##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l04n01_6777.34100000035b47.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12718229 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.733894239520296 33.0 33.0 33.0 33.0 33.0 2 32.769427881822224 33.0 33.0 33.0 33.0 33.0 3 32.7977794707109 33.0 33.0 33.0 33.0 33.0 4 36.61186561430841 37.0 37.0 37.0 37.0 37.0 5 36.63442999807599 37.0 37.0 37.0 37.0 37.0 6 36.64451465687558 37.0 37.0 37.0 37.0 37.0 7 36.624507390140565 37.0 37.0 37.0 37.0 37.0 8 36.57630720440716 37.0 37.0 37.0 37.0 37.0 9 36.60325333031824 37.0 37.0 37.0 37.0 37.0 10-11 36.597086630536374 37.0 37.0 37.0 37.0 37.0 12-13 36.57607077211772 37.0 37.0 37.0 37.0 37.0 14-15 38.6735612717777 40.0 37.0 40.0 37.0 40.0 16-17 38.652411904204584 40.0 37.0 40.0 37.0 40.0 18-19 38.62333615788802 40.0 37.0 40.0 37.0 40.0 20-21 38.56938753029215 40.0 37.0 40.0 37.0 40.0 22-23 38.515215286656655 40.0 37.0 40.0 37.0 40.0 24-25 38.470905383131566 40.0 37.0 40.0 37.0 40.0 26-27 38.37431135262622 40.0 37.0 40.0 37.0 40.0 28-29 38.320026278816016 40.0 37.0 40.0 37.0 40.0 30-31 38.26711030285742 40.0 37.0 40.0 37.0 40.0 32-33 38.177110704642914 40.0 37.0 40.0 37.0 40.0 34-35 38.101773682483625 40.0 37.0 40.0 37.0 40.0 36-37 38.02443559555344 40.0 37.0 40.0 37.0 40.0 38-39 37.905128614998205 40.0 37.0 40.0 33.0 40.0 40-41 37.74843478600676 40.0 37.0 40.0 33.0 40.0 42-43 37.644868519036734 40.0 37.0 40.0 33.0 40.0 44-45 37.530827405293614 40.0 37.0 40.0 33.0 40.0 46-47 37.37416255046202 40.0 37.0 40.0 33.0 40.0 48-49 37.18047402669035 37.0 37.0 40.0 33.0 40.0 50-51 36.985901653445616 37.0 37.0 40.0 33.0 40.0 52-53 36.737285670827276 37.0 37.0 40.0 33.0 40.0 54-55 36.472860490245935 37.0 37.0 40.0 33.0 40.0 56-57 36.19640438932181 37.0 37.0 40.0 33.0 40.0 58-59 35.94846959431223 37.0 37.0 40.0 33.0 40.0 60-61 35.65777758837335 37.0 33.0 37.0 33.0 40.0 62-63 35.34552467957606 37.0 33.0 37.0 33.0 40.0 64-65 35.11522893635584 37.0 33.0 37.0 33.0 40.0 66-67 34.84683185842935 37.0 33.0 37.0 27.0 40.0 68-69 34.59805272416466 37.0 33.0 37.0 27.0 37.0 70-71 34.33507868902188 37.0 33.0 37.0 27.0 37.0 72-73 34.11680608204177 37.0 33.0 37.0 27.0 37.0 74-75 33.888916491439176 37.0 33.0 37.0 27.0 37.0 76-77 31.08451719181971 33.0 30.0 35.0 24.5 37.0 78-79 33.62519734469319 35.0 33.0 37.0 27.0 37.0 80-81 34.08049351839789 37.0 33.0 37.0 27.0 37.0 82-83 34.072929611504875 37.0 33.0 37.0 27.0 37.0 84-85 34.01662562452681 37.0 33.0 37.0 27.0 37.0 86-87 33.90744383514403 37.0 33.0 37.0 27.0 37.0 88-89 33.72079426310063 37.0 33.0 37.0 27.0 37.0 90-91 33.593539084726345 37.0 33.0 37.0 27.0 37.0 92-93 33.48426298189787 37.0 33.0 37.0 27.0 37.0 94-95 33.37819813591971 37.0 33.0 37.0 27.0 37.0 96-97 33.269646465714686 37.0 33.0 37.0 27.0 37.0 98-99 33.12513043286137 33.0 33.0 37.0 27.0 37.0 100-101 31.23860079103781 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.12478852661314477 2306 2 0.11037046285771623 2306 3 0.09469993339035909 2306 4 -0.06758561006547836 2306 5 0.024152638613365696 2306 6 0.07818364285733281 2306 7 0.09190875757353467 2306 8 0.1682543684736757 2306 9 0.13279849060832305 2306 10-11 0.1241291017734909 2306 12-13 0.13304295678720024 2306 14-15 0.22458853919323474 2306 16-17 0.21793176933637426 2306 18-19 0.15610818229988155 2306 20-21 0.16332605528141642 2306 22-23 0.17470503236854285 2306 24-25 0.18574248740544164 2306 26-27 0.21554966279275334 2306 28-29 0.1465602179574219 2306 30-31 0.2075237102915608 2306 32-33 0.20887757229414916 2306 34-35 0.15889472345455147 2306 36-37 0.14670792126967314 2306 38-39 0.1618782875437219 2306 40-41 0.2561876603267379 2306 42-43 0.17894163279104447 2306 44-45 0.12922416456245145 2306 46-47 0.12705615779255197 2306 48-49 0.09683092257029102 2306 50-51 0.07590333440902697 2306 52-53 0.11826345267056126 2306 54-55 0.10519340056042381 2306 56-57 0.018121848166039456 2306 58-59 0.003813549576527464 2306 60-61 -0.07838729328561556 2306 62-63 -0.007467536366938532 2306 64-65 -0.03121012387581601 2306 66-67 -0.12333664134484934 2306 68-69 -0.1379248124853163 2306 70-71 -0.18389621113639976 2306 72-73 -0.13010458714875028 2306 74-75 -0.24081191817026593 2306 76-77 -0.7988462102803204 2306 78-79 -0.17389580930078097 2306 80-81 0.03976795297137414 2306 82-83 -0.08880917164349711 2306 84-85 -0.18499112118391992 2306 86-87 -0.13404915921489646 2306 88-89 -0.13826959624518054 2306 90-91 -0.14460467398817656 2306 92-93 -0.2593919047879183 2306 94-95 -0.29667373347195536 2306 96-97 -0.18497688591789796 2306 98-99 -0.08268459312683518 2306 100-101 -0.3745764430327654 2307 1 0.11671198576626551 2307 2 0.08259785361484973 2307 3 0.07830768633986906 2307 4 -0.16097741613517513 2307 5 -0.026952757734783006 2307 6 0.07465768379876181 2307 7 0.08079029269547533 2307 8 0.15643318543031626 2307 9 0.10626437854977411 2307 10-11 0.09659828613666832 2307 12-13 0.075647488100131 2307 14-15 0.16201028546883123 2307 16-17 0.14672756403511045 2307 18-19 0.11385188282608993 2307 20-21 0.1554770579180058 2307 22-23 0.12893805286770288 2307 24-25 0.12344633599516186 2307 26-27 0.16393086219715514 2307 28-29 0.13100909065544641 2307 30-31 0.13383092073522818 2307 32-33 0.10785461501082949 2307 34-35 0.1451017136871755 2307 36-37 0.1250187549229551 2307 38-39 -0.00445135012987663 2307 40-41 0.0756491097575136 2307 42-43 0.015310018323170027 2307 44-45 0.047847120361574014 2307 46-47 0.01242041507536129 2307 48-49 0.00210389121158272 2307 50-51 -0.023213107879250572 2307 52-53 -0.04500325630186097 2307 54-55 0.03350357688482575 2307 56-57 -0.0904369982881903 2307 58-59 -0.13456658114864695 2307 60-61 -0.17238253047135998 2307 62-63 -0.15658077091158162 2307 64-65 -0.24017087709773932 2307 66-67 -0.25822759783962823 2307 68-69 -0.3752463590214248 2307 70-71 -0.3624892547927274 2307 72-73 -0.3477054940898796 2307 74-75 -0.43406522456388785 2307 76-77 -0.9155547300847466 2307 78-79 -0.2599443347585506 2307 80-81 -0.15548711446657393 2307 82-83 -0.1682898833162625 2307 84-85 -0.22430982692903711 2307 86-87 -0.24807726455090773 2307 88-89 -0.26478878432575925 2307 90-91 -0.41360088437252784 2307 92-93 -0.3744753988309668 2307 94-95 -0.2800241578894358 2307 96-97 -0.1809894798488898 2307 98-99 -0.22829251609689294 2307 100-101 -0.720724112452146 2201 1 0.10111025118715844 2201 2 0.07665239898064158 2201 3 0.046911662589728564 2201 4 -0.11856249654188389 2201 5 -0.03035480536179591 2201 6 0.04189795933575624 2201 7 0.08968477637870365 2201 8 0.11092446334832573 2201 9 0.09157423340503357 2201 10-11 0.06342952739682062 2201 12-13 0.059553193450781805 2201 14-15 0.17006040863719818 2201 16-17 0.13144751150247203 2201 18-19 0.1597492249464878 2201 20-21 0.12177447177423772 2201 22-23 0.10341195600056352 2201 24-25 0.12017243948953649 2201 26-27 0.1679025952816815 2201 28-29 0.1461657011482771 2201 30-31 0.14476164087509602 2201 32-33 0.1694667688397118 2201 34-35 0.11783665901227636 2201 36-37 0.08506406171172642 2201 38-39 0.1324202999811277 2201 40-41 0.20918851513019376 2201 42-43 0.12075165547014421 2201 44-45 0.09290921934074703 2201 46-47 0.028169063137490014 2201 48-49 0.03786057873611526 2201 50-51 0.06955593711358432 2201 52-53 0.020361157824538623 2201 54-55 0.06226207116807103 2201 56-57 0.05031462931586361 2201 58-59 0.003982813640675431 2201 60-61 -0.08537279085793159 2201 62-63 0.08608306432322621 2201 64-65 -0.01922769786215639 2201 66-67 -0.08626459179272672 2201 68-69 -0.14963568113211778 2201 70-71 -0.17453274816596576 2201 72-73 -0.08647117625606882 2201 74-75 -0.2527080001941968 2201 76-77 -0.9030742142179271 2201 78-79 -0.07130548960878258 2201 80-81 -0.0030342130485507823 2201 82-83 0.01526757189211736 2201 84-85 -0.13984947559604421 2201 86-87 -0.007146460091355777 2201 88-89 0.004739818857586897 2201 90-91 -0.005962892133588582 2201 92-93 -0.14137546171569682 2201 94-95 -0.12333600219606211 2201 96-97 0.019412394651574516 2201 98-99 -0.10601573975366563 2201 100-101 -0.49744468141965115 1101 1 0.10550358134156568 1101 2 0.0777545584618835 1101 3 0.062094562624054106 1101 4 -0.1879802712354035 1101 5 -0.07993023367148311 1101 6 0.02782119624868784 1101 7 0.07058378952012134 1101 8 0.1537788042552961 1101 9 0.07755632039422267 1101 10-11 0.059143689173680514 1101 12-13 0.05579402737579642 1101 14-15 0.07727564145625365 1101 16-17 0.09136422372906594 1101 18-19 0.12024688439931452 1101 20-21 0.07545511938598537 1101 22-23 0.0615836599241959 1101 24-25 0.09254414864955862 1101 26-27 0.13602429078056133 1101 28-29 0.0653988292802481 1101 30-31 0.11169333510320456 1101 32-33 0.08683249948516192 1101 34-35 0.1495774125213174 1101 36-37 0.05911886845659353 1101 38-39 0.09923835818768367 1101 40-41 0.12716076372948493 1101 42-43 0.13889234428150132 1101 44-45 0.09120915366694504 1101 46-47 0.13579924783513775 1101 48-49 0.10584548042108821 1101 50-51 0.07706303474659393 1101 52-53 0.007071983182491692 1101 54-55 0.06490763798571209 1101 56-57 -0.00855978611750885 1101 58-59 -0.027346445465965985 1101 60-61 0.014822994432975634 1101 62-63 -0.020455812259747574 1101 64-65 -0.09142445325338144 1101 66-67 -0.10311654056405928 1101 68-69 -0.12583745287428627 1101 70-71 -0.1909865717469259 1101 72-73 0.0034492452312591126 1101 74-75 -0.14655874195055674 1101 76-77 -0.6264982778690609 1101 78-79 0.012122288776659218 1101 80-81 0.04054837360523322 1101 82-83 0.05363820020311749 1101 84-85 -0.053598699830523344 1101 86-87 0.0015385513297161424 1101 88-89 0.03327463897257843 1101 90-91 0.0012396602318958116 1101 92-93 -0.10431743824079831 1101 94-95 -0.14879812490580946 1101 96-97 0.09820005753724104 1101 98-99 -0.05201698608716043 1101 100-101 -0.3401830281056242 2304 1 0.06434860101606432 2304 2 0.06262260345552306 2304 3 0.06697922710149129 2304 4 -0.20318225025045678 2304 5 -0.06669482231907153 2304 6 0.04605904474197331 2304 7 0.0552322110602077 2304 8 0.15087315992320782 2304 9 0.09184626532489659 2304 10-11 0.04402446657640979 2304 12-13 0.05211986212601971 2304 14-15 0.05802233032159165 2304 16-17 0.07060282193306477 2304 18-19 0.06209580901292355 2304 20-21 0.04631171226897379 2304 22-23 0.05926209047163411 2304 24-25 0.04384279415025816 2304 26-27 0.07160818302933336 2304 28-29 0.04718979623910968 2304 30-31 0.006270438356793306 2304 32-33 0.0787018468490217 2304 34-35 0.12618927486152387 2304 36-37 0.0681113932176558 2304 38-39 0.0048974373055514775 2304 40-41 0.08229752651387656 2304 42-43 0.1234861929009412 2304 44-45 0.06943809134004653 2304 46-47 0.019808544851940724 2304 48-49 -0.010011052998748937 2304 50-51 -0.06901676422351244 2304 52-53 -0.04414562532702604 2304 54-55 0.04316954335230605 2304 56-57 -0.031854301126308826 2304 58-59 -0.08871445180430726 2304 60-61 -0.06646604501219144 2304 62-63 -0.02022135694923577 2304 64-65 -0.013711265519425808 2304 66-67 -0.07929673945734805 2304 68-69 -0.22013388666815814 2304 70-71 -0.13160243336991329 2304 72-73 -0.06830769014420923 2304 74-75 -0.18668855976140009 2304 76-77 -0.3749316783524925 2304 78-79 -0.02834837333524831 2304 80-81 0.10928928034515195 2304 82-83 0.05609997884279494 2304 84-85 0.027258408965273873 2304 86-87 0.11409463818554855 2304 88-89 -0.002133032832368542 2304 90-91 -0.014100076493782865 2304 92-93 -0.0885530107799184 2304 94-95 -0.028144102870108156 2304 96-97 0.05098175301198182 2304 98-99 -0.02053739820869538 2304 100-101 -0.3216646509640668 2202 1 0.09258482452214878 2202 2 0.06331896055322517 2202 3 0.03403418838693284 2202 4 -0.19040485169342247 2202 5 -0.06752305555892946 2202 6 0.01858071895711788 2202 7 0.046934818585839366 2202 8 0.1481897475871321 2202 9 0.026439245145056134 2202 10-11 0.06451835715051857 2202 12-13 0.06464394642613058 2202 14-15 0.06069321229637126 2202 16-17 0.09528509216217884 2202 18-19 0.07690959224012772 2202 20-21 0.08010412257712574 2202 22-23 0.06338941416803578 2202 24-25 0.0742231043282473 2202 26-27 0.11842056077556151 2202 28-29 0.0809834714044868 2202 30-31 0.11082234270691771 2202 32-33 0.07246910328433387 2202 34-35 0.06365525374848602 2202 36-37 0.07410747844907917 2202 38-39 0.07536828383596372 2202 40-41 0.14218124617952554 2202 42-43 0.13171451471391293 2202 44-45 0.025187014012679754 2202 46-47 0.142874483585004 2202 48-49 0.13656648922714254 2202 50-51 0.03187674747029945 2202 52-53 0.077917553511206 2202 54-55 0.05601949979393339 2202 56-57 0.10588484436546963 2202 58-59 0.07418757419907251 2202 60-61 0.03710188316397023 2202 62-63 0.07738927101005544 2202 64-65 0.09296410951191802 2202 66-67 0.10865006249183295 2202 68-69 0.028759268690542683 2202 70-71 -0.06028724144638886 2202 72-73 -0.0011256260750016622 2202 74-75 -0.06643183346346149 2202 76-77 -0.041198862915539536 2202 78-79 0.1423048632428916 2202 80-81 0.16528791420478228 2202 82-83 0.0845641839980047 2202 84-85 0.10874671752348064 2202 86-87 0.08609582956285067 2202 88-89 0.10009340195547622 2202 90-91 0.10360083437364409 2202 92-93 0.12503242349309573 2202 94-95 0.06831515038088298 2202 96-97 0.04798852158216249 2202 98-99 0.0874800258024635 2202 100-101 0.06913551994060185 1102 1 0.09452993310951996 1102 2 0.0773003606446423 1102 3 0.028006183042535326 1102 4 -0.25142172287704767 1102 5 -0.08767823059965707 1102 6 0.012161172058860359 1102 7 0.06571852872261275 1102 8 0.14365243056827381 1102 9 0.03237498345711742 1102 10-11 0.07390247690720742 1102 12-13 0.042613822661138556 1102 14-15 0.01480344634815367 1102 16-17 0.031687805967365534 1102 18-19 0.013238703382491224 1102 20-21 0.020491049314344423 1102 22-23 0.021469459993895157 1102 24-25 0.05389804543439425 1102 26-27 0.08877036387671922 1102 28-29 0.07428744571387114 1102 30-31 0.02648571893607965 1102 32-33 0.05298854118500884 1102 34-35 0.06061914390705425 1102 36-37 0.10242498955393842 1102 38-39 0.022232786959385464 1102 40-41 0.07472535796649993 1102 42-43 0.06862720238311937 1102 44-45 -0.01893674218435848 1102 46-47 -0.055840590310666016 1102 48-49 -0.05883120541556508 1102 50-51 -0.0065222627614147655 1102 52-53 -0.05727478911622086 1102 54-55 -0.05246256248704384 1102 56-57 -0.042701205117090524 1102 58-59 -0.14817011364593213 1102 60-61 -0.09334348326712671 1102 62-63 0.020309746731726364 1102 64-65 -0.02842035012967159 1102 66-67 -0.0605589872368526 1102 68-69 -0.18716467620122046 1102 70-71 -0.20492548377260533 1102 72-73 -0.13298343405175217 1102 74-75 -0.22479495067687338 1102 76-77 -0.20768495107465412 1102 78-79 -0.05981962253468254 1102 80-81 -0.06775537424030631 1102 82-83 -0.1056613120581602 1102 84-85 -0.08873064789967344 1102 86-87 -0.1093587665015221 1102 88-89 -0.15364722190913938 1102 90-91 -0.17254980789162033 1102 92-93 -0.22598446234074032 1102 94-95 -0.2791016803681501 1102 96-97 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1213 44-45 -0.3408619260802581 1213 46-47 -0.2922117438176457 1213 48-49 -0.28369508948943434 1213 50-51 -0.2654358472751426 1213 52-53 -0.28366118340257884 1213 54-55 -0.16326571860214756 1213 56-57 -0.14942786694992805 1213 58-59 -0.21465727878971563 1213 60-61 -0.24371102566250613 1213 62-63 -0.13816333382973056 1213 64-65 -0.15230226838351513 1213 66-67 -0.04214654021437525 1213 68-69 -0.011763107051194766 1213 70-71 -0.1321919595806591 1213 72-73 -0.06450942710743846 1213 74-75 0.015031067498050277 1213 76-77 0.5173715760941953 1213 78-79 -0.011909052328228142 1213 80-81 -0.1799124745194689 1213 82-83 -0.24798180311233153 1213 84-85 -0.1847961841417387 1213 86-87 -0.1660496824269302 1213 88-89 -0.07251344509970181 1213 90-91 -0.1267395613847171 1213 92-93 0.07331397189409472 1213 94-95 -0.018856260173357953 1213 96-97 -0.03054688028040431 1213 98-99 0.0773823742474633 1213 100-101 0.3410292740671217 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 27.0 8 403.0 9 1170.0 10 758.0 11 241.0 12 198.0 13 337.0 14 704.0 15 1528.0 16 2920.0 17 4620.0 18 6978.0 19 9838.0 20 13216.0 21 17592.0 22 23392.0 23 30818.0 24 39937.0 25 51977.0 26 67456.0 27 86813.0 28 111036.0 29 142570.0 30 185048.0 31 243811.0 32 332130.0 33 482495.0 34 775743.0 35 1479661.0 36 3217144.0 37 4492045.0 38 895527.0 39 94.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 20.060120473979154 23.357417422325426 30.55801051820942 26.024451585486 2 26.226112141871326 23.8331610478157 30.350758741645556 19.58996806866742 3 20.9654976333576 21.715782912856813 36.62866897584561 20.690050477939973 4 20.918619577472235 22.187804495107173 35.5341401393488 21.3594357880718 5 27.78201273148958 21.492481382431468 30.989063021274426 19.736442864804527 6 28.06561353785971 21.99362033817759 30.66393127533716 19.276834848625544 7 27.690710711373416 26.812176443748577 25.649836938775046 19.847275906102965 8 25.410817811190533 23.337502414840934 30.700335714980444 20.551344058988086 9 22.22554728335211 22.288417671988764 34.14231651277863 21.3437185318805 10-11 26.560163601439523 23.249371541650994 28.5906945115889 21.59977034532058 12-13 25.369810996302157 26.624661913513787 25.626661782283332 22.378865307900732 14-15 25.22844278194876 24.727398159264084 26.948116241188778 23.096042817598377 16-17 24.807526268004768 24.611818988319836 26.550697428077445 24.02995731559795 18-19 24.65959293546295 25.086991278424065 26.95618234268309 23.2972334434299 20-21 23.848881453121802 25.516059464190743 27.32067930229874 23.31437978038872 22-23 24.000384961013655 25.62743101975905 26.722403716697947 23.64978030252935 24-25 24.423703174396373 25.575156729761666 26.45338828228364 23.547751813558317 26-27 24.241967965822912 25.56314640977136 26.62704453583907 23.567841088566656 28-29 24.159118380397146 26.03870790500784 26.15459668166063 23.64757703293438 30-31 24.19609286796141 25.991944318662608 26.123527104284722 23.68843570909126 32-33 24.032866525677434 26.31888056112215 25.991393927566484 23.656858985633928 34-35 23.87734565873912 26.288821344544118 26.202728383016222 23.63110461370054 36-37 23.99443715466138 26.15257550901277 26.187258766191285 23.66572857013456 38-39 24.060881042913852 25.937263216099783 26.30572609325948 23.69612964772689 40-41 23.93244309251612 26.142895178759527 26.003404179563788 23.92125754916056 42-43 23.841989137160084 25.969196346889067 26.14725055446843 24.04156396148242 44-45 24.141737815854707 25.928024255578354 26.0422736530377 23.887964275529242 46-47 23.838830076105722 25.898291342293017 26.211927777051347 24.050950804549913 48-49 23.951906655531467 25.59900750305143 26.123640440248526 24.325445401168583 50-51 23.979406618946104 25.858453961596524 26.389462329456197 23.772677090001178 52-53 23.978536977706884 25.824854824324184 26.48122990136877 23.71537829660016 54-55 23.87071570557312 25.876808466485844 26.030617613710845 24.221858214230192 56-57 24.00687105391611 26.075661406761398 26.17411532133756 23.743352217984935 58-59 23.807788921162068 26.369115488176153 26.18535587324408 23.63773971741769 60-61 24.235269668483284 26.129219561254207 25.840574426581252 23.794936343681254 62-63 23.852774338263487 26.349966551881067 26.210300605337046 23.586958504518403 64-65 24.096691867865943 26.17999161960722 26.028196271031874 23.695120241494962 66-67 24.246129442282992 26.09956363139954 26.189802726666798 23.464504199650666 68-69 24.01549674567712 25.889730665282972 26.54717765483966 23.54759493420025 70-71 24.0133601400206 26.26899761159794 26.210617683314098 23.507024565067372 72-73 23.810560012683396 25.95954949721359 26.507429278937757 23.722461211165257 74-75 23.89474838418036 25.961018406408705 26.500959700368888 23.64327350904205 76-77 23.827996213863923 26.007340366799724 26.256833255684224 23.90783016365213 78-79 23.946066596884954 26.032558936655974 26.04297390247039 23.978400563988682 80-81 23.849294691083532 25.888252987132425 26.38681214334713 23.875640178436914 82-83 23.571752920617012 26.481739140690884 26.315778506853597 23.63072943183851 84-85 23.770093210130995 26.13579156109158 26.140859636869422 23.953255591908 86-87 23.711163268092193 26.31120170690999 26.272935895250132 23.70469912974768 88-89 23.794572420570013 26.981908225841355 25.71242661565097 23.511092737937663 90-91 23.762159751603164 26.30476668751584 25.857456535763873 24.075617025117126 92-93 23.739596714514175 26.49764472868288 25.822988453050534 23.939770103752412 94-95 23.63322482155676 26.705936571784623 25.684097792003634 23.976740814654985 96-97 23.620143774466822 26.26570703790967 25.812841411085024 24.301307776538483 98-99 23.78218367412416 26.28117790973379 25.765831785237296 24.170806630904757 100-101 23.93453178892506 26.890478075856084 25.2673864528554 23.907603682363458 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1282.0 1 1189.0 2 1336.5 3 1568.5 4 1724.0 5 1830.5 6 1846.5 7 1973.5 8 2104.0 9 2305.0 10 2570.0 11 2882.5 12 3168.0 13 3467.5 14 3837.0 15 4263.5 16 4748.5 17 5330.5 18 5989.5 19 6649.5 20 7647.0 21 8844.5 22 9967.0 23 11262.0 24 13012.0 25 15364.5 26 18387.0 27 22006.5 28 26336.0 29 31896.0 30 38828.5 31 47720.0 32 59714.0 33 75543.0 34 95421.0 35 120977.5 36 151104.5 37 184413.0 38 219354.0 39 261461.5 40 311683.5 41 361016.0 42 406631.5 43 451077.0 44 498542.5 45 561930.5 46 646150.0 47 733514.0 48 783256.5 49 781291.0 50 756845.5 51 728771.5 52 683246.0 53 633100.5 54 593985.5 55 545234.0 56 479314.5 57 369808.0 58 252450.0 59 172561.0 60 118650.5 61 90381.0 62 74223.5 63 56125.5 64 40178.5 65 29395.0 66 20245.5 67 14661.5 68 11785.0 69 9198.0 70 7140.0 71 5323.5 72 3911.0 73 2703.0 74 1749.5 75 1158.5 76 781.5 77 514.5 78 351.0 79 235.5 80 150.5 81 95.0 82 60.0 83 39.0 84 22.5 85 14.5 86 11.0 87 8.5 88 6.5 89 6.5 90 4.5 91 1.5 92 1.0 93 1.0 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12633048201915534 2 0.0 3 0.0 4 0.34249265365484455 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.7519554805940358E-5 12-13 0.001136164476988109 14-15 3.931364972277194E-6 16-17 0.0 18-19 0.0 20-21 9.513903232910808E-4 22-23 4.5603833678415447E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0021779761946415653 38-39 0.00736737795804746 40-41 0.0072415742789345905 42-43 0.0015096441493544423 44-45 0.0 46-47 0.0 48-49 2.7519554805940358E-5 50-51 0.0012737622510178106 52-53 0.012320897823116725 54-55 0.009199394035128632 56-57 0.0087394243333722 58-59 0.012702240225427612 60-61 0.0028581023348455198 62-63 1.965682486138597E-4 64-65 0.0035225030151603658 66-67 0.011455997529215741 68-69 0.019869118569888936 70-71 0.0254949018452176 72-73 0.018807650027374095 74-75 0.003597198949633632 76-77 0.014424178083285024 78-79 0.007489250272188054 80-81 0.005354519092241538 82-83 0.003569679394827692 84-85 0.010779802753984064 86-87 0.01507285330371076 88-89 0.01274548524012266 90-91 0.030637127228956174 92-93 0.013787296957776118 94-95 0.01407821796572463 96-97 0.006923133716180138 98-99 0.012053565005001876 100-101 0.13952021150114532 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2718229E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.58847832032106 #Duplication Level Percentage of deduplicated Percentage of total 1 84.45142044716786 31.744003865986876 2 7.895277689115562 5.935429485004697 3 2.3693699526385688 2.671830332927249 4 1.1060731605850445 1.6630242806935973 5 0.7460569719727739 1.402157315836149 6 0.4058295155755733 0.9152708368775301 7 0.3570075122536226 0.9393558394175933 8 0.23337506976493916 0.7017771000290264 9 0.18641030326230684 0.6306191678573724 >10 1.622473371943423 12.852568551243504 >50 0.31246559955335385 8.152482184500641 >100 0.282928003138643 21.736187456069274 >500 0.02277894994402347 5.795887854429664 >1k 0.008512258840065644 4.804977959994019 >5k 2.119424408390097E-5 0.05442776913286895 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.0221548927920703E-4 0.0 0.0 2 1.0221548927920703E-4 0.0 0.0 3 1.1007821922376142E-4 0.0 0.0 4 1.179409491683158E-4 0.0 0.0 5 1.258036791128702E-4 0.0 0.0 6 1.3366640905742457E-4 0.0 0.0 7 1.3366640905742457E-4 0.0 0.0 8 1.4152913900197897E-4 0.0 0.0 9 1.6511732883564215E-4 7.862729944554388E-6 0.0 10-11 2.358818983366316E-4 1.1794094916831582E-5 0.0 12-13 2.7126418308712636E-4 2.7519554805940358E-5 0.0 14-15 3.2237192772672986E-4 3.9313649722771936E-5 0.0 16-17 4.245874170059369E-4 3.9313649722771936E-5 0.0 18-19 5.464597311465299E-4 3.9313649722771936E-5 0.0 20-21 6.761947752316773E-4 4.717637966732632E-5 0.0 22-23 8.452434690395966E-4 4.717637966732632E-5 0.0 24-25 0.0010024980679306844 5.503910961188071E-5 0.0 26-27 0.0012265858713504845 5.503910961188071E-5 0.0 28-29 0.0015135755143267195 5.89704745841579E-5 0.0 30-31 0.001871329726803944 6.29018395564351E-5 3.931364972277194E-6 32-33 0.0022448093991702775 6.29018395564351E-5 7.862729944554388E-6 34-35 0.002704779100926709 7.076456950098949E-5 7.862729944554388E-6 36-37 0.0033573856863247232 7.862729944554387E-5 7.862729944554388E-6 38-39 0.004013923636695014 7.862729944554387E-5 7.862729944554388E-6 40-41 0.004792333901205899 7.862729944554387E-5 7.862729944554388E-6 42-43 0.005759449684386088 7.862729944554387E-5 7.862729944554388E-6 44-45 0.006946721906013802 7.862729944554387E-5 7.862729944554388E-6 46-47 0.008566444274592005 7.862729944554387E-5 7.862729944554388E-6 48-49 0.010512469935869215 9.435275933465264E-5 1.9656824861385968E-5 50-51 0.012894877109069194 1.0614685425148422E-4 2.358818983366316E-5 52-53 0.015894508582916696 1.179409491683158E-4 2.358818983366316E-5 54-55 0.01974331489077607 1.21872314140593E-4 2.358818983366316E-5 56-57 0.024771530690318597 1.258036791128702E-4 2.358818983366316E-5 58-59 0.030951636426738344 1.258036791128702E-4 2.358818983366316E-5 60-61 0.03867283723229076 1.3759777402970176E-4 2.358818983366316E-5 62-63 0.04854842604265107 1.4152913900197897E-4 2.358818983366316E-5 64-65 0.060550883303013336 1.4152913900197897E-4 2.358818983366316E-5 66-67 0.07577705984064291 1.4152913900197897E-4 2.358818983366316E-5 68-69 0.09479307221154769 1.4152913900197897E-4 2.358818983366316E-5 70-71 0.11815717424179106 1.4152913900197897E-4 2.358818983366316E-5 72-73 0.14750088239486803 1.4152913900197897E-4 2.358818983366316E-5 74-75 0.18466800684277662 1.5332323391881056E-4 2.358818983366316E-5 76-77 0.23091265301167324 1.6118596386336493E-4 2.358818983366316E-5 78-79 0.2887587572137599 1.6511732883564215E-4 2.358818983366316E-5 80-81 0.35868987734062663 1.6904869380791933E-4 2.358818983366316E-5 82-83 0.44538826907425555 1.847741536970281E-4 2.358818983366316E-5 84-85 0.5496873817887695 1.887055186693053E-4 2.358818983366316E-5 86-87 0.674885630695909 1.887055186693053E-4 2.358818983366316E-5 88-89 0.8241988723429967 1.887055186693053E-4 2.358818983366316E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 103520 0.0 68.27768 1 TTTGTGT 117155 0.0 60.337788 2 TTGTGTT 128175 0.0 56.495758 3 TGTGTTT 138705 0.0 52.457542 4 ACTTTGT 27960 0.0 51.39798 1 GTGTTTG 145925 0.0 49.87719 5 AACTTTG 12620 0.0 47.417038 2 TAACTTT 12880 0.0 46.733425 1 TTTGAGG 40700 0.0 35.87406 8 GTTTGAG 111550 0.0 34.367584 7 TGTTTGA 212785 0.0 34.259583 6 TTGAGGG 18605 0.0 34.019688 9 TTTGAGC 29605 0.0 29.976027 8 TTGAGGC 8490 0.0 28.802444 9 GTTTGAC 42355 0.0 28.216839 7 TTGAGTC 13420 0.0 28.188509 4 TTTGAGT 47650 0.0 27.326302 3 GTTTGAA 21650 0.0 26.385506 2 TTGAGCC 16665 0.0 24.759638 9 GTTTGAT 75360 0.0 24.383718 7 >>END_MODULE