FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l04n01_7965.34100000035ca3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l04n01_7965.34100000035ca3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14696702
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT323650.057.79941
CTTTGTG1023150.056.69661
TTTGTGT1050850.055.1359672
TTGTGTT1181600.050.369423
TAACTTT167550.049.9317171
AACTTTG177950.047.25172
GTGTTTG1358150.044.02255
TGTGTTT1370100.043.675984
TTGAGGG249350.031.5345089
TTTGAGG420800.030.3649858
GTTTGAG1098450.030.0773287
TTGAGGC53850.029.4507189
TTTGACG134650.028.3520728
TGTTTGA2136200.028.0868366
GTCCTCG10600.025.5331338
TTGATTC57700.025.0996974
TTTGAAT79550.024.4337833
GTTTGAT506150.024.3023192
TTTGATT197600.023.6752873
GTTTGAA173600.023.1064412
Produced by FastQC (version 0.11.2)