FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l04n01_8033.34100000035d6e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l04n01_8033.34100000035d6e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17579714
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1254050.065.3664251
TTTGTGT1359150.060.240822
ACTTTGT370600.057.099381
TTGTGTT1527250.055.1887133
TGTGTTT1603250.052.869874
GTGTTTG1770100.047.877355
TTTGAGG574750.035.888718
TTGAGGG314750.035.5281149
GTTTGAG1349300.034.857247
TGTTTGA2495700.034.0209476
TAACTTT220800.031.1106221
TTGAGGC93950.030.6788399
AACTTTG228350.030.2100222
TTGAGTC160700.027.3634344
GTTTGAC533150.027.3512637
TTTGAGT520100.026.967113
GTTTGAA212000.026.6072082
TTGAGGT175850.025.1393649
TTTGACC150350.024.3183618
TTGAGCC184650.024.3012989