##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l04n01_6957.34100000035bda.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26521818 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.72606331134615 33.0 33.0 33.0 33.0 33.0 2 32.75920798491265 33.0 33.0 33.0 33.0 33.0 3 32.79167943916967 33.0 33.0 33.0 33.0 33.0 4 36.60385483378251 37.0 37.0 37.0 37.0 37.0 5 36.62825738416575 37.0 37.0 37.0 37.0 37.0 6 36.63176517537372 37.0 37.0 37.0 37.0 37.0 7 36.60325589294067 37.0 37.0 37.0 37.0 37.0 8 36.560211822583206 37.0 37.0 37.0 37.0 37.0 9 36.58052359004952 37.0 37.0 37.0 37.0 37.0 10-11 36.58090623727227 37.0 37.0 37.0 37.0 37.0 12-13 36.54989054671893 37.0 37.0 37.0 37.0 37.0 14-15 38.603713120269504 40.0 37.0 40.0 37.0 40.0 16-17 38.584983823507116 40.0 37.0 40.0 37.0 40.0 18-19 38.539719939259065 40.0 37.0 40.0 37.0 40.0 20-21 38.476495314159834 40.0 37.0 40.0 37.0 40.0 22-23 38.42778174180971 40.0 37.0 40.0 37.0 40.0 24-25 38.378143383685085 40.0 37.0 40.0 37.0 40.0 26-27 38.272719125061485 40.0 37.0 40.0 37.0 40.0 28-29 38.2055994426928 40.0 37.0 40.0 37.0 40.0 30-31 38.147551517773024 40.0 37.0 40.0 37.0 40.0 32-33 38.04219454337557 40.0 37.0 40.0 37.0 40.0 34-35 37.955016356721856 40.0 37.0 40.0 35.0 40.0 36-37 37.85401526773164 40.0 37.0 40.0 33.0 40.0 38-39 37.72599776154108 40.0 37.0 40.0 33.0 40.0 40-41 37.55071788442255 40.0 37.0 40.0 33.0 40.0 42-43 37.429532074309535 40.0 37.0 40.0 33.0 40.0 44-45 37.31162339625436 40.0 37.0 40.0 33.0 40.0 46-47 37.148432792201504 37.0 37.0 40.0 33.0 40.0 48-49 36.95148021149983 37.0 37.0 40.0 33.0 40.0 50-51 36.751442906364865 37.0 37.0 40.0 33.0 40.0 52-53 36.519244759163946 37.0 37.0 40.0 33.0 40.0 54-55 36.263518775372034 37.0 37.0 40.0 33.0 40.0 56-57 35.97698713564809 37.0 37.0 40.0 33.0 40.0 58-59 35.71847580358179 37.0 35.0 40.0 33.0 40.0 60-61 35.43207109708693 37.0 33.0 37.0 33.0 40.0 62-63 35.12554908566223 37.0 33.0 37.0 27.0 40.0 64-65 34.89713365426156 37.0 33.0 37.0 27.0 40.0 66-67 34.62678482297103 37.0 33.0 37.0 27.0 40.0 68-69 34.37018504915463 37.0 33.0 37.0 27.0 37.0 70-71 34.104360662606155 37.0 33.0 37.0 27.0 37.0 72-73 33.87714371616606 37.0 33.0 37.0 27.0 37.0 74-75 33.64581430654565 37.0 33.0 37.0 27.0 37.0 76-77 30.832319526512094 33.0 30.0 35.0 24.5 37.0 78-79 33.3795196279531 35.0 33.0 37.0 27.0 37.0 80-81 33.83046923857181 37.0 33.0 37.0 27.0 37.0 82-83 33.81554128378379 37.0 33.0 37.0 27.0 37.0 84-85 33.75123027011195 37.0 33.0 37.0 27.0 37.0 86-87 33.63396798439685 37.0 33.0 37.0 27.0 37.0 88-89 33.44214058025736 37.0 33.0 37.0 27.0 37.0 90-91 33.30306071024241 37.0 33.0 37.0 27.0 37.0 92-93 33.188788000882894 37.0 33.0 37.0 27.0 37.0 94-95 33.07463474034849 35.0 33.0 37.0 27.0 37.0 96-97 32.95143194934827 33.0 33.0 37.0 27.0 37.0 98-99 32.797480474377736 33.0 33.0 37.0 27.0 37.0 100-101 30.891324493667817 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10923015810002568 2306 2 0.08514801652732018 2306 3 0.0848721362112812 2306 4 -0.1430912509458011 2306 5 -0.064531062305619 2306 6 0.07339800324529477 2306 7 0.09997764570399426 2306 8 0.17001510778496254 2306 9 0.13573837481339268 2306 10-11 0.10182768164890632 2306 12-13 0.11243759575514645 2306 14-15 0.20917542135374134 2306 16-17 0.21639537946239784 2306 18-19 0.1737984071389036 2306 20-21 0.15215813479749585 2306 22-23 0.1736156579353434 2306 24-25 0.16793843197008584 2306 26-27 0.1939454673785974 2306 28-29 0.1431158044140588 2306 30-31 0.1366222026506918 2306 32-33 0.17545017943483288 2306 34-35 0.17677768810893468 2306 36-37 0.12704933458940815 2306 38-39 0.12946390020495357 2306 40-41 0.17357279168346906 2306 42-43 0.13943172284626115 2306 44-45 0.06290050683195858 2306 46-47 0.0310784689366983 2306 48-49 0.03483459005720846 2306 50-51 -0.03744347191145181 2306 52-53 0.0018664386017306356 2306 54-55 0.05703312332249055 2306 56-57 0.0387762940145393 2306 58-59 -0.03547962029791307 2306 60-61 -0.07044600426328174 2306 62-63 -0.004288614881176045 2306 64-65 -0.06991594659374556 2306 66-67 -0.12481843958116201 2306 68-69 -0.275100285745566 2306 70-71 -0.20809481994299261 2306 72-73 -0.14653608375395066 2306 74-75 -0.21934266632022315 2306 76-77 -0.8219749299305654 2306 78-79 -0.17713666450742238 2306 80-81 0.0031313358442588424 2306 82-83 -0.12081020012152521 2306 84-85 -0.19664395135583135 2306 86-87 -0.17762330933737758 2306 88-89 -0.21299910723141835 2306 90-91 -0.255980827139517 2306 92-93 -0.34595303691833124 2306 94-95 -0.32289395343133265 2306 96-97 -0.287298234703421 2306 98-99 -0.15694065457926598 2306 100-101 -0.47173612396705167 2307 1 0.11826055080521769 2307 2 0.09676270014231392 2307 3 0.08277079111876162 2307 4 -0.11191850035880435 2307 5 0.00955863162141668 2307 6 0.055387558942207704 2307 7 0.09763465288149575 2307 8 0.14327572228405927 2307 9 0.1080121339952953 2307 10-11 0.08561216898052493 2307 12-13 0.08131424521589992 2307 14-15 0.1582502768544103 2307 16-17 0.12428405266638265 2307 18-19 0.11369717680357638 2307 20-21 0.14170176532064005 2307 22-23 0.14709306205742223 2307 24-25 0.14324742441466043 2307 26-27 0.10481921499848568 2307 28-29 0.08449783273694322 2307 30-31 0.13793102680554625 2307 32-33 0.16566617655919202 2307 34-35 0.17746664785924082 2307 36-37 0.09172617655746507 2307 38-39 0.04378457046968265 2307 40-41 0.10489262802064303 2307 42-43 0.004356340218315324 2307 44-45 0.038173590228950616 2307 46-47 -0.021731617870869968 2307 48-49 0.00493004556005161 2307 50-51 -0.04770704320483077 2307 52-53 -0.07145561853186422 2307 54-55 0.019875007007719603 2307 56-57 -0.15015637757760203 2307 58-59 -0.1850000961434617 2307 60-61 -0.17576940191204216 2307 62-63 -0.19429763171964964 2307 64-65 -0.25890099763566354 2307 66-67 -0.26016595208585613 2307 68-69 -0.38449419376634353 2307 70-71 -0.36246093795407575 2307 72-73 -0.3642379877302133 2307 74-75 -0.3778321465426657 2307 76-77 -0.9341245393272395 2307 78-79 -0.26370182886139304 2307 80-81 -0.12095552486115224 2307 82-83 -0.1787178987230078 2307 84-85 -0.23545787127383022 2307 86-87 -0.25907882898165724 2307 88-89 -0.22752466662121407 2307 90-91 -0.3663621781747679 2307 92-93 -0.38838722786044855 2307 94-95 -0.3347568498756388 2307 96-97 -0.21830255571417467 2307 98-99 -0.267856497690488 2307 100-101 -0.7366878736114053 2201 1 0.10442113157706245 2201 2 0.06985458955171708 2201 3 0.06695142256863562 2201 4 -0.15548033420561325 2201 5 -0.03773031468114141 2201 6 0.03520653310560107 2201 7 0.09690693127765826 2201 8 0.11823530034341445 2201 9 0.1024871687460589 2201 10-11 0.08179355140985223 2201 12-13 0.07365466592304415 2201 14-15 0.15462177146509504 2201 16-17 0.12489364722012652 2201 18-19 0.1173133887563722 2201 20-21 0.13383124560855464 2201 22-23 0.06904155596400585 2201 24-25 0.11618559395269301 2201 26-27 0.13384090373540403 2201 28-29 0.12454429403593537 2201 30-31 0.10128814461599944 2201 32-33 0.13830304268977045 2201 34-35 0.10185682176526711 2201 36-37 0.036780412832086995 2201 38-39 0.06259603064378894 2201 40-41 0.13452384901347614 2201 42-43 0.06322299376922302 2201 44-45 0.04925605057254501 2201 46-47 0.007576671987926886 2201 48-49 -0.001730910057780477 2201 50-51 0.04330679110597657 2201 52-53 -0.005667469496039246 2201 54-55 0.0028222246811608898 2201 56-57 -0.02602196944905444 2201 58-59 -0.04040651279402141 2201 60-61 -0.08096486983699691 2201 62-63 0.006184655161895591 2201 64-65 -0.08140852726170067 2201 66-67 -0.1379554044751501 2201 68-69 -0.18603493514944347 2201 70-71 -0.2291001188224655 2201 72-73 -0.19710559294261287 2201 74-75 -0.34439608618040296 2201 76-77 -0.883670624949616 2201 78-79 -0.16175171604211158 2201 80-81 -0.030130691753903704 2201 82-83 -0.020844579762197668 2201 84-85 -0.13403727954338507 2201 86-87 -0.10515605360878055 2201 88-89 -0.09097311926587537 2201 90-91 -0.17422615869233482 2201 92-93 -0.2567455482264904 2201 94-95 -0.1482080726967112 2201 96-97 -0.12493964711367056 2201 98-99 -0.23928488993865926 2201 100-101 -0.5678424445125287 1101 1 0.060005078410704016 1101 2 0.08206487625542991 1101 3 0.06318850503514994 1101 4 -0.16798959044987782 1101 5 -0.04674167292746745 1101 6 0.050986023742417785 1101 7 0.06237282938767663 1101 8 0.1562609832040991 1101 9 0.08698977868080959 1101 10-11 0.06934968571563616 1101 12-13 0.07472827779808 1101 14-15 0.15340081476899314 1101 16-17 0.12846767407179982 1101 18-19 0.12021486106070967 1101 20-21 0.13033156753274255 1101 22-23 0.12730072206370835 1101 24-25 0.11230215486538242 1101 26-27 0.14252343597264883 1101 28-29 0.11264239457718617 1101 30-31 0.11207685920506094 1101 32-33 0.10452779377929744 1101 34-35 0.11571808249610172 1101 36-37 0.10397407251603141 1101 38-39 0.10960210675002457 1101 40-41 0.1536678121364332 1101 42-43 0.12943817935005342 1101 44-45 0.13157991267062386 1101 46-47 0.11312590408996925 1101 48-49 0.08617115057193558 1101 50-51 0.041926684523652114 1101 52-53 0.030885745160723843 1101 54-55 0.06358718776189676 1101 56-57 0.04864518972724596 1101 58-59 -0.02131210266909278 1101 60-61 -0.019000352765374373 1101 62-63 -0.0023615468877409285 1101 64-65 -0.04049080265362903 1101 66-67 -0.08999395672046262 1101 68-69 -0.07563120256392608 1101 70-71 -0.1703324223903948 1101 72-73 -0.011647225782986936 1101 74-75 -0.15065643689944608 1101 76-77 -0.6246142956385512 1101 78-79 0.05875502521559639 1101 80-81 0.05395785086165006 1101 82-83 0.08055142220860745 1101 84-85 0.02259566332312346 1101 86-87 0.08412930424823628 1101 88-89 0.10759558245393208 1101 90-91 0.07438468653169394 1101 92-93 -0.05388781314231039 1101 94-95 -0.023259712627186957 1101 96-97 0.04511814179698348 1101 98-99 -0.030338032203445664 1101 100-101 -0.3016030672236134 2304 1 0.0900867141168149 2304 2 0.049181668424765235 2304 3 0.04964278160918667 2304 4 -0.1653120458664432 2304 5 -0.06750519515020414 2304 6 0.06581949176596424 2304 7 0.05954317887733396 2304 8 0.14885674748614264 2304 9 0.08396278825087222 2304 10-11 0.056077086158246914 2304 12-13 0.06100948114057303 2304 14-15 0.0676519772255304 2304 16-17 0.09047777336969176 2304 18-19 0.07271558648494647 2304 20-21 0.054759894713406254 2304 22-23 0.0914553483793128 2304 24-25 0.05433256891002003 2304 26-27 0.10242171823708901 2304 28-29 0.09872420181958574 2304 30-31 0.10346370705376984 2304 32-33 0.12482425643379713 2304 34-35 0.1285883570602806 2304 36-37 0.07465564669892899 2304 38-39 0.013260957784652305 2304 40-41 0.11571493257335419 2304 42-43 0.12147734872790039 2304 44-45 0.03813292823831915 2304 46-47 0.05668130086104384 2304 48-49 0.014207385160112551 2304 50-51 -0.0831210643093172 2304 52-53 -0.01951675043908807 2304 54-55 0.008831105312481213 2304 56-57 -0.0900829658152631 2304 58-59 -0.11319169503422444 2304 60-61 -0.04671078016498598 2304 62-63 0.049191942966331226 2304 64-65 -0.005321902459670014 2304 66-67 7.370051882560347E-5 2304 68-69 -0.15534469344498802 2304 70-71 -0.12544419736065748 2304 72-73 -0.08419012694050565 2304 74-75 -0.12482173863907775 2304 76-77 -0.2865329537516246 2304 78-79 0.0382371814508744 2304 80-81 0.10763634934865252 2304 82-83 0.049782719578089996 2304 84-85 -0.03832135302032924 2304 86-87 0.02874730127109615 2304 88-89 0.022538364311451176 2304 90-91 -0.0031624406750907497 2304 92-93 -0.09043739650070393 2304 94-95 -0.026889869114192777 2304 96-97 0.06475499975103816 2304 98-99 -7.62724266685666E-4 2304 100-101 -0.24372592631221934 2202 1 0.08561870241928915 2202 2 0.05505548171348096 2202 3 0.013249493814186053 2202 4 -0.22305189725258145 2202 5 -0.0881854198460843 2202 6 0.008229036966753256 2202 7 0.030075302717925467 2202 8 0.1513012020010791 2202 9 0.03449424068600848 2202 10-11 0.015531456462930748 2202 12-13 0.03595237595158807 2202 14-15 0.01812988043761976 2202 16-17 0.00823056545821288 2202 18-19 0.01943911937936349 2202 20-21 0.037839240461345014 2202 22-23 0.012678618113866946 2202 24-25 0.03100342703327641 2202 26-27 0.07282117024553258 2202 28-29 0.06260733952000663 2202 30-31 0.042233753126097895 2202 32-33 0.001375227679673685 2202 34-35 -0.009368585430443943 2202 36-37 0.006772069150535742 2202 38-39 -0.011936157415902926 2202 40-41 0.024192796493565538 2202 42-43 0.06236465854881601 2202 44-45 -0.06489113028803928 2202 46-47 0.018607202199582673 2202 48-49 0.01787370098416119 2202 50-51 -0.05375201250367212 2202 52-53 -0.04660167336440679 2202 54-55 -0.023868873392800083 2202 56-57 -0.006769505154970545 2202 58-59 -0.018241817672397076 2202 60-61 -0.08076432956735857 2202 62-63 -0.054438601771792605 2202 64-65 -0.057515779052039306 2202 66-67 -0.043460812380857305 2202 68-69 -0.14145074956405068 2202 70-71 -0.18811890716722246 2202 72-73 -0.10537867037168525 2202 74-75 -0.14317878136085938 2202 76-77 -0.19547909891229054 2202 78-79 0.019984204378197035 2202 80-81 0.056645285538557744 2202 82-83 -0.00349615821229321 2202 84-85 -0.004893765601273969 2202 86-87 -0.009106965808328482 2202 88-89 -0.007921609736634139 2202 90-91 -0.023534062036532077 2202 92-93 -0.0023638442916151803 2202 94-95 0.002260748117272726 2202 96-97 -0.010517263585008152 2202 98-99 -0.03263338806112159 2202 100-101 -0.12815907934285775 1102 1 0.07493449787897077 1102 2 0.06417783374315178 1102 3 0.04215287104822352 1102 4 -0.1666609054398691 1102 5 -0.05718572190667004 1102 6 -0.008266821798578405 1102 7 0.031152912975954905 1102 8 0.12247869578600756 1102 9 0.05128465260015247 1102 10-11 0.059990023165262585 1102 12-13 0.040664454626984536 1102 14-15 0.04638932816326502 1102 16-17 0.020510652778362726 1102 18-19 0.002727630202457476 1102 20-21 -0.03058054160316459 1102 22-23 0.015037839363301941 1102 24-25 0.03902675461079497 1102 26-27 0.11111249721898986 1102 28-29 0.10072159092898403 1102 30-31 0.05068632478046453 1102 32-33 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48-49 -0.2697448023362128 1213 50-51 -0.24027500724248085 1213 52-53 -0.3013770215395155 1213 54-55 -0.2060664240877088 1213 56-57 -0.2245964456531624 1213 58-59 -0.2531197044966902 1213 60-61 -0.21115661324893864 1213 62-63 -0.10580319361025659 1213 64-65 -0.12672450556362946 1213 66-67 -0.011817350396768234 1213 68-69 0.05619846745339174 1213 70-71 -0.08847195894487214 1213 72-73 -0.03878883626487806 1213 74-75 0.06285500617795492 1213 76-77 0.5232436496351127 1213 78-79 0.007371314840206367 1213 80-81 -0.18630869094456415 1213 82-83 -0.2634885110631018 1213 84-85 -0.19698520696979216 1213 86-87 -0.17347056589355958 1213 88-89 -0.04027183598540063 1213 90-91 -0.053148655575462556 1213 92-93 0.09858532690165589 1213 94-95 0.029164466820354562 1213 96-97 0.01802922807667784 1213 98-99 0.13277457480826627 1213 100-101 0.37554850405162554 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 69.0 8 749.0 9 2233.0 10 1581.0 11 608.0 12 528.0 13 964.0 14 2631.0 15 6443.0 16 11830.0 17 18339.0 18 25083.0 19 33061.0 20 41882.0 21 52266.0 22 64920.0 23 81424.0 24 101246.0 25 127933.0 26 160853.0 27 203072.0 28 256823.0 29 325994.0 30 423468.0 31 558140.0 32 757077.0 33 1074290.0 34 1679937.0 35 3142387.0 36 6750840.0 37 8951492.0 38 1663615.0 39 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.770164362250817 21.678944166244722 31.832016261810193 27.718875209694264 2 26.032453732998245 20.866555980438445 34.00706165768877 19.093928628874536 3 20.02092013450963 19.423001092911505 42.8417199756065 17.714358796972366 4 19.441534423111154 18.49462813067422 42.54049118503455 19.523346261180084 5 30.304412012781324 18.028368191049346 34.52665650597557 17.140563290193754 6 29.909405154654177 18.306648511048525 35.043193494503285 16.740752839794016 7 30.78690533205529 25.214210428561117 26.925639109656814 17.07324512972678 8 27.719472322749517 20.56776801650626 33.3439283837933 18.36883127695092 9 21.46907500835727 19.26893548549349 39.784802836668284 19.477186669480954 10-11 28.633136275389955 20.401626057950043 30.555686056117587 20.40955161054242 12-13 27.09797658878923 26.880886881144406 24.276430640327632 21.74470588973873 14-15 27.124912779357736 23.34743606188686 25.765450920445954 23.762200238309454 16-17 26.428314604979192 22.760847314463888 25.36416998261582 25.4466680979411 18-19 26.177969775676768 23.953077047734812 26.16712210301722 23.7018310735712 20-21 24.265211574876005 25.166786820393057 26.96951312855418 23.59848847617676 22-23 24.728869415040243 25.119686798617373 26.41017804198385 23.74126574435853 24-25 24.81301432654428 25.28442054764119 26.085843738163046 23.81672138765148 26-27 24.96965328696547 25.12980822053752 26.12520001456914 23.77533847792787 28-29 24.706187185207288 25.599700593677248 25.738627721523464 23.955484499591996 30-31 25.021480427925415 25.374561427123886 25.575658124190433 24.028300020760266 32-33 24.878498148203867 25.560412185921795 25.42548553798235 24.135604127891988 34-35 25.083178687071907 25.4995717111097 25.574082063303504 23.843167538514894 36-37 25.060286960104587 25.523778874569885 25.529182082352165 23.886752082973363 38-39 25.14180947727845 25.262398108168753 25.545479730646175 24.05031268390662 40-41 24.954551326608804 25.551771706407827 25.268212406729916 24.22546456025345 42-43 24.917950286298762 25.70245953261705 25.26738480566332 24.112205375420874 44-45 24.864155240036713 25.401955853855874 25.51510986162412 24.218779044483302 46-47 24.55868786973804 25.68706639944517 25.369776687254248 24.384469043562547 48-49 24.716615338476295 25.210919781389002 25.26757314818561 24.804891731949095 50-51 24.57530997362309 25.31594896599043 25.89067722268475 24.21806383770173 52-53 24.844387986793496 25.20660358427313 25.77977301477678 24.1692354141566 54-55 24.606410470695028 25.155645326492287 25.318524522520775 24.919419680291906 56-57 24.65610691782468 25.37011528909644 25.54447750480429 24.42930028827459 58-59 24.80728695582559 25.388948628087437 25.461490415269772 24.342274000817202 60-61 24.880158019268418 25.476095524729875 25.176718457752994 24.467027998248714 62-63 24.56267644595973 25.86256033184231 25.283047367135815 24.291715855062147 64-65 24.852063598574116 25.478493117542346 25.128073795120503 24.54136948876304 66-67 24.996017466996637 25.595934284896487 25.28203960292284 24.126008645184037 68-69 24.681969688024477 25.09438845994505 25.70793018437817 24.515711667652308 70-71 24.742113840283587 25.255752473035265 25.371757897030868 24.630375789650287 72-73 24.234005982308908 24.978598515389383 26.057210712644167 24.730184789657535 74-75 24.482017596904626 24.83329394609507 25.858626554590096 24.826061902410206 76-77 24.227327264737518 24.87098882191606 25.71196880193043 25.189715111415996 78-79 24.744975952354487 25.05899542727463 25.136000004826574 25.06002861554431 80-81 24.438381486813572 24.885516075693577 25.57401179049666 25.102090646996196 82-83 24.49611319561069 25.524219834728758 25.444441205906877 24.53522576375368 84-85 24.470009658350513 25.642530318144097 25.334159205952588 24.553300817552806 86-87 24.348914706058817 25.52563955154868 25.55150892951722 24.57393681287529 88-89 24.283872578304585 26.583530939223238 24.852376073932586 24.28022040853959 90-91 24.34704034165536 26.11553498156578 24.83291868033988 24.70450599643898 92-93 24.161967145793266 26.41064958457332 24.565763139999888 24.861620129633526 94-95 24.166746863426777 26.555280406086222 24.428992154494257 24.84898057599274 96-97 23.97353827179459 26.27641496154538 24.400360166442177 25.34968660021785 98-99 24.203496935294957 26.17009055957492 24.0562720824788 25.57014042265132 100-101 24.510194756178862 26.884379700970936 23.621383771397632 24.984041771452567 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11518.0 1 6921.0 2 4391.0 3 6057.0 4 6876.0 5 5616.5 6 2888.0 7 2647.5 8 2676.0 9 2523.5 10 2317.5 11 2287.0 12 2244.0 13 2107.5 14 2095.0 15 2164.5 16 2249.5 17 2411.0 18 2610.0 19 2931.0 20 3398.5 21 3805.0 22 4274.5 23 5126.0 24 5987.0 25 6942.0 26 8150.0 27 9436.5 28 11486.0 29 14546.0 30 18544.0 31 24440.5 32 35839.5 33 55731.0 34 82823.0 35 128357.5 36 184888.5 37 255493.0 38 349869.5 39 451391.0 40 560314.5 41 684748.5 42 814326.0 43 926702.5 44 1080896.5 45 1291496.5 46 1512811.5 47 1705988.5 48 1797974.5 49 1804615.5 50 1699410.0 51 1578653.0 52 1496014.0 53 1377795.5 54 1229614.0 55 1073899.5 56 936755.5 57 740671.0 58 550749.5 59 437695.5 60 343254.0 61 269610.0 62 209916.0 63 150380.0 64 101280.5 65 67254.5 66 47347.0 67 35670.5 68 29398.0 69 25037.5 70 22683.0 71 21260.0 72 20852.5 73 20702.5 74 19025.5 75 16211.0 76 13617.5 77 11810.0 78 11150.5 79 10934.5 80 9086.5 81 6418.0 82 4720.0 83 3845.5 84 3009.0 85 2160.0 86 1777.0 87 1762.0 88 1792.5 89 1804.0 90 1575.0 91 1139.0 92 850.0 93 649.0 94 550.5 95 549.0 96 494.5 97 389.5 98 248.0 99 101.0 100 49.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12538356156429398 2 0.0 3 0.0 4 0.33637588494122084 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.0163844725878146E-5 12-13 0.0011066360533806543 14-15 0.0 16-17 0.0 18-19 0.0 20-21 8.973743805948747E-4 22-23 3.619661367105377E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0020190923563384684 38-39 0.007182765525349732 40-41 0.006756701218596704 42-43 0.0014968807945217027 44-45 0.0 46-47 0.0 48-49 1.8852402953673838E-5 50-51 0.0012348323934656365 52-53 0.012139062261870585 54-55 0.009314972299410244 56-57 0.008713580645188047 58-59 0.012478405515036714 60-61 0.0028222047221649737 62-63 2.4319599810239254E-4 64-65 0.003308596718369759 66-67 0.010902344628109581 68-69 0.01961592527329763 70-71 0.025181154625222146 72-73 0.018724206613588857 74-75 0.0036215466074007445 76-77 0.01420340038529787 78-79 0.0074127648413845545 80-81 0.00524662374200743 82-83 0.003510317429974069 84-85 0.010546034212285147 86-87 0.015002742270533642 88-89 0.012576438010395818 90-91 0.030473024134318395 92-93 0.013575615366940531 94-95 0.013973401069263052 96-97 0.007001782456994464 98-99 0.01205611168887442 100-101 0.13673836386329172 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.6521818E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.39888129441497 #Duplication Level Percentage of deduplicated Percentage of total 1 72.18797499337235 12.559900077938815 2 11.426540947545877 3.976180591042453 3 4.360087837694398 2.2758195216380184 4 2.30964387562516 1.6074087849749872 5 1.5107831826115212 1.3142968627928155 6 0.8432062383088985 0.8802507148228021 7 0.771055102764812 0.939084734312053 8 0.548259928745737 0.7631287534985188 9 0.3899362700594397 0.6106009387636016 >10 4.020052758231955 14.955755217665502 >50 0.6941868049964246 8.506332927362486 >100 0.8108108404750911 28.717787218260177 >500 0.09068770868375942 10.728787471418839 >1k 0.035551693249831706 10.469150060750264 >5k 9.580254434675323E-4 1.0409559215287993 >10k+ 2.637921910421244E-4 0.6545602032300857 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.772125877645341E-4 0.0 0.0 2 1.772125877645341E-4 0.0 0.0 3 1.8098306835526886E-4 0.0 0.0 4 1.9229451012747317E-4 0.0 0.0 5 1.9229451012747317E-4 0.0 0.0 6 1.998354713089427E-4 0.0 0.0 7 2.2999931603482084E-4 0.0 0.0 8 2.6016316076069897E-4 0.0 0.0 9 2.7901556371437284E-4 0.0 0.0 10-11 3.751628187781094E-4 0.0 0.0 12-13 5.184410812260306E-4 0.0 0.0 14-15 7.672928002145252E-4 0.0 0.0 16-17 0.0010632755265872046 0.0 0.0 18-19 0.0013573730126645164 0.0 0.0 20-21 0.0018230273656202603 0.0 0.0 22-23 0.0023282717647787193 0.0 0.0 24-25 0.002793926117734463 0.0 0.0 26-27 0.003323678640732698 0.0 0.0 28-29 0.00397785702322518 0.0 0.0 30-31 0.004780969389051686 0.0 0.0 32-33 0.0056330980025577435 0.0 0.0 34-35 0.006717111172393989 0.0 0.0 36-37 0.008204565765438855 0.0 0.0 38-39 0.009967265441607358 0.0 0.0 40-41 0.012097586975372502 0.0 0.0 42-43 0.014716185745637798 0.0 0.0 44-45 0.01820953601295356 0.0 0.0 46-47 0.02246263811930238 0.0 0.0 48-49 0.02792606449527706 0.0 0.0 50-51 0.03476760152716529 0.0 0.0 52-53 0.043219133771297276 0.0 0.0 54-55 0.053388119924508946 0.0 0.0 56-57 0.06665832636359996 3.770480590734768E-6 0.0 58-59 0.08262254118477097 3.770480590734768E-6 0.0 60-61 0.10312075891630054 3.770480590734768E-6 0.0 62-63 0.12873174832886644 3.770480590734768E-6 0.0 64-65 0.16206091151066643 3.770480590734768E-6 0.0 66-67 0.20212415302751868 3.770480590734768E-6 0.0 68-69 0.2512912199307001 3.770480590734768E-6 0.0 70-71 0.3114021067484891 3.770480590734768E-6 0.0 72-73 0.3799324767253889 3.770480590734768E-6 0.0 74-75 0.4598195342415818 3.770480590734768E-6 0.0 76-77 0.552773192244966 3.770480590734768E-6 0.0 78-79 0.6652221201427444 3.770480590734768E-6 0.0 80-81 0.7982823801897743 3.770480590734768E-6 0.0 82-83 0.9572609238175152 3.770480590734768E-6 0.0 84-85 1.150780840136977 3.770480590734768E-6 0.0 86-87 1.379811142659979 3.770480590734768E-6 0.0 88-89 1.6474851007574216 3.770480590734768E-6 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 438410 0.0 60.048203 1 TAACTTT 69995 0.0 59.116535 1 TTTGTGT 465255 0.0 56.55659 2 AACTTTG 74485 0.0 56.06245 2 TTGTGTT 521465 0.0 51.77232 3 ACTTTGT 151610 0.0 50.718105 1 TGTGTTT 548575 0.0 49.4648 4 GTGTTTG 603250 0.0 45.02209 5 TTGAGGG 84570 0.0 34.810654 9 TTTGAGG 170060 0.0 34.139294 8 GTTTGAG 451255 0.0 33.968407 7 TGTTTGA 807165 0.0 33.668873 6 TTGAGGC 26725 0.0 31.412416 9 GTTTGAC 168910 0.0 30.599186 7 TTTGACC 55140 0.0 29.958803 8 TTGACCC 30620 0.0 29.355011 9 TTTGAGC 111885 0.0 28.930653 8 TTGAGCC 50830 0.0 28.61302 9 TTTGACG 47055 0.0 27.901411 8 TCCGATC 1945 0.0 27.586441 7 >>END_MODULE