FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l03n02_8033.34200000035d6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l03n02_8033.34200000035d6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17575583
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAA12151.053212E-4150.6865794-95
AGCGCTA9400.009852954129.8469494-95
ACATCCG15152.536046E-4120.8476594-95
GAGTCTA19950.091.7715294-95
TTCTCTA40401.7996426E-675.5297894-95
CGACGTG26650.00239202568.699594-95
CTTTGTG1361000.064.306361
TTTGTGT1479950.059.1729742
ACTTTGT389500.057.412271
TTGTGTT1645200.054.586953
AGTCCGG11401.0597432E-853.53338694-95
TGTGTTT1715250.052.678344
ACATCCA36500.00830918850.1600594-95
GTGTTTG1919150.047.0567975
ATTGGCG14900.040.9584394-95
AGTTCTA60500.001199424340.34913394-95
GATCCCA16657.550043E-736.65349294-95
TTGAGGG333600.035.2401439
TTTGAGG622650.035.1954658
GTTTGAG1448600.034.7902077
Produced by FastQC (version 0.11.2)