FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l04n01_30076.34100000035ce6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l04n01_30076.34100000035ce6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16088559
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT188550.11719508254281817No Hit
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC187320.11643056410459135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT363050.057.3723751
CTTTGTG1061100.056.1734051
TTGTGTT1197600.051.101623
TTTGTGT1175200.050.697122
TGTGTTT1284750.047.8949434
GTGTTTG1437600.042.8480875
TTCCGAT12600.038.062286
TCCGATC14300.033.8694467
TTGAGGG399900.031.9170829
TTTGAGG594000.031.0162478
GTTTGAA129500.030.1710362
TGTTTGA2112400.030.1705571
GTTTGAG1213400.030.1070082
TTTGAGT416700.027.237323
AGTCCTG32950.027.0923357
CTTGTGT60600.027.0477982
GTTTGAC358900.026.3415767
TGAGTCT22000.026.3309335
TTTGATT291800.025.7182483
TTTGAAC49200.025.3736133
Produced by FastQC (version 0.11.2)