FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l04n01_6777.34100000035b47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l04n01_6777.34100000035b47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12718229
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1035200.068.277681
TTTGTGT1171550.060.3377882
TTGTGTT1281750.056.4957583
TGTGTTT1387050.052.4575424
ACTTTGT279600.051.397981
GTGTTTG1459250.049.877195
AACTTTG126200.047.4170382
TAACTTT128800.046.7334251
TTTGAGG407000.035.874068
GTTTGAG1115500.034.3675847
TGTTTGA2127850.034.2595836
TTGAGGG186050.034.0196889
TTTGAGC296050.029.9760278
TTGAGGC84900.028.8024449
GTTTGAC423550.028.2168397
TTGAGTC134200.028.1885094
TTTGAGT476500.027.3263023
GTTTGAA216500.026.3855062
TTGAGCC166650.024.7596389
GTTTGAT753600.024.3837187
Produced by FastQC (version 0.11.2)