FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l04n01_7331.34100000035c1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l04n01_7331.34100000035c1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19885260
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1499600.069.544281
TTTGTGT1590950.065.537332
TTGTGTT1826300.058.8344273
TAACTTT171000.057.1626851
AACTTTG172550.056.853862
TGTGTTT1962200.055.0461354
ACTTTGT390750.054.9999921
GTGTTTG2250600.048.0030755
GTTTGAG1832800.034.2260932
TGTTTGA3431000.034.018021
TTTGAGT692500.033.423843
TTGAGTC249100.033.2234424
TTTGAGG683050.032.8673488
GTTTGAA294200.031.401742
TTGAGGG359800.030.4852989
TTTGAGC487200.028.7608388
TTGAGGC135850.027.0533439
GAGGGCG83600.026.1838976
GTTTGAC723050.024.922017
TTGAGTG364450.024.5383244
Produced by FastQC (version 0.11.2)