FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l04n02_7765.34200000035c6c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l04n02_7765.34200000035c6c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14425059
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC159550.11060613339605752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTCG4800.0142.8589694-95
GCTATAA7700.0106.8659394-95
AGATCGA5859.094947E-1293.77408694-95
TATTATA9400.087.5391194-95
ACATCCG6904.3655746E-1179.5041294-95
CTCGCAA4652.0862171E-673.7336694-95
CTATATG14450.071.1823294-95
TATGCTA9650.071.0593894-95
GTAAATA8855.456968E-1269.7345594-95
GCGTAAG5901.05143044E-769.7345494-95
GTGCTCA4151.05349325E-466.0937894-95
CGCTATG4351.3297924E-463.0549994-95
CTTTGTG1433300.059.029481
TTTGTGT1514100.056.0945662
ATATTAG12501.8189894E-1254.8578494-95
TATATTG15050.054.6755994-95
ACTTTGT484300.054.4753571
AGAGCGA16400.054.35609494-95
CGGAAGG14100.053.49612494-95
TTGTGTT1664650.052.318233
Produced by FastQC (version 0.11.2)