FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l04n02_6929.34200000035b94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l04n02_6929.34200000035b94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15584665
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA19250.091.48579494-95
CTTTGTG789850.066.028691
TTTGTGT872650.059.9875032
TTGTGTT981500.054.595713
TGTGTTT1072000.050.200174
ACTTTGT241550.048.1722641
GTGTTTG1173350.045.8471645
GTTCTCG18101.8189894E-1242.81130694-95
ACATCCG24250.040.66873694-95
CTCTCGG15303.9879524E-836.83349694-95
TCTCGCG10051.6969773E-435.046894-95
GTTTGAG978200.033.147172
AACTTTG117850.032.9119222
TAACTTT117600.032.7841531
TGTTTGA1881750.032.77661
TTTGAGG364750.030.5357698
TTTGAGT426550.028.6207053
TTGAGGG196200.027.8033839
AGTTCTG34905.456968E-1226.23990894-95
TCCTAGG13559.769705E-425.99411894-95
Produced by FastQC (version 0.11.2)