##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l04n02_7331.34200000035c1c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19885260 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.345466692414384 33.0 33.0 33.0 33.0 33.0 2 32.35675686412951 33.0 33.0 33.0 33.0 33.0 3 32.37335508814066 33.0 33.0 33.0 33.0 33.0 4 36.22416397874606 37.0 37.0 37.0 37.0 37.0 5 36.2191364860203 37.0 37.0 37.0 37.0 37.0 6 36.210122925221995 37.0 37.0 37.0 37.0 37.0 7 36.19185341303056 37.0 37.0 37.0 37.0 37.0 8 36.21570349092745 37.0 37.0 37.0 37.0 37.0 9 36.154170878328976 37.0 37.0 37.0 37.0 37.0 10-11 36.158526843501164 37.0 37.0 37.0 37.0 37.0 12-13 36.12960423952214 37.0 37.0 37.0 37.0 37.0 14-15 38.098946506105534 40.0 37.0 40.0 37.0 40.0 16-17 38.09393171122731 40.0 37.0 40.0 37.0 40.0 18-19 37.96955297541999 40.0 37.0 40.0 37.0 40.0 20-21 37.95433373765292 40.0 37.0 40.0 37.0 40.0 22-23 37.90254784196938 40.0 37.0 40.0 35.0 40.0 24-25 37.8628763214562 40.0 37.0 40.0 33.0 40.0 26-27 37.78820603804024 40.0 37.0 40.0 33.0 40.0 28-29 37.70810736193542 40.0 37.0 40.0 33.0 40.0 30-31 37.64924934348357 40.0 37.0 40.0 33.0 40.0 32-33 37.60265877841175 40.0 37.0 40.0 33.0 40.0 34-35 37.46405910709741 40.0 37.0 40.0 33.0 40.0 36-37 37.41298542236812 40.0 37.0 40.0 33.0 40.0 38-39 37.28829215710532 40.0 37.0 40.0 33.0 40.0 40-41 37.19639049225406 40.0 37.0 40.0 33.0 40.0 42-43 37.065913872888764 40.0 37.0 40.0 33.0 40.0 44-45 36.85541986878723 38.5 37.0 40.0 33.0 40.0 46-47 36.72307329650204 37.0 37.0 40.0 33.0 40.0 48-49 36.55417736554614 37.0 37.0 40.0 33.0 40.0 50-51 35.22252831997167 37.0 35.0 38.5 30.0 38.5 52-53 35.583119355743904 37.0 35.0 38.5 33.0 40.0 54-55 36.20112216284826 37.0 37.0 40.0 33.0 40.0 56-57 36.08273193310019 37.0 37.0 40.0 33.0 40.0 58-59 35.857158065823626 37.0 37.0 40.0 33.0 40.0 60-61 35.61135343465462 37.0 37.0 37.0 33.0 40.0 62-63 35.338168774257916 37.0 33.0 37.0 33.0 40.0 64-65 35.09943878028248 37.0 33.0 37.0 33.0 40.0 66-67 34.8478427237059 37.0 33.0 37.0 30.0 38.5 68-69 34.60410972750671 37.0 33.0 37.0 27.0 37.0 70-71 34.40149796884728 37.0 33.0 37.0 27.0 37.0 72-73 34.206799835657165 37.0 33.0 37.0 27.0 37.0 74-75 33.99451495731009 37.0 33.0 37.0 27.0 37.0 76-77 33.89860620379115 37.0 33.0 37.0 27.0 37.0 78-79 33.778383486059525 37.0 33.0 37.0 27.0 37.0 80-81 33.64173862951755 37.0 33.0 37.0 27.0 37.0 82-83 33.50019723151721 37.0 33.0 37.0 27.0 37.0 84-85 33.39329523476183 37.0 33.0 37.0 27.0 37.0 86-87 33.31906004749247 37.0 33.0 37.0 27.0 37.0 88-89 33.224044266959545 37.0 33.0 37.0 27.0 37.0 90-91 33.08558062605166 37.0 33.0 37.0 27.0 37.0 92-93 33.00590482598669 35.0 33.0 37.0 27.0 37.0 94-95 32.871231882308805 33.0 33.0 37.0 27.0 37.0 96-97 32.7677295645116 33.0 33.0 37.0 27.0 37.0 98-99 31.362181937777027 33.0 30.0 37.0 21.0 37.0 100-101 2.1707391555353057 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2541149139712573 2306 2 0.20889445906168191 2306 3 0.20304708103876123 2306 4 0.2273859192611667 2306 5 0.14474852373255231 2306 6 0.23005787929795218 2306 7 0.13969167675593042 2306 8 0.17569005338285137 2306 9 0.24607838181348995 2306 10-11 0.24597839364830776 2306 12-13 0.19243156314218623 2306 14-15 0.26937915709532234 2306 16-17 0.2766021833753527 2306 18-19 0.38540791877270664 2306 20-21 0.30341226928510423 2306 22-23 0.27995346019219625 2306 24-25 0.31155025551036175 2306 26-27 0.2693178544258572 2306 28-29 0.2123046806596136 2306 30-31 0.29433671595612054 2306 32-33 0.31208396526562865 2306 34-35 0.3638658300836042 2306 36-37 0.22198309012780726 2306 38-39 0.3004399141338965 2306 40-41 0.18183722936182534 2306 42-43 0.25387235630322635 2306 44-45 0.2999370179229146 2306 46-47 0.20686886912663738 2306 48-49 0.23209914117921926 2306 50-51 0.05687246537671342 2306 52-53 0.1935203032744468 2306 54-55 0.31065614892117566 2306 56-57 0.2992780963944597 2306 58-59 0.2321189124109182 2306 60-61 0.20373134801194936 2306 62-63 0.23240146641993675 2306 64-65 0.23143293856610825 2306 66-67 0.2659211448790586 2306 68-69 0.21524771029965706 2306 70-71 0.23071192091728676 2306 72-73 0.21220613279300693 2306 74-75 0.08514832326735444 2306 76-77 0.18509280843261422 2306 78-79 0.15353417412551806 2306 80-81 0.13978619360867128 2306 82-83 0.028694876722582308 2306 84-85 0.030517757813342428 2306 86-87 0.012769152702261977 2306 88-89 0.010736570191127726 2306 90-91 -0.022063264735081134 2306 92-93 -0.14236137040101227 2306 94-95 -0.10705918771896705 2306 96-97 -0.05670277922603617 2306 98-99 1.0506045105000048 2306 100-101 2.3312926238048957 2307 1 0.21317080808402977 2307 2 0.20141524138747968 2307 3 0.16165377548960436 2307 4 0.1457568470583368 2307 5 0.1458347510321758 2307 6 0.1676138317135596 2307 7 0.16579432795445825 2307 8 0.16779635882075894 2307 9 0.22066090049941067 2307 10-11 0.18227605844576544 2307 12-13 0.17537559188753704 2307 14-15 0.2537774174030929 2307 16-17 0.18171832833136392 2307 18-19 0.21129199238533403 2307 20-21 0.2617344718071877 2307 22-23 0.25599891568731437 2307 24-25 0.1632084130587259 2307 26-27 0.13370350685776344 2307 28-29 0.24062054486919493 2307 30-31 0.1392427983968645 2307 32-33 0.0764594437193864 2307 34-35 0.18911572423673562 2307 36-37 0.14169930444009537 2307 38-39 0.15947325049695138 2307 40-41 0.12926595839044097 2307 42-43 0.07866098398312005 2307 44-45 0.12858084672437542 2307 46-47 0.21015872473739705 2307 48-49 0.17966456043907897 2307 50-51 0.07691227826479974 2307 52-53 0.12871391064121696 2307 54-55 0.29875085789897327 2307 56-57 0.38524533680980255 2307 58-59 0.2767447707259265 2307 60-61 0.26733648355397577 2307 62-63 0.25141181914582234 2307 64-65 0.23577639242589044 2307 66-67 0.28394672437617174 2307 68-69 0.32839390969605375 2307 70-71 0.3065519816950868 2307 72-73 0.2615085568158406 2307 74-75 0.10219655354871549 2307 76-77 0.10863965274941023 2307 78-79 0.14722027649815317 2307 80-81 0.12198651749741884 2307 82-83 0.11486399404270031 2307 84-85 0.13077745987783373 2307 86-87 0.05103793090167841 2307 88-89 0.04224124218546166 2307 90-91 0.07653118612098808 2307 92-93 -0.029867734159616077 2307 94-95 -0.15145686589125518 2307 96-97 -0.06255541930863728 2307 98-99 1.1098995801518008 2307 100-101 2.328644764518826 2201 1 0.252071224206901 2201 2 0.24308151511959863 2201 3 0.197057683467726 2201 4 0.217210273513345 2201 5 0.21790422961461076 2201 6 0.2257538602914977 2201 7 0.25041311116738285 2201 8 0.22359884575369904 2201 9 0.24799763723444812 2201 10-11 0.21403991426987545 2201 12-13 0.20256537802659835 2201 14-15 0.24352798463093706 2201 16-17 0.2866773838593488 2201 18-19 0.40123754515907706 2201 20-21 0.3219267819361846 2201 22-23 0.250011797985799 2201 24-25 0.27946226650030326 2201 26-27 0.3015771611261826 2201 28-29 0.2699853846983373 2201 30-31 0.1956010886476065 2201 32-33 0.27329526472651366 2201 34-35 0.33927079663397564 2201 36-37 0.240166433890181 2201 38-39 0.24288553078508812 2201 40-41 0.21966826381877524 2201 42-43 0.2797670653012574 2201 44-45 0.34649859967331054 2201 46-47 0.2084627846202025 2201 48-49 0.22699396262673588 2201 50-51 0.15785127273595378 2201 52-53 0.2567114836558986 2201 54-55 0.3407816987255856 2201 56-57 0.4067500074686876 2201 58-59 0.2746660777418555 2201 60-61 0.26322161760420215 2201 62-63 0.31990922151958756 2201 64-65 0.34437254011638174 2201 66-67 0.324556312297581 2201 68-69 0.3656310494676873 2201 70-71 0.30543176511925907 2201 72-73 0.20745860296054985 2201 74-75 0.014085291933866984 2201 76-77 0.04267848534537677 2201 78-79 0.08879706501330276 2201 80-81 0.04843837256044736 2201 82-83 -0.032014346982805364 2201 84-85 -0.07190018998445424 2201 86-87 -0.04856603262042114 2201 88-89 -0.04793226109223525 2201 90-91 -0.17319528989175836 2201 92-93 -0.1930817697954197 2201 94-95 -0.28444070558393264 2201 96-97 -0.32630965858770367 2201 98-99 0.9660192223854231 2201 100-101 2.3258279015822194 1101 1 0.14849227639366802 1101 2 0.13956618416690247 1101 3 0.1481021659055699 1101 4 0.1674234917985089 1101 5 0.20610159247292614 1101 6 0.23488818118438815 1101 7 0.1934784796044724 1101 8 0.18665840569482128 1101 9 0.20124861823448015 1101 10-11 0.1867312628186255 1101 12-13 0.21372181999244333 1101 14-15 0.28337479515391806 1101 16-17 0.2963460902224071 1101 18-19 0.3317308332153388 1101 20-21 0.2385181820968043 1101 22-23 0.27078662138274723 1101 24-25 0.27504746382494005 1101 26-27 0.19349669616991605 1101 28-29 0.23036638092838757 1101 30-31 0.3041601494691264 1101 32-33 0.2934204835295233 1101 34-35 0.3739848290497605 1101 36-37 0.291386851905969 1101 38-39 0.24465532610667395 1101 40-41 0.15939345895753831 1101 42-43 0.21036206926482492 1101 44-45 0.29129045902011796 1101 46-47 0.25661368911481475 1101 48-49 0.21340375316937354 1101 50-51 0.2677172835650339 1101 52-53 0.33594070354069316 1101 54-55 0.39933862746029547 1101 56-57 0.3431398313064449 1101 58-59 0.3177467357384245 1101 60-61 0.29652008843697075 1101 62-63 0.2917252523922116 1101 64-65 0.3255685332529836 1101 66-67 0.28410166495338274 1101 68-69 0.27748669105582735 1101 70-71 0.31826373919923157 1101 72-73 0.3360351737851133 1101 74-75 0.08990045498952526 1101 76-77 0.033005149856904836 1101 78-79 0.08242158762273988 1101 80-81 0.10133698299687666 1101 82-83 0.12528308508190378 1101 84-85 0.057372363124187586 1101 86-87 0.08531201047731685 1101 88-89 -0.01701863500225187 1101 90-91 -0.07007628698616486 1101 92-93 -0.22014136085348213 1101 94-95 -0.12890480940541948 1101 96-97 -0.0841365108573271 1101 98-99 1.0353217149869813 1101 100-101 2.3145545405855406 2304 1 0.19151825116304622 2304 2 0.14394170294729491 2304 3 0.12220367948660282 2304 4 0.1120568075063062 2304 5 0.12791581653878836 2304 6 0.10602861959525711 2304 7 0.06825695228528161 2304 8 0.11315120721116756 2304 9 0.16284432533139892 2304 10-11 0.15024443105782836 2304 12-13 0.15630835933639986 2304 14-15 0.2263602120960897 2304 16-17 0.2350052210013729 2304 18-19 0.28146450088895136 2304 20-21 0.23386479408540595 2304 22-23 0.21780262806841932 2304 24-25 0.21025407418471787 2304 26-27 0.18862349492405173 2304 28-29 0.26284603992564826 2304 30-31 0.26818439798360316 2304 32-33 0.2669015196673854 2304 34-35 0.3329366710168742 2304 36-37 0.22686870287972738 2304 38-39 0.2206929487981668 2304 40-41 0.24961418990836393 2304 42-43 0.2152358719439249 2304 44-45 0.27799226070889915 2304 46-47 0.22992317172868582 2304 48-49 0.18689323059959406 2304 50-51 0.11169546866170066 2304 52-53 0.2179229489485195 2304 54-55 0.2760401829152386 2304 56-57 0.3049890283612342 2304 58-59 0.2475239004788392 2304 60-61 0.2893537814627578 2304 62-63 0.29788081189600035 2304 64-65 0.3314491988513666 2304 66-67 0.2867601519865133 2304 68-69 0.3155766891008156 2304 70-71 0.3007852367227031 2304 72-73 0.28774139742332494 2304 74-75 0.060480499137561594 2304 76-77 0.05707604694835311 2304 78-79 0.056797659014137025 2304 80-81 0.005348624055578455 2304 82-83 0.00694504960200959 2304 84-85 -0.03193061962380028 2304 86-87 -0.014806875184355306 2304 88-89 0.0015253665962902119 2304 90-91 -0.024882085255413244 2304 92-93 -0.06476161130868263 2304 94-95 -0.0573163350738497 2304 96-97 -0.09567487660189755 2304 98-99 1.1086679274823972 2304 100-101 2.3297128834515903 2202 1 0.18186031947636394 2202 2 0.1182311569322465 2202 3 0.0995608170033151 2202 4 0.1300489821727311 2202 5 0.1269615577542993 2202 6 0.17555231191565923 2202 7 0.1681929905502102 2202 8 0.14232792708985187 2202 9 0.15371458272353067 2202 10-11 0.13444066348272798 2202 12-13 0.13915906433197023 2202 14-15 0.18943474489341128 2202 16-17 0.18552183473816086 2202 18-19 0.30092238149038764 2202 20-21 0.21636795375370355 2202 22-23 0.15478083128030562 2202 24-25 0.17049408826425605 2202 26-27 0.16696332743714493 2202 28-29 0.1554118704688463 2202 30-31 0.1573161230166562 2202 32-33 0.18760808402296902 2202 34-35 0.27263437021425574 2202 36-37 0.2261199328749086 2202 38-39 0.2774865238139341 2202 40-41 0.21611235447597466 2202 42-43 0.20064593430843303 2202 44-45 0.3050462338350357 2202 46-47 0.2563280301992563 2202 48-49 0.16138097133693918 2202 50-51 0.1097317792386221 2202 52-53 0.2034486172850194 2202 54-55 0.2715496337149119 2202 56-57 0.24964577879093497 2202 58-59 0.30791101842300606 2202 60-61 0.3485523961596897 2202 62-63 0.27618775198377676 2202 64-65 0.27581394442847795 2202 66-67 0.2657060082267293 2202 68-69 0.328552853280506 2202 70-71 0.2969905858656148 2202 72-73 0.2887631105001489 2202 74-75 0.04133295939627857 2202 76-77 -0.0033826243491787977 2202 78-79 -0.006320865456537206 2202 80-81 0.026665017613481723 2202 82-83 -0.09422390331948804 2202 84-85 -0.024016553335151514 2202 86-87 -0.04570887542980273 2202 88-89 -0.0447707048262771 2202 90-91 -0.08353605073520498 2202 92-93 -0.02753485329103711 2202 94-95 -0.020216611904970705 2202 96-97 -0.012134384501067075 2202 98-99 1.1284403774317973 2202 100-101 2.3421131836221263 1102 1 0.023382209440882207 1102 2 0.03515409569144623 1102 3 -0.022549358700864275 1102 4 0.010782313495433016 1102 5 -0.04273335542404055 1102 6 -0.02862513124617294 1102 7 -0.01699880426642153 1102 8 0.00668993433595233 1102 9 0.020056152564173146 1102 10-11 -0.04077345755730022 1102 12-13 0.06631935498673869 1102 14-15 0.040955412077785525 1102 16-17 0.01681443620777401 1102 18-19 0.09264630677525787 1102 20-21 0.001144969600389345 1102 22-23 0.05226096667136204 1102 24-25 0.11519401848474331 1102 26-27 0.04824154884772014 1102 28-29 0.10706821098936814 1102 30-31 0.023323679837844224 1102 32-33 0.14353786802073643 1102 34-35 0.22368325628382735 1102 36-37 0.10285241026699055 1102 38-39 0.12565958449253145 1102 40-41 0.11249071369943664 1102 42-43 0.14363366668788302 1102 44-45 0.10570496086189962 1102 46-47 0.12226477163018501 1102 48-49 0.05822699418114752 1102 50-51 -0.06964561674059411 1102 52-53 0.07654378829729325 1102 54-55 0.16534709796439273 1102 56-57 0.24175609606234616 1102 58-59 0.17365218039871877 1102 60-61 0.19810091199675384 1102 62-63 0.18212335785063516 1102 64-65 0.19014717733320907 1102 66-67 0.1656656733686006 1102 68-69 0.2143627618523496 1102 70-71 0.25790432014022713 1102 72-73 0.2218791432806384 1102 74-75 -0.04311004403620444 1102 76-77 -0.09736456570895058 1102 78-79 -0.004406068810354213 1102 80-81 -0.062752439773341 1102 82-83 -0.08313131951945252 1102 84-85 -0.05015359526749563 1102 86-87 -0.058723328185031676 1102 88-89 -0.08366164832634837 1102 90-91 -0.05984390784416149 1102 92-93 -0.15942199536297608 1102 94-95 -0.13807427810606043 1102 96-97 -0.09805616477549961 1102 98-99 1.0366074844185498 1102 100-101 2.3450162620665975 2305 1 0.08023583724149574 2305 2 0.07022846574933084 2305 3 0.08945536648376162 2305 4 0.028569305671943823 2305 5 0.06578557727561929 2305 6 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-0.2513749829609786 1213 100-101 -0.21052135498215874 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1014.0 3 274.0 4 334.0 5 611.0 6 2136.0 7 18377.0 8 27604.0 9 24863.0 10 19816.0 11 16105.0 12 15293.0 13 15837.0 14 17709.0 15 20117.0 16 23334.0 17 26971.0 18 31799.0 19 36869.0 20 43605.0 21 50451.0 22 59497.0 23 70610.0 24 84564.0 25 104034.0 26 130258.0 27 166716.0 28 202736.0 29 253646.0 30 333967.0 31 455041.0 32 648094.0 33 994582.0 34 1724530.0 35 3651757.0 36 7355226.0 37 3244948.0 38 11935.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.889065918353257 22.286297295594863 30.719745520312674 26.104891265739205 2 27.971871390501494 22.296948330225547 29.405257820422808 20.325922458850144 3 21.787554468282032 20.451563656047625 36.64678273141494 21.11409914425541 4 21.496711321667213 20.70764590350959 35.81431113371254 21.981331641110646 5 27.97639928519708 19.961054301602008 31.547948091793128 20.51459832140779 6 29.90450159189175 20.644691753333422 29.628943735309864 19.821862919464962 7 28.302160763859025 26.777857172085056 24.47246727392323 20.447514790132697 8 26.61757963673598 22.336437398182397 29.614763736854716 21.431219228226905 9 23.105459698742955 21.21174748033769 33.35686363896755 22.3259291819518 10-11 27.579889565552868 21.990180640676833 27.52470254146199 22.90522725230831 12-13 26.6568076586361 25.423797408408777 24.38251581991751 23.53687911303761 14-15 26.204879148290043 23.714894650920513 25.84268466761709 24.23754153317235 16-17 25.732469220308378 23.50086120296317 25.307818307553532 25.45885126917492 18-19 25.722610177897533 24.051105904588148 25.68083685372624 24.545447063788078 20-21 24.83326348177448 24.385578490947626 26.191551244662172 24.589606782615725 22-23 24.934900686957644 24.52690774122688 25.56488494553122 24.973306626284263 24-25 25.418377896736576 24.40537919742455 25.264392046359113 24.911850859479763 26-27 25.08269370787266 24.494426652889313 25.57912655176168 24.843753087476347 28-29 25.075848962807047 25.05312227644425 24.947843022505815 24.923185738242893 30-31 25.384701543292465 24.80198943696943 24.816107917962864 24.99720110177524 32-33 25.08742647057158 25.08102218187319 24.97937423805894 24.852177109496292 34-35 24.918894837121076 25.277576057455853 24.877981021910454 24.925548083512616 36-37 25.13276687910525 24.96333509222182 24.997158515274577 24.906739513398353 38-39 25.097238735898824 24.640690359962914 25.12282075387538 25.139250150262882 40-41 24.84927254709308 25.07762421031582 24.73215280025116 25.34095044233994 42-43 24.836020153828052 24.947036107368785 24.908930849112465 25.3080128896907 44-45 25.223217230397406 24.749116954721114 24.869133067307814 25.15853274757367 46-47 24.781308642557168 24.85584643573093 25.120716682668903 25.242128239042998 48-49 24.929262718397315 24.58730773938951 24.987420091205788 25.49600945100739 50-51 24.790980313877007 24.881004070894484 25.430448824531517 24.89756679069699 52-53 24.93375182598713 24.663182059084267 25.519286354488845 24.883779760439754 54-55 24.701713136708904 24.83772117390458 25.00042367163331 25.460142017753213 56-57 24.835720685487743 25.052084387583978 25.239019705763493 24.873175221164793 58-59 24.64916361960334 25.24581160280663 25.200813391632792 24.90421138595724 60-61 25.21771320025878 25.051295729239325 24.72276134459596 25.008229725905938 62-63 24.779688740848847 25.258757317960047 25.267660562796333 24.693893378394776 64-65 25.033682820416114 25.108734913214082 25.025353208641217 24.83222905772859 66-67 25.150501391053105 25.214876755562184 25.154365322566413 24.480256530818302 68-69 25.038958018901276 24.847818743719333 25.52330340635039 24.589919831029004 70-71 24.880492019134973 25.350155482415172 25.119529380357065 24.649823118092794 72-73 24.828279502421754 24.94761431213071 25.409983514076934 24.814122671370605 74-75 24.86441050251563 24.869114914626763 25.36522251125953 24.90125207159808 76-77 24.7574578183182 24.935235475127936 25.22737833374689 25.079928372806968 78-79 24.97526964704554 24.81253878295007 25.05299181317443 25.15919975682996 80-81 24.875477903093092 24.682036389976133 25.39511609528634 25.047369611644438 82-83 24.645700246790295 25.478867043392583 25.205384658508617 24.670048051308505 84-85 24.838644052555317 25.146007436441792 25.079784534124556 24.93556397687834 86-87 24.728164113578522 25.24075636535527 25.274540808863783 24.756538712202424 88-89 24.819824603506042 26.26888460150302 24.53801611611971 24.37327467887123 90-91 24.908043365836406 25.27265265793906 24.79150453950183 25.02779943672271 92-93 24.680602345271264 25.583543775773254 24.827646659072364 24.908207219883113 94-95 24.80672239772648 25.751678794093802 24.48001294501546 24.961585863164263 96-97 24.761368538658036 25.208959353999578 24.79580460889603 25.23386749844636 98-99 25.210215161318906 25.063960454520984 24.74003858826709 24.985785795893023 100-101 33.674872376630745 49.93390054830781 6.464662507090186 9.92656456797126 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9149.0 1 5719.5 2 2917.0 3 3158.0 4 2844.0 5 2414.5 6 1943.0 7 2083.0 8 2304.5 9 2433.5 10 2525.0 11 2816.0 12 3156.0 13 3402.5 14 3557.5 15 3601.5 16 3571.5 17 3370.0 18 3179.5 19 3216.5 20 3441.5 21 3690.0 22 4326.5 23 5513.5 24 6667.0 25 8049.0 26 10195.5 27 12640.0 28 15145.5 29 18289.5 30 22848.5 31 29787.5 32 39748.0 33 52157.5 34 68993.0 35 91687.5 36 118701.5 37 149151.0 38 185007.5 39 247683.5 40 327468.5 41 401879.0 42 471762.5 43 566696.5 44 709954.5 45 884717.0 46 1089856.0 47 1260905.5 48 1356987.5 49 1353021.0 50 1270906.0 51 1203374.5 52 1149123.5 53 1081825.5 54 1017485.0 55 983851.0 56 907533.0 57 733839.0 58 546151.5 59 407703.5 60 312191.5 61 232184.5 62 159606.5 63 103397.0 64 64770.0 65 39580.0 66 23798.0 67 16422.0 68 12184.5 69 9130.5 70 7049.5 71 5450.5 72 4135.0 73 3138.5 74 2381.0 75 1771.5 76 1363.0 77 1100.0 78 883.5 79 675.5 80 506.5 81 388.0 82 299.0 83 230.5 84 189.0 85 156.0 86 132.5 87 116.0 88 103.0 89 90.5 90 70.5 91 57.0 92 48.5 93 35.0 94 26.5 95 19.5 96 11.0 97 7.5 98 4.5 99 3.5 100 1.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.04574242428814107 2 0.07950612664858292 3 0.11226405890594339 4 0.06227225593228351 5 0.06783416460232353 6 0.0700720030816796 7 0.08436902509698138 8 0.0855457761175866 9 0.10106983765864766 10-11 0.08732598920004063 12-13 0.09637289127725762 14-15 0.09207322408658473 16-17 0.08755983074900706 18-19 0.09310665286750085 20-21 0.08517364117944648 22-23 0.09978748077721891 24-25 0.1044617973312896 26-27 0.10936492658381132 28-29 0.10534184617148581 30-31 0.12454702628982472 32-33 0.11852749222288268 34-35 0.11453709933890732 36-37 0.11709426982599172 38-39 0.138602663480387 40-41 0.11192209707089572 42-43 0.10333030596532307 44-45 0.1257715513903263 46-47 0.12841672676143032 48-49 0.13182125856036078 50-51 0.12266120734654713 52-53 0.13661123867628586 54-55 0.14624148741329004 56-57 0.1266365136789763 58-59 0.12305848653726427 60-61 0.13763460975617117 62-63 0.10920400336731831 64-65 0.11259344861470255 66-67 0.11099427415080317 68-69 0.13429042416342557 70-71 0.12559805604754476 72-73 0.1286857702640046 74-75 0.21214457341769732 76-77 0.22785218800257076 78-79 0.2378143408735918 80-81 0.2621715783449651 82-83 0.25041664026520144 84-85 0.23234295151282908 86-87 0.21024366792287355 88-89 0.2022729398559536 90-91 0.19655010796942057 92-93 0.20398023460593426 94-95 0.20783484852599363 96-97 0.19279607105966934 98-99 0.19127484377875872 100-101 96.67530120300162 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.988526E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.47856836364531 #Duplication Level Percentage of deduplicated Percentage of total 1 76.53085755978279 17.203041135859788 2 10.233893308547186 4.600865407248604 3 3.9462454033110204 2.661178412341435 4 2.0554227269701504 1.8481184113755518 5 1.0033672100758162 1.1277129212764652 6 0.8449424814938378 1.1395858400164407 7 0.5913415658383635 0.9304758269773903 8 0.41402882644447453 0.7445420223801568 9 0.31634057412541206 0.6399794899505593 >10 2.913050341119746 13.865107368498256 >50 0.5324755870379729 8.328270755384663 >100 0.5173558795608226 23.716426536764644 >500 0.06693096460893445 10.25999133784624 >1k 0.03338802492726502 12.406989330876605 >5k 3.595461562034201E-4 0.5277152032034435 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.6092321649302047E-4 0.0 0.0 2 1.8103861855464802E-4 0.0 0.0 3 1.910963195854618E-4 0.0 0.0 4 1.910963195854618E-4 0.0 0.0 5 2.0115402061627558E-4 0.0 0.0 6 2.0115402061627558E-4 0.0 0.0 7 2.313271237087169E-4 0.0 0.0 8 2.5647137628575134E-4 0.0 0.0 9 2.71557927831972E-4 0.0 0.0 10-11 3.092743066975237E-4 0.0 0.0 12-13 3.721349381401098E-4 2.514425257703445E-6 0.0 14-15 4.953417757675786E-4 7.543275773110335E-6 0.0 16-17 6.260918891681578E-4 1.005770103081378E-5 0.0 18-19 7.74442979372661E-4 1.005770103081378E-5 0.0 20-21 0.0010133133788544882 1.005770103081378E-5 0.0 22-23 0.0012597270541094257 1.005770103081378E-5 0.0 24-25 0.0014508233736948875 1.005770103081378E-5 0.0 26-27 0.0017173524510114527 1.005770103081378E-5 0.0 28-29 0.002024112332451273 1.005770103081378E-5 0.0 30-31 0.002350987615952721 1.005770103081378E-5 0.0 32-33 0.0028362716906894857 1.005770103081378E-5 0.0 34-35 0.003560426164908078 1.005770103081378E-5 0.0 36-37 0.004302181615930593 1.005770103081378E-5 0.0 38-39 0.005413557579835516 1.005770103081378E-5 0.0 40-41 0.006738659690645231 1.005770103081378E-5 0.0 42-43 0.008473613118460607 1.005770103081378E-5 0.0 44-45 0.010676249644208826 1.005770103081378E-5 0.0 46-47 0.013733790757576216 1.005770103081378E-5 0.0 48-49 0.017412394909596354 1.005770103081378E-5 0.0 50-51 0.02207413933737854 1.005770103081378E-5 0.0 52-53 0.027799485649169287 1.005770103081378E-5 0.0 54-55 0.03484239079599663 1.005770103081378E-5 0.0 56-57 0.04361773494538165 1.005770103081378E-5 0.0 58-59 0.054404619300929435 1.005770103081378E-5 0.0 60-61 0.06785930885490056 1.005770103081378E-5 0.0 62-63 0.08475373216141001 1.005770103081378E-5 0.0 64-65 0.105560601168906 1.005770103081378E-5 0.0 66-67 0.13113230603975007 1.5086551546220668E-5 0.0 68-69 0.16251736210640444 1.5086551546220668E-5 0.0 70-71 0.20077685682762006 1.5086551546220668E-5 0.0 72-73 0.24762311380389296 1.5086551546220668E-5 0.0 74-75 0.3047684566357191 1.5086551546220668E-5 0.0 76-77 0.37383217518905965 1.5086551546220668E-5 0.0 78-79 0.45924217234273024 1.5086551546220668E-5 0.0 80-81 0.561710030444661 1.5086551546220668E-5 0.0 82-83 0.6845874783633706 1.5086551546220668E-5 0.0 84-85 0.8325412893771568 1.5086551546220668E-5 0.0 86-87 1.0033788846613019 1.5086551546220668E-5 0.0 88-89 1.1504677333864382 1.5086551546220668E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCGA 1280 0.0 92.812325 94-95 GTTCTCG 1610 0.0 69.448166 94-95 ACATCCG 2130 0.0 68.897964 94-95 CTTTGTG 158020 0.0 68.10597 1 CTAGTTA 2630 0.0 66.42797 94-95 TTTGTGT 168180 0.0 64.18807 2 TTGTGTT 191580 0.0 57.83689 3 AACTTTG 17440 0.0 56.104374 2 TAACTTT 17720 0.0 54.456623 1 ACTTTGT 40505 0.0 54.21428 1 TGTGTTT 206300 0.0 53.95741 4 CTCTCGG 1570 0.0 53.413162 94-95 GTGTTTG 237695 0.0 46.8044 5 CGTCGTA 2845 0.0 44.213707 94-95 CATTCGA 1800 1.8189894E-12 42.705803 94-95 TCTCGCG 1205 2.7222086E-7 40.595486 94-95 GGATGTA 1780 9.458745E-11 39.259674 94-95 GAGCGTA 3360 0.0 37.436905 94-95 CCGCTAA 2650 0.0 36.918915 94-95 TCCTAGA 1530 4.2808097E-8 36.539722 94-95 >>END_MODULE