FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l04n02_30082.34200000035d35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l04n02_30082.34200000035d35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15063215
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGAGTAGCGAAAGACAGGTGAGAATCCTGTCCACCGTAAGACTAAG163760.10871517136281994No Hit
CTTTGTGTTTGAGGGGTCCATCCCGGATTACCAATCTCAGATAAACTCCG160700.10668373252323625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCCG13850.0138.4495494-95
AGATCGA11700.0127.4708894-95
GTTCTCG11300.0113.1283994-95
CTCTCGG11400.087.2169294-95
GAGCGTA21100.084.1463194-95
CTCGCTG11050.083.5523494-95
CTTTGTG1589150.077.0484541
TTTGTGT1706900.071.9569552
CAGTCTA8251.5643309E-1068.8673894-95
TTGTGTT1922050.065.66713
TGTGTTT1988650.063.734984
GTGTTTG2161200.058.6109965
TCTCGCG8751.931221E-856.81558694-95
GCGTCGG9604.0115992E-851.78504694-95
GGCCTAG5704.2510193E-449.83823894-95
GGGTCCG4400.0095692648.4223894-95
ACTTTGT237100.047.763361
GACCTAG12305.4078555E-946.1915494-95
AGTTCTG21650.045.92484394-95
TCCCTCG6256.694569E-445.45247394-95
Produced by FastQC (version 0.11.2)