##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l04n01_30082.34100000035d38.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15063215 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.730189936212156 33.0 33.0 33.0 33.0 33.0 2 32.765995771818964 33.0 33.0 33.0 33.0 33.0 3 32.79261007693245 33.0 33.0 33.0 33.0 33.0 4 36.6081564261016 37.0 37.0 37.0 37.0 37.0 5 36.63213430864526 37.0 37.0 37.0 37.0 37.0 6 36.6350661528764 37.0 37.0 37.0 37.0 37.0 7 36.61058552241337 37.0 37.0 37.0 37.0 37.0 8 36.56735517616923 37.0 37.0 37.0 37.0 37.0 9 36.5903437612754 37.0 37.0 37.0 37.0 37.0 10-11 36.585450682341055 37.0 37.0 37.0 37.0 37.0 12-13 36.55880779767135 37.0 37.0 37.0 37.0 37.0 14-15 38.62486398156038 40.0 37.0 40.0 37.0 40.0 16-17 38.60927776706367 40.0 37.0 40.0 37.0 40.0 18-19 38.57238069694949 40.0 37.0 40.0 37.0 40.0 20-21 38.51144596289703 40.0 37.0 40.0 37.0 40.0 22-23 38.46405893429789 40.0 37.0 40.0 37.0 40.0 24-25 38.423204375692706 40.0 37.0 40.0 37.0 40.0 26-27 38.32504720937728 40.0 37.0 40.0 37.0 40.0 28-29 38.27831764334506 40.0 37.0 40.0 37.0 40.0 30-31 38.22243179825821 40.0 37.0 40.0 37.0 40.0 32-33 38.121740843505194 40.0 37.0 40.0 37.0 40.0 34-35 38.04141446563699 40.0 37.0 40.0 37.0 40.0 36-37 37.95942934493068 40.0 37.0 40.0 35.0 40.0 38-39 37.8386144989632 40.0 37.0 40.0 33.0 40.0 40-41 37.682835038867864 40.0 37.0 40.0 33.0 40.0 42-43 37.577662471125855 40.0 37.0 40.0 33.0 40.0 44-45 37.46036466982646 40.0 37.0 40.0 33.0 40.0 46-47 37.30973646728138 40.0 37.0 40.0 33.0 40.0 48-49 37.12008415866069 37.0 37.0 40.0 33.0 40.0 50-51 36.92607876207038 37.0 37.0 40.0 33.0 40.0 52-53 36.68705551902433 37.0 37.0 40.0 33.0 40.0 54-55 36.42952938665484 37.0 37.0 40.0 33.0 40.0 56-57 36.15043820326537 37.0 37.0 40.0 33.0 40.0 58-59 35.89935617993901 37.0 37.0 40.0 33.0 40.0 60-61 35.606197415359205 37.0 33.0 37.0 33.0 40.0 62-63 35.28923951493755 37.0 33.0 37.0 33.0 40.0 64-65 35.055438463833916 37.0 33.0 37.0 30.0 40.0 66-67 34.78564718753599 37.0 33.0 37.0 27.0 40.0 68-69 34.53139353053116 37.0 33.0 37.0 27.0 37.0 70-71 34.2710745016917 37.0 33.0 37.0 27.0 37.0 72-73 34.05388723456447 37.0 33.0 37.0 27.0 37.0 74-75 33.81954602652887 37.0 33.0 37.0 27.0 37.0 76-77 31.025076651963076 33.0 30.0 35.0 24.5 37.0 78-79 33.55895849591206 35.0 33.0 37.0 27.0 37.0 80-81 34.01304562140287 37.0 33.0 37.0 27.0 37.0 82-83 33.998021504705335 37.0 33.0 37.0 27.0 37.0 84-85 33.947632660092815 37.0 33.0 37.0 27.0 37.0 86-87 33.84212716209654 37.0 33.0 37.0 27.0 37.0 88-89 33.65458542548852 37.0 33.0 37.0 27.0 37.0 90-91 33.524346927266194 37.0 33.0 37.0 27.0 37.0 92-93 33.42561119920283 37.0 33.0 37.0 27.0 37.0 94-95 33.320443710057916 37.0 33.0 37.0 27.0 37.0 96-97 33.21017946699958 37.0 33.0 37.0 27.0 37.0 98-99 33.07338997684093 33.0 33.0 37.0 27.0 37.0 100-101 31.188515499513215 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1196479896566558 2306 2 0.1096357770249341 2306 3 0.055007309924341996 2306 4 -0.16823247196300173 2306 5 -0.05143380134879294 2306 6 0.06614218528282834 2306 7 0.10428028267966738 2306 8 0.15981885403544993 2306 9 0.12082457755077058 2306 10-11 0.09286707352303836 2306 12-13 0.09573066068900715 2306 14-15 0.1961225054980531 2306 16-17 0.2089978828172505 2306 18-19 0.17623717438272735 2306 20-21 0.15317390369823158 2306 22-23 0.18849210971501407 2306 24-25 0.16129057366395472 2306 26-27 0.1886829962273069 2306 28-29 0.13341658696317182 2306 30-31 0.14850312819545053 2306 32-33 0.19150590288190728 2306 34-35 0.1905805503184297 2306 36-37 0.10900650005208234 2306 38-39 0.12041430895005334 2306 40-41 0.255746684001636 2306 42-43 0.18064832619411675 2306 44-45 0.13156062251791667 2306 46-47 0.12113764892286127 2306 48-49 0.12857836446092819 2306 50-51 0.06413402620991349 2306 52-53 0.05985004979484643 2306 54-55 0.03279100774064858 2306 56-57 0.08127803842594972 2306 58-59 -0.0029740091301846405 2306 60-61 -0.04194745807979672 2306 62-63 0.03622491826142493 2306 64-65 0.017780073023963894 2306 66-67 -0.024082988614694045 2306 68-69 -0.11915946304036851 2306 70-71 -0.14901634304957412 2306 72-73 -0.1327553810464721 2306 74-75 -0.24306899458315456 2306 76-77 -0.8392836499424519 2306 78-79 -0.07128297595331645 2306 80-81 0.020362416851966714 2306 82-83 -0.06777713258667717 2306 84-85 -0.13038734587709655 2306 86-87 -0.11785008100989813 2306 88-89 -0.15140595414516156 2306 90-91 -0.19763890222151304 2306 92-93 -0.2617844656858139 2306 94-95 -0.2822682143982078 2306 96-97 -0.20271888026645968 2306 98-99 -0.01355648166594392 2306 100-101 -0.3886605855831604 2307 1 0.11882231949210365 2307 2 0.09199309807453204 2307 3 0.07865082597591311 2307 4 -0.15887033367365433 2307 5 -0.03756247419367753 2307 6 0.06327056092162309 2307 7 0.08873949168158646 2307 8 0.1307724791387912 2307 9 0.09673451961219826 2307 10-11 0.07087565498242299 2307 12-13 0.07113072626584227 2307 14-15 0.13699578725704242 2307 16-17 0.11158802604869322 2307 18-19 0.10472154448853388 2307 20-21 0.13680050577288227 2307 22-23 0.11405942454887708 2307 24-25 0.14308953416563241 2307 26-27 0.13135336360803507 2307 28-29 0.12916491320049062 2307 30-31 0.13285027428425877 2307 32-33 0.14224572816537062 2307 34-35 0.16594656713743916 2307 36-37 0.09143460539160486 2307 38-39 0.05369088295524449 2307 40-41 0.14373938711347023 2307 42-43 -0.010460732221439173 2307 44-45 0.039173470457626536 2307 46-47 0.006450978586549638 2307 48-49 0.06424423799182222 2307 50-51 -0.04976830143547062 2307 52-53 -0.012449556411972651 2307 54-55 0.015853997844907042 2307 56-57 -0.1010444864540716 2307 58-59 -0.09115279334430682 2307 60-61 -0.13302937518997737 2307 62-63 -0.12216037728667573 2307 64-65 -0.266844362826717 2307 66-67 -0.22051112736792078 2307 68-69 -0.3380889700944678 2307 70-71 -0.32552940243353845 2307 72-73 -0.33758042221274565 2307 74-75 -0.3767294207035974 2307 76-77 -0.9144672152469937 2307 78-79 -0.2828606625175567 2307 80-81 -0.08974491538437945 2307 82-83 -0.0942199499206069 2307 84-85 -0.17409300555247853 2307 86-87 -0.1598305629136476 2307 88-89 -0.24090467729231335 2307 90-91 -0.3076130681539624 2307 92-93 -0.31064956245560893 2307 94-95 -0.250277623384072 2307 96-97 -0.17276327652483303 2307 98-99 -0.2474041075637885 2307 100-101 -0.6859267096972772 2201 1 0.12652007044916047 2201 2 0.0993990643474163 2201 3 0.07868858316635396 2201 4 -0.10242429515687235 2201 5 -2.1178180429615168E-4 2201 6 0.05689178847666909 2201 7 0.10339784227449655 2201 8 0.15233330672354128 2201 9 0.11504928013248161 2201 10-11 0.1038933117705767 2201 12-13 0.10826137864039964 2201 14-15 0.2032562322115652 2201 16-17 0.18023049314977868 2201 18-19 0.15731661991760149 2201 20-21 0.16349664167137945 2201 22-23 0.1306142704574924 2201 24-25 0.15630582741759014 2201 26-27 0.18376701228269354 2201 28-29 0.19182737124705795 2201 30-31 0.1365991547850669 2201 32-33 0.18043189977008467 2201 34-35 0.17351781498585694 2201 36-37 0.10547078837455359 2201 38-39 0.12342201571421896 2201 40-41 0.17210678181807282 2201 42-43 0.12177777475411489 2201 44-45 0.046959441675895164 2201 46-47 0.01000034973258579 2201 48-49 0.01113102997991433 2201 50-51 0.05367887598475818 2201 52-53 0.04311247724016454 2201 54-55 0.07091580128933117 2201 56-57 0.010012101511833293 2201 58-59 -0.05016903019777885 2201 60-61 -0.07737117634673751 2201 62-63 -0.0014350349247820304 2201 64-65 -0.09053579532670852 2201 66-67 -0.1581950622571071 2201 68-69 -0.1869692546292967 2201 70-71 -0.17420930652486533 2201 72-73 -0.13617601392709133 2201 74-75 -0.22072545070172822 2201 76-77 -0.8470027604728401 2201 78-79 -0.1282935083055321 2201 80-81 0.0025224240271128906 2201 82-83 0.04366816816489205 2201 84-85 -0.021864994895643974 2201 86-87 0.0546717156141554 2201 88-89 0.03181037288599242 2201 90-91 0.015045057878282364 2201 92-93 -0.11819584610275768 2201 94-95 -0.08190185060822586 2201 96-97 0.051830940311134555 2201 98-99 -0.09665416926090842 2201 100-101 -0.48154715795833525 1101 1 0.09629982177396101 1101 2 0.07572638924816744 1101 3 0.04094728176523432 1101 4 -0.1966966644107231 1101 5 -0.049549615486732534 1101 6 0.04154929816706243 1101 7 0.049600926100680454 1101 8 0.14440212055911417 1101 9 0.07341115260129527 1101 10-11 0.07596805498041448 1101 12-13 0.10119680154956967 1101 14-15 0.12619298922994915 1101 16-17 0.12964661870358896 1101 18-19 0.12647391794312313 1101 20-21 0.09973047550825243 1101 22-23 0.10070705482269915 1101 24-25 0.14464594390911856 1101 26-27 0.15423217169323067 1101 28-29 0.09674699375275253 1101 30-31 0.08255398053730545 1101 32-33 0.10214668514742442 1101 34-35 0.14843760640977166 1101 36-37 0.13308755223238933 1101 38-39 0.09275096912450209 1101 40-41 0.149891974377951 1101 42-43 0.15132998289445965 1101 44-45 0.12392789740484744 1101 46-47 0.12498791528319231 1101 48-49 0.12800412275532125 1101 50-51 0.05753629590717679 1101 52-53 -0.016082686045145067 1101 54-55 0.010825009469662916 1101 56-57 0.05733120733462016 1101 58-59 -0.05565545951480999 1101 60-61 -0.07501405358248547 1101 62-63 -0.028132914690857547 1101 64-65 -0.04965056732847728 1101 66-67 -0.05863908275895113 1101 68-69 -0.13615728887625522 1101 70-71 -0.20203328822378097 1101 72-73 -0.04750947848804543 1101 74-75 -0.18181200853450719 1101 76-77 -0.6656461093006811 1101 78-79 0.06183162906979334 1101 80-81 0.008780864767153673 1101 82-83 0.08322173454562432 1101 84-85 0.05291986534349036 1101 86-87 0.14186327094417805 1101 88-89 0.12438603101207235 1101 90-91 0.12382524425368757 1101 92-93 0.02535296712608215 1101 94-95 -0.006437470289888836 1101 96-97 0.06346309020571539 1101 98-99 0.07814338052860137 1101 100-101 -0.22529435012383914 2304 1 0.07376859017579562 2304 2 0.039645774436891656 2304 3 0.052719430898775954 2304 4 -0.17914913914026442 2304 5 -0.06080676434840626 2304 6 0.026559457694681043 2304 7 0.06313775082056594 2304 8 0.13618850881714906 2304 9 0.0787545267763079 2304 10-11 0.04399858634211995 2304 12-13 0.052162142436287695 2304 14-15 0.04961968476604994 2304 16-17 0.10842436606134243 2304 18-19 0.09781847238688357 2304 20-21 0.057033438633382616 2304 22-23 0.05066223107929346 2304 24-25 0.051058974509835764 2304 26-27 0.09775688279287209 2304 28-29 0.08537071460340684 2304 30-31 0.057556449662079956 2304 32-33 0.1191111525523425 2304 34-35 0.12181988002323862 2304 36-37 0.08908478923631691 2304 38-39 0.004257578517886884 2304 40-41 0.08030604588577717 2304 42-43 0.11997272007195647 2304 44-45 0.061022239019131064 2304 46-47 0.10520691078551181 2304 48-49 0.05613827603686872 2304 50-51 -0.06150447502740519 2304 52-53 0.012768723668038717 2304 54-55 0.06619391042450928 2304 56-57 -0.06330272231453904 2304 58-59 -0.11113687666008332 2304 60-61 -0.0703360435583349 2304 62-63 0.019119344075967604 2304 64-65 0.08172451592574959 2304 66-67 -0.013313529015668735 2304 68-69 -0.11207247790820674 2304 70-71 -0.13496834171138516 2304 72-73 -0.013736332137433749 2304 74-75 -0.1280461619276636 2304 76-77 -0.32398369071921707 2304 78-79 0.04105864073678589 2304 80-81 0.05822393003448667 2304 82-83 0.012928177374739391 2304 84-85 -0.016594739964084226 2304 86-87 0.07331820810147605 2304 88-89 0.04234395559509352 2304 90-91 -0.049194673140256384 2304 92-93 -0.10468161656608288 2304 94-95 -0.06571593733730907 2304 96-97 0.056635339124746054 2304 98-99 0.05570567430645923 2304 100-101 -0.3110303318811738 2202 1 0.09425177561944054 2202 2 0.06984493795149405 2202 3 0.026395259903075896 2202 4 -0.19077348936340144 2202 5 -0.07095756665995623 2202 6 0.020684572678476343 2202 7 0.029603764162906998 2202 8 0.10997829560159289 2202 9 0.037610655668096626 2202 10-11 0.030347799203831016 2202 12-13 0.022265558901914062 2202 14-15 -0.002704012106178766 2202 16-17 0.014677068872302357 2202 18-19 0.03395347663352055 2202 20-21 0.06645698252533805 2202 22-23 -0.005241617399875054 2202 24-25 0.034784654956858674 2202 26-27 0.06784808384279728 2202 28-29 0.07510451516227334 2202 30-31 0.04581275069420343 2202 32-33 0.06572626525944969 2202 34-35 0.00556350865940658 2202 36-37 0.026403419481276558 2202 38-39 0.011657921832345153 2202 40-41 0.04610040190993914 2202 42-43 0.05367155064245566 2202 44-45 -0.017153331687900675 2202 46-47 0.049258064314742 2202 48-49 0.028821950627154536 2202 50-51 -0.014658091991179845 2202 52-53 0.008951712600328676 2202 54-55 0.01178952400973543 2202 56-57 0.03415282271848952 2202 58-59 0.0349978152096142 2202 60-61 -0.02459258621355076 2202 62-63 -0.033826230903031274 2202 64-65 -0.03852604719700281 2202 66-67 0.010189499492810228 2202 68-69 -0.05655577338733764 2202 70-71 -0.10386452215757203 2202 72-73 -0.03826337129280688 2202 74-75 -0.07055867729089016 2202 76-77 -0.037244231822676 2202 78-79 0.043297409542958576 2202 80-81 0.04983954367036603 2202 82-83 0.0022166883620613476 2202 84-85 0.033182044200714245 2202 86-87 -0.005142863869231462 2202 88-89 0.023013510231884027 2202 90-91 0.03040686486588129 2202 92-93 0.03297005685968202 2202 94-95 0.00839855143671997 2202 96-97 0.012470833456092123 2202 98-99 -0.01548952066084297 2202 100-101 -0.10119624273011141 1102 1 0.047778694549180045 1102 2 0.035188883668865856 1102 3 0.013168380762074605 1102 4 -0.280157839867627 1102 5 -0.10316800750228339 1102 6 -0.02142822891058671 1102 7 0.025472402424590257 1102 8 0.10686952037082165 1102 9 0.02761466707437421 1102 10-11 0.023856439939713425 1102 12-13 0.017268439858177942 1102 14-15 0.016722559639447354 1102 16-17 0.01649485920383853 1102 18-19 0.03538529380077904 1102 20-21 -0.01190071092487699 1102 22-23 0.043271657168439503 1102 24-25 0.03759996723942294 1102 26-27 0.09205983949306784 1102 28-29 0.07362922349884826 1102 30-31 0.040182872114051804 1102 32-33 0.006791128162809912 1102 34-35 0.02392308220721162 1102 36-37 -0.01079593187697725 1102 38-39 -0.013101658790738213 1102 40-41 0.08335122353832247 1102 42-43 0.024011934052659 1102 44-45 -0.01579080763755769 1102 46-47 -0.06483681224106164 1102 48-49 -0.07618747797658187 1102 50-51 -0.01871211636305503 1102 52-53 -0.08845704207249838 1102 54-55 -0.07656942032532754 1102 56-57 -0.041520051957931514 1102 58-59 -0.13913209807903115 1102 60-61 -0.12414502431435182 1102 62-63 -0.027307135540539207 1102 64-65 -0.060399797066217786 1102 66-67 -0.06613141529310695 1102 68-69 -0.17740774682945215 1102 70-71 -0.24375663366324574 1102 72-73 -0.14549133497207123 1102 74-75 -0.2341730167185503 1102 76-77 -0.25706988560067145 1102 78-79 -0.07343932028383904 1102 80-81 -0.09667207492980623 1102 82-83 -0.16018219108556053 1102 84-85 -0.1743671664620834 1102 86-87 -0.17804316231492834 1102 88-89 -0.18573571825267976 1102 90-91 -0.22856543036172638 1102 92-93 -0.2009090112522287 1102 94-95 -0.33486543800604096 1102 96-97 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46-47 -0.3594967402467759 1213 48-49 -0.2836110094347717 1213 50-51 -0.26793520316397235 1213 52-53 -0.27958106586459053 1213 54-55 -0.2059523616979888 1213 56-57 -0.1952785902639178 1213 58-59 -0.2665511538146603 1213 60-61 -0.28437408181786594 1213 62-63 -0.14027454123822736 1213 64-65 -0.11817388467324008 1213 66-67 -0.048191507990296145 1213 68-69 -0.009013065390888642 1213 70-71 -0.11303599021046296 1213 72-73 -0.07477995379601765 1213 74-75 0.012063570541855029 1213 76-77 0.4583709092938193 1213 78-79 -0.0016053505111912614 1213 80-81 -0.1811132028753164 1213 82-83 -0.2410831901609214 1213 84-85 -0.19838705546524693 1213 86-87 -0.19439167650221378 1213 88-89 -0.09060227966156731 1213 90-91 -0.1089475863980951 1213 92-93 0.05867188051824712 1213 94-95 0.0370303531424625 1213 96-97 0.07853178618084655 1213 98-99 0.0838761686498728 1213 100-101 0.2694229207826666 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 46.0 8 473.0 9 1317.0 10 895.0 11 310.0 12 281.0 13 470.0 14 1163.0 15 2611.0 16 4778.0 17 7520.0 18 10741.0 19 14591.0 20 18733.0 21 24114.0 22 30802.0 23 39162.0 24 50428.0 25 64279.0 26 82410.0 27 104975.0 28 134724.0 29 172971.0 30 225196.0 31 298044.0 32 405157.0 33 580268.0 34 916046.0 35 1728181.0 36 3811738.0 37 5346900.0 38 983861.0 39 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.153740925492574 20.062628766358873 33.807928036077676 27.975702272070873 2 29.720288796249672 21.686884240847654 30.4368688888793 18.155958074023374 3 19.724182387358873 19.29025111837015 41.71768775789232 19.267878736378655 4 19.515180090650016 20.14830199254573 40.304264624877455 20.0322532919268 5 29.304434677457635 19.33151057061856 33.29893385973711 18.065120892186695 6 31.834087211793765 20.207983488252673 30.758891777087427 17.199037522866135 7 28.95451601799483 29.477418997206108 23.326786479513174 18.24127850528589 8 26.68435656000396 21.483893046736704 32.10685102748649 19.724899365772846 9 22.34009804679811 20.54127223172477 36.281185656581286 20.83744406489584 10-11 28.403458771518782 21.717333878945254 28.258277228368893 21.620930121167074 12-13 26.98224795111216 26.19203946079977 24.513707383298865 22.312005204789205 14-15 26.257787597136467 23.964920503358677 26.645523548591722 23.131768350913134 16-17 25.659535497568083 23.769736407533184 26.05940365320418 24.51132444169455 18-19 25.434556965428694 24.472627523407187 26.892585679750308 23.200229831413814 20-21 24.190507253474976 25.089476160287294 27.516873114451514 23.203143471786216 22-23 24.32196461935722 25.08096910925416 26.634966572733248 23.96209969865537 24-25 25.089620642074085 24.934454563650586 26.14893965199328 23.82698514228204 26-27 24.657996317519203 25.108564141187657 26.565543942644382 23.667895598648762 28-29 24.591091609593306 25.57919408306925 25.865729859130337 23.963984448207107 30-31 24.865727535589183 25.282942585629957 25.855091359978598 23.996238518802258 32-33 24.601368300193553 25.779513204850357 25.87314859410823 23.74596990084786 34-35 24.442942625462095 25.79242213564634 25.76561510939066 23.999020129500906 36-37 24.739455950857817 25.632139061631143 25.96727364125802 23.66113134625302 38-39 24.68421406255249 25.343295378028905 25.94025720978387 24.032233349634733 40-41 24.32492579435296 25.814180747614756 25.515319944050656 24.345573513981627 42-43 24.22942962616074 25.690738988347473 25.6495752040862 24.43025618140559 44-45 24.459751786056298 25.394286677844008 26.05444123316304 24.091520302936654 46-47 23.962819358284403 25.524567630482604 26.144906648414697 24.367706362818296 48-49 24.054619024506923 25.13622425706919 25.851811960701877 24.95734475772201 50-51 24.100224908089615 25.35610336876177 26.44383518282695 24.09983654032167 52-53 24.198466802605772 25.630418632052177 26.267265119203227 23.90384944613882 54-55 24.20826706048449 25.43386204551499 25.72283175040358 24.63503914359694 56-57 24.109860869358535 26.137252921894845 25.973674480495042 23.77921172825158 58-59 24.177049202096757 26.104074335965898 25.984320794616682 23.73455566732066 60-61 24.764723373826687 25.679908404817937 25.531290627818827 24.024077593536557 62-63 24.228369797759754 26.107445880927884 25.956435210799526 23.70774911051283 64-65 24.622001724657483 25.674794314777937 25.751277941452866 23.95192601911171 66-67 24.73085203735535 25.875717250413988 25.894228009394276 23.49920270283639 68-69 24.483574916965985 25.453094433698897 26.402634193796402 23.660696455538716 70-71 24.286128977132243 26.210270594948714 25.76978796054196 23.73381246737708 72-73 24.20585290964745 25.73586659358738 26.115754704462326 23.942525792302842 74-75 24.375388378132918 25.777005226175493 25.965826707668775 23.881779688022817 76-77 24.140658553533456 25.52402127779441 26.172388936803127 24.162931231869006 78-79 24.558672180249804 25.49618912056238 25.54769596976831 24.39744272941951 80-81 24.19450735398463 25.32286909550512 26.24489934796302 24.237724202547234 82-83 24.085203119124554 26.124477251337876 26.018925041798646 23.77139458773892 84-85 24.175666800428214 26.029520182014853 25.637235231145 24.15757778641193 86-87 23.94217570925368 26.027580488518076 25.908480992915646 24.121762809312592 88-89 23.965773481499344 27.377207843358054 25.05581522024919 23.60120345489341 90-91 23.86500363329925 26.309854599962712 25.338175740119738 24.486966026618294 92-93 23.73153286890225 26.77985234992996 25.303736746281874 24.184878034885912 94-95 23.71118277182484 26.937943294432227 24.93947651618448 24.411397417558454 96-97 23.768342020016284 26.416545435521233 25.072639099793015 24.742473444669468 98-99 24.15793162452861 26.19159842473784 24.989317038042895 24.661152912690653 100-101 24.48762401216731 27.090671264198484 24.265220803716247 24.15648391991796 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4855.0 1 3253.0 2 3193.5 3 3956.5 4 3350.5 5 2738.5 6 1831.0 7 1747.0 8 1848.0 9 1888.0 10 1817.5 11 1716.5 12 1606.0 13 1547.5 14 1564.5 15 1615.5 16 1710.5 17 1808.0 18 1945.5 19 2154.5 20 2350.5 21 2622.5 22 3064.0 23 3614.5 24 4266.0 25 5264.5 26 6448.5 27 7631.0 28 8964.5 29 10491.5 30 12756.0 31 16397.0 32 25190.0 33 40020.5 34 58133.5 35 90254.5 36 128796.5 37 183234.5 38 256674.5 39 308542.5 40 356530.0 41 420829.0 42 501702.0 43 583711.5 44 654925.5 45 761573.5 46 881664.0 47 945553.5 48 980350.0 49 988452.5 50 944402.5 51 865834.5 52 769487.5 53 689672.5 54 624853.5 55 575103.0 56 539973.0 57 451234.0 58 337888.0 59 263275.0 60 203398.0 61 153473.5 62 114572.0 63 78570.0 64 47627.0 65 29122.5 66 18925.5 67 13370.5 68 10816.5 69 8380.0 70 6322.0 71 4846.5 72 3990.0 73 3303.0 74 2622.0 75 2030.0 76 1472.5 77 1130.5 78 894.5 79 722.0 80 567.0 81 382.0 82 251.5 83 188.5 84 144.5 85 111.5 86 90.0 87 78.0 88 69.5 89 65.0 90 55.5 91 44.0 92 42.0 93 32.5 94 19.5 95 15.5 96 9.5 97 4.0 98 4.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12620147823688369 2 0.0 3 0.0 4 0.3413215571841735 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.6512789600360883E-5 12-13 0.0010953836880108264 14-15 0.0 16-17 0.0 18-19 0.0 20-21 8.265167827718053E-4 22-23 4.182374081495882E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0020646322846749514 38-39 0.007388860877309392 40-41 0.006764824109594133 42-43 0.001440595516959693 44-45 0.0 46-47 0.0 48-49 1.3277378036494866E-5 50-51 0.0011484932001568058 52-53 0.012045901223609965 54-55 0.009483367262566458 56-57 0.00881617901623259 58-59 0.012381155019031462 60-61 0.002748417253554437 62-63 2.7218624974814474E-4 64-65 0.0034122861553791805 66-67 0.011312326087093625 68-69 0.019726864417722246 70-71 0.02538966615028731 72-73 0.018983331247678533 74-75 0.0038172461854922733 76-77 0.014349526312941827 78-79 0.007571424825311197 80-81 0.005327547937143564 82-83 0.0034985891126163965 84-85 0.010535599471958675 86-87 0.015122933583567652 88-89 0.012686534713870844 90-91 0.030454985871210098 92-93 0.013702254133662701 94-95 0.014067382029666311 96-97 0.00716314545068898 98-99 0.012274935994739503 100-101 0.1390207867311195 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5063215E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.803022886985346 #Duplication Level Percentage of deduplicated Percentage of total 1 76.83166433636531 16.751625359709486 2 9.42748739275811 4.110954467821418 3 3.997623436152003 2.6148082581591336 4 1.9737727056887164 1.7213684590335232 5 1.3435901699384674 1.4647163612948468 6 0.8977030056061399 1.174358350616772 7 0.6524026080468152 0.9957044296341551 8 0.4555343827123643 0.7945621257669138 9 0.3137632292892665 0.6156888182359478 >10 3.0850296639827715 13.961453309051583 >50 0.451762323477661 6.89028725729212 >100 0.4494283333430169 21.299848376316426 >500 0.08165083552627313 12.49420947022525 >1k 0.03768115515940963 13.657646509088156 >5k 7.539687188495528E-4 1.0137744569531784 >10k+ 1.524532347600049E-4 0.4389939908009728 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTTTGAGTAGCGAAAGACAGGTGAGAATCCTGTCCACCGTAAGACTAAG 16107 0.10692936401691139 No Hit CTTTGTGTTTGAGGGGTCCATCCCGGATTACCAATCTCAGATAAACTCCG 15357 0.10195034725322583 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.7260591447443324E-4 0.0 0.0 2 1.7260591447443324E-4 0.0 0.0 3 1.7924460349268067E-4 0.0 0.0 4 1.7924460349268067E-4 0.0 0.0 5 1.858832925109281E-4 0.0 0.0 6 2.1243804858391786E-4 0.0 0.0 7 2.3899280465690758E-4 0.0 0.0 8 2.522701826934024E-4 0.0 0.0 9 2.7882493876639215E-4 0.0 0.0 10-11 3.1201838385762934E-4 0.0 0.0 12-13 4.381534752043306E-4 0.0 0.0 14-15 5.709272555692792E-4 0.0 0.0 16-17 7.534912035710836E-4 0.0 0.0 18-19 0.0010090807307736097 1.3277378036494866E-5 0.0 20-21 0.001204922056811909 1.3277378036494866E-5 0.0 22-23 0.0015335371632151568 1.3277378036494866E-5 0.0 24-25 0.0018687909586366522 1.3277378036494866E-5 0.0 26-27 0.002174170653476034 1.3277378036494866E-5 0.0 28-29 0.0026023660951529936 1.3277378036494866E-5 0.0 30-31 0.003140099905631036 1.3277378036494866E-5 0.0 32-33 0.0037143465057094386 1.3277378036494866E-5 0.0 34-35 0.004461199020262275 1.3277378036494866E-5 0.0 36-37 0.005383976793798668 1.3277378036494866E-5 0.0 38-39 0.006668563118829546 1.3277378036494866E-5 0.0 40-41 0.008255209794190683 1.3277378036494866E-5 0.0 42-43 0.010462573892757954 1.3277378036494866E-5 0.0 44-45 0.012819308494235793 1.3277378036494866E-5 0.0 46-47 0.015949450366339456 1.3277378036494866E-5 0.0 48-49 0.01983640278652333 1.3277378036494866E-5 0.0 50-51 0.02519714416875813 1.3277378036494866E-5 0.0 52-53 0.0314906213580567 1.3277378036494866E-5 0.0 54-55 0.03957986392679119 1.3277378036494866E-5 0.0 56-57 0.050115463398749865 1.3277378036494866E-5 0.0 58-59 0.06286838500280319 1.3277378036494866E-5 0.0 60-61 0.07893401242696196 1.3277378036494866E-5 0.0 62-63 0.09892642440541412 1.3277378036494866E-5 0.0 64-65 0.12431940990021055 1.3277378036494866E-5 0.0 66-67 0.15551460959695523 1.3277378036494866E-5 0.0 68-69 0.19397585442417173 1.3277378036494866E-5 0.0 70-71 0.24121012678900222 1.3277378036494866E-5 0.0 72-73 0.300473703654897 1.3277378036494866E-5 0.0 74-75 0.3717931397779292 1.3277378036494866E-5 0.0 76-77 0.458315173752748 1.3277378036494866E-5 0.0 78-79 0.5662038283327961 1.3277378036494866E-5 0.0 80-81 0.6963984780141557 1.3277378036494866E-5 0.0 82-83 0.8516807334954722 1.3277378036494866E-5 0.0 84-85 1.0431803569158378 1.3277378036494866E-5 0.0 86-87 1.2670834214342688 1.3277378036494866E-5 0.0 88-89 1.5287440297439823 1.3277378036494866E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 150775 0.0 78.61767 1 TTTGTGT 160910 0.0 73.645256 2 TTGTGTT 183585 0.0 66.59187 3 TGTGTTT 189985 0.0 64.63955 4 GTGTTTG 206265 0.0 59.65202 5 ACTTTGT 22770 0.0 49.415276 1 TTTGAGT 76415 0.0 41.935635 3 GTTTGAA 27310 0.0 40.985558 2 TGTTTGA 355325 0.0 40.72582 1 TTGAGTC 28540 0.0 40.32113 4 GTTTGAG 199935 0.0 40.301785 2 TTGAGGG 41380 0.0 39.27891 9 TGAGTCT 5075 0.0 39.015125 5 TTTGAGG 76490 0.0 38.587746 8 TTTGATT 36575 0.0 37.61248 3 TGAGTGG 11045 0.0 36.111584 5 TTGATTC 10735 0.0 34.93351 4 GTTTGAT 96980 0.0 34.158367 2 TTGAGTG 32955 0.0 33.79146 4 TGAATGC 2965 0.0 33.46991 5 >>END_MODULE