##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l04n01_8033.34100000035d6e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17579714 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.73002985145265 33.0 33.0 33.0 33.0 33.0 2 32.76507717929882 33.0 33.0 33.0 33.0 33.0 3 32.79392372367378 33.0 33.0 33.0 33.0 33.0 4 36.6046276975837 37.0 37.0 37.0 37.0 37.0 5 36.62702237362906 37.0 37.0 37.0 37.0 37.0 6 36.636310181155395 37.0 37.0 37.0 37.0 37.0 7 36.61440732198487 37.0 37.0 37.0 37.0 37.0 8 36.564289726215115 37.0 37.0 37.0 37.0 37.0 9 36.589991281996966 37.0 37.0 37.0 37.0 37.0 10-11 36.58214362873025 37.0 37.0 37.0 37.0 37.0 12-13 36.55712035474525 37.0 37.0 37.0 37.0 37.0 14-15 38.63722581038577 40.0 37.0 40.0 37.0 40.0 16-17 38.61102103253785 40.0 37.0 40.0 37.0 40.0 18-19 38.5710930792162 40.0 37.0 40.0 37.0 40.0 20-21 38.517193254679796 40.0 37.0 40.0 37.0 40.0 22-23 38.469449360780274 40.0 37.0 40.0 37.0 40.0 24-25 38.42038951828226 40.0 37.0 40.0 37.0 40.0 26-27 38.31716889705942 40.0 37.0 40.0 37.0 40.0 28-29 38.25587114215851 40.0 37.0 40.0 37.0 40.0 30-31 38.19277150925208 40.0 37.0 40.0 37.0 40.0 32-33 38.090072853289875 40.0 37.0 40.0 37.0 40.0 34-35 38.004575785476376 40.0 37.0 40.0 37.0 40.0 36-37 37.91804639142593 40.0 37.0 40.0 35.0 40.0 38-39 37.79744238159961 40.0 37.0 40.0 33.0 40.0 40-41 37.62999437874814 40.0 37.0 40.0 33.0 40.0 42-43 37.52073076956769 40.0 37.0 40.0 33.0 40.0 44-45 37.40230060056722 40.0 37.0 40.0 33.0 40.0 46-47 37.24278867676687 38.5 37.0 40.0 33.0 40.0 48-49 37.04679481702603 37.0 37.0 40.0 33.0 40.0 50-51 36.85584660251014 37.0 37.0 40.0 33.0 40.0 52-53 36.618436909724466 37.0 37.0 40.0 33.0 40.0 54-55 36.36984691559829 37.0 37.0 40.0 33.0 40.0 56-57 36.1076549652628 37.0 37.0 40.0 33.0 40.0 58-59 35.8634966131986 37.0 37.0 40.0 33.0 40.0 60-61 35.57132763934612 37.0 33.0 38.5 33.0 40.0 62-63 35.256070548132925 37.0 33.0 37.0 30.0 40.0 64-65 35.02479576743967 37.0 33.0 37.0 27.0 40.0 66-67 34.7481780989156 37.0 33.0 37.0 27.0 40.0 68-69 34.48271826265206 37.0 33.0 37.0 27.0 37.0 70-71 34.20688812116056 37.0 33.0 37.0 27.0 37.0 72-73 33.97124933318028 37.0 33.0 37.0 27.0 37.0 74-75 33.73561691617964 37.0 33.0 37.0 27.0 37.0 76-77 30.955993112288404 33.0 30.0 35.0 24.5 37.0 78-79 33.46861572947091 35.0 33.0 37.0 27.0 37.0 80-81 33.920568645200945 37.0 33.0 37.0 27.0 37.0 82-83 33.90350554053382 37.0 33.0 37.0 27.0 37.0 84-85 33.84112858718862 37.0 33.0 37.0 27.0 37.0 86-87 33.737855007197496 37.0 33.0 37.0 27.0 37.0 88-89 33.55043824945048 37.0 33.0 37.0 27.0 37.0 90-91 33.41554487177664 37.0 33.0 37.0 27.0 37.0 92-93 33.3008329942114 37.0 33.0 37.0 27.0 37.0 94-95 33.192088107918025 37.0 33.0 37.0 27.0 37.0 96-97 33.07473673917562 35.0 33.0 37.0 27.0 37.0 98-99 32.92622798072824 33.0 33.0 37.0 27.0 37.0 100-101 31.069891040320677 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13595417871926685 2306 2 0.10163207997128865 2306 3 0.09725415093794254 2306 4 -0.14898974487556416 2306 5 -0.0640964011513887 2306 6 0.08822020747859938 2306 7 0.12135338952833763 2306 8 0.17027851188705512 2306 9 0.1332637563783834 2306 10-11 0.110783917251986 2306 12-13 0.10578346519521631 2306 14-15 0.21201765039186427 2306 16-17 0.20669416553994324 2306 18-19 0.1537997979844974 2306 20-21 0.1479765342188344 2306 22-23 0.17124620991741324 2306 24-25 0.19551704616468868 2306 26-27 0.20108773499179478 2306 28-29 0.2213441174998394 2306 30-31 0.19829742773823966 2306 32-33 0.215479084173829 2306 34-35 0.19081322358464803 2306 36-37 0.153548645256258 2306 38-39 0.1517084062270797 2306 40-41 0.25000775230481764 2306 42-43 0.2059160723684812 2306 44-45 0.14144967394766184 2306 46-47 0.06632732624682092 2306 48-49 0.1032840339893184 2306 50-51 0.057769599600234756 2306 52-53 0.06402468295878805 2306 54-55 0.08085916268269955 2306 56-57 0.06369704195063264 2306 58-59 -0.01300604730887045 2306 60-61 -0.08999700133157518 2306 62-63 0.020173112719490405 2306 64-65 0.01870648456970514 2306 66-67 -0.07960577545283343 2306 68-69 -0.2025586233845047 2306 70-71 -0.17523593814335214 2306 72-73 -0.07263271226656798 2306 74-75 -0.18786430470344584 2306 76-77 -0.7841240333591486 2306 78-79 -0.12306722968737205 2306 80-81 0.11321758161763995 2306 82-83 -0.08574032270853138 2306 84-85 -0.11560425685724596 2306 86-87 -0.06382271881061286 2306 88-89 -0.20112365035061686 2306 90-91 -0.2198832457724862 2306 92-93 -0.31343817360890114 2306 94-95 -0.3233229537728448 2306 96-97 -0.20052655987789336 2306 98-99 -0.11308926887739545 2306 100-101 -0.4273606021179255 2307 1 0.10977013697561233 2307 2 0.0918721925628816 2307 3 0.08840771451697549 2307 4 -0.1708743118522662 2307 5 -0.016828836863176377 2307 6 0.08377067721998799 2307 7 0.072542117551599 2307 8 0.12845174227435052 2307 9 0.08071232425927377 2307 10-11 0.07205837325398079 2307 12-13 0.04179710565810524 2307 14-15 0.15838701066772387 2307 16-17 0.08167248152530249 2307 18-19 0.07577946820040182 2307 20-21 0.11681669592962862 2307 22-23 0.08063846474782821 2307 24-25 0.07670522498619903 2307 26-27 0.08986326343978135 2307 28-29 0.0689690292844034 2307 30-31 0.03133505653327262 2307 32-33 0.05991415183833482 2307 34-35 0.08585211687744732 2307 36-37 0.004114588963737731 2307 38-39 0.004591160804253036 2307 40-41 0.053910388711265966 2307 42-43 -0.024818076319256477 2307 44-45 -0.034839892024429275 2307 46-47 -0.018831815891601877 2307 48-49 -0.031036861334122534 2307 50-51 -0.10990306184072551 2307 52-53 -0.12739355939605446 2307 54-55 -0.08618728551917343 2307 56-57 -0.21509529868897914 2307 58-59 -0.25424327847373007 2307 60-61 -0.2482698714016749 2307 62-63 -0.24948248105732773 2307 64-65 -0.32650437066200766 2307 66-67 -0.3219515820813754 2307 68-69 -0.4254596491762115 2307 70-71 -0.42470750662049994 2307 72-73 -0.4536699323595954 2307 74-75 -0.4486406416412265 2307 76-77 -0.855351676200943 2307 78-79 -0.31365703505435505 2307 80-81 -0.19445389903112442 2307 82-83 -0.22954994024243547 2307 84-85 -0.2526614616810221 2307 86-87 -0.2782485553948817 2307 88-89 -0.3269177959950369 2307 90-91 -0.43859713582081383 2307 92-93 -0.4048556916860022 2307 94-95 -0.36840784207899446 2307 96-97 -0.3448580203708005 2307 98-99 -0.28844993598008273 2307 100-101 -0.752171254558629 2201 1 0.08224189115365022 2201 2 0.07331520024580129 2201 3 0.07201589612637349 2201 4 -0.1356635341282626 2201 5 -0.026326642033197345 2201 6 0.03250448606586076 2201 7 0.09118381457496127 2201 8 0.13376106900234674 2201 9 0.1000637131750679 2201 10-11 0.09371957988412305 2201 12-13 0.08514256819125876 2201 14-15 0.16186773301139823 2201 16-17 0.14069560333950903 2201 18-19 0.12292820966465712 2201 20-21 0.11275002360762443 2201 22-23 0.12027025915778466 2201 24-25 0.11583803908156654 2201 26-27 0.1503352761834762 2201 28-29 0.15681617202412923 2201 30-31 0.13375636187981144 2201 32-33 0.177870721961078 2201 34-35 0.1042336807946711 2201 36-37 0.05027787800673167 2201 38-39 0.11718982125355382 2201 40-41 0.2104341997308623 2201 42-43 0.08717320718400856 2201 44-45 0.04892729430028453 2201 46-47 0.0046795396489045515 2201 48-49 0.026093947309917098 2201 50-51 0.09259686182472393 2201 52-53 0.05817594840188178 2201 54-55 0.01733213232940045 2201 56-57 0.03069780539492939 2201 58-59 -0.04889314489296481 2201 60-61 -0.06853429052833349 2201 62-63 0.039668397837075986 2201 64-65 -0.018906761341057177 2201 66-67 -0.143557906206226 2201 68-69 -0.1921744316898213 2201 70-71 -0.14706134977836172 2201 72-73 -0.14592470894325515 2201 74-75 -0.24245801472068962 2201 76-77 -0.8540956985158381 2201 78-79 -0.12064106034865318 2201 80-81 -0.02958527941625988 2201 82-83 0.002680285142929506 2201 84-85 -0.13081607717526111 2201 86-87 -0.043258130600463574 2201 88-89 -0.0793623277357014 2201 90-91 -0.10931771581042682 2201 92-93 -0.20436253599555698 2201 94-95 -0.16268778373495252 2201 96-97 -0.06501356166051409 2201 98-99 -0.11733628007041119 2201 100-101 -0.4694797600083547 1101 1 0.0865644209913583 1101 2 0.07807809342280336 1101 3 0.05994989814693241 1101 4 -0.1211964847068856 1101 5 -0.0235956729148441 1101 6 0.04009861745894483 1101 7 0.046585713008362006 1101 8 0.15916609146745486 1101 9 0.0775530545265326 1101 10-11 0.05092852345895693 1101 12-13 0.03579928588480641 1101 14-15 0.11578337500223768 1101 16-17 0.09884769919248271 1101 18-19 0.1303680560267111 1101 20-21 0.07715824012383621 1101 22-23 0.052661027248234404 1101 24-25 0.045027408467817054 1101 26-27 0.10919945642369555 1101 28-29 0.05283700663918722 1101 30-31 0.052402095294048934 1101 32-33 0.05939636711764962 1101 34-35 0.07175600348617905 1101 36-37 0.05775898097491705 1101 38-39 0.031192617511038634 1101 40-41 0.06543300577066447 1101 42-43 0.04992935393556763 1101 44-45 0.04835141078228844 1101 46-47 0.10206854393013032 1101 48-49 0.03604874733307639 1101 50-51 -0.05803070783039033 1101 52-53 -0.09710959636084482 1101 54-55 -0.04204841493654499 1101 56-57 -0.004896285678114509 1101 58-59 -0.0409655813360672 1101 60-61 -0.041058684643253684 1101 62-63 -0.04644228812405515 1101 64-65 -0.11662320674371074 1101 66-67 -0.16135498070830323 1101 68-69 -0.1484541071427472 1101 70-71 -0.29539030467832816 1101 72-73 -0.09422804767152115 1101 74-75 -0.2014856524178228 1101 76-77 -0.6703617155507509 1101 78-79 0.015865945859211195 1101 80-81 -0.0026474190678555942 1101 82-83 0.04559851001165782 1101 84-85 0.013938030941879731 1101 86-87 0.062344901753320414 1101 88-89 0.051103656023578026 1101 90-91 -0.021186692012832964 1101 92-93 -0.1444178845375106 1101 94-95 -0.12941268951289686 1101 96-97 0.004053221826161746 1101 98-99 -0.07899987020888233 1101 100-101 -0.35335182274172183 2304 1 0.08947683974484733 2304 2 0.06583026541223802 2304 3 0.060240034126834985 2304 4 -0.21958229385647599 2304 5 -0.07590629603881638 2304 6 0.048541411103094845 2304 7 0.09956741359837196 2304 8 0.15076678859809078 2304 9 0.09610638062558507 2304 10-11 0.06758052623920463 2304 12-13 0.07192648903140508 2304 14-15 0.07755704854347556 2304 16-17 0.13438501662906788 2304 18-19 0.0971871486581648 2304 20-21 0.08577290087209377 2304 22-23 0.09982496375346273 2304 24-25 0.10198991976105987 2304 26-27 0.12012404731598991 2304 28-29 0.05876281545269535 2304 30-31 0.08373825325671191 2304 32-33 0.14892961335095123 2304 34-35 0.1490754244034136 2304 36-37 0.14453918935290488 2304 38-39 0.07840132393148735 2304 40-41 0.13649313474249425 2304 42-43 0.14435624588031715 2304 44-45 0.0866320984654223 2304 46-47 0.11576478529310918 2304 48-49 0.06639189735562212 2304 50-51 -0.03451339676059462 2304 52-53 -0.0139354913264782 2304 54-55 0.045430697822212096 2304 56-57 -0.04288329618203335 2304 58-59 -0.08776329320247811 2304 60-61 -0.0713082150506068 2304 62-63 -0.053110025319057286 2304 64-65 9.0683094882138E-5 2304 66-67 2.889592682961961E-4 2304 68-69 -0.20732818385469187 2304 70-71 -0.136242286353216 2304 72-73 -0.10325432234708387 2304 74-75 -0.16585454032577474 2304 76-77 -0.3274606990102171 2304 78-79 0.03626232930820805 2304 80-81 0.059083750207612695 2304 82-83 -0.01248688910206397 2304 84-85 -0.012927962069291254 2304 86-87 0.05033462134204569 2304 88-89 0.09773494498710278 2304 90-91 0.030061162891094284 2304 92-93 -0.07681357112874565 2304 94-95 0.014539571314415412 2304 96-97 0.062257482259660435 2304 98-99 0.019898896696147972 2304 100-101 -0.32527656626912815 2202 1 0.09841918409163242 2202 2 0.05626698340915226 2202 3 0.02441474722088799 2202 4 -0.22199866015613878 2202 5 -0.06784125849847555 2202 6 0.025857507229723353 2202 7 0.03434001471555348 2202 8 0.15924617566800237 2202 9 0.0330035351364657 2202 10-11 0.05903636351083463 2202 12-13 0.06897045524851109 2202 14-15 0.030802990114864315 2202 16-17 0.01284710270266487 2202 18-19 0.07225806238130161 2202 20-21 0.034392774941125026 2202 22-23 0.05133743564517346 2202 24-25 0.06055894013276486 2202 26-27 0.06374423825277375 2202 28-29 0.04565215186737248 2202 30-31 0.02281882051257611 2202 32-33 0.0382492422701759 2202 34-35 -0.017438410568864526 2202 36-37 0.01196102811188382 2202 38-39 -0.03682277937686251 2202 40-41 0.05413707799667122 2202 42-43 0.05204607305059028 2202 44-45 -0.022213636995466857 2202 46-47 0.048196681857490375 2202 48-49 0.038949842966140125 2202 50-51 -6.496756427054606E-4 2202 52-53 -0.01738689309853214 2202 54-55 0.014233868656639004 2202 56-57 -0.0014466852444883216 2202 58-59 -0.010551862873718676 2202 60-61 -0.07972819981699075 2202 62-63 0.003606380848694357 2202 64-65 0.02054817490217431 2202 66-67 0.01596269859310695 2202 68-69 -0.09035068805593482 2202 70-71 -0.11177736807430705 2202 72-73 -0.03144604899311787 2202 74-75 -0.1400697030704876 2202 76-77 -0.14466389069400876 2202 78-79 0.053649503166987245 2202 80-81 0.06732575897412829 2202 82-83 0.12056612443846859 2202 84-85 0.03726637281113909 2202 86-87 0.05370925674024818 2202 88-89 0.05010916945575872 2202 90-91 0.037886176015980766 2202 92-93 0.05994508521082764 2202 94-95 0.03626963291823415 2202 96-97 0.06681768403851862 2202 98-99 -0.005585338483768965 2202 100-101 -0.07578837260541249 1102 1 0.05840804825643886 1102 2 0.048361505442528596 1102 3 0.03299317350484188 1102 4 -0.23086262580221728 1102 5 -0.09094590582191131 1102 6 -0.004427112476356854 1102 7 0.03833599329648507 1102 8 0.11910356208240813 1102 9 0.040463860359444936 1102 10-11 0.02687407489907656 1102 12-13 0.02149656625223173 1102 14-15 0.03291830283405517 1102 16-17 0.008418891545950657 1102 18-19 0.008547190247476522 1102 20-21 -0.007529326961993377 1102 22-23 0.03834739968785783 1102 24-25 0.05233134033201026 1102 26-27 0.0840721779110396 1102 28-29 0.06634969367904375 1102 30-31 0.03730150014424538 1102 32-33 0.016243182152045677 1102 34-35 0.06973232988189437 1102 36-37 0.007644771238105363 1102 38-39 0.0052071629631669225 1102 40-41 0.09948061758978355 1102 42-43 0.06929810925058177 1102 44-45 -0.01071615051694863 1102 46-47 0.023272626531102958 1102 48-49 -0.042449242622005556 1102 50-51 -0.03061891522401794 1102 52-53 -0.025118960920551103 1102 54-55 -0.019423883728585167 1102 56-57 0.022359407430698752 1102 58-59 -0.06689080172598239 1102 60-61 -0.0812033096643674 1102 62-63 -0.001790179683823112 1102 64-65 -0.048892838774094116 1102 66-67 -0.04590688718182179 1102 68-69 -0.15113596013340924 1102 70-71 -0.22143421410620334 1102 72-73 -0.14434630122185865 1102 74-75 -0.1718688947881688 1102 76-77 -0.21181781358838592 1102 78-79 -0.07996230678532612 1102 80-81 -0.05407367417632969 1102 82-83 -0.07321674884384777 1102 84-85 -0.11279818765863325 1102 86-87 -0.13617470165930712 1102 88-89 -0.167498197312149 1102 90-91 -0.2102036902970923 1102 92-93 -0.2680553599303792 1102 94-95 -0.28317253609517223 1102 96-97 -0.2263890585628232 1102 98-99 -0.18298684224443917 1102 100-101 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50-51 -0.19464378801732352 1213 52-53 -0.23932774603723317 1213 54-55 -0.13223223480892443 1213 56-57 -0.11644539382842822 1213 58-59 -0.190263858583414 1213 60-61 -0.19474865090787574 1213 62-63 -0.06677882434727422 1213 64-65 -0.05989637587394725 1213 66-67 0.06144203700506523 1213 68-69 0.12735506202814406 1213 70-71 -0.022645202256498465 1213 72-73 0.02608604919230828 1213 74-75 0.12786286139360215 1213 76-77 0.5719833306265549 1213 78-79 0.05195302956628467 1213 80-81 -0.13664905504620606 1213 82-83 -0.2194465741733893 1213 84-85 -0.10244474980876106 1213 86-87 -0.0696949820382855 1213 88-89 0.027542408320087475 1213 90-91 0.041355997342499506 1213 92-93 0.17476943247131516 1213 94-95 0.10753679510843028 1213 96-97 0.12510934516161853 1213 98-99 0.22170212050915694 1213 100-101 0.45951757284237615 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 34.0 8 559.0 9 1607.0 10 1146.0 11 447.0 12 383.0 13 802.0 14 1831.0 15 4237.0 16 7802.0 17 11783.0 18 15937.0 19 20710.0 20 26234.0 21 32955.0 22 40753.0 23 50975.0 24 63500.0 25 80355.0 26 101523.0 27 128061.0 28 163430.0 29 208499.0 30 268669.0 31 353134.0 32 476909.0 33 678655.0 34 1070895.0 35 2029297.0 36 4370251.0 37 6164236.0 38 1204035.0 39 67.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 20.292711788249825 23.940835910450787 30.12951172117842 25.63694058012097 2 25.497388637835634 23.84917638591845 30.276823616129363 20.376611360116552 3 21.548746469936884 21.91496972021274 35.26620512711413 21.270078682736248 4 21.483544113055633 22.397055457603283 34.30080246029223 21.818597969048856 5 27.376492018015764 21.674379913120315 30.43844171753875 20.51068635132517 6 27.513752499045207 22.0757118119214 30.392223673263395 20.018312015769997 7 27.428028692616955 25.81770101606886 26.312953669212135 20.441316622102043 8 25.452780403594733 23.39008473061621 30.059692666217437 21.09744219957162 9 22.67985133319006 22.351484216409894 33.34778370114553 21.62088074925451 10-11 26.24772564455856 23.295955575138745 28.62874384816219 21.827574932140504 12-13 25.57175668076225 26.29802566693285 25.658195400940166 22.47202225136474 14-15 25.44468941430701 24.409817600269765 26.89746621866164 23.248026766761587 16-17 24.972035950072907 24.347028626290506 26.66018628061867 24.020749143017913 18-19 24.71912796761085 24.772720989658875 27.078634498832006 23.42951654389827 20-21 23.833289499293343 25.178294376973902 27.422425623797448 23.565990499935307 22-23 23.891268409508772 25.206852951124475 27.064334316781725 23.83754432258503 24-25 24.293552215923423 25.227546363951088 26.72195349708192 23.756947923043573 26-27 24.04921661410419 25.22550708162829 26.995655333186875 23.729620971080646 28-29 24.09296305958106 25.56570601774295 26.503881121160443 23.837449801515543 30-31 24.13925220853991 25.30487412935159 26.59417269245677 23.961700969651726 32-33 24.176624830187794 25.639049076680088 26.299000086121993 23.885326007010125 34-35 23.951908432640032 25.737676961070015 26.428470338027115 23.88194426826284 36-37 24.086663205212602 25.649127163213247 26.480678217456393 23.783531414117757 38-39 24.17212580170431 25.485094343427694 26.41845518398834 23.924324670879656 40-41 23.943190444751885 25.909622537753744 26.196191577699253 23.950995439795115 42-43 23.987836609900842 25.758890738141538 26.215221229031084 24.038051422926536 44-45 23.986007394659552 25.563797568037792 26.400005711128177 24.050189326174475 46-47 23.87552209324907 25.680218688426898 26.449744859330476 23.994514358993555 48-49 23.910447909770994 25.45136901414059 26.353124375611948 24.285058700476473 50-51 23.84299811786552 25.643685497700762 26.64390967916112 23.8694067052726 52-53 24.09821683893681 25.737481500933875 26.41229864651706 23.752003013612256 54-55 23.757522854039387 25.74985481157305 26.28259116449382 24.210031169893742 56-57 24.007142733816003 25.94618993752099 26.31034102093887 23.736326307724134 58-59 23.880648174209938 25.973550603177838 26.352517440237257 23.793283782374967 60-61 24.14477649340259 25.961974454519776 25.99644410009878 23.896804951978854 62-63 23.844357869083414 26.093335800955813 26.248443906057535 23.81386242390324 64-65 24.01419845480109 26.053108760541498 26.096120073523686 23.836572711133723 66-67 24.299419548962863 25.975823927007557 26.080359728315006 23.644396795714574 68-69 24.148552734513903 25.745966858687147 26.38268686437664 23.722793542422302 70-71 24.078573464310917 26.16441352287974 26.12338132191908 23.633631690890258 72-73 23.92851991865212 25.88085192814328 26.339720983263604 23.850907169940996 74-75 24.01771220583758 25.913785853444754 26.30636932660351 23.762132614114154 76-77 23.937015677611214 25.914150886209146 26.18365058665916 23.965182849520485 78-79 24.093137569320746 25.872602941523237 25.99136841208825 24.042891077067765 80-81 24.06684048641237 25.767138010462293 26.24934245882258 23.91667904430276 82-83 23.889720132026156 26.217254957842677 26.189784825354323 23.703240084776844 84-85 23.909414901406077 26.181338477077244 26.103299958390835 23.805946663125848 86-87 23.79324408013655 26.11014000709504 26.244761393300198 23.851854519468215 88-89 23.684072191147678 26.874953847106113 25.828183457369175 23.612790504377028 90-91 23.82516634988358 26.23668665349006 25.92314978065876 24.014997215967597 92-93 23.728679189024717 26.558178325708575 25.840763592525022 23.872378892741683 94-95 23.663448556475714 26.7664468284173 25.485421020129923 24.08468359497706 96-97 23.675017516495572 26.44226968954936 25.641288054698624 24.24142473925644 98-99 23.971613347634147 26.336404427532223 25.476037426588917 24.215944798244713 100-101 24.098756019868524 26.83450178833412 25.065805608783375 24.00093658301398 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 16692.0 1 9994.5 2 4462.5 3 5016.0 4 4648.0 5 3859.0 6 2581.0 7 2313.0 8 2406.0 9 2536.0 10 2537.0 11 2492.5 12 2502.0 13 2640.0 14 2816.5 15 3076.5 16 3286.5 17 3482.0 18 3840.5 19 4280.5 20 4917.0 21 5707.0 22 6511.0 23 7636.0 24 8933.5 25 10388.0 26 12438.5 27 14925.0 28 18099.5 29 21701.0 30 25800.0 31 31647.0 32 47792.5 33 77984.0 34 115993.0 35 149205.0 36 184164.5 37 248021.0 38 318533.5 39 365336.5 40 414226.0 41 463435.0 42 506981.5 43 570532.5 44 716011.0 45 894788.5 46 1048670.0 47 1186645.5 48 1260406.0 49 1234596.0 50 1103861.5 51 993138.0 52 895939.5 53 803125.5 54 751244.5 55 687938.5 56 603742.0 57 457379.0 58 309576.0 59 219203.5 60 161977.5 61 132586.5 62 115533.0 63 88018.5 64 56687.0 65 36999.0 66 24553.5 67 16219.5 68 12161.0 69 9603.0 70 7825.5 71 6789.5 72 6199.5 73 5457.5 74 4660.5 75 3761.5 76 2896.0 77 2544.5 78 2384.5 79 2039.0 80 1492.0 81 934.0 82 620.0 83 534.5 84 439.0 85 338.5 86 296.5 87 290.5 88 291.5 89 285.0 90 239.0 91 171.0 92 136.5 93 114.5 94 94.0 95 90.5 96 73.5 97 43.5 98 24.0 99 12.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12689057398772244 2 0.0 3 0.0 4 0.34041509435250195 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.550699744034516E-5 12-13 0.0011831819334489743 14-15 2.8441873400215727E-6 16-17 0.0 18-19 0.0 20-21 8.504120146664502E-4 22-23 4.3231647568327903E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.002255440560637107 38-39 0.00741479639543624 40-41 0.006982479919752961 42-43 0.0013652099232103548 44-45 0.0 46-47 0.0 48-49 8.532562020064718E-6 50-51 0.0011661168094088447 52-53 0.012158900878592224 54-55 0.00963895089533311 56-57 0.00893359243500776 58-59 0.012437631237914337 60-61 0.0027247314717406663 62-63 2.2469079986170423E-4 64-65 0.003515415552266664 66-67 0.011399502858806462 68-69 0.019704529891669455 70-71 0.02555786743743385 72-73 0.019050366803464492 74-75 0.003697443542028044 76-77 0.014303418132968489 78-79 0.007807294248359217 80-81 0.005500658315601721 82-83 0.003535324863646815 84-85 0.010557623406160076 86-87 0.015020153342653924 88-89 0.012901233774337852 90-91 0.03063474183937236 92-93 0.013842659783884994 94-95 0.014064506396406678 96-97 0.007301028901835377 98-99 0.012269824184853066 100-101 0.13885038175251316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7579714E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.908980338002674 #Duplication Level Percentage of deduplicated Percentage of total 1 80.24629163071019 19.988533004269886 2 9.506427437119338 4.735908282317095 3 3.1619622801270806 2.362837687955747 4 1.6845088075153958 1.6783758706237333 5 0.9628166166814018 1.1991390087009648 6 0.48917171396220616 0.7310861162994998 7 0.49147808519288116 0.8569552572440078 8 0.3154437661396696 0.6285906054814837 9 0.21927216080082856 0.4915661347853279 >10 2.082349053083098 10.526570144324216 >50 0.3271562417200081 5.809229098650583 >100 0.38724162391301703 20.8680902894938 >500 0.08446641869948138 14.88017171951825 >1k 0.04122236870645768 14.91864954465626 >5k 1.688692641185298E-4 0.25788722839393713 >10k+ 2.292636527465673E-5 0.06641000728513786 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.137674936008629E-4 0.0 0.0 2 1.137674936008629E-4 0.0 0.0 3 1.137674936008629E-4 0.0 0.0 4 1.1945586828090605E-4 0.0 5.688374680043145E-6 5 1.3083261764099233E-4 0.0 5.688374680043145E-6 6 1.3652099232103548E-4 0.0 5.688374680043145E-6 7 1.7065124040129435E-4 0.0 5.688374680043145E-6 8 1.763396150813375E-4 0.0 5.688374680043145E-6 9 1.8202798976138065E-4 0.0 5.688374680043145E-6 10-11 2.5597686060194153E-4 0.0 5.688374680043145E-6 12-13 3.6974435420280443E-4 0.0 5.688374680043145E-6 14-15 5.290188452440125E-4 0.0 5.688374680043145E-6 16-17 7.565538324457383E-4 0.0 5.688374680043145E-6 18-19 0.0010295958170878092 0.0 5.688374680043145E-6 20-21 0.0013083261764099234 0.0 5.688374680043145E-6 22-23 0.0016553170318925553 0.0 5.688374680043145E-6 24-25 0.0020591916341756184 0.0 5.688374680043145E-6 26-27 0.0025768337300595447 0.0 5.688374680043145E-6 28-29 0.003029059517122975 0.0 5.688374680043145E-6 30-31 0.003589364423107225 0.0 5.688374680043145E-6 32-33 0.004195176326531819 0.0 5.688374680043145E-6 34-35 0.004988704594397838 0.0 5.688374680043145E-6 36-37 0.005802142173644009 0.0 5.688374680043145E-6 38-39 0.006845958927431926 0.0 5.688374680043145E-6 40-41 0.008162817665861914 0.0 5.688374680043145E-6 42-43 0.009661704394053281 0.0 5.688374680043145E-6 44-45 0.011493361041027175 0.0 5.688374680043145E-6 46-47 0.013728892290284131 0.0 5.688374680043145E-6 48-49 0.016393895827884343 0.0 5.688374680043145E-6 50-51 0.01976710201314993 0.0 5.688374680043145E-6 52-53 0.024033383023182288 0.0 5.688374680043145E-6 54-55 0.029169985359261248 0.0 5.688374680043145E-6 56-57 0.0359533721652127 0.0 5.688374680043145E-6 58-59 0.04410196889437451 0.0 5.688374680043145E-6 60-61 0.05411350833125044 0.0 5.688374680043145E-6 62-63 0.06665068612606553 0.0 5.688374680043145E-6 64-65 0.08280567021738806 0.0 5.688374680043145E-6 66-67 0.10245616054959711 0.0 5.688374680043145E-6 68-69 0.12722049971916494 0.0 5.688374680043145E-6 70-71 0.15797185323947818 0.0 5.688374680043145E-6 72-73 0.1959673519148264 0.0 5.688374680043145E-6 74-75 0.24266606385064057 2.8441873400215727E-6 5.688374680043145E-6 76-77 0.3004030668530785 5.688374680043145E-6 5.688374680043145E-6 78-79 0.3699662008153261 5.688374680043145E-6 5.688374680043145E-6 80-81 0.45581799567387726 5.688374680043145E-6 5.688374680043145E-6 82-83 0.5592582450431218 5.688374680043145E-6 1.137674936008629E-5 84-85 0.6816322495348901 5.688374680043145E-6 1.137674936008629E-5 86-87 0.8266516736279099 5.688374680043145E-6 1.137674936008629E-5 88-89 0.9970640022926425 5.688374680043145E-6 1.137674936008629E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 125405 0.0 65.366425 1 TTTGTGT 135915 0.0 60.24082 2 ACTTTGT 37060 0.0 57.09938 1 TTGTGTT 152725 0.0 55.188713 3 TGTGTTT 160325 0.0 52.86987 4 GTGTTTG 177010 0.0 47.87735 5 TTTGAGG 57475 0.0 35.88871 8 TTGAGGG 31475 0.0 35.528114 9 GTTTGAG 134930 0.0 34.85724 7 TGTTTGA 249570 0.0 34.020947 6 TAACTTT 22080 0.0 31.110622 1 TTGAGGC 9395 0.0 30.678839 9 AACTTTG 22835 0.0 30.210022 2 TTGAGTC 16070 0.0 27.363434 4 GTTTGAC 53315 0.0 27.351263 7 TTTGAGT 52010 0.0 26.96711 3 GTTTGAA 21200 0.0 26.607208 2 TTGAGGT 17585 0.0 25.139364 9 TTTGACC 15035 0.0 24.318361 8 TTGAGCC 18465 0.0 24.301298 9 >>END_MODULE