FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l04n02_30076.34200000035ce3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l04n02_30076.34200000035ce3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16088559
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT228870.14225636988371676No Hit
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC214580.1333742816867564No Hit
TGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT171180.1063985904517614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA4850.0217.3390594-95
AGATCGA8900.0205.2918194-95
CTATATG6050.0139.3843894-95
GCTATAA8600.0138.9116794-95
ATATTAA18950.0118.6667594-95
TATGCTA12900.0114.3978494-95
CTAGCCG3151.77517E-7111.5443894-95
GCGTCGG5403.6379788E-12104.10808694-95
CTTATAG4801.8007995E-10102.4814194-95
GTTCTCG4309.888026E-998.055394-95
ATATTAG15850.097.5397694-95
TATTATA16400.094.2686194-95
TTAGGCA8250.093.6972794-95
ATCGGAC5354.274625E-1091.9459394-95
CCTAGCG3906.335722E-790.09354494-95
TGCCCGA5505.329639E-1089.4383294-95
CTATACG4952.6275302E-885.1793594-95
ATTATGA17000.082.6740794-95
AGAGCGA18100.081.5321694-95
AATCACG6101.2114469E-980.64110694-95
Produced by FastQC (version 0.11.2)