FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l04n01_7765.34100000035c6f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l04n01_7765.34100000035c6f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14425059
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1358450.060.2712171
TTTGTGT1432250.057.1761932
ACTTTGT462150.054.1985131
TTGTGTT1582450.053.2186853
TGTGTTT1694050.049.9799734
GTGTTTG1898300.044.625345
AACTTTG266600.041.4297752
TAACTTT266250.041.321941
TTTGAGG585350.032.804378
GTTTGAG1449200.032.027627
TGTTTGA2655750.031.9126516
TTGAGTC182050.031.4348644
TTGAGGG384700.030.2645669
TTTGACC132400.029.1208638
TTGAGGC74500.029.0632889
GTTTGAC515100.029.0095757
TTTGAGT528000.028.7612293
TGAGTGG74400.028.6550085
CTTGTGT82400.027.120952
TTTGACG178150.026.9464478