 27.927788992278916 22.815697232838765 34-35 23.037766791521484 26.063456833336833 27.991833592510545 22.906942782631138 36-37 23.21581722679621 26.25423360653807 28.111488586991225 22.418460579674495 38-39 23.415806037801854 26.230559916882118 27.876620898023596 22.477013147292432 40-41 23.099485444904243 26.730251321157724 27.67848475023577 22.491778483702262 42-43 23.211612867588226 26.752002995862817 27.56577616648159 22.470607970067366 44-45 23.064493829310656 26.26909641638128 28.28909691352619 22.37731284078187 46-47 22.952795313405755 26.450921579025355 28.098213218072594 22.4980698894963 48-49 23.01407898441977 26.272788532628056 28.0154312918372 22.697701191114973 50-51 22.999384094696374 26.307125379376384 28.183252379667362 22.51023814625988 52-53 23.299913113549234 26.818773745699755 27.47910752161721 22.402205619133802 54-55 23.10601875172649 26.431245023642774 27.79282104613185 22.669915178498886 56-57 23.16719759875022 26.759322321714578 27.564631493592557 22.508848585942644 58-59 23.104875577319888 26.637874590025984 27.75882577281611 22.498424059838023 60-61 23.100311088014294 26.521686162764013 27.575930908977313 22.802071840244377 62-63 22.81134145550921 26.839707116865846 27.53923870726032 22.809712720364615 64-65 23.239160010648654 26.760722194506588 27.36084630965405 22.639271485190708 66-67 23.44950010409209 26.76980821667403 27.076470618103897 22.704221061129985 68-69 23.32474267117387 26.947089118420113 27.02171757583661 22.706450634569403 70-71 23.35834648361133 27.193006543509007 26.936653301911317 22.511993670968344 72-73 23.40353239067459 27.0043495764373 26.782873385361423 22.80924464752669 74-75 23.419139219527356 26.976353468174924 26.893592130076744 22.710915182220976 76-77 23.3368567727367 26.71780264782429 27.168671265216187 22.77666931422283 78-79 23.613100066279173 26.637265582155734 26.929089733113504 22.820544618451592 80-81 23.59484987745347 26.688266322003702 26.886217353758568 22.83066644678426 82-83 23.399229396356418 26.808640577042514 27.081117440676017 22.711012585925054 84-85 23.04059801803544 27.327191523555022 26.843899775674174 22.78831068273536 86-87 22.91029114171214 27.04576843463759 26.986463587372146 23.057476836278123 88-89 22.635248654549024 27.619173273547787 26.81242263717816 22.933155434725034 90-91 22.704636037690346 27.458213774079383 26.846973839558203 22.99017634867207 92-93 22.66214271281779 27.863556050217415 26.512927645870434 22.961373591094365 94-95 22.68298265809719 28.040752492467362 26.357412226641557 22.91885262279389 96-97 22.517118661516612 28.110852012194833 26.37333417403479 22.99869515225377 98-99 22.906366383825976 27.906351147848103 26.1740326525289 23.013249815797028 100-101 23.220693350713862 28.06722186806734 25.81810287499801 22.89398190622079 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 34972.0 1 18923.0 2 3800.0 3 4650.5 4 4976.0 5 3672.0 6 1612.5 7 1359.0 8 1578.5 9 1757.5 10 1727.5 11 1446.5 12 1043.5 13 723.0 14 601.5 15 596.5 16 647.0 17 678.5 18 753.0 19 901.0 20 1081.5 21 1313.0 22 1646.0 23 2056.5 24 2634.5 25 3270.5 26 3774.5 27 4488.0 28 5642.0 29 7177.0 30 9742.0 31 14088.0 32 31392.5 33 59276.5 34 91267.5 35 161919.0 36 235997.0 37 339090.0 38 475649.5 39 516675.5 40 512900.0 41 527818.0 42 578105.5 43 682637.0 44 771475.5 45 810781.0 46 792685.0 47 709568.0 48 640613.5 49 615487.0 50 559303.0 51 490506.5 52 444916.5 53 390488.0 54 318985.0 55 267928.5 56 236126.0 57 191451.0 58 150285.0 59 120183.0 60 93020.5 61 76931.0 62 65171.0 63 48719.5 64 33706.0 65 25431.0 66 20925.5 67 16685.0 68 13213.0 69 10414.0 70 8107.5 71 6853.5 72 7764.0 73 7527.0 74 5107.5 75 3536.0 76 2809.5 77 2550.0 78 2643.5 79 2436.5 80 1774.5 81 1164.5 82 807.0 83 620.0 84 499.0 85 379.5 86 295.5 87 294.5 88 308.0 89 276.0 90 233.0 91 193.0 92 168.5 93 138.5 94 100.0 95 85.0 96 79.5 97 66.0 98 38.5 99 13.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13827276825786222 2 0.0 3 0.0 4 0.18115503494998153 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.326259397503748E-4 12-13 0.0 14-15 4.386517146001974E-4 16-17 0.0 18-19 0.0 20-21 0.0038666336324017403 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 4.873907940002193E-5 32-33 0.0 34-35 1.5027882815006762E-4 36-37 1.9495631760008773E-4 38-39 0.009630029771454333 40-41 0.007968839481903587 42-43 0.0011372451860005117 44-45 0.0012672160644005702 46-47 0.0022257512926010014 48-49 7.717020905003472E-5 50-51 9.422888684004239E-4 52-53 0.01975557351680889 54-55 0.018277154775008225 56-57 0.021871661880759844 58-59 0.04140384795031863 60-61 0.015235023902456854 62-63 0.002306983091601038 64-65 0.007416463248703337 66-67 0.013301707086255986 68-69 0.02828897400176273 70-71 0.01184359629420533 72-73 0.019130088664508608 74-75 0.012692468593755713 76-77 0.023341957442660502 78-79 0.009370088014654217 80-81 0.006823471116003071 82-83 0.0018723929669508426 84-85 0.019983022554008995 86-87 0.009500058893054275 88-89 0.012720899723405723 90-91 0.04636304927927087 92-93 0.03574605314996608 94-95 0.029803947053113414 96-97 0.026538428733311945 98-99 0.03291918654476481 100-101 0.2440325089659598 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.231045E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.476140704263456 #Duplication Level Percentage of deduplicated Percentage of total 1 66.95237305018253 10.361643458089615 2 12.45590278432897 3.8553860817780423 3 5.655078379792669 2.6255636609792865 4 2.7299986517009454 1.6899937302469341 5 1.9611225571955777 1.517530431673186 6 1.4374165076220249 1.3347396073553663 7 0.9408895202924473 1.0192937022249002 8 0.9636217886872309 1.193051710993409 9 0.5932357132744196 0.8262899432486108 >10 4.901807516046815 15.60118571797825 >50 0.7778448422131682 8.337092797238 >100 0.44733919802138605 13.501862562433455 >500 0.08405181544594363 9.425697380046415 >1k 0.09767117138449384 26.697552825057386 >5k 0.0013302517024100726 1.268044813084755 >10k+ 3.162521089183739E-4 0.7450715775722636 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21921 0.17806822658798013 No Hit CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT 18743 0.1522527608657685 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14959 0.12151464812415469 No Hit CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC 13932 0.11317214236685094 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.747815880004387E-5 0.0 0.0 2 9.747815880004387E-5 0.0 0.0 3 1.0560133870004752E-4 0.0 0.0 4 1.0560133870004752E-4 0.0 0.0 5 1.0560133870004752E-4 0.0 0.0 6 1.0560133870004752E-4 0.0 0.0 7 1.2184769850005484E-4 0.0 0.0 8 1.2997087840005848E-4 0.0 0.0 9 1.9495631760008773E-4 0.0 0.0 10-11 2.924344764001316E-4 0.0 0.0 12-13 4.3052853470019374E-4 0.0 0.0 14-15 6.864087015503088E-4 0.0 0.0 16-17 0.0010560133870004752 0.0 0.0 18-19 0.0016002664403007203 0.0 0.0 20-21 0.0022257512926010014 0.0 0.0 22-23 0.002899975224301305 0.0 0.0 24-25 0.0036676157248516504 0.0 0.0 26-27 0.004544919154052045 0.0 0.0 28-29 0.0054181609933024386 0.0 0.0 30-31 0.006551344589352948 0.0 0.0 32-33 0.007798252704003509 0.0 0.0 34-35 0.00906546876840408 0.0 0.0 36-37 0.010474840481054713 0.0 0.0 38-39 0.01195732081280538 0.0 0.0 40-41 0.013817529009906218 0.0 0.0 42-43 0.015925494193957168 0.0 0.0 44-45 0.01828933954485823 0.0 0.0 46-47 0.021022789581209463 0.0 0.0 48-49 0.024442648319111 0.0 0.0 50-51 0.028764180025912946 0.0 0.0 52-53 0.03392646085236527 0.0 0.0 54-55 0.04012038552611805 0.0 0.0 56-57 0.04764245011352144 0.0 0.0 58-59 0.0571100162869757 0.0 0.0 60-61 0.0695466047138813 0.0 0.0 62-63 0.08531369689978839 0.0 0.0 64-65 0.10449658623364702 0.0 4.0615899500018274E-6 66-67 0.12941037898695823 0.0 8.123179900003655E-6 68-69 0.1606399441125223 8.123179900003655E-6 8.123179900003655E-6 70-71 0.2004232176727902 8.123179900003655E-6 8.123179900003655E-6 72-73 0.2504092051874627 8.123179900003655E-6 8.123179900003655E-6 74-75 0.3137375156878912 8.123179900003655E-6 8.123179900003655E-6 76-77 0.3907980618092759 8.123179900003655E-6 8.123179900003655E-6 78-79 0.48641195082226885 8.123179900003655E-6 8.123179900003655E-6 80-81 0.6027927492496212 8.123179900003655E-6 8.123179900003655E-6 82-83 0.7435227794272345 8.123179900003655E-6 8.123179900003655E-6 84-85 0.9123752584186606 8.123179900003655E-6 8.123179900003655E-6 86-87 1.1139397828674014 8.123179900003655E-6 8.123179900003655E-6 88-89 1.3493739059092071 8.123179900003655E-6 8.123179900003655E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTTGT 44005 0.0 58.903038 1 CTTTGTG 111325 0.0 50.935184 1 TTTGTGT 123115 0.0 46.162464 2 TTGTGTT 127905 0.0 45.69441 3 TGTGTTT 136215 0.0 43.04294 4 GTGTTTG 151865 0.0 38.62144 5 AACTTTG 24065 0.0 32.989456 2 TAACTTT 24330 0.0 32.5756 1 TTGAGGG 36945 0.0 31.949142 9 TTTGAGG 54635 0.0 31.781013 8 GTTTGAG 118065 0.0 30.83718 7 TGTTTGA 197910 0.0 29.645517 6 GTTTGAC 33595 0.0 27.276987 7 TTGACGG 8025 0.0 25.855333 9 TTTGACG 11855 0.0 25.832825 8 TTTGACC 10120 0.0 25.006933 8 TTGAGTC 15415 0.0 24.932884 4 TTGACCC 7010 0.0 24.315887 9 TTGAGGC 7710 0.0 23.709372 9 GTTTGAT 48460 0.0 22.40766 7 >>END_MODULE