FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l04n01_30082.34100000035d38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l04n01_30082.34100000035d38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15063215
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGAGTAGCGAAAGACAGGTGAGAATCCTGTCCACCGTAAGACTAAG161070.10692936401691139No Hit
CTTTGTGTTTGAGGGGTCCATCCCGGATTACCAATCTCAGATAAACTCCG153570.10195034725322583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1507750.078.617671
TTTGTGT1609100.073.6452562
TTGTGTT1835850.066.591873
TGTGTTT1899850.064.639554
GTGTTTG2062650.059.652025
ACTTTGT227700.049.4152761
TTTGAGT764150.041.9356353
GTTTGAA273100.040.9855582
TGTTTGA3553250.040.725821
TTGAGTC285400.040.321134
GTTTGAG1999350.040.3017852
TTGAGGG413800.039.278919
TGAGTCT50750.039.0151255
TTTGAGG764900.038.5877468
TTTGATT365750.037.612483
TGAGTGG110450.036.1115845
TTGATTC107350.034.933514
GTTTGAT969800.034.1583672
TTGAGTG329550.033.791464
TGAATGC29650.033.469915
Produced by FastQC (version 0.11.2)