FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l04n02_6957.34200000035bd7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l04n02_6957.34200000035bd7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26521818
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTAG14400.092.0044294-95
AGATCGA38800.077.27772594-95
TCTCGCG11150.062.5378294-95
TCCTAGA17150.060.9880594-95
CTTTGTG4642750.059.47451
TAACTTT691100.057.5670851
CGCTAGG6201.0415521E-556.233694-95
TTTGTGT4955150.055.9540022
AACTTTG737200.054.6864362
TCATACG13403.6379788E-1252.03706494-95
TTGTGTT5498800.051.5921133
ACTTTGT1562250.050.9622271
TGTGTTT5759700.049.4479644
GACTAAA33200.048.30669894-95
CGTCGTA27100.046.31490794-95
GTTCTCG40000.045.32428494-95
GTGTTTG6421600.044.3470955
ACATCCA81050.043.8767894-95
CTCTAGG8105.059622E-543.04300394-95
GAGATCA27900.042.4876194-95
Produced by FastQC (version 0.11.2)