FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l05n01_30112.34100000035b54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l05n01_30112.34100000035b54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12181520
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT193400.15876508021987404No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154610.12692176345809061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1537300.063.160341
TTTGTGT1673400.058.083282
TTGTGTT1813500.054.93913
TGTGTTT1946900.051.367744
TAACTTT251550.050.178071
ACTTTGT460400.048.8520771
AACTTTG262500.048.6013342
GTGTTTG2093800.047.7712145
TTTGAGG615250.035.7853168
TTGAGGG340450.035.521959
TGTTTGA2847150.035.1293836
GTTTGAG1625150.035.047937
GTTTGAC559250.033.0944567
TTTGACC203750.030.6674488
TTGAGGC126400.029.9762299
GTTTGAT693050.029.1031067
TTTGATG197000.028.5367768
TTGAGTC220700.027.473239
GTTTGAA167900.027.3178087
TTGACCC124500.027.1538759
Produced by FastQC (version 0.11.2)