FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l04n02_7331.34200000035c1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l04n02_7331.34200000035c1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19885260
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA12800.092.81232594-95
GTTCTCG16100.069.44816694-95
ACATCCG21300.068.89796494-95
CTTTGTG1580200.068.105971
CTAGTTA26300.066.4279794-95
TTTGTGT1681800.064.188072
TTGTGTT1915800.057.836893
AACTTTG174400.056.1043742
TAACTTT177200.054.4566231
ACTTTGT405050.054.214281
TGTGTTT2063000.053.957414
CTCTCGG15700.053.41316294-95
GTGTTTG2376950.046.80445
CGTCGTA28450.044.21370794-95
CATTCGA18001.8189894E-1242.70580394-95
TCTCGCG12052.7222086E-740.59548694-95
GGATGTA17809.458745E-1139.25967494-95
GAGCGTA33600.037.43690594-95
CCGCTAA26500.036.91891594-95
TCCTAGA15304.2808097E-836.53972294-95
Produced by FastQC (version 0.11.2)