##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l05n01_30112.34100000035b54.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12181520 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74484440365406 33.0 33.0 33.0 33.0 33.0 2 32.760017961633686 33.0 33.0 33.0 33.0 33.0 3 32.795557861416306 33.0 33.0 33.0 33.0 33.0 4 36.6622352547137 37.0 37.0 37.0 37.0 37.0 5 36.70321248908182 37.0 37.0 37.0 37.0 37.0 6 36.671689493593576 37.0 37.0 37.0 37.0 37.0 7 36.64089374724993 37.0 37.0 37.0 37.0 37.0 8 36.63551322002509 37.0 37.0 37.0 37.0 37.0 9 36.625737838956056 37.0 37.0 37.0 37.0 37.0 10-11 36.59895374304685 37.0 37.0 37.0 37.0 37.0 12-13 36.5732753794272 37.0 37.0 37.0 37.0 37.0 14-15 38.67766867353171 40.0 37.0 40.0 37.0 40.0 16-17 38.63284754283538 40.0 37.0 40.0 37.0 40.0 18-19 38.58173573577025 40.0 37.0 40.0 37.0 40.0 20-21 38.54865176923734 40.0 37.0 40.0 37.0 40.0 22-23 38.50091417163047 40.0 37.0 40.0 37.0 40.0 24-25 38.443945049550464 40.0 37.0 40.0 37.0 40.0 26-27 38.34920046923537 40.0 37.0 40.0 37.0 40.0 28-29 38.26301463199995 40.0 37.0 40.0 37.0 40.0 30-31 38.20252489016149 40.0 37.0 40.0 37.0 40.0 32-33 38.12482518601948 40.0 37.0 40.0 37.0 40.0 34-35 38.03025332635008 40.0 37.0 40.0 37.0 40.0 36-37 37.92008517820436 40.0 37.0 40.0 35.0 40.0 38-39 37.798203344081855 40.0 37.0 40.0 33.0 40.0 40-41 37.673948940690494 40.0 37.0 40.0 33.0 40.0 42-43 37.512587304375806 40.0 37.0 40.0 33.0 40.0 44-45 37.38178761763721 40.0 37.0 40.0 33.0 40.0 46-47 37.17738816666557 38.5 37.0 40.0 33.0 40.0 48-49 36.95227804083562 37.0 37.0 40.0 33.0 40.0 50-51 36.73466697916187 37.0 37.0 40.0 33.0 40.0 52-53 36.48374800517505 37.0 37.0 40.0 33.0 40.0 54-55 36.21651230716692 37.0 37.0 40.0 33.0 40.0 56-57 35.94510635782727 37.0 37.0 40.0 33.0 40.0 58-59 35.673567912707114 37.0 35.0 38.5 33.0 40.0 60-61 35.38445760463391 37.0 33.0 37.0 33.0 40.0 62-63 35.137000801213645 37.0 33.0 37.0 33.0 40.0 64-65 34.8724355417058 37.0 33.0 37.0 27.0 40.0 66-67 34.60858612061549 37.0 33.0 37.0 27.0 38.5 68-69 34.34489587506321 37.0 33.0 37.0 27.0 37.0 70-71 34.12650638015617 37.0 33.0 37.0 27.0 37.0 72-73 33.94958506820167 37.0 33.0 37.0 27.0 37.0 74-75 33.68403922498999 37.0 33.0 37.0 27.0 37.0 76-77 30.73800580715707 33.0 30.0 33.0 24.5 37.0 78-79 33.4376057339314 35.0 33.0 37.0 27.0 37.0 80-81 33.942531309721616 37.0 33.0 37.0 27.0 37.0 82-83 33.914808702033895 37.0 33.0 37.0 27.0 37.0 84-85 33.835616244934954 37.0 33.0 37.0 27.0 37.0 86-87 33.696071631454856 37.0 33.0 37.0 27.0 37.0 88-89 33.57817382395629 37.0 33.0 37.0 27.0 37.0 90-91 33.482565927733155 37.0 33.0 37.0 27.0 37.0 92-93 33.38829850462011 37.0 33.0 37.0 27.0 37.0 94-95 33.25187525858841 37.0 33.0 37.0 27.0 37.0 96-97 33.14199874071545 37.0 33.0 37.0 27.0 37.0 98-99 32.97624487748655 35.0 33.0 37.0 27.0 37.0 100-101 31.10055658078795 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.07290617627288043 2306 2 0.06923374263853077 2306 3 0.050178416499512934 2306 4 0.04862678470465198 2306 5 0.015171520416480178 2306 6 0.05107745200022862 2306 7 0.10229317274478689 2306 8 0.10015328776596277 2306 9 0.07139756827876198 2306 10-11 0.09541175088128284 2306 12-13 0.09771361471655382 2306 14-15 0.11974940776745058 2306 16-17 0.19391920934666018 2306 18-19 0.17595632149269846 2306 20-21 0.138874243930033 2306 22-23 0.1325941636494008 2306 24-25 0.1040085997421798 2306 26-27 0.1440385069114285 2306 28-29 0.08516114776870864 2306 30-31 0.1138066635270718 2306 32-33 0.07930164104271142 2306 34-35 0.08923755436396874 2306 36-37 0.12275393038136428 2306 38-39 0.17509758982706813 2306 40-41 1.4147594912117256E-4 2306 42-43 0.03797811038236887 2306 44-45 0.08641261368101993 2306 46-47 0.11324493465994578 2306 48-49 -0.010787038403762494 2306 50-51 -0.08377543468282767 2306 52-53 -0.062170646056493695 2306 54-55 -0.05288223181065632 2306 56-57 -0.03602718850724074 2306 58-59 -0.09822779943944937 2306 60-61 -0.1274494035039666 2306 62-63 -0.042690964985666824 2306 64-65 -0.08764731397914716 2306 66-67 -0.19194769622824737 2306 68-69 -0.3062473765733529 2306 70-71 -0.34253365037392314 2306 72-73 -0.29426754662954835 2306 74-75 -0.3660171751751662 2306 76-77 -0.48786470273374505 2306 78-79 -0.13333838960666355 2306 80-81 -0.043977075803667276 2306 82-83 -0.12369357389857072 2306 84-85 -0.19989903353924632 2306 86-87 -0.2293607068086274 2306 88-89 -0.14600033295685932 2306 90-91 -0.23918744176393147 2306 92-93 -0.3278586053381076 2306 94-95 -0.3300153344013168 2306 96-97 -0.4140143117596935 2306 98-99 -0.2922891258131415 2306 100-101 -0.4724972447332014 2307 1 0.08274120738902724 2307 2 0.08983493084753746 2307 3 0.0887713128872818 2307 4 0.01725569910651359 2307 5 0.03849585210345907 2307 6 0.10668677431465312 2307 7 0.10218249633922483 2307 8 0.1303729075731539 2307 9 0.09940615350546267 2307 10-11 0.09035325992044108 2307 12-13 0.10657489606309412 2307 14-15 0.14724468927980894 2307 16-17 0.1368073009290569 2307 18-19 0.16624172012412686 2307 20-21 0.12574220658858337 2307 22-23 0.12738020363774183 2307 24-25 0.057048829433774983 2307 26-27 0.13829423003225827 2307 28-29 0.11233185468262974 2307 30-31 0.11863768473392611 2307 32-33 0.11504997718230925 2307 34-35 0.09995877595304137 2307 36-37 0.13576799254467176 2307 38-39 0.06253612688914245 2307 40-41 0.0873629511510643 2307 42-43 0.05318346667363727 2307 44-45 0.038772538027288306 2307 46-47 0.0851612448036505 2307 48-49 -0.0020913430899582863 2307 50-51 0.0038176415508459627 2307 52-53 -0.08161586193704551 2307 54-55 -0.0042516010283932815 2307 56-57 -0.05805988420317476 2307 58-59 -0.03642171530730565 2307 60-61 -0.1375820479162897 2307 62-63 -0.08422417580352004 2307 64-65 -0.1459524028807948 2307 66-67 -0.24787728141068754 2307 68-69 -0.2040223002972823 2307 70-71 -0.3035396331856006 2307 72-73 -0.3919744489099486 2307 74-75 -0.3460772401773582 2307 76-77 -0.5496146304733003 2307 78-79 -0.15141699473797843 2307 80-81 -0.015669305836873093 2307 82-83 -0.1501138708864076 2307 84-85 -0.16121031803386643 2307 86-87 -0.1592290608476148 2307 88-89 -0.21507930629956462 2307 90-91 -0.24861372862844888 2307 92-93 -0.25082219064481137 2307 94-95 -0.206078591538585 2307 96-97 -0.47871659300793823 2307 98-99 -0.28866492158715573 2307 100-101 -0.41098269591578784 2201 1 0.06943448416450337 2201 2 0.06754160673823151 2201 3 0.060010548992067925 2201 4 -0.02339541414877999 2201 5 -0.0020410227713796303 2201 6 0.004672355701529796 2201 7 0.10834909321882691 2201 8 0.06945244661446281 2201 9 0.0352997616177575 2201 10-11 0.07703879943508696 2201 12-13 0.058893343513439333 2201 14-15 0.152294844935291 2201 16-17 0.1871272594209259 2201 18-19 0.11637666705561145 2201 20-21 0.0880146400936539 2201 22-23 0.06937964837549515 2201 24-25 0.13577568592123868 2201 26-27 0.1054174709432587 2201 28-29 0.10453451630034749 2201 30-31 0.08607906360482787 2201 32-33 0.07559199505993064 2201 34-35 0.1476817456047712 2201 36-37 0.12069740141794227 2201 38-39 0.08930522882072722 2201 40-41 0.15347921032198997 2201 42-43 0.04786637551960382 2201 44-45 0.14235397952450057 2201 46-47 0.056372945362547 2201 48-49 0.12008774263889421 2201 50-51 0.07006020012646275 2201 52-53 -0.004308110111956864 2201 54-55 0.015945835662613206 2201 56-57 0.061838757679986145 2201 58-59 -0.012666048901579074 2201 60-61 0.06902329716554334 2201 62-63 -0.006995844685164343 2201 64-65 0.01548781995231252 2201 66-67 -0.07411751880805895 2201 68-69 -0.04681253860492518 2201 70-71 -0.0050165397376886744 2201 72-73 -0.07940048979909164 2201 74-75 -0.1398555722059811 2201 76-77 -0.03115034020768448 2201 78-79 0.07081147292026913 2201 80-81 0.06855282637015847 2201 82-83 0.1072126324178555 2201 84-85 0.07342029603156419 2201 86-87 0.07054847014694587 2201 88-89 0.052152256263021 2201 90-91 0.024409856363078575 2201 92-93 -0.034318613299518574 2201 94-95 -0.03180999025328646 2201 96-97 -0.11879947272539226 2201 98-99 -0.036478690270357106 2201 100-101 -0.08886914933748002 1101 1 0.08694937952778048 1101 2 0.09034002308093392 1101 3 0.0686908190283333 1101 4 0.0167116241726859 1101 5 0.014980961587909292 1101 6 0.021200962726638295 1101 7 0.01846566022037166 1101 8 0.045858781865447895 1101 9 0.0380055647860047 1101 10-11 0.08683080062888848 1101 12-13 0.05186831246362544 1101 14-15 0.09277813399317125 1101 16-17 0.055110972062053065 1101 18-19 0.0756480105995081 1101 20-21 0.042874033771148845 1101 22-23 0.022594140052277112 1101 24-25 0.08971267849570808 1101 26-27 0.10595538280996664 1101 28-29 0.02732803617612234 1101 30-31 -0.03271248835756069 1101 32-33 0.06989040907097888 1101 34-35 -0.0018202137592169265 1101 36-37 0.006474097655129185 1101 38-39 -0.03700434291717869 1101 40-41 -0.08992695860456479 1101 42-43 0.016905937417938333 1101 44-45 0.07490825647177246 1101 46-47 0.022550405759460546 1101 48-49 -0.02802991557528145 1101 50-51 -0.03072782016200648 1101 52-53 -0.07321810994097433 1101 54-55 -0.10358189621619118 1101 56-57 -0.050389663279382546 1101 58-59 -0.01683504074235742 1101 60-61 0.016174874682832296 1101 62-63 -0.13934237153568318 1101 64-65 -0.10181831493213878 1101 66-67 -0.17433700488111015 1101 68-69 -0.1551577504043138 1101 70-71 -0.22313556585373817 1101 72-73 -0.2768213729973752 1101 74-75 -0.2797627103221032 1101 76-77 -0.5088965197976556 1101 78-79 -0.043891606879697065 1101 80-81 0.042350588296166336 1101 82-83 0.007996999786556103 1101 84-85 -0.06610274145672435 1101 86-87 -0.07298609502348086 1101 88-89 -0.03133102813155375 1101 90-91 -0.04556244362401429 1101 92-93 -0.10386600876771723 1101 94-95 -0.1916191679421857 1101 96-97 -0.2038165948153008 1101 98-99 -0.14333180092584996 1101 100-101 -0.07499582637739977 2304 1 0.08349249014011662 2304 2 0.06847013101551624 2304 3 0.06590112206152554 2304 4 -0.00633992563747654 2304 5 -0.00850688412021583 2304 6 0.04929051157466091 2304 7 0.05383037819623837 2304 8 0.0729664245004571 2304 9 0.02661681147150574 2304 10-11 0.07352993004862896 2304 12-13 0.0516357072634861 2304 14-15 0.09114206018641369 2304 16-17 0.08160641201175878 2304 18-19 0.08195778731713688 2304 20-21 0.06403624697350807 2304 22-23 0.06017876229269348 2304 24-25 -0.013043001469732474 2304 26-27 0.0496947341440972 2304 28-29 0.06530951661132178 2304 30-31 0.09957378973025044 2304 32-33 0.08415704039906302 2304 34-35 0.06536638224693547 2304 36-37 0.06344329487005496 2304 38-39 0.10664079174920715 2304 40-41 -0.0034027199742610037 2304 42-43 0.02050820761358807 2304 44-45 0.06697620837613272 2304 46-47 0.07486231155032641 2304 48-49 0.012947346719009545 2304 50-51 0.027155964452084902 2304 52-53 -0.07231215289662885 2304 54-55 -0.07468844542054143 2304 56-57 0.0032226379771529423 2304 58-59 -0.04414955610286597 2304 60-61 -0.02508804877471249 2304 62-63 -0.07778648093842122 2304 64-65 -0.14511508611944635 2304 66-67 -0.20802725497026842 2304 68-69 -0.13628216684755046 2304 70-71 -0.18300677947356547 2304 72-73 -0.1738131362113009 2304 74-75 -0.1861224551058811 2304 76-77 -0.47143048691996725 2304 78-79 -0.22974244046147874 2304 80-81 0.06233894534896933 2304 82-83 0.023809068290553626 2304 84-85 0.005539123026515824 2304 86-87 0.0832259054526574 2304 88-89 0.11846510927973952 2304 90-91 0.033816065853237376 2304 92-93 -0.12418899030677721 2304 94-95 -0.15734424840400862 2304 96-97 -0.11983450469950441 2304 98-99 -0.060615850878079414 2304 100-101 0.09329329924661423 2202 1 0.06544208104369886 2202 2 0.05334522378687012 2202 3 0.04072859171250087 2202 4 -0.02385487752349036 2202 5 -0.022130179006126127 2202 6 0.049359828883041246 2202 7 0.02526582145031142 2202 8 0.036802246028713625 2202 9 0.005017579457373245 2202 10-11 0.028806358590500736 2202 12-13 0.043389358521508825 2202 14-15 0.03241572168339957 2202 16-17 0.028152086812944788 2202 18-19 0.00529215004392114 2202 20-21 0.013268959530897462 2202 22-23 0.08199177192535956 2202 24-25 0.019512450758718103 2202 26-27 0.003379688319022023 2202 28-29 -0.02478315722462554 2202 30-31 0.0537733562029743 2202 32-33 0.05067937433919667 2202 34-35 0.010008110686818839 2202 36-37 0.07601234094597942 2202 38-39 0.10570276130093958 2202 40-41 0.06530968724136699 2202 42-43 0.08214766955855168 2202 44-45 0.09024400903794572 2202 46-47 0.1341941281582848 2202 48-49 0.11546947044636369 2202 50-51 0.07068083148063664 2202 52-53 0.11489132130300561 2202 54-55 0.08251046100394177 2202 56-57 0.14206632301885946 2202 58-59 0.07764762471745001 2202 60-61 0.11063014237882385 2202 62-63 0.16085411348944234 2202 64-65 0.07119863217927502 2202 66-67 -0.016528692157571356 2202 68-69 0.016856550238884438 2202 70-71 0.03022331769458475 2202 72-73 -0.12088512160281795 2202 74-75 -0.09299550498199238 2202 76-77 -0.3112945398147069 2202 78-79 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34-35 0.02428583573907872 1102 36-37 -0.050040001094544095 1102 38-39 -0.031451799644308664 1102 40-41 -0.07373660895623146 1102 42-43 -0.0465423534177134 1102 44-45 -0.039339662183607516 1102 46-47 -0.04306843158570217 1102 48-49 -0.07117451614737291 1102 50-51 -0.05957120050064901 1102 52-53 -0.08536117359835771 1102 54-55 -0.08372164922331393 1102 56-57 -0.029984381191326293 1102 58-59 -0.15115200562094344 1102 60-61 -0.11055117064989162 1102 62-63 -0.1402097372466784 1102 64-65 -0.20447018947886875 1102 66-67 -0.24003699370999954 1102 68-69 -0.14627348788289396 1102 70-71 -0.2004674672028841 1102 72-73 -0.21310502142471677 1102 74-75 -0.1886742419563845 1102 76-77 -0.5056873534574926 1102 78-79 -0.13298230755394513 1102 80-81 -0.012401390757617037 1102 82-83 0.010013664354922014 1102 84-85 -0.02596062849801939 1102 86-87 -0.03255857260614903 1102 88-89 -0.14117688929047034 1102 90-91 -0.22634530407786002 1102 92-93 -0.24389486431749674 1102 94-95 -0.38414153489180336 1102 96-97 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44-45 -0.19830676059525842 1213 46-47 -0.3578391237183993 1213 48-49 -0.22555074382116658 1213 50-51 -0.12277365570940901 1213 52-53 -0.1286891847497742 1213 54-55 -0.029484377500963888 1213 56-57 -0.04613399068337998 1213 58-59 -0.0820147982429944 1213 60-61 -0.03473129838256028 1213 62-63 0.008069548181431685 1213 64-65 0.07310791548731999 1213 66-67 0.08673481527919336 1213 68-69 0.10778199069160621 1213 70-71 0.08844315479480258 1213 72-73 0.10275568460905049 1213 74-75 0.17875294094681493 1213 76-77 0.5610434136310367 1213 78-79 0.11183636383513829 1213 80-81 -0.10985428315743917 1213 82-83 -0.020109156374680026 1213 84-85 0.028601076088556 1213 86-87 0.043827486503161595 1213 88-89 0.07613730599536694 1213 90-91 0.06760407885310826 1213 92-93 0.17342359355327375 1213 94-95 0.27439279894968394 1213 96-97 0.21997953053188013 1213 98-99 0.24404651938560562 1213 100-101 0.5295060569709698 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 27.0 8 288.0 9 842.0 10 580.0 11 205.0 12 220.0 13 472.0 14 1249.0 15 3009.0 16 5792.0 17 9126.0 18 12819.0 19 16333.0 20 20397.0 21 25392.0 22 31237.0 23 38500.0 24 47087.0 25 58364.0 26 72785.0 27 90528.0 28 113806.0 29 143470.0 30 182826.0 31 238600.0 32 319855.0 33 456561.0 34 718513.0 35 1380944.0 36 3144882.0 37 4312725.0 38 733937.0 39 149.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.525885717169945 22.01019641976078 31.146767579732177 27.317150283337092 2 26.574803472801424 21.931721164518056 32.25576939495235 19.237705967728168 3 20.800097196408988 20.242334289973666 39.68755130722602 19.270017206391323 4 20.721564021968153 19.95977604194937 38.81989436996173 20.498765566120753 5 29.5294675869678 19.3511893425451 32.437914151928496 18.681428918558606 6 29.207816430133516 19.74413702066737 32.840409078669985 18.20763747052913 7 29.922719003868153 25.514229751295403 25.966480373549444 18.596570871287 8 27.00459384378961 21.20374961416966 32.1816735514123 19.609982990628428 9 22.245368394092036 20.3436352770426 36.730531165240464 20.680465163624902 10-11 28.21094338755252 21.173081627593497 29.502204997446928 21.113769987407053 12-13 26.845701521649186 26.231455516224578 25.007544214515104 21.915298747611136 14-15 26.851080225472124 23.14444371063357 26.222924892879234 23.78155117101507 16-17 26.412537187477426 22.906402485075755 26.19234709625728 24.48871323118954 18-19 25.989909305242698 23.750771660679458 26.743095278750108 23.516223755327744 20-21 24.461581510686027 24.83299802476277 27.311397189827808 23.394023274723395 22-23 24.53509496351851 24.673497231872542 27.058733228693953 23.73267457591499 24-25 24.72157415494946 25.000098509873975 26.604717637864567 23.673609697311996 26-27 24.563133336398085 24.83184364512803 26.544343398853343 24.06067961962054 28-29 24.741657855505718 24.976308375309486 26.226296061575237 24.05573770760956 30-31 24.86851798553119 24.945856483849216 25.943421318565164 24.242204212054432 32-33 24.82304342971977 24.97185490809029 25.831899467389945 24.373202194799994 34-35 24.681816793722682 25.199133867213767 25.989881873371303 24.12916746569225 36-37 24.86003864470575 25.192333859062778 25.899878812008797 24.04774868422268 38-39 25.01728911375923 24.966851328810066 25.79200396704447 24.22385559038624 40-41 24.605228482364154 25.21049472972984 25.911117605961614 24.27315918194439 42-43 24.66820417473378 25.353720645973393 25.736455815278042 24.24161936401478 44-45 24.6771922296522 24.927804651032588 26.1803742020749 24.214628917240315 46-47 24.39172218331682 25.196618690084943 25.96224584567658 24.449413280921657 48-49 24.746378844552506 24.90722103273688 25.866979249814403 24.479420872896213 50-51 24.611113852085715 25.335316740868635 26.22697584750775 23.826593559537894 52-53 24.954790380455435 25.145633702587478 25.98365946647702 23.915916450480065 54-55 24.47831776224268 25.087741078079933 25.84468517528223 24.589255984395166 56-57 24.76859228420072 25.108834355092757 25.988019866277785 24.134553494428733 58-59 24.488958230803867 24.957435000680622 26.085767298196856 24.467839470318655 60-61 24.721939908321342 25.026974393630862 25.812730485176182 24.438355212871617 62-63 24.23866908213403 25.338891617360908 25.94735296369185 24.475086336813213 64-65 24.61606166739508 25.19208256351506 25.772943108998142 24.41891266009172 66-67 24.58857821608544 25.250700383889807 25.755526869925948 24.405194530098804 68-69 24.501065769271257 25.397698877768526 25.922105150098645 24.17913020286157 70-71 24.402957843225835 25.54347288410404 25.685525155185456 24.368044117484665 72-73 24.709278445319477 25.22321519292385 25.60603930176988 24.461467059986795 74-75 24.76550365543853 25.132595135572984 25.645409901235915 24.45649130775257 76-77 24.70472275268197 24.971209837158323 25.71083681846551 24.613230591694197 78-79 25.01759284987502 24.89508430138991 25.692896388366382 24.394426460368685 80-81 24.846084177210408 25.07506146617054 25.584154387389084 24.49469996922997 82-83 24.638087341909685 25.541628867914916 25.623639896551175 24.196643893624227 84-85 24.388216629948545 25.67242646396222 25.688178929949807 24.251177976139427 86-87 24.403102884323182 25.672960235238012 25.66262801516 24.26130886527881 88-89 24.411704590242174 26.273386115952057 25.20688394905187 24.1080253447539 90-91 24.078842712044285 25.875026892458052 25.531205866647984 24.51492452884968 92-93 24.10971170514351 26.33400456313632 25.154586651672467 24.401697080047704 94-95 24.186934972617063 26.176742031160043 25.136212590756045 24.50011040546685 96-97 24.0111466084141 26.06897790225487 25.083490146579962 24.836385342751065 98-99 24.295265798327854 25.901889955744352 25.08488245629537 24.717961789632426 100-101 24.734887144632577 26.48324887842052 24.45199421738779 24.329869759559116 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 22318.0 1 13190.5 2 6558.5 3 7999.5 4 8252.0 5 6801.5 6 3746.0 7 3415.5 8 3532.0 9 3763.5 10 3801.0 11 3642.0 12 3318.0 13 3113.5 14 3185.5 15 3335.0 16 3553.5 17 3845.0 18 4155.5 19 4522.5 20 5111.5 21 5649.5 22 6166.5 23 7185.5 24 8438.5 25 9884.5 26 11526.5 27 13188.0 28 15537.0 29 18517.0 30 22101.5 31 26906.0 32 35600.0 33 48965.5 34 64381.5 35 89887.5 36 120516.0 37 163058.5 38 222722.5 39 258419.5 40 275392.5 41 284097.0 42 299444.5 43 343928.5 44 404945.5 45 470768.5 46 532393.0 47 573838.0 48 611026.5 49 659186.5 50 703541.0 51 741728.5 52 738811.5 53 693717.0 54 648446.0 55 601982.5 56 538668.0 57 449421.5 58 347611.5 59 268713.0 60 200365.0 61 143180.5 62 107765.0 63 76511.0 64 50600.0 65 34805.0 66 25141.5 67 18444.0 68 14888.0 69 11765.0 70 8920.5 71 7291.5 72 6547.0 73 5668.0 74 4329.0 75 3356.5 76 2718.0 77 2232.0 78 2031.0 79 1941.5 80 1509.5 81 1043.5 82 802.5 83 621.5 84 433.5 85 307.5 86 274.0 87 261.5 88 256.5 89 245.0 90 220.5 91 174.5 92 145.0 93 110.0 94 74.0 95 68.5 96 64.0 97 52.5 98 28.0 99 8.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13838995461978473 2 0.0 3 0.0 4 0.18142235123367198 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.209156164419546E-4 12-13 0.0 14-15 4.8023563561854345E-4 16-17 0.0 18-19 0.0 20-21 0.0037064340082354256 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 4.925493698651728E-5 32-33 0.0 34-35 1.3134649863071274E-4 36-37 2.6679757534363524E-4 38-39 0.009584189821959821 40-41 0.007634515232910178 42-43 9.892033178125553E-4 44-45 0.0013175695643893373 46-47 0.00217132180548897 48-49 1.1903276438408342E-4 50-51 0.0010425628328812823 52-53 0.019862053339813095 54-55 0.018216117528846974 56-57 0.022090839238453 58-59 0.04221558557552752 60-61 0.015708220320616802 62-63 0.002483269739736913 64-65 0.007269207783593508 66-67 0.013073081191838129 68-69 0.02842009864122047 70-71 0.012285002200053852 72-73 0.018860536287753908 74-75 0.013019721676769402 76-77 0.023650578909692715 78-79 0.00940358838634259 80-81 0.006862854553454741 82-83 0.001900419652063125 84-85 0.02031355692885617 86-87 0.009649863071275177 88-89 0.012835015663069962 90-91 0.04686196796458898 92-93 0.035865803282349 94-95 0.03021790384122835 96-97 0.02697939173436484 98-99 0.03359186702480479 100-101 0.24276527067229708 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.218152E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.992418557408065 #Duplication Level Percentage of deduplicated Percentage of total 1 75.93011332597447 20.49537400006131 2 11.415001229906684 6.162369820619381 3 4.164085003999558 3.371961760097469 4 1.9861548144619676 2.1444448828707436 5 1.3403229201868607 1.8089278631885597 6 0.8523226568290005 1.3803749939454277 7 0.4974492207515298 0.9399150304307243 8 0.3386614583494707 0.7313033466424906 9 0.33937861162248406 0.824458458091151 >10 2.20423588955013 12.043382350305645 >50 0.38304793885473704 7.426978911704442 >100 0.48455524888140183 27.18563586687551 >500 0.05109332819840728 9.355716454633107 >1k 0.013242590176702854 5.266623728498883 >5k 2.750987054181712E-4 0.5776072701980977 >10k+ 6.066355109414364E-5 0.2849252618370819 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT 19340 0.15876508021987404 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15461 0.12692176345809061 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.030071780861502E-5 0.0 0.0 2 9.030071780861502E-5 0.0 0.0 3 9.030071780861502E-5 0.0 0.0 4 9.030071780861502E-5 0.0 0.0 5 9.850987397303457E-5 0.0 8.209156164419546E-6 6 1.0671903013745412E-4 0.0 8.209156164419546E-6 7 1.231373424662932E-4 0.0 8.209156164419546E-6 8 1.395556547951323E-4 0.0 8.209156164419546E-6 9 1.6418312328839093E-4 0.0 1.6418312328839093E-5 10-11 2.3396095068595708E-4 0.0 1.6418312328839093E-5 12-13 3.529937150700405E-4 0.0 1.6418312328839093E-5 14-15 5.664317753449487E-4 0.0 1.6418312328839093E-5 16-17 8.742751315106818E-4 0.0 1.6418312328839093E-5 18-19 0.001157491019183156 0.0 1.6418312328839093E-5 20-21 0.0014694389534310988 0.0 1.6418312328839093E-5 22-23 0.0018881059178164957 0.0 1.6418312328839093E-5 24-25 0.0024545376931614446 0.0 1.6418312328839093E-5 26-27 0.0031441068109726864 0.0 1.6418312328839093E-5 28-29 0.0038213621945372986 0.0 1.6418312328839093E-5 30-31 0.004576604561663897 0.0 1.6418312328839093E-5 32-33 0.0054016247561880625 0.0 1.6418312328839093E-5 34-35 0.006407246386329456 0.0 2.0522890411048866E-5 36-37 0.007577051139759242 0.0 2.4627468493258642E-5 38-39 0.008783797095928915 0.0 2.4627468493258642E-5 40-41 0.01015472617538698 0.0 2.4627468493258642E-5 42-43 0.011923799328819392 0.0 2.4627468493258642E-5 44-45 0.0141115394466372 0.0 2.4627468493258642E-5 46-47 0.016381371126099206 0.0 2.4627468493258642E-5 48-49 0.01917248422200185 0.0 2.4627468493258642E-5 50-51 0.022714735106948887 0.0 2.4627468493258642E-5 52-53 0.026958868843953793 0.0 2.8732046575468412E-5 54-55 0.0320444410878117 0.0 4.104578082209773E-5 56-57 0.03861587059742955 0.0 4.104578082209773E-5 58-59 0.04655412460842325 0.0 4.104578082209773E-5 60-61 0.056470785255042066 0.0 4.104578082209773E-5 62-63 0.069129304060577 0.0 5.335951506872706E-5 64-65 0.08416437357571141 0.0 5.746409315093683E-5 66-67 0.10151442512921212 0.0 5.746409315093683E-5 68-69 0.12328921185533497 0.0 5.746409315093683E-5 70-71 0.14958313904997078 0.0 5.746409315093683E-5 72-73 0.1809256972857246 0.0 5.746409315093683E-5 74-75 0.2203173331406918 0.0 5.746409315093683E-5 76-77 0.267868870223092 0.0 5.746409315093683E-5 78-79 0.325550506012386 0.0 6.15686712331466E-5 80-81 0.3947742153688538 0.0 6.977782739756614E-5 82-83 0.47978002745141823 0.0 7.388240547977592E-5 84-85 0.5819019301367974 0.0 7.388240547977592E-5 86-87 0.7054948807702159 0.0 7.388240547977592E-5 88-89 0.8530831948722327 0.0 7.388240547977592E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 153730 0.0 63.16034 1 TTTGTGT 167340 0.0 58.08328 2 TTGTGTT 181350 0.0 54.9391 3 TGTGTTT 194690 0.0 51.36774 4 TAACTTT 25155 0.0 50.17807 1 ACTTTGT 46040 0.0 48.852077 1 AACTTTG 26250 0.0 48.601334 2 GTGTTTG 209380 0.0 47.771214 5 TTTGAGG 61525 0.0 35.785316 8 TTGAGGG 34045 0.0 35.52195 9 TGTTTGA 284715 0.0 35.129383 6 GTTTGAG 162515 0.0 35.04793 7 GTTTGAC 55925 0.0 33.094456 7 TTTGACC 20375 0.0 30.667448 8 TTGAGGC 12640 0.0 29.976229 9 GTTTGAT 69305 0.0 29.103106 7 TTTGATG 19700 0.0 28.536776 8 TTGAGTC 22070 0.0 27.47323 9 GTTTGAA 16790 0.0 27.317808 7 TTGACCC 12450 0.0 27.153875 9 >>END_MODULE