##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l04n01_6929.34100000035b97.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15584665 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.73162137267628 33.0 33.0 33.0 33.0 33.0 2 32.765189883773566 33.0 33.0 33.0 33.0 33.0 3 32.79261177574237 33.0 33.0 33.0 33.0 33.0 4 36.60359802408329 37.0 37.0 37.0 37.0 37.0 5 36.62781554816866 37.0 37.0 37.0 37.0 37.0 6 36.6395183983743 37.0 37.0 37.0 37.0 37.0 7 36.620348015180305 37.0 37.0 37.0 37.0 37.0 8 36.572509835790505 37.0 37.0 37.0 37.0 37.0 9 36.59837359352928 37.0 37.0 37.0 37.0 37.0 10-11 36.590751678011685 37.0 37.0 37.0 37.0 37.0 12-13 36.57245150922397 37.0 37.0 37.0 37.0 37.0 14-15 38.67643022804789 40.0 37.0 40.0 37.0 40.0 16-17 38.65358742071132 40.0 37.0 40.0 37.0 40.0 18-19 38.624840732861436 40.0 37.0 40.0 37.0 40.0 20-21 38.57361621183388 40.0 37.0 40.0 37.0 40.0 22-23 38.5255046226531 40.0 37.0 40.0 37.0 40.0 24-25 38.48372862040987 40.0 37.0 40.0 37.0 40.0 26-27 38.39037441613279 40.0 37.0 40.0 37.0 40.0 28-29 38.338822233265844 40.0 37.0 40.0 37.0 40.0 30-31 38.28229070050591 40.0 37.0 40.0 37.0 40.0 32-33 38.188185950740674 40.0 37.0 40.0 37.0 40.0 34-35 38.11335062383439 40.0 37.0 40.0 37.0 40.0 36-37 38.039739577334515 40.0 37.0 40.0 37.0 40.0 38-39 37.92743328136986 40.0 37.0 40.0 35.0 40.0 40-41 37.770591924818405 40.0 37.0 40.0 33.0 40.0 42-43 37.67176169651385 40.0 37.0 40.0 33.0 40.0 44-45 37.55582802068572 40.0 37.0 40.0 33.0 40.0 46-47 37.39555736360069 40.0 37.0 40.0 33.0 40.0 48-49 37.20444921337738 37.0 37.0 40.0 33.0 40.0 50-51 37.01564765748895 37.0 37.0 40.0 33.0 40.0 52-53 36.780971037876014 37.0 37.0 40.0 33.0 40.0 54-55 36.524038084873816 37.0 37.0 40.0 33.0 40.0 56-57 36.24997165482864 37.0 37.0 40.0 33.0 40.0 58-59 36.00480915053355 37.0 37.0 40.0 33.0 40.0 60-61 35.7221349961645 37.0 35.0 37.0 33.0 40.0 62-63 35.404770266155865 37.0 33.0 37.0 33.0 40.0 64-65 35.17673992350814 37.0 33.0 37.0 33.0 40.0 66-67 34.91421320252954 37.0 33.0 37.0 30.0 40.0 68-69 34.6632431303464 37.0 33.0 37.0 27.0 38.5 70-71 34.403171065916396 37.0 33.0 37.0 27.0 37.0 72-73 34.180139066191025 37.0 33.0 37.0 27.0 37.0 74-75 33.951474542442845 37.0 33.0 37.0 27.0 37.0 76-77 31.159730382398337 33.0 30.0 35.0 24.5 37.0 78-79 33.67863595399709 35.0 33.0 37.0 27.0 37.0 80-81 34.13014132802983 37.0 33.0 37.0 27.0 37.0 82-83 34.12246791958634 37.0 33.0 37.0 27.0 37.0 84-85 34.06091414220325 37.0 33.0 37.0 27.0 37.0 86-87 33.95517866441146 37.0 33.0 37.0 27.0 37.0 88-89 33.776668282571364 37.0 33.0 37.0 27.0 37.0 90-91 33.65036787765409 37.0 33.0 37.0 27.0 37.0 92-93 33.5462431178341 37.0 33.0 37.0 27.0 37.0 94-95 33.44652839826843 37.0 33.0 37.0 27.0 37.0 96-97 33.33214034437057 37.0 33.0 37.0 27.0 37.0 98-99 33.18786451296836 33.0 33.0 37.0 27.0 37.0 100-101 31.323432714145603 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1295972861408643 2306 2 0.11724379192938983 2306 3 0.08436484245074638 2306 4 -0.15795448504141518 2306 5 -0.050337219218768325 2306 6 0.079272541008379 2306 7 0.12124388909045081 2306 8 0.17131948118728957 2306 9 0.15451776042778675 2306 10-11 0.11632504020801804 2306 12-13 0.09466810372183687 2306 14-15 0.20718294467847898 2306 16-17 0.20874456822304666 2306 18-19 0.1535366154823521 2306 20-21 0.1885485088906691 2306 22-23 0.19893507420130163 2306 24-25 0.19877811198348638 2306 26-27 0.21591893191290268 2306 28-29 0.14803019075841206 2306 30-31 0.18800423349242124 2306 32-33 0.15920749391298017 2306 34-35 0.14864434683418182 2306 36-37 0.12205063848122677 2306 38-39 0.13612923634222085 2306 40-41 0.2240740421291889 2306 42-43 0.13713787944822542 2306 44-45 0.10200552452744915 2306 46-47 0.09963996093650707 2306 48-49 0.08579335333985938 2306 50-51 0.046546973052159046 2306 52-53 0.08428643011085768 2306 54-55 0.12213334971211509 2306 56-57 0.0645365124238424 2306 58-59 -0.027171698665561905 2306 60-61 -0.05013462533783297 2306 62-63 -0.01258498400978425 2306 64-65 -0.09737363919806796 2306 66-67 -0.09534697640778944 2306 68-69 -0.27485015864096596 2306 70-71 -0.25120623757990757 2306 72-73 -0.1341451931998705 2306 74-75 -0.270654433055995 2306 76-77 -0.8413140682784288 2306 78-79 -0.18809667898624127 2306 80-81 0.06708472056973847 2306 82-83 -0.07987194289311361 2306 84-85 -0.1789943113717598 2306 86-87 -0.10820836419506463 2306 88-89 -0.16502419266678459 2306 90-91 -0.2038487313053423 2306 92-93 -0.261220932791808 2306 94-95 -0.340635391453354 2306 96-97 -0.19278712263689357 2306 98-99 -0.12428029634074278 2306 100-101 -0.4572741733468213 2307 1 0.12847538534415293 2307 2 0.09717202410944736 2307 3 0.07465009504945641 2307 4 -0.1084058504856813 2307 5 0.010367817884997521 2307 6 0.08520928322538879 2307 7 0.10264034832361801 2307 8 0.16386467929208948 2307 9 0.11329808130839325 2307 10-11 0.10620058202825788 2307 12-13 0.0734013342700095 2307 14-15 0.15628476142901349 2307 16-17 0.1426168957340863 2307 18-19 0.12070991308821988 2307 20-21 0.13263116821065069 2307 22-23 0.129212687994972 2307 24-25 0.15655235246727273 2307 26-27 0.15830499336508552 2307 28-29 0.13527514006791108 2307 30-31 0.1828965542317107 2307 32-33 0.16263012326012216 2307 34-35 0.21291856330436332 2307 36-37 0.1471165125744207 2307 38-39 0.09648049102426626 2307 40-41 0.15917250917141246 2307 42-43 0.07564433394558279 2307 44-45 0.11874340582597398 2307 46-47 0.07124468016400698 2307 48-49 0.10055877438541927 2307 50-51 0.011559428548991946 2307 52-53 -0.012845577958820797 2307 54-55 0.004022461977982061 2307 56-57 -0.10963898892723734 2307 58-59 -0.08675802255258702 2307 60-61 -0.15103642055365896 2307 62-63 -0.13230710165033344 2307 64-65 -0.24705204908664768 2307 66-67 -0.2772237159190638 2307 68-69 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24-25 0.11612581856589799 2201 26-27 0.12806847402242028 2201 28-29 0.10945143377650624 2201 30-31 0.10532160629511367 2201 32-33 0.1568513360475592 2201 34-35 0.054804991503608846 2201 36-37 0.03784751570601941 2201 38-39 0.08042975884743697 2201 40-41 0.16957284085384572 2201 42-43 0.054101574437844135 2201 44-45 0.006381685024329897 2201 46-47 -0.012838548580873521 2201 48-49 0.02765162988372083 2201 50-51 0.04573397728951534 2201 52-53 0.021536455794539222 2201 54-55 0.0383853634028668 2201 56-57 -0.03733973729002571 2201 58-59 -0.04299982527079038 2201 60-61 -0.07662967334378834 2201 62-63 0.032042054688766086 2201 64-65 -0.06971067759021565 2201 66-67 -0.1404570413469557 2201 68-69 -0.20137145266594558 2201 70-71 -0.1730950786060319 2201 72-73 -0.13261264341408463 2201 74-75 -0.20461769728069612 2201 76-77 -0.8431216695667594 2201 78-79 -0.11035182878968897 2201 80-81 -0.02470782377291414 2201 82-83 -0.015343583602742683 2201 84-85 -0.13306379248929545 2201 86-87 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0.18014879058114985 1101 44-45 0.1653711564987006 1101 46-47 0.13904048470326558 1101 48-49 0.09550643801644298 1101 50-51 0.027454338376593057 1101 52-53 -0.06299962110504254 1101 54-55 0.053022234235989174 1101 56-57 0.05624628489938743 1101 58-59 0.013617318095285214 1101 60-61 -0.013527206297119676 1101 62-63 0.01988268271054494 1101 64-65 -0.1071171168492171 1101 66-67 -0.14549389436746907 1101 68-69 -0.10729538645392722 1101 70-71 -0.17355728406177917 1101 72-73 -0.025444657765959278 1101 74-75 -0.22922625878510416 1101 76-77 -0.6983440981890823 1101 78-79 0.022000759128268044 1101 80-81 0.05027179987205699 1101 82-83 0.06389425292837814 1101 84-85 -0.005003248695715001 1101 86-87 -0.0026209269108576905 1101 88-89 0.07209590341025063 1101 90-91 0.05543785032853066 1101 92-93 -0.03092864666640338 1101 94-95 -0.059709060114435886 1101 96-97 0.07907247027234376 1101 98-99 -0.022491894844925753 1101 100-101 -0.29177059658374915 2304 1 0.0957793085604024 2304 2 0.0822929244297228 2304 3 0.06887358900218032 2304 4 -0.16305794791630746 2304 5 -0.047667701438655286 2304 6 0.05966893908874482 2304 7 0.06872821207527835 2304 8 0.132181375411065 2304 9 0.07030147969975076 2304 10-11 0.057513897643119094 2304 12-13 0.06457261836130357 2304 14-15 0.07428829037817763 2304 16-17 0.08851075868410163 2304 18-19 0.09326244047034038 2304 20-21 0.09889015638146503 2304 22-23 0.09957766637548104 2304 24-25 0.07988713477263332 2304 26-27 0.11946586169207052 2304 28-29 0.07373773712607345 2304 30-31 0.05779125500855997 2304 32-33 0.09282690057980858 2304 34-35 0.0896992545404558 2304 36-37 0.06495706784961186 2304 38-39 0.026093919194892123 2304 40-41 0.078815860218171 2304 42-43 0.13410695231620195 2304 44-45 0.05923259845483386 2304 46-47 0.04592746029781125 2304 48-49 -0.025413107624238762 2304 50-51 -0.09752105531501343 2304 52-53 -0.02477162460504445 2304 54-55 0.04949981423816752 2304 56-57 -0.028907282007764934 2304 58-59 -0.061721568899820056 2304 60-61 -0.06877350259462389 2304 62-63 -0.0026858329068630837 2304 64-65 0.021865162855888798 2304 66-67 -0.010923299128016595 2304 68-69 -0.1510133140467005 2304 70-71 -0.12854167599919464 2304 72-73 -0.027478936600012105 2304 74-75 -0.09929638125709062 2304 76-77 -0.30517053439796626 2304 78-79 0.05663306156965575 2304 80-81 0.07786968467008393 2304 82-83 0.03247714564611215 2304 84-85 -0.005571437347882124 2304 86-87 0.06143567194569499 2304 88-89 0.04555281485928475 2304 90-91 0.03201302935173089 2304 92-93 -0.040796662099481296 2304 94-95 0.06337387390809823 2304 96-97 0.07626511629785426 2304 98-99 0.032092341700668214 2304 100-101 -0.19642859902493015 2202 1 0.09846542696688942 2202 2 0.07279632246211776 2202 3 0.035790869772554856 2202 4 -0.18205481973702575 2202 5 -0.06273353578859542 2202 6 0.034556147844654106 2202 7 0.04149045663878326 2202 8 0.1601138401948461 2202 9 0.07519161544980335 2202 10-11 0.058029988495505336 2202 12-13 0.04951734397206309 2202 14-15 0.0736341590311298 2202 16-17 0.05258806078027334 2202 18-19 0.07462526075633491 2202 20-21 0.08930817926940904 2202 22-23 0.08150521464377647 2202 24-25 0.08728953008042595 2202 26-27 0.08093108880366628 2202 28-29 0.07738024592276105 2202 30-31 0.1048684875332242 2202 32-33 0.07458750358707533 2202 34-35 0.05617603140402849 2202 36-37 0.06547540138273433 2202 38-39 0.0462144574219181 2202 40-41 0.11363287349750806 2202 42-43 0.10476726453436669 2202 44-45 0.03496092811396778 2202 46-47 0.07828656003564305 2202 48-49 0.09742908931397665 2202 50-51 0.016282837109621084 2202 52-53 0.016578610746286415 2202 54-55 0.054526089313483794 2202 56-57 0.07252588939793014 2202 58-59 0.06964131162124687 2202 60-61 -0.03253288721851533 2202 62-63 0.01932067073818189 2202 64-65 0.020769841890306395 2202 66-67 0.07398299059217806 2202 68-69 -0.017110221130756997 2202 70-71 -0.085671462439187 2202 72-73 -0.05181868601524542 2202 74-75 -0.11667423658226994 2202 76-77 -0.08630409247838955 2202 78-79 0.09082458205082133 2202 80-81 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36-37 0.024470856934399876 1102 38-39 -0.008245740494487563 1102 40-41 0.0413267156117314 1102 42-43 0.043130139095190145 1102 44-45 -0.04208322103210094 1102 46-47 -0.018968465647581922 1102 48-49 -0.020356374258014398 1102 50-51 -0.028487032383452515 1102 52-53 -0.04626102277385513 1102 54-55 -0.05132975742807133 1102 56-57 -0.007921728958613983 1102 58-59 -0.1253601188594331 1102 60-61 -0.0609980782850883 1102 62-63 -0.02667778201207227 1102 64-65 -0.037297263811076675 1102 66-67 -0.047036719516690084 1102 68-69 -0.17156417840488558 1102 70-71 -0.1983372130626293 1102 72-73 -0.15983039302862068 1102 74-75 -0.24440741716395564 1102 76-77 -0.2393443551157155 1102 78-79 -0.05048612175482248 1102 80-81 0.0059874785548359455 1102 82-83 -0.09804821045936052 1102 84-85 -0.07855503434443989 1102 86-87 -0.04842940073218216 1102 88-89 -0.12688998191445222 1102 90-91 -0.18316212921041597 1102 92-93 -0.19492981173517165 1102 94-95 -0.325190241854564 1102 96-97 -0.2823752719837884 1102 98-99 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52-53 -0.2819556232069331 1213 54-55 -0.18305659594830814 1213 56-57 -0.16995035253600577 1213 58-59 -0.20131567241501358 1213 60-61 -0.20467356925892943 1213 62-63 -0.055683495515069126 1213 64-65 -0.054691251754647396 1213 66-67 -0.010479940225650353 1213 68-69 0.05466338943316629 1213 70-71 -0.06946507242067668 1213 72-73 -0.013921808810671621 1213 74-75 0.043103168109873025 1213 76-77 0.514020268061536 1213 78-79 -0.018889113185778683 1213 80-81 -0.1955160613671012 1213 82-83 -0.2887346433738003 1213 84-85 -0.19434042765924175 1213 86-87 -0.19454163624127574 1213 88-89 -0.08809730079336475 1213 90-91 -0.05285360443343734 1213 92-93 0.04194494292169537 1213 94-95 -0.0014958326830765145 1213 96-97 0.029428812016938366 1213 98-99 0.09073373125621487 1213 100-101 0.3564562116121728 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 6.0 7 47.0 8 502.0 9 1375.0 10 942.0 11 343.0 12 251.0 13 402.0 14 988.0 15 2298.0 16 4202.0 17 6497.0 18 9332.0 19 12522.0 20 16836.0 21 22076.0 22 28241.0 23 36904.0 24 47624.0 25 61739.0 26 79802.0 27 102061.0 28 131065.0 29 170307.0 30 221449.0 31 293098.0 32 399824.0 33 579250.0 34 928241.0 35 1771965.0 36 3910125.0 37 5566247.0 38 1177946.0 39 158.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 20.936812830107364 24.525992308098047 29.8008808238326 24.73631403796199 2 26.070249184053683 25.003797001732153 28.396580869720328 20.529372944493833 3 21.828470486853583 22.87061030827419 33.066697295065374 22.234221909806852 4 21.7262678937606 23.614464952670485 31.98661499575819 22.67265215781072 5 26.091930753724895 22.81610801387133 29.76044079227882 21.331520440124958 6 27.360799863198853 23.32463354201069 28.51277842674193 20.801788168048528 7 25.911734387617567 26.9767300099168 25.70981795245519 21.401717650010443 8 25.079666454171456 24.455905853606737 28.474959198673826 21.989468493547985 9 22.843134581333636 23.38238903434883 31.133733063880424 22.64074332043711 10-11 25.442467903457512 24.231568969271486 27.62550858098522 22.70045454628578 12-13 24.940130416122763 26.091091343677736 25.85413474459029 23.11464349560921 14-15 24.647099374330413 25.068446296578685 26.802834081082338 23.481620248008564 16-17 24.348980231528877 25.020184906124065 26.565312760973686 24.065522101373368 18-19 24.27038053111825 25.18832454852254 26.841953291905856 23.699341628453354 20-21 23.796192973844924 25.455733287758214 27.026663273985257 23.72141046441161 22-23 23.965248408526104 25.658515035024937 26.553927239757684 23.822309316691275 24-25 24.252949935080416 25.538688191244407 26.308345415188583 23.900016458486594 26-27 24.128247222510076 25.598419985286817 26.497139977022282 23.77619281518082 28-29 24.09659110413987 25.90821811055932 26.250711837565966 23.74447894773484 30-31 24.166576567414186 25.810676713294768 26.157514454112423 23.865232265178623 32-33 24.132408364247805 26.08355713773764 26.06434273691478 23.719691761099774 34-35 23.970941948383235 26.04335415615286 26.153404644886496 23.83229925057741 36-37 24.060164825557425 25.877172972509932 26.212021607567333 23.85064059436531 38-39 24.161859351359276 25.801801028289972 26.186790525542907 23.84954909480784 40-41 23.872223735743706 26.035530240335696 26.08349413006245 24.00875189385815 42-43 23.92519781352391 25.873455490453217 26.07012604721858 24.131220648804298 44-45 24.11404094986963 25.791443062779983 26.112078764606107 23.98243722274428 46-47 23.92738952040355 25.876603699854954 26.130584135109736 24.06542264463176 48-49 23.965385071207507 25.665704160558366 26.07398376211279 24.294927006121338 50-51 23.920527093587523 25.82502426612731 26.287955947971575 23.96649269231359 52-53 23.98436603826316 25.8457104068812 26.33710978667587 23.83281376817977 54-55 23.873139283928484 25.897348229329342 26.054160852731084 24.175351634011086 56-57 23.988056290255724 26.01211536824551 26.1730732881372 23.82675505336157 58-59 23.844178920078317 26.15878912127173 26.184012659707097 23.81301929894286 60-61 24.179532244796338 25.989077412921528 25.845858823861573 23.985531518420554 62-63 23.951799550666163 26.129615618314627 26.19638654798689 23.72219828303232 64-65 24.039129995650697 26.064404977738214 26.087171763333743 23.809293263277343 66-67 24.13254881141091 26.06723257086442 26.153285184213203 23.646933433511467 68-69 24.06473848463481 25.91952495614846 26.33457991441289 23.681156644803846 70-71 23.964450775351605 26.15977177670164 26.145237775016028 23.73053967293073 72-73 23.897302321903855 25.939342546536654 26.248416732042518 23.91493839951697 74-75 23.97976910094001 25.965529250759552 26.218935250118193 23.835766398182248 76-77 24.00202459373117 25.98717635371041 26.074727133403293 23.936071919155125 78-79 23.9200306632054 25.91276682552274 26.054400623018015 24.11280188825384 80-81 23.93895729840742 25.92980743840817 26.161111073679184 23.97012418950522 82-83 23.78710014301447 26.309721374446855 26.142576522366163 23.760601960172515 84-85 23.908396743759415 26.137465912641193 25.968842399728242 23.98529494387115 86-87 23.851448245416357 26.236910096602315 26.022971660802156 23.888669997179168 88-89 23.910404956275144 26.784559385522506 25.666147072356665 23.638888585845685 90-91 23.89158690687219 26.254301829879328 25.780420578436157 24.073690684812323 92-93 23.798196055109525 26.439017766160873 25.699940006265376 24.062846172464226 94-95 23.87951633801995 26.641810223019718 25.539057289046692 23.93961614991364 96-97 23.845221747853966 26.387341047758557 25.593823942234405 24.17361326215307 98-99 24.090981359037823 26.358732194456657 25.502147326026005 24.04813912047952 100-101 24.220689367129683 26.73818860007743 25.18832538436948 23.852796648423407 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2032.0 1 1536.0 2 1154.0 3 1227.5 4 1251.5 5 1286.5 6 1278.0 7 1353.5 8 1453.0 9 1580.0 10 1738.0 11 1950.0 12 2213.5 13 2469.0 14 2768.0 15 3120.0 16 3457.5 17 3789.0 18 4287.0 19 4970.5 20 5711.0 21 6674.5 22 7745.0 23 9204.0 24 11329.5 25 14158.0 26 17780.0 27 22017.5 28 27168.5 29 33981.5 30 43978.0 31 57402.0 32 75223.5 33 97479.5 34 125800.5 35 160715.5 36 198631.0 37 241814.0 38 286434.0 39 330993.0 40 381357.0 41 439903.0 42 495760.5 43 532922.5 44 581154.0 45 679255.5 46 784407.5 47 868448.5 48 947018.5 49 971140.0 50 933734.0 51 882555.0 52 811003.5 53 749378.0 54 685617.0 55 620534.0 56 578626.0 57 478001.0 58 353098.5 59 269610.5 60 206112.5 61 157178.5 62 116997.0 63 81991.0 64 54264.0 65 33774.0 66 20673.0 67 13474.5 68 10053.5 69 7837.5 70 5963.5 71 4359.0 72 3153.0 73 2302.0 74 1626.0 75 1084.0 76 709.5 77 462.0 78 328.0 79 235.5 80 146.0 81 98.5 82 66.5 83 35.0 84 28.5 85 18.5 86 7.5 87 6.0 88 6.5 89 4.0 90 2.0 91 2.5 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12489200120759734 2 0.0 3 0.0 4 0.3436262505482152 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.2457973912175846E-5 12-13 0.0010619413378471722 14-15 9.624845962361077E-6 16-17 0.0 18-19 0.0 20-21 8.886941105246728E-4 22-23 3.5291101861990615E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0019891348322212893 38-39 0.007205801343820993 40-41 0.006843265479238726 42-43 0.0014597683042914301 44-45 0.0 46-47 0.0 48-49 1.2833127949814769E-5 50-51 0.0011806477713829588 52-53 0.012335844241759448 54-55 0.009092271152443765 56-57 0.008979981282882885 58-59 0.012820294821864954 60-61 0.0028553709688337865 62-63 2.4703771303393433E-4 64-65 0.0035868592619732282 66-67 0.011270694621924822 68-69 0.019981180217861598 70-71 0.02540638505864579 72-73 0.019050778441500025 74-75 0.003737648515383552 76-77 0.014626557580801385 78-79 0.007488130158716918 80-81 0.005261582459424056 82-83 0.003705565695509015 84-85 0.011071781138702693 86-87 0.015261797414317215 88-89 0.012801045129940233 90-91 0.03087971412924179 92-93 0.014145315282683328 94-95 0.014459726917453791 96-97 0.00726355041959516 98-99 0.012271678602010375 100-101 0.1401377572119773 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5584665E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.96690330455089 #Duplication Level Percentage of deduplicated Percentage of total 1 81.96760335390312 29.481208639356147 2 9.264933554129529 6.664619385289315 3 2.6019662647233583 2.807540071350255 4 1.5200682575953115 2.1868859214899095 5 0.8574496449490374 1.5419904234201762 6 0.6371657540317847 1.3750127438539475 7 0.33252380533433884 0.837189608704503 8 0.2785636557294121 0.8015257655825576 9 0.17744152067656677 0.5743819814747889 >10 1.7154288283006018 12.475889519053549 >50 0.29726905096233414 7.573371401927899 >100 0.31674304666828257 22.5316289134985 >500 0.02301057031963228 5.622815577772731 >1k 0.009832692676677413 5.525940047225818 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.699876769888861E-5 0.0 0.0 2 7.699876769888861E-5 0.0 0.0 3 7.699876769888861E-5 0.0 0.0 4 8.3415331673796E-5 0.0 0.0 5 8.3415331673796E-5 0.0 0.0 6 8.3415331673796E-5 1.2833127949814769E-5 0.0 7 1.0266502359851815E-4 1.2833127949814769E-5 0.0 8 1.1549815154833292E-4 1.2833127949814769E-5 0.0 9 1.4116440744796246E-4 1.2833127949814769E-5 0.0 10-11 2.021217652095826E-4 1.2833127949814769E-5 0.0 12-13 2.5987084098374905E-4 1.2833127949814769E-5 0.0 14-15 3.849938384944431E-4 1.2833127949814769E-5 3.2082819874536923E-6 16-17 5.069085540176834E-4 1.2833127949814769E-5 9.624845962361077E-6 18-19 7.218634471770808E-4 1.2833127949814769E-5 1.2833127949814769E-5 20-21 9.528597502737467E-4 1.2833127949814769E-5 1.2833127949814769E-5 22-23 0.0012319802831822178 1.604140993726846E-5 1.2833127949814769E-5 24-25 0.001562433327889948 1.9249691924722154E-5 1.2833127949814769E-5 26-27 0.001950635448371845 1.9249691924722154E-5 1.2833127949814769E-5 28-29 0.0023003381850042973 1.9249691924722154E-5 1.2833127949814769E-5 30-31 0.0027591225092101755 1.9249691924722154E-5 1.2833127949814769E-5 32-33 0.003298113883102396 2.5666255899629538E-5 1.2833127949814769E-5 34-35 0.003904479178731144 3.208281987453692E-5 1.2833127949814769E-5 36-37 0.004690508265657298 3.208281987453692E-5 1.2833127949814769E-5 38-39 0.005675450835805582 3.208281987453692E-5 1.2833127949814769E-5 40-41 0.006843265479238726 4.1707665836898E-5 1.2833127949814769E-5 42-43 0.008165077658069647 4.491594782435169E-5 1.2833127949814769E-5 44-45 0.00968901160211015 4.491594782435169E-5 1.2833127949814769E-5 46-47 0.011793644585879774 4.491594782435169E-5 1.2833127949814769E-5 48-49 0.014251188588269303 5.774907577416646E-5 1.2833127949814769E-5 50-51 0.017459470575722995 5.774907577416646E-5 1.2833127949814769E-5 52-53 0.021370366318429045 5.774907577416646E-5 1.604140993726846E-5 54-55 0.026182789299609585 5.774907577416646E-5 2.2457973912175846E-5 56-57 0.03232985758757086 5.774907577416646E-5 2.5666255899629538E-5 58-59 0.03964794880095274 5.774907577416646E-5 2.5666255899629538E-5 60-61 0.04897442453848062 5.774907577416646E-5 3.208281987453692E-5 62-63 0.06061727987095006 6.095735776162015E-5 3.208281987453692E-5 64-65 0.07513154758219057 6.416563974907385E-5 3.208281987453692E-5 66-67 0.0936208766758862 6.416563974907385E-5 3.208281987453692E-5 68-69 0.11722420725758301 6.416563974907385E-5 3.208281987453692E-5 70-71 0.1465382797769474 6.416563974907385E-5 3.208281987453692E-5 72-73 0.18285282359293575 7.379048571143492E-5 3.5291101861990615E-5 74-75 0.2283751367129162 7.699876769888861E-5 3.849938384944431E-5 76-77 0.2847510677964525 7.699876769888861E-5 3.849938384944431E-5 78-79 0.35634067206449416 8.020704968634231E-5 4.1707665836898E-5 80-81 0.4428006633443837 8.3415331673796E-5 4.491594782435169E-5 82-83 0.5486932186222803 8.3415331673796E-5 4.491594782435169E-5 84-85 0.678163438225974 8.662361366124969E-5 4.491594782435169E-5 86-87 0.8353307562273555 9.624845962361077E-5 4.491594782435169E-5 88-89 1.024093235241181 9.624845962361077E-5 4.491594782435169E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 73130 0.0 67.10265 1 TTTGTGT 80115 0.0 61.312443 2 TTGTGTT 91405 0.0 55.15191 3 TGTGTTT 101345 0.0 50.005825 4 ACTTTGT 22715 0.0 49.645657 1 GTGTTTG 109860 0.0 46.064167 5 GTTTGAG 89595 0.0 34.102104 2 TGTTTGA 176410 0.0 33.57145 1 TTTGAGG 33395 0.0 30.271395 8 AACTTTG 11180 0.0 29.489542 2 TTTGAGT 39875 0.0 28.544289 3 TAACTTT 11600 0.0 28.375118 1 TTGAGGG 18010 0.0 27.050287 9 TTTGAGC 27630 0.0 23.968325 3 GTTTGAC 41930 0.0 23.088865 2 TTGAGTC 16325 0.0 22.851376 4 GTTTGAA 22185 0.0 22.764084 2 GTTTGAT 63505 0.0 22.62701 2 TTGAGGC 8410 0.0 21.980597 4 GAGTGGG 3290 0.0 19.628235 6 >>END_MODULE