FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l04n02_7965.34200000035ca0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l04n02_7965.34200000035ca0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14696702
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA4000.0192.1308694-95
GAACCTA4400.0158.7858394-95
GAGCGTA6800.0113.0181594-95
CACGATA3804.3601176E-9110.3143894-95
ACGTGTA5700.0110.3143794-95
CGCTAAA6500.0107.48579494-95
TGCACGG3302.424822E-7105.8572294-95
CGCCTGA4751.7462298E-10102.96007594-95
TGCCCGA4107.394192E-9102.24258494-95
CCGCCTA4208.742063E-999.80823594-95
TGGCTGA13300.099.80823594-95
CGAGGTA2206.5429846E-495.271594-95
ACCTAGG3052.0864427E-591.62723594-95
ACGTATA2307.8072824E-491.12926594-95
ATGTACG3102.261815E-590.14937694-95
TACGTAA4651.7724233E-890.14937694-95
ACGCCTA3906.558312E-789.571594-95
GGATGTA6301.6370905E-1188.7184494-95
ACGCCGA7200.087.33221494-95
TGACGTA5707.403287E-1085.80006494-95
Produced by FastQC (version 0.11.2)