##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l04n01_7765.34100000035c6f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14425059 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.72257139468199 33.0 33.0 33.0 33.0 33.0 2 32.75655267683827 33.0 33.0 33.0 33.0 33.0 3 32.78471089788957 33.0 33.0 33.0 33.0 33.0 4 36.59607991897988 37.0 37.0 37.0 37.0 37.0 5 36.61735809884729 37.0 37.0 37.0 37.0 37.0 6 36.62597241370035 37.0 37.0 37.0 37.0 37.0 7 36.59803984163947 37.0 37.0 37.0 37.0 37.0 8 36.5524438409576 37.0 37.0 37.0 37.0 37.0 9 36.5747577878191 37.0 37.0 37.0 37.0 37.0 10-11 36.56943070388828 37.0 37.0 37.0 37.0 37.0 12-13 36.54093196429907 37.0 37.0 37.0 37.0 37.0 14-15 38.59103519091326 40.0 37.0 40.0 37.0 40.0 16-17 38.56790239124845 40.0 37.0 40.0 37.0 40.0 18-19 38.51756107202057 40.0 37.0 40.0 37.0 40.0 20-21 38.4620183182613 40.0 37.0 40.0 37.0 40.0 22-23 38.42132576372825 40.0 37.0 40.0 37.0 40.0 24-25 38.375575656224356 40.0 37.0 40.0 37.0 40.0 26-27 38.2761649363098 40.0 37.0 40.0 37.0 40.0 28-29 38.21614708820255 40.0 37.0 40.0 37.0 40.0 30-31 38.15251684585831 40.0 37.0 40.0 37.0 40.0 32-33 38.05174117485412 40.0 37.0 40.0 37.0 40.0 34-35 37.969135585511296 40.0 37.0 40.0 37.0 40.0 36-37 37.88593856011265 40.0 37.0 40.0 33.0 40.0 38-39 37.75881606446116 40.0 37.0 40.0 33.0 40.0 40-41 37.58050022533703 40.0 37.0 40.0 33.0 40.0 42-43 37.46120019335796 40.0 37.0 40.0 33.0 40.0 44-45 37.32093646896003 40.0 37.0 40.0 33.0 40.0 46-47 37.14124815711325 37.0 37.0 40.0 33.0 40.0 48-49 36.92702431927661 37.0 37.0 40.0 33.0 40.0 50-51 36.72001282629069 37.0 37.0 40.0 33.0 40.0 52-53 36.4706773123077 37.0 37.0 40.0 33.0 40.0 54-55 36.21095293267085 37.0 37.0 40.0 33.0 40.0 56-57 35.9268280982421 37.0 37.0 40.0 33.0 40.0 58-59 35.67432223327475 37.0 33.0 38.5 33.0 40.0 60-61 35.37517011195587 37.0 33.0 37.0 33.0 40.0 62-63 35.05549748531358 37.0 33.0 37.0 27.0 40.0 64-65 34.81221213722592 37.0 33.0 37.0 27.0 40.0 66-67 34.538242997827595 37.0 33.0 37.0 27.0 37.0 68-69 34.28727823574239 37.0 33.0 37.0 27.0 37.0 70-71 34.02529778214425 37.0 33.0 37.0 27.0 37.0 72-73 33.80817326293085 37.0 33.0 37.0 27.0 37.0 74-75 33.577926786989224 37.0 33.0 37.0 27.0 37.0 76-77 30.75534051541834 33.0 30.0 33.0 24.5 37.0 78-79 33.33427326016483 35.0 33.0 37.0 27.0 37.0 80-81 33.82303951061829 37.0 33.0 37.0 27.0 37.0 82-83 33.81368689722517 37.0 33.0 37.0 27.0 37.0 84-85 33.76272166373808 37.0 33.0 37.0 27.0 37.0 86-87 33.66727699345978 37.0 33.0 37.0 27.0 37.0 88-89 33.48175771759408 37.0 33.0 37.0 27.0 37.0 90-91 33.35933898086656 37.0 33.0 37.0 27.0 37.0 92-93 33.24785104865083 37.0 33.0 37.0 27.0 37.0 94-95 33.13843957241353 35.0 33.0 37.0 27.0 37.0 96-97 33.021226533631506 33.0 33.0 37.0 27.0 37.0 98-99 32.87052732331979 33.0 33.0 37.0 27.0 37.0 100-101 30.989419419359045 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14848312336781078 2306 2 0.12403787725041582 2306 3 0.09933038253996784 2306 4 -0.07796707253997681 2306 5 -0.010189324107159337 2306 6 0.05908914705101864 2306 7 0.09524120203070652 2306 8 0.16378328095262162 2306 9 0.13190846919597732 2306 10-11 0.10959345912780094 2306 12-13 0.12706400746142066 2306 14-15 0.2332581010670367 2306 16-17 0.22279427583338673 2306 18-19 0.20541392859337293 2306 20-21 0.16394689695580666 2306 22-23 0.17936790547850734 2306 24-25 0.17140575331949037 2306 26-27 0.1717190169740732 2306 28-29 0.15243803894964714 2306 30-31 0.13390842748960097 2306 32-33 0.17768953175351498 2306 34-35 0.11323444243081582 2306 36-37 0.07784679870260902 2306 38-39 0.10454078117837895 2306 40-41 0.16062093468847394 2306 42-43 0.1721400921970826 2306 44-45 0.02817522335202227 2306 46-47 0.06499360458578707 2306 48-49 0.03220142789142244 2306 50-51 0.03372817248895643 2306 52-53 -0.032841190848913016 2306 54-55 0.06897740242138184 2306 56-57 -0.004475551881803597 2306 58-59 -0.08499323011395177 2306 60-61 -0.09598831380232298 2306 62-63 -0.033306188992114016 2306 64-65 -0.05886286760044612 2306 66-67 -0.10855207982894655 2306 68-69 -0.23053756674781312 2306 70-71 -0.2401230279234312 2306 72-73 -0.13453669625191367 2306 74-75 -0.27149870475410864 2306 76-77 -0.845066268423917 2306 78-79 -0.1480354377768336 2306 80-81 -0.008867957502580737 2306 82-83 -0.1255561439824291 2306 84-85 -0.21987249883952842 2306 86-87 -0.11739136293465435 2306 88-89 -0.19372349121948673 2306 90-91 -0.28451213235529593 2306 92-93 -0.37324535927578495 2306 94-95 -0.3207542925225937 2306 96-97 -0.23434292209292096 2306 98-99 -0.14506355713246677 2306 100-101 -0.355260768040111 2307 1 0.11952412664481926 2307 2 0.10118608487017866 2307 3 0.07216529315830655 2307 4 -0.12141908557890702 2307 5 0.00569254341376535 2307 6 0.06414273098400969 2307 7 0.11772095714488273 2307 8 0.16673055689341254 2307 9 0.11456663042896764 2307 10-11 0.10313972954185857 2307 12-13 0.08708256638249878 2307 14-15 0.17502559604629653 2307 16-17 0.13841000530908332 2307 18-19 0.13782580894051222 2307 20-21 0.1347919783511884 2307 22-23 0.14204797753846066 2307 24-25 0.1289935492169363 2307 26-27 0.13935714260426124 2307 28-29 0.1626091054866592 2307 30-31 0.1459193793004303 2307 32-33 0.1193466354659165 2307 34-35 0.1683425549452906 2307 36-37 0.08343397996335256 2307 38-39 0.03457401620725875 2307 40-41 0.11329649411587184 2307 42-43 0.042556546679044516 2307 44-45 0.10965633423420229 2307 46-47 0.03398889745874101 2307 48-49 0.006327512240858368 2307 50-51 -0.04561645022074856 2307 52-53 -0.022936764153882905 2307 54-55 0.020209600558018792 2307 56-57 -0.12170677904170901 2307 58-59 -0.19595821391564527 2307 60-61 -0.20011072377712225 2307 62-63 -0.12608149451839523 2307 64-65 -0.18843833933724596 2307 66-67 -0.2481084459756957 2307 68-69 -0.36823413256174575 2307 70-71 -0.3107395634101806 2307 72-73 -0.31562099445705627 2307 74-75 -0.3165898079666718 2307 76-77 -0.865198165542008 2307 78-79 -0.2491612726914525 2307 80-81 -0.12008793286725705 2307 82-83 -0.0821893197896415 2307 84-85 -0.10528932717615902 2307 86-87 -0.21770993632903668 2307 88-89 -0.19707191507183808 2307 90-91 -0.3318855785283503 2307 92-93 -0.2973078561646858 2307 94-95 -0.1777451786549804 2307 96-97 -0.17623751881454552 2307 98-99 -0.225679378202166 2307 100-101 -0.6585123250133371 2201 1 0.09812757865223176 2201 2 0.06310070507752386 2201 3 0.056007603722100896 2201 4 -0.1529119472261513 2201 5 -0.02918735896525959 2201 6 0.03364596741981529 2201 7 0.06833631446465915 2201 8 0.10199370020252019 2201 9 0.0455742940426731 2201 10-11 0.06661665654166171 2201 12-13 0.06586012639139938 2201 14-15 0.14018547418274352 2201 16-17 0.11359509672406887 2201 18-19 0.13842532182970047 2201 20-21 0.1373426379810354 2201 22-23 0.054950110918738915 2201 24-25 0.10145564269696905 2201 26-27 0.14458815048626406 2201 28-29 0.10714034830903785 2201 30-31 0.06786268440347243 2201 32-33 0.1674846701269601 2201 34-35 0.10244872347430345 2201 36-37 0.031749353263109015 2201 38-39 0.07865366263547457 2201 40-41 0.15785264447349334 2201 42-43 0.1145737535499265 2201 44-45 0.09927730969988602 2201 46-47 0.015225386579928113 2201 48-49 0.024593568997346438 2201 50-51 0.034889399039968794 2201 52-53 -0.008952382364554978 2201 54-55 0.0033013979475171595 2201 56-57 -0.03326595761341622 2201 58-59 -0.04707215871647463 2201 60-61 -0.05657428000534992 2201 62-63 0.06006085277977036 2201 64-65 -0.013089999079006986 2201 66-67 -0.1113793614900942 2201 68-69 -0.16318148371785668 2201 70-71 -0.1997724913600436 2201 72-73 -0.08986612481741219 2201 74-75 -0.214033730053643 2201 76-77 -0.8288935860346704 2201 78-79 -0.09854782814989704 2201 80-81 0.04812414814959709 2201 82-83 0.10895124877539075 2201 84-85 0.008431471515685018 2201 86-87 0.02739640886839112 2201 88-89 0.12117756678365765 2201 90-91 -0.01721475410436568 2201 92-93 -0.10213386714703887 2201 94-95 -0.07728343851177755 2201 96-97 -0.02238207710359319 2201 98-99 -0.09456881615713542 2201 100-101 -0.5255725596042886 1101 1 0.08279401112999807 1101 2 0.0793746230639698 1101 3 0.07003367864847831 1101 4 -0.1430235862796394 1101 5 -0.02706739954247439 1101 6 0.04710041360056749 1101 7 0.08894301143795502 1101 8 0.1613194695976503 1101 9 0.06611120961822792 1101 10-11 0.05875561504031879 1101 12-13 0.05250558558920204 1101 14-15 0.09871636538149176 1101 16-17 0.08573006501841007 1101 18-19 0.10501524107367288 1101 20-21 0.07669520164363064 1101 22-23 0.09377003227767489 1101 24-25 0.09274051644673875 1101 26-27 0.11635390431477077 1101 28-29 0.09730316667792493 1101 30-31 0.1331363010103246 1101 32-33 0.14064758257213583 1101 34-35 0.12413700415543616 1101 36-37 0.06927236036607809 1101 38-39 0.07930674145308814 1101 40-41 0.1410146392206002 1101 42-43 0.12867304163149385 1101 44-45 0.12565691812727664 1101 46-47 0.12168476484612967 1101 48-49 0.1075840981855265 1101 50-51 0.01052154833078589 1101 52-53 -0.007896044090287546 1101 54-55 0.051707011128478086 1101 56-57 0.08496145859194826 1101 58-59 -0.022010215844126435 1101 60-61 -0.04681834575586663 1101 62-63 -0.005512598781209022 1101 64-65 -0.06352727207453768 1101 66-67 -0.07514284756295808 1101 68-69 -0.10747542862997506 1101 70-71 -0.22852683050629707 1101 72-73 -0.07941219836269653 1101 74-75 -0.19287323759155584 1101 76-77 -0.7040617701065948 1101 78-79 -0.001174660243584924 1101 80-81 0.030709845283517723 1101 82-83 0.09308724757546827 1101 84-85 -0.024492756957442907 1101 86-87 0.05383452142136491 1101 88-89 0.06667506956854652 1101 90-91 0.025872550643661896 1101 92-93 -0.13084132541135318 1101 94-95 -0.06975358257150788 1101 96-97 0.05519030816631698 1101 98-99 -0.02673874644143126 1101 100-101 -0.3547749073457389 2304 1 0.08561756961154998 2304 2 0.05648389102245943 2304 3 0.053063384114267365 2304 4 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18-19 0.0727447985854397 2202 20-21 0.03352427638157707 2202 22-23 0.02814012799087351 2202 24-25 0.05666173557739285 2202 26-27 0.08712905102138535 2202 28-29 0.058240031099209943 2202 30-31 0.06485080793063247 2202 32-33 0.05698387054418674 2202 34-35 0.04520846709874604 2202 36-37 0.04419209874748731 2202 38-39 0.061483171382320734 2202 40-41 0.075505255435246 2202 42-43 0.08250858852834853 2202 44-45 0.0057634297897593 2202 46-47 0.06793041001677835 2202 48-49 0.05110902313880672 2202 50-51 0.015157723536816547 2202 52-53 0.007324808579241449 2202 54-55 -0.03535901326425517 2202 56-57 0.03363156904345743 2202 58-59 0.034373456279155334 2202 60-61 -0.050014207924718335 2202 62-63 0.006413413847141669 2202 64-65 0.048204909434545584 2202 66-67 0.030718989398046403 2202 68-69 -0.02488976756155381 2202 70-71 -0.06429302870252229 2202 72-73 0.0030790604528121435 2202 74-75 -0.07045065772860681 2202 76-77 -0.08728373125822486 2202 78-79 0.10727715244168223 2202 80-81 0.07565759959140195 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1213 54-55 -0.20516175953630267 1213 56-57 -0.15286167098670234 1213 58-59 -0.2279428476748393 1213 60-61 -0.23613799116400003 1213 62-63 -0.10586897787162997 1213 64-65 -0.10254589582701357 1213 66-67 -0.05143521978327925 1213 68-69 0.027195895066455478 1213 70-71 -0.10438456665826834 1213 72-73 -0.03606352420922576 1213 74-75 0.04964842955198634 1213 76-77 0.4850091542192452 1213 78-79 -0.006736201350811655 1213 80-81 -0.14151023790727635 1213 82-83 -0.21878452225420375 1213 84-85 -0.1557142354673644 1213 86-87 -0.1348967405859156 1213 88-89 -0.03788052266639852 1213 90-91 -0.052946855080172384 1213 92-93 0.1091550956889833 1213 94-95 0.04342833518988698 1213 96-97 0.08709637499772072 1213 98-99 0.1544369146471709 1213 100-101 0.42642718802585833 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 39.0 8 430.0 9 1334.0 10 879.0 11 316.0 12 325.0 13 569.0 14 1335.0 15 2732.0 16 5055.0 17 7710.0 18 10820.0 19 14520.0 20 19117.0 21 24878.0 22 32253.0 23 40385.0 24 51927.0 25 66412.0 26 84657.0 27 107147.0 28 136726.0 29 177707.0 30 230346.0 31 304929.0 32 418290.0 33 603620.0 34 956999.0 35 1790392.0 36 3801294.0 37 4817300.0 38 714588.0 39 27.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 20.28883503047045 22.835439579718315 31.06476899382095 25.810956395990285 2 26.72377977795446 22.67626080420191 30.188327132665453 20.411632285178175 3 21.51662603251744 20.915976842798358 37.16384106297243 20.403556061711775 4 21.418435157962406 20.796116535919655 36.25769385966578 21.527754446452153 5 28.09082444654126 20.11625741010834 31.957962875576452 19.83495526777395 6 29.266902825146158 20.608927838700694 30.780976355105377 19.34319298104777 7 28.02151450472404 25.721024780557222 26.48108406350366 19.776376651215084 8 26.968097669479203 22.0271750708264 30.368569029769652 20.636158229924746 9 22.85975398783464 21.221195698402344 34.57057610648248 21.34847420728054 10-11 27.314953045239687 22.020207201982938 28.912870696160258 21.751969056617114 12-13 26.6185907501296 25.77300394720405 25.23681629826881 22.37158900439754 14-15 26.415495175132055 23.6410349268613 26.550756329714588 23.392713568292063 16-17 25.95673958768557 23.42286780248178 26.060642108985483 24.559750500847173 18-19 25.631215789134725 24.01309415788178 26.718039766769756 23.637650286213734 20-21 24.30099778948767 24.713966678466832 27.186137006564987 23.79889852548051 22-23 24.462995946449247 24.697862375461504 26.711875193132485 24.127266484956767 24-25 24.733070415864503 24.6071090593113 26.471337136298718 24.18848338852548 26-27 24.565091900144047 24.5611439093594 26.76107251970339 24.112691670793165 28-29 24.803555396203233 24.75668557057548 26.112995447713594 24.326763585507692 30-31 24.87323275419532 24.56030162510947 26.118028356071193 24.448437264624012 32-33 24.69765634927386 25.000875213058055 25.933169493448865 24.368298944219223 34-35 24.537778320352103 25.134216088821542 25.774820054462168 24.553185536364182 36-37 24.558784533948756 25.161855435326284 25.992600914192305 24.286759116532654 38-39 24.76904595474461 24.93304661267458 25.83382831182206 24.464079120758747 40-41 24.496799923544668 25.55696249789025 25.604715882440843 24.341521696124236 42-43 24.445519902302028 25.385241067090824 25.809855122377584 24.359383908229567 44-45 24.71366321621284 24.991107488711137 26.127913930889296 24.167315364186727 46-47 24.248840160723088 25.292267435439953 26.122517072547158 24.336375331289805 48-49 24.471592190990727 25.013139463266576 26.142015457617095 24.373252888125602 50-51 24.26497448975436 25.229609195300306 26.4403913499378 24.065024965007535 52-53 24.339473911455023 25.48892784803663 26.21635490816796 23.955243332340384 54-55 24.190960631413773 25.237585610428344 26.06136548015729 24.510088278000598 56-57 24.29956261900029 25.497869529470336 26.138445815526257 24.06412203600311 58-59 24.12822990557821 25.453837320657097 26.360965970889144 24.056966802875554 60-61 24.55094019337918 25.352272466907465 25.679566018359317 24.417221321354038 62-63 24.250098405368558 25.61988449795515 26.026344770539755 24.103672326136536 64-65 24.45759305253236 25.681450722287224 25.901040958241182 23.95991526693923 66-67 24.791597133179685 25.820899578784797 25.474589881267423 23.91291340676809 68-69 24.749863709207016 25.728581544363195 25.7144090353212 23.80714571110859 70-71 24.517624032587957 26.222000684191478 25.471556977315966 23.788818305904595 72-73 24.783707442136393 25.74641022789329 25.502178422344628 23.967703907625683 74-75 24.627404477340583 25.694098366167754 25.57523481279883 24.103262343692833 76-77 24.75320070864316 25.449395051922423 25.501128193636635 24.296276045797786 78-79 24.91382216788976 25.287488804213382 25.427682688649917 24.371006339246946 80-81 24.731700739412734 25.263792971367383 25.577680280166398 24.42682600905348 82-83 24.37868580768973 25.72169142324085 25.598089955580004 24.301532813489413 84-85 24.430956688289655 25.658173177302658 25.528739619498342 24.38213051490935 86-87 24.140749953476657 25.719680024805054 25.74386730849925 24.395702713219038 88-89 24.143703682852593 26.377597031015686 25.221356159073448 24.257343127058263 90-91 24.197949261220263 25.87041597930143 25.418010928799568 24.51362383067874 92-93 23.974505222902327 26.25610729890382 25.305871943993218 24.46351553420063 94-95 24.120344286929996 26.572895010124743 24.933450441881856 24.37331026106341 96-97 24.039430509209698 26.1213725712932 25.11274518453036 24.72645173496674 98-99 24.346139325058697 26.00849312469011 25.23371969970701 24.411647850544192 100-101 24.839001528677613 26.37381231759702 24.512645067659655 24.27454108606571 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 17780.0 1 10405.0 2 4824.5 3 5871.0 4 5571.5 5 4481.0 6 2667.0 7 2484.5 8 2763.5 9 2933.5 10 2811.5 11 2489.0 12 2188.5 13 2036.5 14 1943.0 15 1965.0 16 2062.5 17 2155.0 18 2309.5 19 2448.5 20 2613.0 21 2920.5 22 3247.5 23 3586.5 24 3955.5 25 4432.0 26 5026.5 27 5712.0 28 6474.5 29 7364.0 30 9118.5 31 12554.5 32 26871.0 33 48481.0 34 70777.5 35 110241.5 36 147207.5 37 204924.0 38 276879.0 39 292776.5 40 280660.5 41 290439.0 42 332309.5 43 403344.0 44 492453.5 45 598184.5 46 703229.5 47 796193.5 48 903743.5 49 990283.5 50 976886.5 51 901567.5 52 843637.0 53 819181.5 54 766116.0 55 672011.0 56 586442.5 57 472739.5 58 364525.5 59 292416.0 60 222626.0 61 150642.0 62 95180.5 63 60338.5 64 31973.5 65 16512.0 66 9668.5 67 6266.0 68 4810.5 69 3734.0 70 2953.5 71 2430.0 72 2178.0 73 1935.5 74 1638.5 75 1282.5 76 1009.5 77 902.0 78 829.5 79 721.5 80 551.5 81 386.0 82 269.5 83 240.0 84 192.5 85 121.5 86 96.0 87 101.0 88 103.5 89 96.5 90 79.5 91 63.0 92 57.0 93 63.0 94 46.5 95 35.5 96 38.5 97 29.0 98 18.0 99 7.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12582270894004663 2 0.0 3 0.0 4 0.33845268847773863 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.772952263141523E-5 12-13 0.0011438428085458784 14-15 6.932380657853808E-6 16-17 0.0 18-19 0.0 20-21 8.492166305870915E-4 22-23 3.5701760387947117E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.002107443719987558 38-39 0.007150750648576204 40-41 0.006589227815290045 42-43 0.0013518142282814927 44-45 0.0 46-47 0.0 48-49 1.733095164463452E-5 50-51 0.0011681061408483667 52-53 0.011705324740786154 54-55 0.009452301026983667 56-57 0.00866547582231726 58-59 0.012107402818941676 60-61 0.002727891788865474 62-63 2.287685617091757E-4 64-65 0.0032235570059020212 66-67 0.010765987161646964 68-69 0.01921309299324183 70-71 0.025355182256100305 72-73 0.018682765872916014 74-75 0.003719222222938568 76-77 0.013885558457681177 78-79 0.007667213007586313 80-81 0.005365662629178848 82-83 0.003279016051164851 84-85 0.009999959098954118 86-87 0.015188846021357694 88-89 0.012547608990715392 90-91 0.030180119193966553 92-93 0.01350427752149922 94-95 0.013677587037945563 96-97 0.007282465881075426 98-99 0.012100470438283823 100-101 0.1372472722641897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4425059E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.430446483077667 #Duplication Level Percentage of deduplicated Percentage of total 1 76.77257291049226 13.381802234845136 2 9.340946749041848 3.256337448209045 3 3.384034854319818 1.7695571527527334 4 1.8434639822331136 1.285296011431821 5 1.2940562233401824 1.1277988873512328 6 0.8198206089536255 0.8573903550054188 7 0.7584127544251209 0.9253631049663418 8 0.5342900378087463 0.7450331128373516 9 0.40308992583217806 0.6323433642076973 >10 3.214374711782728 11.66947138608159 >50 0.6282513198394697 7.809531488392503 >100 0.8682348340008497 33.298738195835355 >500 0.10075148104627404 11.746678984787271 >1k 0.03725339265048249 10.899321944240109 >5k 3.667934848501141E-4 0.4118308623014547 >10k+ 7.94207487968406E-5 0.18350546675474866 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.545904526283047E-5 0.0 0.0 2 5.545904526283047E-5 0.0 0.0 3 6.239142592068428E-5 0.0 0.0 4 6.239142592068428E-5 0.0 0.0 5 6.239142592068428E-5 0.0 0.0 6 6.932380657853809E-5 0.0 0.0 7 8.31885678942457E-5 0.0 0.0 8 8.31885678942457E-5 0.0 0.0 9 9.705332920995332E-5 0.0 0.0 10-11 1.3864761315707615E-4 0.0 0.0 12-13 2.495657036827371E-4 0.0 0.0 14-15 4.2980760078693614E-4 0.0 0.0 16-17 6.100494978911351E-4 0.0 0.0 18-19 8.388180596003109E-4 0.0 0.0 20-21 0.0011022485245987556 0.0 0.0 22-23 0.001476597080122861 0.0 0.0 24-25 0.0018925399195940896 0.0 0.0 26-27 0.0023708741849860025 0.0 0.0 28-29 0.0028804041633382575 0.0 0.0 30-31 0.0033864679513615856 0.0 0.0 32-33 0.003892531739384914 0.0 3.4661903289269044E-6 34-35 0.004603100756814929 0.0 6.932380657853809E-6 36-37 0.005473114529375582 0.0 6.932380657853809E-6 38-39 0.006731341618776048 0.0 6.932380657853809E-6 40-41 0.008201006318241055 0.0 6.932380657853809E-6 42-43 0.009913304340730945 0.0 6.932380657853809E-6 44-45 0.011878634257232502 0.0 6.932380657853809E-6 46-47 0.014596127475111194 0.0 6.932380657853809E-6 48-49 0.01778155638739502 0.0 1.0398570986780712E-5 50-51 0.021764209075332032 0.0 1.3864761315707617E-5 52-53 0.027181864559444782 0.0 1.3864761315707617E-5 54-55 0.03327889334802721 0.0 1.3864761315707617E-5 56-57 0.040918376832982106 0.0 1.3864761315707617E-5 58-59 0.05003099120773094 0.0 1.3864761315707617E-5 60-61 0.061278778825098745 0.0 2.0797141973561424E-5 62-63 0.07506381776324103 0.0 2.0797141973561424E-5 64-65 0.09158368087090665 0.0 2.0797141973561424E-5 66-67 0.11190248857907617 0.0 2.0797141973561424E-5 68-69 0.1367412084761664 0.0 2.0797141973561424E-5 70-71 0.16811023095295485 0.0 2.0797141973561424E-5 72-73 0.2052851222306959 0.0 2.0797141973561424E-5 74-75 0.24957263606339497 0.0 2.0797141973561424E-5 76-77 0.30307328379038173 0.0 2.0797141973561424E-5 78-79 0.3684906938682192 0.0 2.0797141973561424E-5 80-81 0.4455614358319089 0.0 2.0797141973561424E-5 82-83 0.5380463261883366 0.0 2.0797141973561424E-5 84-85 0.6467668520454579 0.0 2.0797141973561424E-5 86-87 0.7737299376037214 6.932380657853809E-6 2.0797141973561424E-5 88-89 0.9254173587782206 6.932380657853809E-6 2.0797141973561424E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 135845 0.0 60.271217 1 TTTGTGT 143225 0.0 57.176193 2 ACTTTGT 46215 0.0 54.198513 1 TTGTGTT 158245 0.0 53.218685 3 TGTGTTT 169405 0.0 49.979973 4 GTGTTTG 189830 0.0 44.62534 5 AACTTTG 26660 0.0 41.429775 2 TAACTTT 26625 0.0 41.32194 1 TTTGAGG 58535 0.0 32.80437 8 GTTTGAG 144920 0.0 32.02762 7 TGTTTGA 265575 0.0 31.912651 6 TTGAGTC 18205 0.0 31.434864 4 TTGAGGG 38470 0.0 30.264566 9 TTTGACC 13240 0.0 29.120863 8 TTGAGGC 7450 0.0 29.063288 9 GTTTGAC 51510 0.0 29.009575 7 TTTGAGT 52800 0.0 28.761229 3 TGAGTGG 7440 0.0 28.655008 5 CTTGTGT 8240 0.0 27.12095 2 TTTGACG 17815 0.0 26.946447 8 >>END_MODULE