##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l04n01_7331.34100000035c1f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19885260 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.72489839207534 33.0 33.0 33.0 33.0 33.0 2 32.75815699669001 33.0 33.0 33.0 33.0 33.0 3 32.7861284187383 33.0 33.0 33.0 33.0 33.0 4 36.59684545236019 37.0 37.0 37.0 37.0 37.0 5 36.62100344677415 37.0 37.0 37.0 37.0 37.0 6 36.628023923247675 37.0 37.0 37.0 37.0 37.0 7 36.605343908000194 37.0 37.0 37.0 37.0 37.0 8 36.56029838181648 37.0 37.0 37.0 37.0 37.0 9 36.58304060394483 37.0 37.0 37.0 37.0 37.0 10-11 36.57806221794435 37.0 37.0 37.0 37.0 37.0 12-13 36.55578126712952 37.0 37.0 37.0 37.0 37.0 14-15 38.626157817398415 40.0 37.0 40.0 37.0 40.0 16-17 38.604121922469204 40.0 37.0 40.0 37.0 40.0 18-19 38.56844351041927 40.0 37.0 40.0 37.0 40.0 20-21 38.50811606184681 40.0 37.0 40.0 37.0 40.0 22-23 38.45666501217485 40.0 37.0 40.0 37.0 40.0 24-25 38.41277675021599 40.0 37.0 40.0 37.0 40.0 26-27 38.313124419796374 40.0 37.0 40.0 37.0 40.0 28-29 38.261118009017736 40.0 37.0 40.0 37.0 40.0 30-31 38.19995034010116 40.0 37.0 40.0 37.0 40.0 32-33 38.09808860935185 40.0 37.0 40.0 37.0 40.0 34-35 38.01755262440622 40.0 37.0 40.0 37.0 40.0 36-37 37.9346614276102 40.0 37.0 40.0 35.0 40.0 38-39 37.80908308465668 40.0 37.0 40.0 33.0 40.0 40-41 37.64195773653449 40.0 37.0 40.0 33.0 40.0 42-43 37.526149896958856 40.0 37.0 40.0 33.0 40.0 44-45 37.391840514028985 40.0 37.0 40.0 33.0 40.0 46-47 37.21096603715516 37.0 37.0 40.0 33.0 40.0 48-49 36.99552517794588 37.0 37.0 40.0 33.0 40.0 50-51 36.77914279219884 37.0 37.0 40.0 33.0 40.0 52-53 36.52121604142968 37.0 37.0 40.0 33.0 40.0 54-55 36.245234108078044 37.0 37.0 40.0 33.0 40.0 56-57 35.9480310541577 37.0 37.0 40.0 33.0 40.0 58-59 35.6911366509666 37.0 33.0 37.0 33.0 40.0 60-61 35.394052076764396 37.0 33.0 37.0 33.0 40.0 62-63 35.079748441810665 37.0 33.0 37.0 30.0 40.0 64-65 34.852921711860944 37.0 33.0 37.0 27.0 40.0 66-67 34.58868372855069 37.0 33.0 37.0 27.0 37.0 68-69 34.34819215338396 37.0 33.0 37.0 27.0 37.0 70-71 34.09875088382047 37.0 33.0 37.0 27.0 37.0 72-73 33.88932639050231 37.0 33.0 37.0 27.0 37.0 74-75 33.67316426840786 37.0 33.0 37.0 27.0 37.0 76-77 30.839251385196874 33.0 30.0 33.0 24.5 37.0 78-79 33.45326586124597 35.0 33.0 37.0 27.0 37.0 80-81 33.94556973356144 37.0 33.0 37.0 27.0 37.0 82-83 33.94810668806946 37.0 33.0 37.0 27.0 37.0 84-85 33.89008127125318 37.0 33.0 37.0 27.0 37.0 86-87 33.785827693477486 37.0 33.0 37.0 27.0 37.0 88-89 33.597860048095924 37.0 33.0 37.0 27.0 37.0 90-91 33.466969403467694 37.0 33.0 37.0 27.0 37.0 92-93 33.35973510027025 37.0 33.0 37.0 27.0 37.0 94-95 33.254000727171785 35.0 33.0 37.0 27.0 37.0 96-97 33.14117388960466 33.0 33.0 37.0 27.0 37.0 98-99 32.99629190163971 33.0 33.0 37.0 27.0 37.0 100-101 31.056456742330752 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14870225207764065 2306 2 0.10774931542060528 2306 3 0.09516025076190004 2306 4 -0.12312803436408615 2306 5 -0.011004498546896002 2306 6 0.0773204461440784 2306 7 0.1152059998780075 2306 8 0.17370311935949445 2306 9 0.11743619961170992 2306 10-11 0.12009715305828195 2306 12-13 0.1375038291235242 2306 14-15 0.23837644255161905 2306 16-17 0.2372085572965048 2306 18-19 0.18708501925878807 2306 20-21 0.15589389149351973 2306 22-23 0.18172052204823075 2306 24-25 0.17500397731334516 2306 26-27 0.20915628108228645 2306 28-29 0.1856729954364411 2306 30-31 0.19159008791771015 2306 32-33 0.19831148221119577 2306 34-35 0.20176609572926907 2306 36-37 0.15360854005716362 2306 38-39 0.1198814884303161 2306 40-41 0.2088735920333562 2306 42-43 0.13764841300858421 2306 44-45 0.07618920732208778 2306 46-47 0.0510656647864991 2306 48-49 0.08683219278555754 2306 50-51 0.019357693390908537 2306 52-53 0.07818450644162311 2306 54-55 0.053000497222249976 2306 56-57 0.03916938505604861 2306 58-59 -0.03295875651740232 2306 60-61 -0.10699867121821427 2306 62-63 0.012485581719744232 2306 64-65 -0.027575821539940648 2306 66-67 -0.09228856862981871 2306 68-69 -0.21876773254806636 2306 70-71 -0.1546783108612928 2306 72-73 -0.10851183267815401 2306 74-75 -0.24838911653503715 2306 76-77 -0.8157468417279432 2306 78-79 -0.13398227779652672 2306 80-81 0.06434972881420009 2306 82-83 -0.0786788826430822 2306 84-85 -0.1598790436385471 2306 86-87 -0.12143104371121893 2306 88-89 -0.190174085218203 2306 90-91 -0.2703300193488545 2306 92-93 -0.3511278704455876 2306 94-95 -0.3659207403515765 2306 96-97 -0.3197047351473472 2306 98-99 -0.1643038845394429 2306 100-101 -0.4901354341735953 2307 1 0.12927367697565018 2307 2 0.10022533045684412 2307 3 0.08084436976682241 2307 4 -0.06767353209080795 2307 5 0.03520001386017668 2307 6 0.10074725626844838 2307 7 0.12542529888332155 2307 8 0.18758264874403352 2307 9 0.12502335341743276 2307 10-11 0.11047778394671326 2307 12-13 0.08444105991934947 2307 14-15 0.19483759277632373 2307 16-17 0.14438875197618017 2307 18-19 0.10874515981771538 2307 20-21 0.15960917028194643 2307 22-23 0.12884192461616095 2307 24-25 0.16014513891647653 2307 26-27 0.15190201144891802 2307 28-29 0.13211907174511595 2307 30-31 0.1400068669192649 2307 32-33 0.14397852375406472 2307 34-35 0.1641893132206178 2307 36-37 0.114327926472086 2307 38-39 0.07996378470834742 2307 40-41 0.10256218991005994 2307 42-43 0.0338804563166093 2307 44-45 0.12381133122015342 2307 46-47 0.07182699084825828 2307 48-49 0.03328892520912774 2307 50-51 0.007938128353252694 2307 52-53 -0.04253519961211083 2307 54-55 0.03171316890087894 2307 56-57 -0.0720593520438868 2307 58-59 -0.12185824547031388 2307 60-61 -0.10050727137703319 2307 62-63 -0.08858282437724085 2307 64-65 -0.20980381472073617 2307 66-67 -0.20495873834730816 2307 68-69 -0.3284960600996243 2307 70-71 -0.2774568658117502 2307 72-73 -0.3089472021582935 2307 74-75 -0.35224082662942635 2307 76-77 -0.9043148973829531 2307 78-79 -0.28201826251886786 2307 80-81 -0.12657482374950035 2307 82-83 -0.13990737867464986 2307 84-85 -0.14310157331718898 2307 86-87 -0.16386869316244912 2307 88-89 -0.2022120308085391 2307 90-91 -0.31890618918670555 2307 92-93 -0.2304514540215834 2307 94-95 -0.20310236715594954 2307 96-97 -0.17033674945573551 2307 98-99 -0.20226326934010075 2307 100-101 -0.6341782820232034 2201 1 0.09388495323467083 2201 2 0.07696542637718551 2201 3 0.06761333235522926 2201 4 -0.12887242627952844 2201 5 -0.022654733851958042 2201 6 0.023887015850668547 2201 7 0.07396292806098614 2201 8 0.07624785459429972 2201 9 0.07646123420476414 2201 10-11 0.07852932851182715 2201 12-13 0.0812890013127614 2201 14-15 0.15759964290175077 2201 16-17 0.10127628870863248 2201 18-19 0.0823440712055401 2201 20-21 0.13250331288457318 2201 22-23 0.05364970692198057 2201 24-25 0.10640030306306159 2201 26-27 0.17091424462645932 2201 28-29 0.1229298040719442 2201 30-31 0.10318877885774924 2201 32-33 0.14290506465813735 2201 34-35 0.09238458251026316 2201 36-37 0.05394302994820066 2201 38-39 0.09987757936968222 2201 40-41 0.1269591253944924 2201 42-43 0.11079207595541618 2201 44-45 0.06007368866541185 2201 46-47 -0.011093733516709392 2201 48-49 -0.010274422659669824 2201 50-51 0.01796261215670114 2201 52-53 -0.04955921791793827 2201 54-55 -0.05762298912984676 2201 56-57 -0.05616418725752936 2201 58-59 -0.05975890109284876 2201 60-61 -0.10371423003665114 2201 62-63 0.05092579210610637 2201 64-65 -0.06493257642854644 2201 66-67 -0.1851735319486565 2201 68-69 -0.24444710121692026 2201 70-71 -0.2092495737636213 2201 72-73 -0.15232785104243618 2201 74-75 -0.2276451251187126 2201 76-77 -0.8720822516054483 2201 78-79 -0.10083167774045165 2201 80-81 -0.021595681823008306 2201 82-83 0.027687080885939963 2201 84-85 -0.06688675645133912 2201 86-87 -0.04527667491976217 2201 88-89 -0.04148401414617808 2201 90-91 -0.08460894413908449 2201 92-93 -0.17260316636109252 2201 94-95 -0.18983376381703465 2201 96-97 -0.06918429493730827 2201 98-99 -0.13101512089269107 2201 100-101 -0.5181137537928819 1101 1 0.08349291241077594 1101 2 0.0714280703914838 1101 3 0.054536245123877336 1101 4 -0.16837342576724978 1101 5 -0.032023545878153925 1101 6 0.06241380532937768 1101 7 0.07696830610591121 1101 8 0.15225448288725119 1101 9 0.062377686750373584 1101 10-11 0.06958475374117512 1101 12-13 0.06316895253215904 1101 14-15 0.13268487355225744 1101 16-17 0.1328469048644365 1101 18-19 0.13642301258410328 1101 20-21 0.11191127271375478 1101 22-23 0.13038046680732407 1101 24-25 0.15760222378516175 1101 26-27 0.17140841817102626 1101 28-29 0.13253557545806416 1101 30-31 0.1280358784609703 1101 32-33 0.1703521288802392 1101 34-35 0.15953806606160015 1101 36-37 0.1698134966466398 1101 38-39 0.1672138077311658 1101 40-41 0.20859988912719984 1101 42-43 0.1492239587590234 1101 44-45 0.1399011317727883 1101 46-47 0.1551690947790476 1101 48-49 0.1054207903444464 1101 50-51 0.06069036176595688 1101 52-53 0.04039285238636836 1101 54-55 0.05214104648407414 1101 56-57 0.09438785035478503 1101 58-59 0.017130468764094076 1101 60-61 0.03416096682122571 1101 62-63 0.01641284813024413 1101 64-65 -0.07135361516971273 1101 66-67 -0.09186463501930575 1101 68-69 -0.07126992499495088 1101 70-71 -0.14948477943412541 1101 72-73 -0.013165161870155373 1101 74-75 -0.1101813268963383 1101 76-77 -0.6326798467267736 1101 78-79 0.03597027271560904 1101 80-81 0.10423927506563047 1101 82-83 0.06985210363684757 1101 84-85 0.0768472901130508 1101 86-87 0.1429917478949463 1101 88-89 0.12332027469050644 1101 90-91 0.05445425725265096 1101 92-93 -0.03436768524802147 1101 94-95 0.020442760159241402 1101 96-97 0.02933865870593877 1101 98-99 -0.01682698593658216 1101 100-101 -0.20986389357902624 2304 1 0.11354642510989521 2304 2 0.07782860780392298 2304 3 0.07267564843276375 2304 4 -0.1679249617675893 2304 5 -0.04484688080847121 2304 6 0.07878200190776141 2304 7 0.06529134537871784 2304 8 0.15120390638519154 2304 9 0.09726192535747202 2304 10-11 0.06595171844277559 2304 12-13 0.0529966220979432 2304 14-15 0.051932693551357545 2304 16-17 0.10453100000494686 2304 18-19 0.08570562301945017 2304 20-21 0.08981012961559287 2304 22-23 0.07064313180611492 2304 24-25 0.06640147506446681 2304 26-27 0.10996871931784824 2304 28-29 0.09058578763310265 2304 30-31 0.05500994331543296 2304 32-33 0.10346192716873759 2304 34-35 0.13448439168307402 2304 36-37 0.12750891627969452 2304 38-39 0.06622634634052105 2304 40-41 0.1488571659917568 2304 42-43 0.15469598938938134 2304 44-45 0.08438965773562757 2304 46-47 0.1152212057849411 2304 48-49 0.03502282126419942 2304 50-51 -0.047037503421293536 2304 52-53 -0.026414064973231177 2304 54-55 0.05372465911238322 2304 56-57 -0.029148878500784292 2304 58-59 -0.08386656249069802 2304 60-61 -0.03105644386434392 2304 62-63 0.03209641910400052 2304 64-65 -0.00842995763768073 2304 66-67 -0.030585738186545086 2304 68-69 -0.09728739259733743 2304 70-71 -0.07441324602631738 2304 72-73 -0.043326684902886825 2304 74-75 -0.11362470906816924 2304 76-77 -0.2831280772288203 2304 78-79 0.07489103402820518 2304 80-81 0.13027498024644757 2304 82-83 0.05160120168157789 2304 84-85 0.031138760367547036 2304 86-87 0.11802301004080107 2304 88-89 0.05774888162891045 2304 90-91 -0.009145768559449152 2304 92-93 -0.06601364103050855 2304 94-95 -0.006165732346943287 2304 96-97 0.024585804250733645 2304 98-99 0.003612425431505528 2304 100-101 -0.22600536751641442 2202 1 0.08722366418003702 2202 2 0.06137445260345942 2202 3 0.014111115224359594 2202 4 -0.19475127153734917 2202 5 -0.06503676491173138 2202 6 0.0318114252430064 2202 7 0.021066602231421427 2202 8 0.13548175960148967 2202 9 0.055572312195266704 2202 10-11 0.06582180343842481 2202 12-13 0.037444873051541094 2202 14-15 0.028148994559607843 2202 16-17 0.043527107718780655 2202 18-19 0.03844232719731622 2202 20-21 0.041412473539288897 2202 22-23 0.03577474118731061 2202 24-25 0.04343924788884124 2202 26-27 0.06603170010927784 2202 28-29 0.040174545649918514 2202 30-31 0.028007773670303493 2202 32-33 0.01522133243148005 2202 34-35 0.019229546375044038 2202 36-37 0.052881499792292175 2202 38-39 0.05237083691121569 2202 40-41 0.09222734371269325 2202 42-43 0.07113240361782402 2202 44-45 0.04458633677796797 2202 46-47 0.06690568984549827 2202 48-49 0.06524061581170315 2202 50-51 0.04651992733653998 2202 52-53 -0.002507356469628519 2202 54-55 -0.0074722003214091615 2202 56-57 0.06653989851850639 2202 58-59 0.052759749930508804 2202 60-61 -0.06582053451439407 2202 62-63 0.05045364611851255 2202 64-65 0.004794579822593903 2202 66-67 0.01615193087949507 2202 68-69 -0.05160417175532217 2202 70-71 -0.1419121234373435 2202 72-73 -0.040404342737822674 2202 74-75 -0.10521255935431384 2202 76-77 -0.12442398295232948 2202 78-79 -0.0057587091500650445 2202 80-81 0.10631430077273052 2202 82-83 0.10865625648798982 2202 84-85 0.08107735046998243 2202 86-87 0.05921965000608509 2202 88-89 0.10873325327558803 2202 90-91 0.06510223084483613 2202 92-93 0.07583529781696541 2202 94-95 0.03020549003493045 2202 96-97 0.06821200912646219 2202 98-99 0.04084879379765738 2202 100-101 -0.009190629943113038 1102 1 0.0644440806584825 1102 2 0.024251052226148317 1102 3 0.028287602519903032 1102 4 -0.21965408811573184 1102 5 -0.06767931789169523 1102 6 0.006735895434005101 1102 7 0.0652688119717979 1102 8 0.12524754137128724 1102 9 0.05072204883452258 1102 10-11 0.04558166910220507 1102 12-13 0.031301940191447386 1102 14-15 0.052478506225348553 1102 16-17 0.05044022774050205 1102 18-19 0.026500783362919833 1102 20-21 -0.004232824664690327 1102 22-23 0.018458956694807682 1102 24-25 0.061677499641163536 1102 26-27 0.10458197136183855 1102 28-29 0.09203892584180551 1102 30-31 0.05303753017594204 1102 32-33 0.07951496131360614 1102 34-35 0.08902953978149242 1102 36-37 0.04726234298499321 1102 38-39 -0.007523481366398244 1102 40-41 0.06359775317056915 1102 42-43 0.04589240555291951 1102 44-45 0.010876020019807697 1102 46-47 -0.008093735321338613 1102 48-49 -0.024650616675565118 1102 50-51 -0.0373611831119689 1102 52-53 -0.03173418792272997 1102 54-55 -0.03028583351555625 1102 56-57 -0.003326519212563994 1102 58-59 -0.11461870817212372 1102 60-61 -0.089522626149936 1102 62-63 -0.013903100927535661 1102 64-65 -0.016227084000526304 1102 66-67 -0.06871138468930127 1102 68-69 -0.1101411302410753 1102 70-71 -0.14600328775808435 1102 72-73 -0.112267913048953 1102 74-75 -0.19319715846179264 1102 76-77 -0.24961959040021497 1102 78-79 0.010925176485820032 1102 80-81 0.03556055489868726 1102 82-83 -0.09865430884019588 1102 84-85 -0.10778862387542887 1102 86-87 -0.11140327449290055 1102 88-89 -0.15960223742975188 1102 90-91 -0.2266622883437961 1102 92-93 -0.2467622916193477 1102 94-95 -0.26736616200312824 1102 96-97 -0.19843573722219077 1102 98-99 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1213 54-55 -0.21584133113818638 1213 56-57 -0.22590812346152234 1213 58-59 -0.26337840432119464 1213 60-61 -0.305732103470703 1213 62-63 -0.15508950134190513 1213 64-65 -0.1647202908273897 1213 66-67 -0.10020668332193594 1213 68-69 -0.03238554799884952 1213 70-71 -0.1727887415233269 1213 72-73 -0.13569805018848768 1213 74-75 -0.008388175742680914 1213 76-77 0.4564108943099825 1213 78-79 -0.04655440754277862 1213 80-81 -0.2444527297763699 1213 82-83 -0.29956334229395054 1213 84-85 -0.20173115351152404 1213 86-87 -0.20437705758097024 1213 88-89 -0.12581084230686201 1213 90-91 -0.13272369423658148 1213 92-93 0.01644416763465273 1213 94-95 -0.030413746634422978 1213 96-97 -0.007689423171363785 1213 98-99 0.037249082588708404 1213 100-101 0.3425191191866297 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 48.0 8 532.0 9 1687.0 10 1038.0 11 395.0 12 258.0 13 491.0 14 1101.0 15 2542.0 16 4635.0 17 7476.0 18 11073.0 19 15200.0 20 20781.0 21 28195.0 22 37316.0 23 48769.0 24 63612.0 25 83011.0 26 107761.0 27 140076.0 28 181264.0 29 235521.0 30 306304.0 31 408673.0 32 562904.0 33 822564.0 34 1334250.0 35 2540137.0 36 5349833.0 37 6581995.0 38 985714.0 39 102.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 21.0507290945017 22.434443477307696 30.45145939995238 26.06336802823822 2 27.919101887528754 22.663812291114123 29.035526817351144 20.381559004005982 3 21.977509974725 20.7227866268784 35.979519503390954 21.32018389500565 4 21.856272952073795 21.01785020289207 34.997663226717066 22.128213618317073 5 28.081327576305267 20.327337937748865 31.00521190067417 20.586122585271703 6 29.780581194311768 20.90288485038667 29.353159073605273 19.963374881696293 7 28.18068257593816 26.665515059898638 24.560196849324576 20.59360551483863 8 26.768686957072724 22.50356796944068 29.25832501058573 21.469420062900863 9 23.315319990787145 21.441042259442423 32.92418102654931 22.31945672322112 10-11 27.561406245085085 22.2060835181206 27.397686934189704 22.834823302604615 12-13 26.700177500521498 25.408171742617004 24.444403151479115 23.447247605382383 14-15 26.30557018671822 23.82642960931158 25.7453209810001 24.122679222970103 16-17 25.87036830295405 23.56643564127399 25.266172028929972 25.297024026841992 18-19 25.821309351750994 24.066587512559554 25.64831437959574 24.46378875609371 20-21 24.981945019623993 24.410185116037916 26.100931375987894 24.506938488350194 22-23 25.08289337428308 24.557713421208476 25.511873563754804 24.84751964075364 24-25 25.56358076283639 24.41634909475662 25.223907054773232 24.796163087633754 26-27 25.212758093180575 24.537084252355765 25.54107665677995 24.70908099768371 28-29 25.21190318859296 25.072387788744027 24.941260511554788 24.774448511108226 30-31 25.493458471249557 24.81372634805881 24.822783307837064 24.870031872854568 32-33 25.231535821005107 25.09811287355559 24.967815356701394 24.702535948737907 34-35 25.061349461862708 25.232048763757675 24.8963176744986 24.810284099881017 36-37 25.254953275915998 24.953638001249196 24.999112388243898 24.79229633459091 38-39 25.249304271535145 24.668171406572224 25.109557587859687 24.972966734032948 40-41 25.02851486314689 25.0850479571982 24.724223887648762 25.162213292006154 42-43 25.019845379786886 24.94445179505075 24.88190710365778 25.153795721504586 44-45 25.35615828005266 24.745130312603404 24.868583563906128 25.030127843437803 46-47 24.96645253821172 24.875410228480792 25.068334535228608 25.089802698078877 48-49 25.092982202845054 24.60761004999835 24.956790852640225 25.34261689451637 50-51 24.96201540887251 24.924688347323666 25.360868073818523 24.7524281699853 52-53 25.108925377905557 24.708658877251068 25.43746182016507 24.74495392467831 54-55 24.865783029332007 24.880820711424693 24.967780257318882 25.28561600192442 56-57 25.002785600776324 25.079429542154358 25.17751588282799 24.740268974241335 58-59 24.828061458373597 25.2522782490166 25.14460958724616 24.775050705363643 60-61 25.34907374742233 25.0935279620834 24.67486677567848 24.882531514815792 62-63 24.960502689070818 25.27683822570785 25.161523905110506 24.60113518011083 64-65 25.17880553841917 25.15232272064334 24.95712383523503 24.71174790570246 66-67 25.293984944500547 25.241973263285 25.06220944605862 24.40183234615583 68-69 25.177721387829834 24.92526180248876 25.41069290895007 24.486323900731335 70-71 25.003029383434832 25.366165562130078 25.05255835643788 24.578246697997205 72-73 24.94929333073192 25.002274726961087 25.313455866298263 24.734976076008724 74-75 24.999154492298715 24.929316184802712 25.294070093028477 24.777459229870097 76-77 24.86706546252518 24.978417509342982 25.171987849678285 24.982529178453554 78-79 25.080414774169746 24.87315040235802 24.988601264226165 25.057833559246067 80-81 24.98900384656662 24.766739726253363 25.303939414086322 24.940317013093697 82-83 24.752311530230372 25.515914235948458 25.138161251282714 24.593612982538456 84-85 24.934090507597205 25.209617948877533 25.001936942798892 24.85435460072637 86-87 24.810858479252506 25.30784905153051 25.175303814049187 24.705988655167793 88-89 24.858031963162006 26.282921625586425 24.488656174396553 24.37039023685502 90-91 24.924405949733412 25.38531963795342 24.704960609601688 24.985313802711477 92-93 24.693906198848044 25.70471975197949 24.68996555723313 24.911408491939337 94-95 24.75911072439422 25.87837781765097 24.36282203297125 24.999689424983558 96-97 24.67287150030731 25.407351884969177 24.63029423925031 25.289482375473206 98-99 24.944130203558487 25.34950245528354 24.60797594467912 25.098391396478853 100-101 25.224134414680265 25.938651774226447 24.043954688172747 24.793259122920546 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8702.0 1 5316.0 2 2937.0 3 3489.5 4 3172.0 5 2570.0 6 1755.0 7 1677.5 8 1693.5 9 1698.0 10 1606.0 11 1479.0 12 1379.5 13 1332.0 14 1346.0 15 1411.5 16 1488.5 17 1606.0 18 1743.0 19 1986.5 20 2326.5 21 2705.0 22 3305.0 23 4226.5 24 5332.5 25 6672.5 26 8525.0 27 10730.5 28 12878.0 29 15202.5 30 18881.0 31 24653.5 32 33114.0 33 43948.0 34 58215.5 35 78242.5 36 102775.5 37 131689.0 38 165718.0 39 215527.0 40 286988.5 41 362253.5 42 429848.0 43 520728.5 44 641827.0 45 797670.5 46 986799.0 47 1179249.0 48 1327147.5 49 1366653.5 50 1314027.5 51 1239123.5 52 1166141.0 53 1112719.0 54 1052947.0 55 993700.0 56 961624.0 57 835251.0 58 637514.5 59 478179.0 60 361336.0 61 277227.0 62 199573.0 63 130793.5 64 83172.5 65 51297.5 66 30285.5 67 18588.5 68 13486.0 69 10102.0 70 7766.5 71 5793.5 72 4326.5 73 3255.0 74 2405.0 75 1738.0 76 1266.0 77 943.0 78 743.0 79 594.0 80 403.5 81 244.5 82 171.0 83 138.0 84 103.5 85 75.5 86 60.0 87 47.5 88 35.0 89 32.0 90 34.5 91 26.0 92 22.0 93 18.0 94 14.5 95 15.0 96 13.5 97 8.5 98 5.0 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12473057933363707 2 0.0 3 0.0 4 0.3386126206044075 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.2687528350144784E-5 12-13 0.0012471549278209085 14-15 1.005770103081378E-5 16-17 0.0 18-19 0.0 20-21 8.297603350421367E-4 22-23 3.821926391709236E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.002212694226779031 38-39 0.007166111984454817 40-41 0.006695914461264274 42-43 0.0014030492937985221 44-45 0.0 46-47 0.0 48-49 1.5086551546220666E-5 50-51 0.0011214336649357365 52-53 0.01197872192769921 54-55 0.009436637992161028 56-57 0.008838204780827608 58-59 0.012579669564290335 60-61 0.0028413005412048927 62-63 2.2881269845101347E-4 64-65 0.0033643009948072086 66-67 0.01119673567255344 68-69 0.019411362989470594 70-71 0.02502858901518009 72-73 0.018873275984322056 74-75 0.003809354265420719 76-77 0.014198959430251352 78-79 0.007422583360740568 80-81 0.005199831432930724 82-83 0.003545339613361857 84-85 0.010673735218951123 86-87 0.01504380631683971 88-89 0.012710419677690913 90-91 0.03042454561821168 92-93 0.013633213747268077 94-95 0.014005348685408188 96-97 0.007075592675177492 98-99 0.012189933649346299 100-101 0.13733790757576214 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.988526E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.725966323084887 #Duplication Level Percentage of deduplicated Percentage of total 1 77.47127708050779 18.380809110185055 2 10.401910310014845 4.9359074742232325 3 3.574138632256496 2.543996784688629 4 1.8731576465247497 1.7776990095710057 5 1.018827302225529 1.2086331130821162 6 0.6777326747665385 0.964791757053908 7 0.5880403998896103 0.9766278707075983 8 0.4166190967188297 0.7907752526643996 9 0.35951415603256087 0.7676838682830741 >10 2.5642030286964994 12.90174280842965 >50 0.47475786311119783 7.997604623715045 >100 0.4860403152051619 23.682468521369355 >500 0.06298529621124245 10.333974244866969 >1k 0.03045621458685954 12.206200939641702 >5k 3.399832520638619E-4 0.5310846215181989 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.6092321649302047E-4 0.0 0.0 2 1.7098091752383425E-4 0.0 0.0 3 1.7600976803924112E-4 0.0 0.0 4 1.7600976803924112E-4 0.0 0.0 5 1.7600976803924112E-4 0.0 0.0 6 1.7600976803924112E-4 0.0 0.0 7 1.860674690700549E-4 0.0 0.0 8 1.910963195854618E-4 0.0 0.0 9 2.1624057216249623E-4 0.0 0.0 10-11 2.589858015434548E-4 0.0 2.514425257703445E-6 12-13 3.218464329860409E-4 0.0 5.02885051540689E-6 14-15 4.551109716443235E-4 0.0 5.02885051540689E-6 16-17 5.682601082409785E-4 0.0 5.02885051540689E-6 18-19 7.24154474218592E-4 0.0 5.02885051540689E-6 20-21 9.55481597927309E-4 0.0 5.02885051540689E-6 22-23 0.0011918375721514328 0.0 5.02885051540689E-6 24-25 0.0013980204432831153 0.0 5.02885051540689E-6 26-27 0.0016896937731767149 0.0 5.02885051540689E-6 28-29 0.0020165690566781623 0.0 5.02885051540689E-6 30-31 0.002398761695849086 0.0 5.02885051540689E-6 32-33 0.0028765024948127407 0.0 5.02885051540689E-6 34-35 0.0035729982911965946 0.0 1.005770103081378E-5 36-37 0.004352470121084662 0.0 1.005770103081378E-5 38-39 0.00542361528086633 0.0 1.005770103081378E-5 40-41 0.006718544288583604 0.0 1.005770103081378E-5 42-43 0.00846104099217209 0.0 1.005770103081378E-5 44-45 0.010598302461220021 0.0 1.005770103081378E-5 46-47 0.013608069494691042 0.0 1.005770103081378E-5 48-49 0.01722129859001089 0.0 1.005770103081378E-5 50-51 0.021802581409546568 0.0 1.005770103081378E-5 52-53 0.027480153641440946 0.0 1.005770103081378E-5 54-55 0.03448785683466045 0.0 1.005770103081378E-5 56-57 0.04310227776755245 0.0 1.005770103081378E-5 58-59 0.05386150344526549 0.0 1.005770103081378E-5 60-61 0.06714018323119739 0.0 1.005770103081378E-5 62-63 0.0837932217129673 0.0 1.005770103081378E-5 64-65 0.10444671077974339 0.0 1.005770103081378E-5 66-67 0.12989017996244456 0.0 1.005770103081378E-5 68-69 0.1610992262610597 0.0 1.005770103081378E-5 70-71 0.19922545644361703 0.0 1.005770103081378E-5 72-73 0.24597616526009716 0.0 1.005770103081378E-5 74-75 0.30295807045017265 0.0 1.005770103081378E-5 76-77 0.3718382359597008 0.0 1.005770103081378E-5 78-79 0.4572960071932678 0.0 1.005770103081378E-5 80-81 0.5600932550039577 0.0 1.005770103081378E-5 82-83 0.6827242892474124 0.0 1.005770103081378E-5 84-85 0.8300268641194533 0.0 1.005770103081378E-5 86-87 1.000188079009276 0.0 1.005770103081378E-5 88-89 1.2004067334296862 0.0 1.005770103081378E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 149960 0.0 69.54428 1 TTTGTGT 159095 0.0 65.53733 2 TTGTGTT 182630 0.0 58.834427 3 TAACTTT 17100 0.0 57.162685 1 AACTTTG 17255 0.0 56.85386 2 TGTGTTT 196220 0.0 55.046135 4 ACTTTGT 39075 0.0 54.999992 1 GTGTTTG 225060 0.0 48.003075 5 GTTTGAG 183280 0.0 34.226093 2 TGTTTGA 343100 0.0 34.01802 1 TTTGAGT 69250 0.0 33.42384 3 TTGAGTC 24910 0.0 33.223442 4 TTTGAGG 68305 0.0 32.867348 8 GTTTGAA 29420 0.0 31.40174 2 TTGAGGG 35980 0.0 30.485298 9 TTTGAGC 48720 0.0 28.760838 8 TTGAGGC 13585 0.0 27.053343 9 GAGGGCG 8360 0.0 26.183897 6 GTTTGAC 72305 0.0 24.92201 7 TTGAGTG 36445 0.0 24.538324 4 >>END_MODULE