FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l04n02_6777.34200000035b44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l04n02_6777.34200000035b44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12718229
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA13150.073.86700494-95
CTTTGTG1091050.067.1976241
TTTGTGT1231850.059.800342
CTAGTTA10753.092282E-1158.0874494-95
GTTCTCG15850.056.90654894-95
TTGTGTT1341650.056.1485753
TGTGTTT1449050.052.180964
GTGTTTG1526500.049.4919055
ACTTTGT286300.048.4060331
AACTTTG136000.048.07872
TAACTTT138500.046.975621
TCTATAG11101.5104524E-743.75454794-95
CTCTCGG13301.3253157E-841.7336794-95
TCCCTCG10154.0970062E-641.0141294-95
ACATCCG17303.3360266E-936.09479594-95
TCTCGCG11751.1187394E-535.4292294-95
TTTGAGG420350.034.8253758
GTTTGAG1169500.033.6642537
TGTTTGA2247500.033.5800866
CAGTCTA10552.4673133E-432.8825794-95
Produced by FastQC (version 0.11.2)