##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l05n01_30191.34100000035b8a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16297633 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.755006938737665 33.0 33.0 33.0 33.0 33.0 2 32.77134765520858 33.0 33.0 33.0 33.0 33.0 3 32.80549058872537 33.0 33.0 33.0 33.0 33.0 4 36.675957790925835 37.0 37.0 37.0 37.0 37.0 5 36.718321672846606 37.0 37.0 37.0 37.0 37.0 6 36.691299589333006 37.0 37.0 37.0 37.0 37.0 7 36.66703735444282 37.0 37.0 37.0 37.0 37.0 8 36.66254357304524 37.0 37.0 37.0 37.0 37.0 9 36.65452161059216 37.0 37.0 37.0 37.0 37.0 10-11 36.63016003612304 37.0 37.0 37.0 37.0 37.0 12-13 36.61552956800537 37.0 37.0 37.0 37.0 37.0 14-15 38.78540362272239 40.0 37.0 40.0 37.0 40.0 16-17 38.74666720007746 40.0 37.0 40.0 37.0 40.0 18-19 38.71710717132973 40.0 37.0 40.0 37.0 40.0 20-21 38.68063356194118 40.0 37.0 40.0 37.0 40.0 22-23 38.640489634292294 40.0 37.0 40.0 37.0 40.0 24-25 38.599066502479225 40.0 37.0 40.0 37.0 40.0 26-27 38.53492807820621 40.0 37.0 40.0 37.0 40.0 28-29 38.466904703278075 40.0 37.0 40.0 37.0 40.0 30-31 38.42672696703871 40.0 37.0 40.0 37.0 40.0 32-33 38.36571080598023 40.0 37.0 40.0 37.0 40.0 34-35 38.2927542606954 40.0 37.0 40.0 37.0 40.0 36-37 38.20720337118893 40.0 37.0 40.0 37.0 40.0 38-39 38.1122437841127 40.0 37.0 40.0 37.0 40.0 40-41 38.02367822370279 40.0 37.0 40.0 37.0 40.0 42-43 37.89662575540878 40.0 37.0 40.0 33.0 40.0 44-45 37.78659683280388 40.0 37.0 40.0 33.0 40.0 46-47 37.606995230534395 40.0 37.0 40.0 33.0 40.0 48-49 37.42173400272297 38.5 37.0 40.0 33.0 40.0 50-51 37.242614771114305 37.0 37.0 40.0 33.0 40.0 52-53 37.01997934301257 37.0 37.0 40.0 33.0 40.0 54-55 36.77274871755917 37.0 37.0 40.0 33.0 40.0 56-57 36.51358347558815 37.0 37.0 40.0 33.0 40.0 58-59 36.244170917335055 37.0 37.0 40.0 33.0 40.0 60-61 35.975588448948386 37.0 37.0 38.5 33.0 40.0 62-63 35.72206899615423 37.0 35.0 37.0 33.0 40.0 64-65 35.441294603946474 37.0 33.0 37.0 33.0 40.0 66-67 35.16225939067348 37.0 33.0 37.0 33.0 40.0 68-69 34.88335953447964 37.0 33.0 37.0 33.0 37.0 70-71 34.65984670902824 37.0 33.0 37.0 33.0 37.0 72-73 34.48815404666432 37.0 33.0 37.0 27.0 37.0 74-75 34.23780085120336 37.0 33.0 37.0 27.0 37.0 76-77 31.323342690315826 33.0 30.0 35.0 24.5 37.0 78-79 33.98063123645011 35.0 33.0 37.0 30.0 37.0 80-81 34.46374071621321 37.0 33.0 37.0 33.0 37.0 82-83 34.439721522751185 37.0 33.0 37.0 33.0 37.0 84-85 34.36831400608911 37.0 33.0 37.0 33.0 37.0 86-87 34.2342033042467 37.0 33.0 37.0 27.0 37.0 88-89 34.13124120539467 37.0 33.0 37.0 27.0 37.0 90-91 34.04823369135874 37.0 33.0 37.0 27.0 37.0 92-93 33.97231168477042 37.0 33.0 37.0 27.0 37.0 94-95 33.85059851942917 37.0 33.0 37.0 27.0 37.0 96-97 33.76113288966563 37.0 33.0 37.0 27.0 37.0 98-99 33.60830901027161 37.0 33.0 37.0 27.0 37.0 100-101 31.75316661014517 35.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.08650714311594498 2306 2 0.0976568721033928 2306 3 0.06666677347499927 2306 4 0.018198340653135858 2306 5 0.02067344858570408 2306 6 0.09517719358099441 2306 7 0.0894093365877211 2306 8 0.09118812051290348 2306 9 0.0892735837541565 2306 10-11 0.09920883488812393 2306 12-13 0.08126353684343712 2306 14-15 0.1315015993887627 2306 16-17 0.1639037422230416 2306 18-19 0.14798592722329573 2306 20-21 0.1250956809509205 2306 22-23 0.08398784812012394 2306 24-25 0.09803981435918274 2306 26-27 0.15633042480349246 2306 28-29 0.15519349784541703 2306 30-31 0.14887884946725904 2306 32-33 0.16136101956805504 2306 34-35 0.10244200887294852 2306 36-37 0.17095577474679402 2306 38-39 0.14131253092041618 2306 40-41 0.05294013658254215 2306 42-43 0.09129421494058221 2306 44-45 0.08641144238628584 2306 46-47 0.09898261368044103 2306 48-49 0.0467056556857699 2306 50-51 0.00989904936790964 2306 52-53 -0.016170578816236514 2306 54-55 -0.026276024710597312 2306 56-57 0.04270679097363228 2306 58-59 -0.006745965974971568 2306 60-61 -0.022339342532241346 2306 62-63 -0.010307107890263012 2306 64-65 -0.07653383159564697 2306 66-67 -0.14520599471340745 2306 68-69 -0.22678716586814573 2306 70-71 -0.24090984746613486 2306 72-73 -0.19960560085059598 2306 74-75 -0.250018941745914 2306 76-77 -0.5063403358718581 2306 78-79 -0.07660423514436587 2306 80-81 0.020274815563993798 2306 82-83 -0.019390648179033576 2306 84-85 -0.08290934189971466 2306 86-87 -0.19133514560208198 2306 88-89 -0.04939277520276164 2306 90-91 -0.1711915172039582 2306 92-93 -0.20252488619424014 2306 94-95 -0.14769901922139894 2306 96-97 -0.2678908812838543 2306 98-99 -0.20281143243488486 2306 100-101 -0.3221969250394423 2307 1 0.09214946096907539 2307 2 0.10016682514267217 2307 3 0.06135718471858809 2307 4 -0.002501730125239021 2307 5 0.029847170875505924 2307 6 0.08468385033905435 2307 7 0.07785178794594572 2307 8 0.11374036376855656 2307 9 0.09981002101763892 2307 10-11 0.08463409270421351 2307 12-13 0.1262009379658835 2307 14-15 0.15004628905196427 2307 16-17 0.15987786840113927 2307 18-19 0.12700426622179606 2307 20-21 0.1066048061116831 2307 22-23 0.12662186801096453 2307 24-25 0.11173805189055486 2307 26-27 0.13608742470883328 2307 28-29 0.08798088063402787 2307 30-31 0.08667564534481187 2307 32-33 0.10243340990781036 2307 34-35 0.09489008591528858 2307 36-37 0.11476220771788093 2307 38-39 0.0806536663775006 2307 40-41 0.0741018445012358 2307 42-43 0.10347461738175667 2307 44-45 0.0666873726513515 2307 46-47 0.04856643709464947 2307 48-49 -0.07558436483505915 2307 50-51 -0.016072521226583092 2307 52-53 -0.052709596416718796 2307 54-55 0.009060977746244703 2307 56-57 -0.018352245455744765 2307 58-59 0.010584199241677084 2307 60-61 -0.05560022912798246 2307 62-63 -0.02047634492003425 2307 64-65 -0.16443416329108373 2307 66-67 -0.2642938546984297 2307 68-69 -0.17224968229786697 2307 70-71 -0.18617222524967048 2307 72-73 -0.30077353587920186 2307 74-75 -0.33047588458244803 2307 76-77 -0.6733427702726544 2307 78-79 -0.17949502998391864 2307 80-81 -0.09093837192744303 2307 82-83 -0.14625424869847592 2307 84-85 -0.12348501872813955 2307 86-87 -0.11084701621312121 2307 88-89 -0.15978267443197325 2307 90-91 -0.1756796674021004 2307 92-93 -0.2033499595567676 2307 94-95 -0.1581567726634816 2307 96-97 -0.38666345855251194 2307 98-99 -0.23169516806975565 2307 100-101 -0.32808108488215737 2201 1 0.10002695715818533 2201 2 0.0968321816992983 2201 3 0.06496115209029085 2201 4 0.010619685665773204 2201 5 0.03658866163198837 2201 6 0.06784449751342692 2201 7 0.0755722167986832 2201 8 0.060144139650390116 2201 9 0.054430470210711235 2201 10-11 0.07075046707047505 2201 12-13 0.06334405453623049 2201 14-15 0.1703063645317826 2201 16-17 0.17757335645533345 2201 18-19 0.12506082267115914 2201 20-21 0.12355909110670638 2201 22-23 0.12007621714769101 2201 24-25 0.15382771421761277 2201 26-27 0.14528618818214767 2201 28-29 0.15387423379673493 2201 30-31 0.14176323991568296 2201 32-33 0.11202959326398343 2201 34-35 0.14475654655770853 2201 36-37 0.11548289001160583 2201 38-39 0.1431853052047103 2201 40-41 0.11791169016881753 2201 42-43 0.11566361533409975 2201 44-45 0.0763513730077463 2201 46-47 0.08827223132526996 2201 48-49 0.1537606906032778 2201 50-51 0.11596981157504871 2201 52-53 0.06435983929704747 2201 54-55 0.058241222086422795 2201 56-57 0.037268244168956244 2201 58-59 -0.035264562361263074 2201 60-61 0.03139017991097859 2201 62-63 0.040957159769526186 2201 64-65 0.012487763315782274 2201 66-67 -0.03860881754606993 2201 68-69 -0.08321434086384727 2201 70-71 -0.069828366953395 2201 72-73 -0.10286210952565256 2201 74-75 -0.14111164928043962 2201 76-77 -0.04044138295709132 2201 78-79 0.058639793554270625 2201 80-81 0.048887877925672285 2201 82-83 0.02835279262036039 2201 84-85 0.11364900771490483 2201 86-87 0.08575231366889113 2201 88-89 0.09404927991439394 2201 90-91 -0.034456401321641295 2201 92-93 -0.04965257441815396 2201 94-95 -0.008131136954311557 2201 96-97 -0.1178298303978309 2201 98-99 -0.02802588176749765 2201 100-101 -0.02939701740265832 1101 1 0.06992403133926928 1101 2 0.07538217732579255 1101 3 0.04252571330977872 1101 4 0.040972717744779175 1101 5 0.03457966819886593 1101 6 0.045397926722898774 1101 7 0.05056511799697461 1101 8 0.05241564256879627 1101 9 0.07364482845595433 1101 10-11 0.0862330423677804 1101 12-13 0.08610484273225438 1101 14-15 0.07956734169382429 1101 16-17 0.07159834624950889 1101 18-19 0.11177397272935252 1101 20-21 0.030110589702516677 1101 22-23 0.061943008473924976 1101 24-25 0.07069071201996735 1101 26-27 0.04721758896066319 1101 28-29 0.025864084433685264 1101 30-31 -0.00961476799070482 1101 32-33 0.035152435142450145 1101 34-35 0.05648324026551421 1101 36-37 -0.030412064412253415 1101 38-39 0.00399200131290911 1101 40-41 -0.03261756855294351 1101 42-43 0.06251958002604141 1101 44-45 0.07018503840757973 1101 46-47 0.062012663034209936 1101 48-49 -0.045773058088371954 1101 50-51 -0.0017878187046775906 1101 52-53 -0.061123790611738116 1101 54-55 0.0032065145978918963 1101 56-57 0.02819861507379784 1101 58-59 0.02597996321685514 1101 60-61 0.07119546324224046 1101 62-63 -0.049952778777758056 1101 64-65 -0.04921163595810896 1101 66-67 -0.14328279109603415 1101 68-69 -0.10307846583400249 1101 70-71 -0.16812371947828098 1101 72-73 -0.29536485761116893 1101 74-75 -0.2632177320687461 1101 76-77 -0.46281370187965365 1101 78-79 -0.07480585708478316 1101 80-81 -0.010663916340099888 1101 82-83 -0.05455587021396724 1101 84-85 -0.07075650803331257 1101 86-87 -0.03242251842476662 1101 88-89 0.03375415914361923 1101 90-91 -0.021776781516770427 1101 92-93 -0.12292892381484677 1101 94-95 -0.11279049567241373 1101 96-97 -0.13337602859447628 1101 98-99 -0.12163871199789611 1101 100-101 -0.07727206109328222 2304 1 0.08121808652875728 2304 2 0.08170250030815396 2304 3 0.042337436596426414 2304 4 -0.025893210498040276 2304 5 -0.017900911056784707 2304 6 0.03304727017874143 2304 7 0.03204885793459766 2304 8 0.06580640947658623 2304 9 0.033020888248671554 2304 10-11 0.05024461838604566 2304 12-13 0.05462458179108154 2304 14-15 0.07030645859850893 2304 16-17 0.03914948612763425 2304 18-19 0.048215282607124266 2304 20-21 0.06477561165306867 2304 22-23 0.06383780142981266 2304 24-25 0.02743197987584267 2304 26-27 0.06071234225050404 2304 28-29 0.06023870605857695 2304 30-31 0.08936668046617768 2304 32-33 0.08241832744477762 2304 34-35 0.059591408565665915 2304 36-37 0.07401512293143497 2304 38-39 0.0997146436627645 2304 40-41 -0.014368040682896321 2304 42-43 0.03882110820946849 2304 44-45 0.10143609648819307 2304 46-47 0.08783398013295596 2304 48-49 0.0672231988401748 2304 50-51 0.04650559656860764 2304 52-53 -0.01107452309115331 2304 54-55 0.037640457221300494 2304 56-57 0.030330418086997213 2304 58-59 0.03677096099850985 2304 60-61 -0.008926934427329059 2304 62-63 -0.006040844505037057 2304 64-65 -0.013811450009853843 2304 66-67 -0.07374711658984268 2304 68-69 -0.0237792754266124 2304 70-71 -0.07494381262772976 2304 72-73 -0.029578431833499508 2304 74-75 -0.1050315712426837 2304 76-77 -0.3584185925268457 2304 78-79 -0.12576056767337462 2304 80-81 0.12401629540202919 2304 82-83 0.09759928590531786 2304 84-85 0.06908627419959146 2304 86-87 0.10272205716232463 2304 88-89 0.17013816145783522 2304 90-91 0.12966083843327425 2304 92-93 -0.040465490259023795 2304 94-95 -0.035972939177078445 2304 96-97 -0.08171662946920577 2304 98-99 -0.012988776290846715 2304 100-101 0.1366980409043208 2202 1 0.0683085985057943 2202 2 0.0726597164099374 2202 3 0.03866763183135902 2202 4 -0.011285982447837739 2202 5 7.050445325802457E-4 2202 6 0.029098246231171743 2202 7 0.014925881500793992 2202 8 0.04316521389308292 2202 9 0.00592920689519616 2202 10-11 0.013122493519055922 2202 12-13 0.03788310677958151 2202 14-15 0.03970850437573148 2202 16-17 0.022476317266217904 2202 18-19 -0.002678933848521581 2202 20-21 -0.008565995086449618 2202 22-23 0.04666680976803406 2202 24-25 -0.01741106240567092 2202 26-27 -0.02578211115666562 2202 28-29 -0.04998887873700397 2202 30-31 -0.01790808877041883 2202 32-33 -0.004888203400376767 2202 34-35 0.024086522140841282 2202 36-37 0.023827299481190778 2202 38-39 0.04673425913756546 2202 40-41 0.005177513455869587 2202 42-43 0.04797832649109779 2202 44-45 0.002792710666689402 2202 46-47 0.058376860596396796 2202 48-49 -0.00992666357473837 2202 50-51 -0.027363400980384256 2202 52-53 -0.01623179912856898 2202 54-55 0.053217850078198126 2202 56-57 0.03139882630286195 2202 58-59 -0.01065942850773638 2202 60-61 -0.005909933038545034 2202 62-63 -0.039780168597310706 2202 64-65 -0.025510246346648557 2202 66-67 -0.07790305039090839 2202 68-69 -0.024843211898094353 2202 70-71 -0.04103157259752521 2202 72-73 -0.16045132448576283 2202 74-75 -0.13724814668508856 2202 76-77 -0.37057856933489575 2202 78-79 -0.014249913105743417 2202 80-81 0.11798983041681055 2202 82-83 0.09794636304484783 2202 84-85 0.0669523735692863 2202 86-87 0.028841057716249452 2202 88-89 0.060621436318960775 2202 90-91 0.049718514258891844 2202 92-93 0.09667309297012139 2202 94-95 -0.001964551549562543 2202 96-97 -0.08002271436227204 2202 98-99 0.06418714753181831 2202 100-101 0.15008576110717087 1102 1 0.07127404551007999 1102 2 0.07009605722897305 1102 3 0.03820758590929074 1102 4 -0.014595678412483437 1102 5 -0.013317024952563372 1102 6 0.03238008127270575 1102 7 0.02554766916664164 1102 8 0.0401233386227986 1102 9 0.023416100147883867 1102 10-11 0.05374831874748054 1102 12-13 0.05617266717055003 1102 14-15 0.09074668716519341 1102 16-17 0.04712464124998661 1102 18-19 0.031098460706964204 1102 20-21 -0.004627781680262899 1102 22-23 0.032476779799374356 1102 24-25 0.018330508302327075 1102 26-27 0.04818506246775911 1102 28-29 0.04855126945888344 1102 30-31 -0.03953628490284444 1102 32-33 0.0038599939676089434 1102 34-35 0.03127144159854822 1102 36-37 0.008870261327480478 1102 38-39 0.028054040150706783 1102 40-41 -0.03371430999914793 1102 42-43 -0.03127398096872014 1102 44-45 -0.06998176182306537 1102 46-47 -0.03304957248468554 1102 48-49 -0.061437251785456226 1102 50-51 -0.05120398532084636 1102 52-53 -0.0732120430085601 1102 54-55 -0.10475118023448715 1102 56-57 -0.04817865144170952 1102 58-59 -0.12491654424806597 1102 60-61 -0.027829173610349756 1102 62-63 -0.015253906497491698 1102 64-65 -0.11861617069940422 1102 66-67 -0.1056746368524415 1102 68-69 -0.09709977783944623 1102 70-71 -0.18785347028374133 1102 72-73 -0.16825741760907675 1102 74-75 -0.09022666863888418 1102 76-77 -0.36357359131474 1102 78-79 -0.0883129722169329 1102 80-81 0.05001387197076923 1102 82-83 0.03844726080252059 1102 84-85 0.012212700583852154 1102 86-87 0.04574858761525746 1102 88-89 -0.0258047560074246 1102 90-91 -0.1221387887552865 1102 92-93 -0.17058701388257447 1102 94-95 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44-45 -0.17372607020120512 1213 46-47 -0.3796869233909774 1213 48-49 -0.23548269394489552 1213 50-51 -0.18648449731361438 1213 52-53 -0.15189231664151492 1213 54-55 -0.07997826808573905 1213 56-57 -0.08956966659523857 1213 58-59 -0.1471670699449703 1213 60-61 -0.11495108273013699 1213 62-63 -0.09017783195876916 1213 64-65 -0.03765415794482152 1213 66-67 0.023387265214182662 1213 68-69 0.06700948105315518 1213 70-71 0.0076203934577492305 1213 72-73 0.023458292620134102 1213 74-75 0.07226162864065344 1213 76-77 0.46260033254687016 1213 78-79 -2.702765518378669E-4 1213 80-81 -0.1565614709708143 1213 82-83 -0.11912264312775989 1213 84-85 -0.066248818330088 1213 86-87 -0.09126205767837092 1213 88-89 -0.022000440621816608 1213 90-91 -0.06681026430083392 1213 92-93 0.0063707911486901025 1213 94-95 0.09714506662923128 1213 96-97 0.03724512645816702 1213 98-99 0.143508283628897 1213 100-101 0.3702987326654821 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 5.0 7 41.0 8 484.0 9 1349.0 10 944.0 11 290.0 12 236.0 13 377.0 14 873.0 15 2103.0 16 3815.0 17 5987.0 18 8527.0 19 11514.0 20 15079.0 21 19801.0 22 25295.0 23 32819.0 24 41942.0 25 54086.0 26 69356.0 27 88901.0 28 115462.0 29 149051.0 30 194420.0 31 259121.0 32 356375.0 33 515785.0 34 830549.0 35 1635335.0 36 4001505.0 37 6526102.0 38 1329940.0 39 164.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.91631313266304 23.052850222934566 31.343349336449776 25.687487307952615 2 26.69258167735155 23.608152177681262 30.478088443886296 19.221177701080887 3 20.877964303159853 21.610849870039413 37.25888293103667 20.252302895764064 4 20.668655490647957 21.97748649613925 36.32256077582577 21.031297237387022 5 27.424332110067763 21.259130083491264 31.75766689555471 19.558870910886263 6 28.527375723824434 21.92123850132102 30.691297319064674 18.86008845578987 7 27.433063439335026 27.52111303524874 25.533210865651473 19.512612659764763 8 25.32965369879172 23.36066225076979 30.779181246749143 20.530502803689345 9 22.151394622765157 22.26630087939764 34.188424785366074 21.393879712471133 10-11 26.492539545713743 23.188235103182546 28.4778717127741 21.84135363832961 12-13 25.468410044575187 26.523584743870476 25.469542110808362 22.53846310074598 14-15 25.140585617143984 24.81738350482545 26.697857259702108 23.344173618328455 16-17 24.70692216470944 24.656911834988556 26.27932227949912 24.356843720802893 18-19 24.76493979217718 25.128492585395684 26.765757947795244 23.340809674631892 20-21 23.601941505108258 25.549070331576022 27.267332541901613 23.581655621414114 22-23 23.927529844364518 25.718369655274483 26.73882458882219 23.61527591153881 24-25 24.254430075827578 25.570124201471465 26.473684859635753 23.701760863065207 26-27 24.053502125124552 25.559595065123876 26.778925504090072 23.607977305661503 28-29 24.228604239646334 26.044269741501726 26.181574956314208 23.545551062537733 30-31 24.34771473436864 25.894734717158773 26.05077279976517 23.70677774870742 32-33 24.275552775056354 26.0708594922956 26.131951185794893 23.521636546853152 34-35 23.937076412161947 26.199386860961837 26.212950200638335 23.65058652623788 36-37 24.137240748542393 25.892018171516607 26.396233701735362 23.57450737820564 38-39 24.27531406123 25.76478591210418 26.2207311538793 23.73916887278651 40-41 23.906684169533996 26.055919482719396 26.05578448339209 23.981611864354516 42-43 23.95927931997537 25.920841632551706 26.24463731897994 23.875241728492984 44-45 24.195824620308464 25.943191077862537 26.00306244416301 23.85792185766599 46-47 23.653889005421416 26.241472557172703 26.203825238761034 23.900813198644844 48-49 23.967725587358128 25.863202143699972 26.101700208739114 24.06737206020279 50-51 23.81880876963935 26.09073398015122 26.409093063635154 23.68136418657427 52-53 24.015844170716562 25.757366911561956 26.614606804391705 23.612182113329773 54-55 23.813073884676662 26.021087516544426 25.961503941236565 24.204334657542343 56-57 24.009818100506436 26.06708200692756 26.345693577106516 23.57740631545949 58-59 23.70496725766187 26.27170531690049 26.134659569825764 23.888667855611875 60-61 24.249623438996657 26.07528421691374 25.756441242077365 23.91865110201223 62-63 23.725866561683738 26.440351634720283 26.250092576951467 23.583689226644513 64-65 23.921023531807446 26.33094047427252 26.162011298824172 23.586024695095862 66-67 23.83479704344498 26.442127236262703 26.346413810250112 23.376661910042206 68-69 23.827419693148354 26.255457655954896 26.616890849642814 23.30023180125394 70-71 23.56803502218388 26.45274189490823 26.46637736999607 23.512845712911822 72-73 23.69687480582126 26.02584140285846 26.53962550448117 23.73765828683911 74-75 23.845294930984593 25.938224860139396 26.57072993413161 23.6457502747444 76-77 23.944867950132398 25.93102829044045 26.13182521194915 23.992278547477998 78-79 24.056298732592467 25.785484347381782 26.334531561907653 23.823685358118098 80-81 23.8913604413204 25.890814313458982 26.289527538037195 23.92829770718342 82-83 23.784813814378765 26.467205871290144 26.302264823862647 23.445715490468444 84-85 23.81721765085442 26.2559055499865 26.200288344143374 23.726588455015698 86-87 23.826671591755883 26.478935839544693 26.166358971319088 23.528033597380336 88-89 23.800510991496495 27.053654966260932 25.76033756895162 23.385496473290953 90-91 23.701307351234085 26.3263688146056 26.06630432180349 23.906019512356824 92-93 23.570889477348288 26.58816212884429 25.886272684764105 23.954675709043315 94-95 23.578078604019154 26.726079709536055 25.92215250989837 23.77368917654642 96-97 23.49840790610976 26.113110873699615 26.001224550694786 24.38725666949584 98-99 24.02000737649481 26.110606543916997 25.898052029637817 23.97133404995037 100-101 24.18582712772288 26.64226350554862 25.392337546007344 23.779571820721156 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3326.0 1 2158.0 2 1353.0 3 1560.0 4 1454.5 5 1256.0 6 976.5 7 959.0 8 1013.5 9 1038.0 10 1071.0 11 1160.0 12 1236.0 13 1310.5 14 1504.5 15 1728.0 16 1913.0 17 2235.5 18 2742.0 19 3401.0 20 4260.0 21 5577.0 22 7413.5 23 9869.0 24 13370.5 25 17448.5 26 21861.0 27 27099.5 28 33577.5 29 41227.5 30 50699.5 31 63856.0 32 80113.0 33 98822.5 34 122561.0 35 152533.5 36 183727.5 37 217999.0 38 259300.0 39 302193.5 40 351915.0 41 408818.0 42 453370.5 43 499214.0 44 572018.0 45 681166.5 46 881746.5 47 1121223.0 48 1270749.5 49 1292369.5 50 1209245.0 51 1090991.5 52 929478.0 53 767235.0 54 644580.0 55 550082.0 56 482006.5 57 382220.0 58 278424.5 59 217867.0 60 171286.0 61 115085.0 62 71545.5 63 45255.5 64 24916.0 65 13241.5 66 7856.5 67 4765.0 68 3490.0 69 2579.5 70 1903.0 71 1452.0 72 1152.5 73 838.5 74 611.0 75 451.0 76 307.0 77 227.0 78 185.0 79 138.0 80 97.5 81 71.0 82 53.5 83 40.5 84 26.5 85 16.5 86 10.0 87 10.5 88 11.0 89 9.0 90 7.0 91 7.0 92 7.0 93 3.5 94 2.5 95 3.5 96 3.5 97 3.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1391490408453792 2 0.0 3 0.0 4 0.18446236947414388 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.191373557129431E-4 12-13 0.0 14-15 5.583632911601335E-4 16-17 0.0 18-19 0.0 20-21 0.003595614160657563 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 5.522274308177144E-5 32-33 0.0 34-35 1.503285783892667E-4 36-37 2.3929855335434294E-4 38-39 0.009127092259348336 40-41 0.0077035726599071165 42-43 0.0010093490263279336 44-45 0.0011842210460868766 46-47 0.0019849508207725624 48-49 1.0124169564991432E-4 50-51 9.541262832461621E-4 52-53 0.019548851050947093 54-55 0.01763446262411235 56-57 0.02112269922877758 58-59 0.04124832115191206 60-61 0.015121827813891747 62-63 0.002331626930119239 64-65 0.007108394206692468 66-67 0.01296814083370266 68-69 0.028105308298450454 70-71 0.01168574602213708 72-73 0.018686762672837216 74-75 0.012471136145966719 76-77 0.023362288253760532 78-79 0.009163907421402849 80-81 0.006645136750839831 82-83 0.0018100788010136195 84-85 0.019726791000877243 86-87 0.009090277097293821 88-89 0.012630668514869613 90-91 0.04566920852862499 92-93 0.035287332829251956 94-95 0.02946440136429627 96-97 0.0264455580758261 98-99 0.032777765949202564 100-101 0.24316414537006695 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6297633E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.7996272657051 #Duplication Level Percentage of deduplicated Percentage of total 1 77.77633734901833 21.621531883944485 2 10.973501707362995 6.101185145285396 3 3.4845723415612144 2.90609436827361 4 1.997187781102055 2.2208430357703106 5 1.1295984082548995 1.5701207354709996 6 0.7741886659554836 1.2913293808137554 7 0.5142688919850597 1.0007538458072271 8 0.3140261825148069 0.698384866046713 9 0.3397032404535747 0.8499261119005412 >10 1.9212318200474132 10.40508752308566 >50 0.28784519147469156 5.7088632120342035 >100 0.40539211480471965 24.602287996824774 >500 0.06081762245791181 11.573208608093069 >1k 0.02123637604056214 9.296349008430933 >5k 9.23069662218113E-5 0.15403427821831508 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.522274308177144E-5 0.0 0.0 2 6.135860342419049E-5 0.0 0.0 3 6.135860342419049E-5 0.0 0.0 4 6.749446376660955E-5 0.0 0.0 5 8.590204479386669E-5 0.0 0.0 6 9.203790513628575E-5 0.0 0.0 7 9.203790513628575E-5 0.0 0.0 8 1.0430962582112384E-4 0.0 0.0 9 1.2271720684838098E-4 0.0 0.0 10-11 1.5953236890289528E-4 0.0 0.0 12-13 2.1475511198466672E-4 0.0 0.0 14-15 3.466761093466763E-4 0.0 0.0 16-17 4.7552917653747635E-4 0.0 0.0 18-19 6.289256850979526E-4 0.0 0.0 20-21 8.682242384522955E-4 0.0 0.0 22-23 0.0010676396995809147 0.0 0.0 24-25 0.0013560251356746099 0.0 0.0 26-27 0.001607595409713791 0.0 0.0 28-29 0.001981882890601353 0.0 0.0 30-31 0.0023837817430298006 0.0 0.0 32-33 0.002859310919567277 0.0 0.0 34-35 0.0034299459314122488 0.0 0.0 36-37 0.003997513013086011 0.0 0.0 38-39 0.004718476603320249 0.0 0.0 40-41 0.005525342238348354 0.0 0.0 42-43 0.006574574356902011 0.0 0.0 44-45 0.007660621637510183 0.0 0.0 46-47 0.008979831611130279 0.0 0.0 48-49 0.010707076297521241 0.0 6.1358603424190496E-6 50-51 0.012688959188122594 0.0 6.1358603424190496E-6 52-53 0.015327379135362786 0.0 1.2271720684838099E-5 54-55 0.018419852747941987 0.0 1.2271720684838099E-5 56-57 0.022481792294623396 0.0 1.2271720684838099E-5 58-59 0.027878281465780953 0.0 1.2271720684838099E-5 60-61 0.03436695377788909 0.0 1.2271720684838099E-5 62-63 0.042699452122894166 0.0 1.2271720684838099E-5 64-65 0.05305371645072631 0.0 1.2271720684838099E-5 66-67 0.06622740860590001 0.0 1.2271720684838099E-5 68-69 0.08295989975967677 0.0 1.2271720684838099E-5 70-71 0.10425133514787085 0.0 1.2271720684838099E-5 72-73 0.13147614748718417 0.0 1.2271720684838099E-5 74-75 0.16522337937048898 0.0 1.2271720684838099E-5 76-77 0.20738594371342145 0.0 1.2271720684838099E-5 78-79 0.26095507243291094 0.0 1.2271720684838099E-5 80-81 0.32586327106519086 0.0 1.2271720684838099E-5 82-83 0.40686889930580716 0.0 1.2271720684838099E-5 84-85 0.5069325097699771 0.0 1.2271720684838099E-5 86-87 0.6265848543773197 0.0 1.2271720684838099E-5 88-89 0.7699062802555439 0.0 1.2271720684838099E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 126965 0.0 70.74824 1 TTTGTGT 138110 0.0 65.1211 2 TTGTGTT 156995 0.0 58.84498 3 TGTGTTT 169750 0.0 54.782047 4 GTGTTTG 185425 0.0 50.140232 5 ACTTTGT 33495 0.0 49.68484 1 AACTTTG 16725 0.0 45.533024 2 TAACTTT 17150 0.0 44.186237 1 TTTGAGG 51330 0.0 33.864777 8 GTTTGAG 142525 0.0 33.27403 2 TTTGAGT 54660 0.0 33.2339 3 TGTTTGA 296140 0.0 32.15599 1 TTGAGGG 26155 0.0 31.569664 9 GTTTGAA 28080 0.0 31.236694 2 TTTGAGC 38930 0.0 29.149645 8 TTGAGGT 14435 0.0 26.87388 9 TTGACCC 8485 0.0 26.804565 9 GAGGGCG 6265 0.0 26.601482 6 GTTTGAC 67835 0.0 26.297058 7 GTTTGAT 91660 0.0 26.18597 2 >>END_MODULE