FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l04n02_8033.34200000035d6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l04n02_8033.34200000035d6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17579714
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTCG11700.0101.0618394-95
ACATCCG16350.089.3358294-95
AGATCGA9100.076.4333294-95
CTTTGTG1353450.064.5231861
TATATCG8201.36406015E-859.37563794-95
TTTGTGT1478300.059.3099942
ACTTTGT387050.057.353011
TTGTGTT1642400.054.7667273
TGTGTTT1719400.052.5180554
TATACTG9604.728645E-850.71668694-95
CTATACG5704.711771E-448.81004394-95
ACATCCA39900.047.0668394-95
GTGTTTG1918150.047.0602265
TTCGACA13603.074092E-1046.0285994-95
CATGTAA13851.8551873E-840.17577794-95
CTTATAG10655.6062618E-639.1855394-95
TATGCTA16051.5643309E-939.0024294-95
TATATTG20451.0913936E-1137.41308294-95
CGGAAGG21001.4551915E-1136.43321294-95
CGTCGTA15856.086884E-835.10627794-95
Produced by FastQC (version 0.11.2)