FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l04n01_6957.34100000035bda.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l04n01_6957.34100000035bda.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26521818
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG4384100.060.0482031
TAACTTT699950.059.1165351
TTTGTGT4652550.056.556592
AACTTTG744850.056.062452
TTGTGTT5214650.051.772323
ACTTTGT1516100.050.7181051
TGTGTTT5485750.049.46484
GTGTTTG6032500.045.022095
TTGAGGG845700.034.8106549
TTTGAGG1700600.034.1392948
GTTTGAG4512550.033.9684077
TGTTTGA8071650.033.6688736
TTGAGGC267250.031.4124169
GTTTGAC1689100.030.5991867
TTTGACC551400.029.9588038
TTGACCC306200.029.3550119
TTTGAGC1118850.028.9306538
TTGAGCC508300.028.613029
TTTGACG470550.027.9014118
TCCGATC19450.027.5864417
Produced by FastQC (version 0.11.2)