##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l04n01_30076.34100000035ce6.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16088559 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.72914727788859 33.0 33.0 33.0 33.0 33.0 2 32.76430070586185 33.0 33.0 33.0 33.0 33.0 3 32.791918592584956 33.0 33.0 33.0 33.0 33.0 4 36.59814940542531 37.0 37.0 37.0 37.0 37.0 5 36.6193785285556 37.0 37.0 37.0 37.0 37.0 6 36.635531808659806 37.0 37.0 37.0 37.0 37.0 7 36.61101009729958 37.0 37.0 37.0 37.0 37.0 8 36.562280873010444 37.0 37.0 37.0 37.0 37.0 9 36.58343534682006 37.0 37.0 37.0 37.0 37.0 10-11 36.57204237495726 37.0 37.0 37.0 37.0 37.0 12-13 36.544113024665535 37.0 37.0 37.0 37.0 37.0 14-15 38.60417819271446 40.0 37.0 40.0 37.0 40.0 16-17 38.582213142892414 40.0 37.0 40.0 37.0 40.0 18-19 38.53742485576241 40.0 37.0 40.0 37.0 40.0 20-21 38.500170183047466 40.0 37.0 40.0 37.0 40.0 22-23 38.46342220580476 40.0 37.0 40.0 37.0 40.0 24-25 38.419518180590316 40.0 37.0 40.0 37.0 40.0 26-27 38.318643049386836 40.0 37.0 40.0 37.0 40.0 28-29 38.262322374552 40.0 37.0 40.0 37.0 40.0 30-31 38.19933320317873 40.0 37.0 40.0 37.0 40.0 32-33 38.09946866590103 40.0 37.0 40.0 37.0 40.0 34-35 38.014119692136504 40.0 37.0 40.0 37.0 40.0 36-37 37.94060860267224 40.0 37.0 40.0 35.0 40.0 38-39 37.8360504194316 40.0 37.0 40.0 33.0 40.0 40-41 37.6720517978024 40.0 37.0 40.0 33.0 40.0 42-43 37.58507138519988 40.0 37.0 40.0 33.0 40.0 44-45 37.48763279545421 40.0 37.0 40.0 33.0 40.0 46-47 37.35861089237389 40.0 37.0 40.0 33.0 40.0 48-49 37.19274734300319 40.0 37.0 40.0 33.0 40.0 50-51 37.02565450392419 37.0 37.0 40.0 33.0 40.0 52-53 36.8188104975716 37.0 37.0 40.0 33.0 40.0 54-55 36.60417290945696 37.0 37.0 40.0 33.0 40.0 56-57 36.388412504811654 37.0 37.0 40.0 33.0 40.0 58-59 36.18443506966659 37.0 37.0 40.0 33.0 40.0 60-61 35.89868514638259 37.0 37.0 40.0 33.0 40.0 62-63 35.57932174037464 37.0 35.0 38.5 33.0 40.0 64-65 35.3417099070215 37.0 33.0 37.0 33.0 40.0 66-67 35.05794219979552 37.0 33.0 37.0 27.0 40.0 68-69 34.787271905457786 37.0 33.0 37.0 27.0 40.0 70-71 34.47984443479369 37.0 33.0 37.0 27.0 37.0 72-73 34.208468204020015 37.0 33.0 37.0 27.0 37.0 74-75 33.92501525462908 37.0 33.0 37.0 27.0 37.0 76-77 31.197663383028896 33.0 30.0 35.0 24.5 37.0 78-79 33.583127301829826 35.0 33.0 37.0 27.0 37.0 80-81 33.99643846288534 37.0 33.0 37.0 27.0 37.0 82-83 33.961888476152524 37.0 33.0 37.0 27.0 37.0 84-85 33.9045578600296 37.0 33.0 37.0 27.0 37.0 86-87 33.81564184834701 37.0 33.0 37.0 27.0 37.0 88-89 33.630408882485995 37.0 33.0 37.0 27.0 37.0 90-91 33.500348943618874 37.0 33.0 37.0 27.0 37.0 92-93 33.392610301519234 37.0 33.0 37.0 27.0 37.0 94-95 33.28189379794673 37.0 33.0 37.0 27.0 37.0 96-97 33.16310718069903 37.0 33.0 37.0 27.0 37.0 98-99 33.02119173631399 35.0 33.0 37.0 27.0 37.0 100-101 31.267618001090092 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11562246542142418 2306 2 0.10094935966758811 2306 3 0.0928395096849286 2306 4 -0.07358518194181585 2306 5 0.0032070251443059306 2306 6 0.07754054883957195 2306 7 0.10813035484674316 2306 8 0.17096642549864782 2306 9 0.13459276081931648 2306 10-11 0.10365844160553905 2306 12-13 0.1189781139153041 2306 14-15 0.20909989078173652 2306 16-17 0.2216544683728472 2306 18-19 0.1732280412485423 2306 20-21 0.14669437518100636 2306 22-23 0.15271069134006865 2306 24-25 0.16423576782917593 2306 26-27 0.1875539846190648 2306 28-29 0.1264989638972409 2306 30-31 0.13321125890212926 2306 32-33 0.07803216831729998 2306 34-35 0.1108889668868045 2306 36-37 0.048430395907708146 2306 38-39 0.07762504679102022 2306 40-41 0.15105441264305597 2306 42-43 0.1298310111067451 2306 44-45 0.034371105249114464 2306 46-47 0.07386998257204169 2306 48-49 0.09708405212396798 2306 50-51 0.022887783314040178 2306 52-53 0.043742682886971807 2306 54-55 0.07163560863056517 2306 56-57 0.01744575199948173 2306 58-59 -0.0457823300800726 2306 60-61 -0.12212451651376455 2306 62-63 -0.09423268019722997 2306 64-65 -0.13266736704929372 2306 66-67 -0.15412884118762094 2306 68-69 -0.25396012066838125 2306 70-71 -0.22377813601035967 2306 72-73 -0.16659298316893256 2306 74-75 -0.30592662191013176 2306 76-77 -0.8461179204324125 2306 78-79 -0.07527926622375958 2306 80-81 -3.7080157453317497E-4 2306 82-83 -0.16092376187329194 2306 84-85 -0.17972883713416365 2306 86-87 -0.20495643152398912 2306 88-89 -0.2146090946761916 2306 90-91 -0.2783330749669375 2306 92-93 -0.31717099200124466 2306 94-95 -0.30395112809861047 2306 96-97 -0.2460377197799133 2306 98-99 -0.15711934796637195 2306 100-101 -0.502585781683635 2307 1 0.10972478078551973 2307 2 0.09960182575625254 2307 3 0.05295683035843268 2307 4 -0.11217404566502864 2307 5 0.009132521731771703 2307 6 0.06404246777034217 2307 7 0.10044934617771162 2307 8 0.1647579063132838 2307 9 0.08765206332074627 2307 10-11 0.0844111175843878 2307 12-13 0.0820333405992244 2307 14-15 0.14739392278133323 2307 16-17 0.13524867419540243 2307 18-19 0.07521761813027439 2307 20-21 0.08146943724238298 2307 22-23 0.07566289209548671 2307 24-25 0.10841321684311822 2307 26-27 0.12407814650408966 2307 28-29 0.08376281241829275 2307 30-31 0.07029807903109742 2307 32-33 0.07620426448684015 2307 34-35 0.12650536725872996 2307 36-37 0.0838082345532527 2307 38-39 0.028603797632406724 2307 40-41 0.043768495035912736 2307 42-43 -0.06347454296223987 2307 44-45 -0.05213050767368088 2307 46-47 -0.020204976375076455 2307 48-49 -0.04657554447198464 2307 50-51 -0.14319530181379747 2307 52-53 -0.14503351856726 2307 54-55 -0.028095399932404064 2307 56-57 -0.19507205688192641 2307 58-59 -0.1715739022798175 2307 60-61 -0.2424650331407605 2307 62-63 -0.27836082228618864 2307 64-65 -0.2755909936151184 2307 66-67 -0.28736247503817225 2307 68-69 -0.3878171588221164 2307 70-71 -0.3714954260969776 2307 72-73 -0.29511707091658224 2307 74-75 -0.4000660998556711 2307 76-77 -0.8880489127839155 2307 78-79 -0.3056732782598175 2307 80-81 -0.17778513229633575 2307 82-83 -0.16435815190430247 2307 84-85 -0.2026689783936959 2307 86-87 -0.22767442142996686 2307 88-89 -0.28530714858025874 2307 90-91 -0.3657653068072051 2307 92-93 -0.35171963250694915 2307 94-95 -0.22283397576426012 2307 96-97 -0.2309740010966621 2307 98-99 -0.19996667770432452 2307 100-101 -0.6545521210852101 2201 1 0.10608794767214391 2201 2 0.06657523526169484 2201 3 0.060063885529608285 2201 4 -0.1951837080821619 2201 5 -0.059838806681270285 2201 6 0.010718299290289224 2201 7 0.07070117664986242 2201 8 0.09770313962721389 2201 9 0.08388390838844373 2201 10-11 0.05416540782066903 2201 12-13 0.05172631425715224 2201 14-15 0.10879404176255036 2201 16-17 0.10391056643467067 2201 18-19 0.10615276583317979 2201 20-21 0.06786429005054373 2201 22-23 0.05458919887527003 2201 24-25 0.08644740927302763 2201 26-27 0.1663508841260395 2201 28-29 0.0719225457117929 2201 30-31 0.03919126101082071 2201 32-33 0.12551289913313468 2201 34-35 0.07522261896466631 2201 36-37 0.018800169523153443 2201 38-39 0.10815915003028209 2201 40-41 0.11437662184632558 2201 42-43 0.09433321030655151 2201 44-45 -9.732619203433046E-4 2201 46-47 -0.015588723636646762 2201 48-49 -0.060786949671161494 2201 50-51 -0.0024946645818957336 2201 52-53 -0.051909345185748634 2201 54-55 -0.05002993733569383 2201 56-57 -0.07427879683628902 2201 58-59 -0.15050979943620035 2201 60-61 -0.15580687007015115 2201 62-63 0.018612048444907714 2201 64-65 -0.06999167896957204 2201 66-67 -0.1817528099996224 2201 68-69 -0.22572305790527025 2201 70-71 -0.20903467844886592 2201 72-73 -0.13464912816732522 2201 74-75 -0.3569672404953863 2201 76-77 -0.8677642132121264 2201 78-79 -0.11378070825433184 2201 80-81 -0.035726355396548115 2201 82-83 -0.04598043656451978 2201 84-85 -0.15184213045323958 2201 86-87 -0.05051130120062197 2201 88-89 -0.043082359620875366 2201 90-91 -0.17008942018605921 2201 92-93 -0.2521251882140376 2201 94-95 -0.16103213336209876 2201 96-97 -0.07103747406056726 2201 98-99 -0.21119509255386504 2201 100-101 -0.6596637789286532 1101 1 0.079528660889153 1101 2 0.06779273253970075 1101 3 0.05950120312523666 1101 4 -0.14707184776342075 1101 5 -0.05313725552189652 1101 6 0.05245607498742544 1101 7 0.07560779653001504 1101 8 0.1473517369096342 1101 9 0.07993503120570011 1101 10-11 0.05756021656234367 1101 12-13 0.06911580808595374 1101 14-15 0.11269409565139199 1101 16-17 0.07916664803904183 1101 18-19 0.09220452522676936 1101 20-21 0.1045201169924681 1101 22-23 0.08194498914682669 1101 24-25 0.0850408362392514 1101 26-27 0.13964753582501999 1101 28-29 0.0826960458699233 1101 30-31 0.08282205783208951 1101 32-33 0.09790538314489794 1101 34-35 0.07829410119005331 1101 36-37 0.09943938363750959 1101 38-39 0.0924475587367013 1101 40-41 0.12245083391498923 1101 42-43 0.07069031008374083 1101 44-45 0.028718366290171105 1101 46-47 0.05927450450410987 1101 48-49 0.06987596658325401 1101 50-51 -0.03613937205262374 1101 52-53 -0.03024391247794256 1101 54-55 0.021044686530650836 1101 56-57 0.021427332465798088 1101 58-59 -0.018757643828081427 1101 60-61 -0.09193476064659478 1101 62-63 -0.060147365427582145 1101 64-65 -0.14555296910333482 1101 66-67 -0.12683004994583058 1101 68-69 -0.12183043064166554 1101 70-71 -0.2132391495053909 1101 72-73 -0.0721404924105471 1101 74-75 -0.15666311410436862 1101 76-77 -0.6583165701488056 1101 78-79 -0.01830553068538876 1101 80-81 -0.04064790122134809 1101 82-83 0.00935258454637733 1101 84-85 -0.027043538360778996 1101 86-87 0.03337380794945943 1101 88-89 0.038923052714004314 1101 90-91 0.001865074376382836 1101 92-93 -0.08852393312028539 1101 94-95 -0.14540279876922568 1101 96-97 -0.03299016846721514 1101 98-99 -0.07277232808819178 1101 100-101 -0.24255474806193433 2304 1 0.06111754556537363 2304 2 0.05669435750316154 2304 3 0.05271719350085391 2304 4 -0.19600427304155943 2304 5 -0.06441370312730754 2304 6 0.014055610201005209 2304 7 0.044454418406985496 2304 8 0.11719984244832204 2304 9 0.07190862615487958 2304 10-11 0.048488357866538934 2304 12-13 0.037627873015196656 2304 14-15 0.05260717313328911 2304 16-17 0.06982677780032276 2304 18-19 0.055841790858544016 2304 20-21 0.0424044091374256 2304 22-23 0.05487407520431731 2304 24-25 0.040419220158035785 2304 26-27 0.09417247717691879 2304 28-29 0.008407140558936987 2304 30-31 0.027054153259264524 2304 32-33 0.04836708951383173 2304 34-35 0.05207541350154088 2304 36-37 0.02318191956717186 2304 38-39 -0.010877740313723905 2304 40-41 0.020692538562975926 2304 42-43 0.034066360252644756 2304 44-45 -0.03860455699031462 2304 46-47 -0.02496492283527374 2304 48-49 -0.04423106799009702 2304 50-51 -0.14975482804908324 2304 52-53 -0.10924071496046395 2304 54-55 -0.02600264468690483 2304 56-57 -0.12890686122435824 2304 58-59 -0.18165837583244837 2304 60-61 -0.1547705120899252 2304 62-63 -0.08073232367128469 2304 64-65 -0.061901249965622185 2304 66-67 -0.11514699112685634 2304 68-69 -0.2466498054520514 2304 70-71 -0.16151104543892814 2304 72-73 -0.14512794566868337 2304 74-75 -0.1961306343615874 2304 76-77 -0.41610999946737337 2304 78-79 -0.041849358180066076 2304 80-81 0.011700064250469211 2304 82-83 -0.06131624092473942 2304 84-85 -0.09489320965958115 2304 86-87 0.00816598262220225 2304 88-89 -0.009601888659716451 2304 90-91 -0.027877530801291073 2304 92-93 -0.08588258152810369 2304 94-95 -0.0877228012625011 2304 96-97 0.004531957518537411 2304 98-99 0.037702910770576636 2304 100-101 -0.30737424853980144 2202 1 0.0721261785105014 2202 2 0.050878120528921045 2202 3 0.03987779580499762 2202 4 -0.2448341338507376 2202 5 -0.09552056640968942 2202 6 0.028303559143473933 2202 7 0.016014566920333095 2202 8 0.14894734144225907 2202 9 0.0718930852358568 2202 10-11 0.05363674482631353 2202 12-13 0.04894322930366002 2202 14-15 0.05143500664625833 2202 16-17 0.05496720218773277 2202 18-19 0.055213607093499206 2202 20-21 0.05630522025813178 2202 22-23 0.03082876488138453 2202 24-25 0.06985583403747597 2202 26-27 0.06257150973701897 2202 28-29 0.06118975373868096 2202 30-31 0.08928639849234088 2202 32-33 0.03718733962454479 2202 34-35 0.030204214049973643 2202 36-37 0.07419178102346535 2202 38-39 0.014492056713983459 2202 40-41 0.052480623925795555 2202 42-43 0.06141719473023244 2202 44-45 0.002331649407658176 2202 46-47 0.07191466385918943 2202 48-49 0.07871574101534407 2202 50-51 0.0163388755025764 2202 52-53 0.07456753315227616 2202 54-55 -0.018348180894115274 2202 56-57 0.05141887118062982 2202 58-59 0.04985765244656193 2202 60-61 0.021125487109266317 2202 62-63 0.006653844405221321 2202 64-65 -0.03320498342622358 2202 66-67 -0.019141790935144343 2202 68-69 -0.027262979136224885 2202 70-71 -0.06265652846121128 2202 72-73 -0.0010185050406477103 2202 74-75 -0.08134686243797518 2202 76-77 -0.09391217782494721 2202 78-79 0.04893767258428028 2202 80-81 0.14551271073652572 2202 82-83 0.06537577789826798 2202 84-85 0.05703641737857623 2202 86-87 0.03431703691734356 2202 88-89 0.0825255640653566 2202 90-91 0.07402359275558013 2202 92-93 0.1166835609063881 2202 94-95 0.06036170716114242 2202 96-97 0.13239812462436618 2202 98-99 0.16062253075111244 2202 100-101 0.016379667023567634 1102 1 0.07543622018406637 1102 2 0.054741565520210145 1102 3 0.042486410483185466 1102 4 -0.23681645779733884 1102 5 -0.07846294673117882 1102 6 0.0015786784593956327 1102 7 0.06566948485943414 1102 8 0.13280179103391987 1102 9 0.036128163150593195 1102 10-11 0.046197956728271095 1102 12-13 0.013651692452199882 1102 14-15 0.04248245388733807 1102 16-17 0.011999746956952606 1102 18-19 0.032410373778112955 1102 20-21 0.01987225472583276 1102 22-23 0.038614760506135326 1102 24-25 0.0651465211682094 1102 26-27 0.07833379186804024 1102 28-29 0.08378773410384355 1102 30-31 0.07574344953307843 1102 32-33 0.0510003275010078 1102 34-35 0.08947830345781682 1102 36-37 0.02994095417536613 1102 38-39 -0.009680649371809125 1102 40-41 0.07428743514741853 1102 42-43 0.05064743622086354 1102 44-45 -0.0013202244360641657 1102 46-47 -0.021248241105489285 1102 48-49 -0.0296871541810404 1102 50-51 -0.004087656363459757 1102 52-53 -0.07452759575966894 1102 54-55 -0.06866097356761003 1102 56-57 0.0012973913815201854 1102 58-59 -0.07047464655548907 1102 60-61 -0.09805011151898668 1102 62-63 -7.455213073654932E-4 1102 64-65 0.017568371846202524 1102 66-67 -0.047766884608130056 1102 68-69 -0.13731613440534574 1102 70-71 -0.19090566378307727 1102 72-73 -0.09465529575955856 1102 74-75 -0.16267811639994534 1102 76-77 -0.17602612234726678 1102 78-79 -0.04987323289441292 1102 80-81 -0.06675478879613195 1102 82-83 -0.13087096377196872 1102 84-85 -0.1397713355198391 1102 86-87 -0.09138771097284604 1102 88-89 -0.16565265100431503 1102 90-91 -0.17880762547154916 1102 92-93 -0.18811385519766333 1102 94-95 -0.27243682591825547 1102 96-97 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40-41 -0.2507259481709596 1213 42-43 -0.2724923774342187 1213 44-45 -0.2505754545447658 1213 46-47 -0.24686650086612616 1213 48-49 -0.2282664239508918 1213 50-51 -0.17385550256403803 1213 52-53 -0.20350887005646712 1213 54-55 -0.1339456359644302 1213 56-57 -0.09355213406077212 1213 58-59 -0.1550337746108923 1213 60-61 -0.1667374314077108 1213 62-63 -0.046192234806113674 1213 64-65 -0.08152180256982433 1213 66-67 0.023801662916710598 1213 68-69 0.08198752436883439 1213 70-71 -0.018722412471980476 1213 72-73 0.025919470850787718 1213 74-75 0.12850155833576338 1213 76-77 0.507529204140873 1213 78-79 0.0556176326095823 1213 80-81 -0.0874942935702947 1213 82-83 -0.17800795810566683 1213 84-85 -0.11619175066363852 1213 86-87 -0.10074030115491439 1213 88-89 0.0034959622619794573 1213 90-91 0.019646843589541163 1213 92-93 0.15806535203785899 1213 94-95 0.0808471755718827 1213 96-97 0.13722439263099773 1213 98-99 0.18770138615872867 1213 100-101 0.3767819397293337 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 48.0 8 512.0 9 1455.0 10 1089.0 11 425.0 12 382.0 13 785.0 14 1928.0 15 4563.0 16 8455.0 17 12742.0 18 16891.0 19 22119.0 20 27203.0 21 32780.0 22 40097.0 23 48467.0 24 58909.0 25 72229.0 26 89849.0 27 112462.0 28 142329.0 29 180863.0 30 236224.0 31 310322.0 32 416137.0 33 578385.0 34 885181.0 35 1634753.0 36 3655523.0 37 6100285.0 38 1395133.0 39 33.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.211649112928356 24.810436864761005 32.59683120997604 23.381082812334597 2 24.93757831263819 24.870679841494816 30.84403022048152 19.347711625385468 3 21.086189260330897 23.004415746618452 36.0985405840262 19.81085440902445 4 20.993636180950155 23.281041003392033 35.31935165061751 20.405971165040295 5 25.667339132112453 22.529500622150188 32.486203394598604 19.31695685113875 6 27.09504934531427 22.882260617622745 31.247397607206462 18.77529242985652 7 25.855404452319192 26.721914622683112 28.399404819288044 19.023276105709655 8 25.140449185038882 24.076892156718323 30.99497599505338 19.78768266318941 9 22.284606098035255 23.348517415388166 34.06988780039281 20.296988686183763 10-11 25.411682404044367 24.170296835234737 30.046113526043822 20.37190723467707 12-13 24.88319833738429 26.688939050684024 27.639730628010867 20.78813198392082 14-15 24.619671465263433 25.181350161442378 28.630801735954265 21.568176637339924 16-17 24.223527414729933 25.115903792253864 28.714258996097787 21.94630979691842 18-19 23.655524400911233 25.45765285753684 29.23148679754352 21.65533594400841 20-21 22.871079772064544 26.143443971543746 29.295423402207327 21.690052854184383 22-23 22.812827077631926 25.835670044255842 29.23147202625578 22.120030851856455 24-25 23.080099964204376 26.165963651561334 28.66615338266155 22.087783001572735 26-27 22.900624599132836 26.043572329877403 28.784840208498473 22.270962862491288 28-29 23.09041163972485 26.09183644103863 28.513681679011775 22.304070240224746 30-31 22.843907275971702 25.82335994168278 28.734761142996092 22.597971639349428 32-33 22.946405579269094 26.196320627596293 28.29938032362003 22.55789346951458 34-35 22.656942116444363 26.345659670328462 28.260803220474873 22.736594992752305 36-37 22.827955322363653 26.462616019613844 28.479780371088726 22.229648286933777 38-39 23.083896502779353 26.422418871758175 28.106987022458785 22.386697603003682 40-41 22.720231399706407 27.009636308664227 28.14834112075173 22.121791170877643 42-43 22.758549895186515 26.934990591478343 27.943463155817717 22.36299635751742 44-45 22.57435547832469 26.582955627039066 28.612811128703324 22.22987776593292 46-47 22.595441891346514 26.803453932698385 28.46294686801969 22.138157307935412 48-49 22.599742083776 26.508184465885147 28.44420017145726 22.44787327888159 50-51 22.54623449927945 26.74524104842226 28.398417116966208 22.310107335332084 52-53 22.829096500841832 27.231305943204514 27.68894222354782 22.250655332405838 54-55 22.61061668963176 26.874599582006585 28.03511982632291 22.479663902038748 56-57 22.721379561304666 27.14644704196833 27.867022983053424 22.265150413673577 58-59 22.705842023496974 26.934618641142695 28.004923841552486 22.354615493807852 60-61 22.710824984586473 26.951071978910186 27.76840372440747 22.56969931209587 62-63 22.546922175014327 27.24531643773388 27.679660075811157 22.528101311440633 64-65 22.882154055893842 27.206994408676177 27.406036548679406 22.50481498675057 66-67 23.187370744494924 27.245539039708174 27.016581691406248 22.550508524390654 68-69 22.855062456103706 27.4391575392679 27.116108780595688 22.589671224032703 70-71 22.991730256742187 27.60033767706418 27.109141083005582 22.298790983188045 72-73 23.066352245195283 27.494525780856232 26.882703208190044 22.556418765758437 74-75 23.061904468429418 27.663855843276387 26.888594321335535 22.385645366958666 76-77 23.155578159953453 27.367571950837693 27.047816351858845 22.42903353735001 78-79 23.27748654584297 27.255694469593518 27.094338447457694 22.372480537105822 80-81 23.228865803541606 27.375302264295385 26.980159801170355 22.415672130992654 82-83 22.999178426062 27.520029264006812 27.0490764169431 22.43171589298809 84-85 22.7365422540355 27.900733048733677 26.904461231714578 22.45826346551625 86-87 22.570500819460364 27.596138950439247 26.99329599117156 22.840064238928832 88-89 22.31537166861187 28.02854554185711 26.899200109408 22.756882680123024 90-91 22.339635421276313 28.025991873249577 26.92704424605223 22.707328459421877 92-93 22.460416407454943 28.536061587058636 26.4703393042764 22.533182701210023 94-95 22.39070739403723 28.68623464780549 26.184198919570534 22.73885903858674 96-97 22.427471459136495 28.711468435663555 26.110044718059903 22.751015387140043 98-99 22.759798082643588 28.7086698837588 25.8318116033945 22.69972043020311 100-101 23.075412953750867 28.907750676299017 25.319229038262858 22.697607331687262 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9141.0 1 5275.5 2 2179.0 3 2638.0 4 2502.0 5 1992.0 6 1104.5 7 892.0 8 910.5 9 942.5 10 919.5 11 895.0 12 846.0 13 794.5 14 794.0 15 811.5 16 826.5 17 856.5 18 921.0 19 975.0 20 1073.0 21 1219.5 22 1414.5 23 1664.0 24 1952.0 25 2351.0 26 2949.0 27 3754.5 28 4731.5 29 5945.0 30 7949.5 31 12301.5 32 41648.0 33 93284.0 34 148358.5 35 258276.0 36 368237.5 37 538834.0 38 752568.0 39 775576.5 40 736505.5 41 709993.0 42 722128.0 43 849103.5 44 960255.5 45 1028587.0 46 1057123.5 47 970592.0 48 849417.5 49 808982.0 50 731957.0 51 632701.0 52 578066.5 53 509133.0 54 426809.5 55 353840.5 56 295069.5 57 221767.0 58 155772.0 59 114154.0 60 84782.0 61 64464.0 62 48724.5 63 34318.0 64 23573.0 65 16680.0 66 11327.5 67 8240.0 68 6669.5 69 4633.0 70 3183.5 71 2562.5 72 2300.5 73 1982.5 74 1538.0 75 1162.5 76 947.0 77 816.5 78 727.5 79 618.5 80 449.5 81 268.0 82 165.0 83 131.5 84 118.0 85 87.0 86 57.5 87 54.5 88 53.5 89 47.5 90 46.0 91 39.5 92 27.5 93 20.0 94 14.5 95 15.5 96 10.5 97 4.5 98 3.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12370281266333423 2 0.0 3 0.0 4 0.34251047592267275 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.486238823501844E-5 12-13 0.0010877294852820567 14-15 9.323395588131914E-6 16-17 0.0 18-19 0.0 20-21 9.012615735194184E-4 22-23 3.853670176427858E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0020573626264477757 38-39 0.006914851727864503 40-41 0.0066569044499261865 42-43 0.0013581079573378823 44-45 0.0 46-47 0.0 48-49 9.323395588131914E-6 50-51 0.0012058258293983944 52-53 0.011657352283694269 54-55 0.00909652629548737 56-57 0.008440780805788759 58-59 0.012011641316043284 60-61 0.0025856883764419176 62-63 1.8957571029201557E-4 64-65 0.0031606311043767188 66-67 0.010690826941057928 68-69 0.019029050395377237 70-71 0.024616872151197632 72-73 0.018221022777739137 74-75 0.0035335669279019954 76-77 0.013739577298377064 78-79 0.00716036781168531 80-81 0.005078142797002516 82-83 0.003334667822021848 84-85 0.010156285594005032 86-87 0.014687455849837144 88-89 0.012331744564569145 90-91 0.02960178099231883 92-93 0.013223682742500431 94-95 0.01353757039396754 96-97 0.006830941167571315 98-99 0.011675999074870535 100-101 0.14063099125285242 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6088559E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.31988089636342 #Duplication Level Percentage of deduplicated Percentage of total 1 73.6937894050751 9.815924976772887 2 9.52313791812024 2.5369412565800777 3 4.043560310099609 1.615792251833674 4 2.6394854118285616 1.4063052525298076 5 1.577367829702987 1.050517581069952 6 1.337785069474698 1.0691482674201744 7 0.6960791344137572 0.6490183815384494 8 0.7465761804469775 0.7955444642892532 9 0.4196735365295441 0.5031001369736234 >10 3.991925665512481 10.622508490642629 >50 0.6323591856466856 5.868284275366819 >100 0.4924333322739829 13.47335852569976 >500 0.07096053567522664 6.430474463794221 >1k 0.12725348465716652 37.200230893698354 >5k 0.007150270805917326 6.149414086706216 >10k+ 4.627297368972543E-4 0.8134366950840025 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT 18855 0.11719508254281817 No Hit CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC 18732 0.11643056410459135 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.367431352926014E-4 0.0 0.0 2 1.367431352926014E-4 0.0 0.0 3 1.4295873235135601E-4 0.0 0.0 4 1.4917432941011062E-4 0.0 0.0 5 1.6160552352761985E-4 0.0 0.0 6 1.6782112058637445E-4 0.0 0.0 7 1.7403671764512906E-4 0.0 0.0 8 2.2997709117392054E-4 0.0 0.0 9 3.0456425587897584E-4 0.0 0.0 10-11 4.040138088190496E-4 6.215597058754609E-6 0.0 12-13 6.495298926398566E-4 1.2431194117509219E-5 0.0 14-15 9.88279932341983E-4 1.2431194117509219E-5 0.0 16-17 0.001519713480865502 1.2431194117509219E-5 0.0 18-19 0.0020853328132121717 1.2431194117509219E-5 0.0 20-21 0.0027814796837926877 1.2431194117509219E-5 0.0 22-23 0.003629908682312692 1.2431194117509219E-5 0.0 24-25 0.004695883577889108 1.2431194117509219E-5 0.0 26-27 0.005883062616111238 1.8646791176263827E-5 0.0 28-29 0.007200769192567215 1.8646791176263827E-5 0.0 30-31 0.008760884054314622 2.4862388235018437E-5 0.0 32-33 0.010252627348415727 2.4862388235018437E-5 0.0 34-35 0.011992994524867018 2.7970186764395742E-5 0.0 36-37 0.013975769986609739 3.1077985293773044E-5 0.0 38-39 0.016421607429229677 3.1077985293773044E-5 0.0 40-41 0.01915957793361108 3.1077985293773044E-5 0.0 42-43 0.022575048517396744 3.7293582352527654E-5 0.0 44-45 0.026907319667348703 4.3509179411282264E-5 0.0 46-47 0.03261945336434419 4.6616977940659566E-5 0.0 48-49 0.03974252759367697 5.283257499941418E-5 0.0 50-51 0.048789329112694305 5.9048172058168787E-5 0.0 52-53 0.06026332128315531 6.215597058754609E-5 0.0 54-55 0.07428260044917634 6.837156764630069E-5 0.0 56-57 0.09144386392839782 8.080276176380993E-5 0.0 58-59 0.11243704299434151 9.634175441069645E-5 0.0 60-61 0.13899628922639995 9.944955294007375E-5 0.0 62-63 0.17167168296427293 9.944955294007375E-5 0.0 64-65 0.21199536888294346 1.1188074705758297E-4 0.0 66-67 0.2619345834515074 1.1188074705758297E-4 0.0 68-69 0.3229033750008313 1.1188074705758297E-4 0.0 70-71 0.39755891127353293 1.1809634411633757E-4 0.0 72-73 0.48902452979163635 1.367431352926014E-4 0.0 74-75 0.5996839120271741 1.4295873235135601E-4 0.0 76-77 0.7331638588639293 1.4295873235135601E-4 0.0 78-79 0.8944990039194933 1.4295873235135601E-4 0.0 80-81 1.0898521116776214 1.4917432941011062E-4 0.0 82-83 1.322977402761801 1.4917432941011062E-4 0.0 84-85 1.5971536046205257 1.5228212793948795E-4 0.0 86-87 1.9241561658816058 1.6160552352761985E-4 0.0 88-89 2.304650155430328 1.6160552352761985E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTTGT 36305 0.0 57.372375 1 CTTTGTG 106110 0.0 56.173405 1 TTGTGTT 119760 0.0 51.10162 3 TTTGTGT 117520 0.0 50.69712 2 TGTGTTT 128475 0.0 47.894943 4 GTGTTTG 143760 0.0 42.848087 5 TTCCGAT 1260 0.0 38.06228 6 TCCGATC 1430 0.0 33.869446 7 TTGAGGG 39990 0.0 31.917082 9 TTTGAGG 59400 0.0 31.016247 8 GTTTGAA 12950 0.0 30.171036 2 TGTTTGA 211240 0.0 30.170557 1 GTTTGAG 121340 0.0 30.107008 2 TTTGAGT 41670 0.0 27.23732 3 AGTCCTG 3295 0.0 27.092335 7 CTTGTGT 6060 0.0 27.047798 2 GTTTGAC 35890 0.0 26.341576 7 TGAGTCT 2200 0.0 26.330933 5 TTTGATT 29180 0.0 25.718248 3 TTTGAAC 4920 0.0 25.373613 3 >>END_MODULE