FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l05n01_30191.34100000035b8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l05n01_30191.34100000035b8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16297633
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1269650.070.748241
TTTGTGT1381100.065.12112
TTGTGTT1569950.058.844983
TGTGTTT1697500.054.7820474
GTGTTTG1854250.050.1402325
ACTTTGT334950.049.684841
AACTTTG167250.045.5330242
TAACTTT171500.044.1862371
TTTGAGG513300.033.8647778
GTTTGAG1425250.033.274032
TTTGAGT546600.033.23393
TGTTTGA2961400.032.155991
TTGAGGG261550.031.5696649
GTTTGAA280800.031.2366942
TTTGAGC389300.029.1496458
TTGAGGT144350.026.873889
TTGACCC84850.026.8045659
GAGGGCG62650.026.6014826
GTTTGAC678350.026.2970587
GTTTGAT916600.026.185972