##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l03n02_8033.34200000035d6b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17575583 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.38406287859697 33.0 33.0 33.0 33.0 33.0 2 32.383999040031846 33.0 33.0 33.0 33.0 33.0 3 32.36848513076352 33.0 33.0 33.0 33.0 33.0 4 36.21170017518053 37.0 37.0 37.0 37.0 37.0 5 36.18951832209492 37.0 37.0 37.0 37.0 37.0 6 36.173161197554585 37.0 37.0 37.0 37.0 37.0 7 36.14155940090295 37.0 37.0 37.0 37.0 37.0 8 36.17228304745282 37.0 37.0 37.0 37.0 37.0 9 36.156915875848895 37.0 37.0 37.0 37.0 37.0 10-11 36.144126342779074 37.0 37.0 37.0 37.0 37.0 12-13 36.10581620535717 37.0 37.0 37.0 37.0 37.0 14-15 38.081852249225534 40.0 37.0 40.0 37.0 40.0 16-17 38.078453841332035 40.0 37.0 40.0 37.0 40.0 18-19 38.01918670350793 40.0 37.0 40.0 37.0 40.0 20-21 37.97822760701594 40.0 37.0 40.0 37.0 40.0 22-23 37.91703948028353 40.0 37.0 40.0 37.0 40.0 24-25 37.85155015910425 40.0 37.0 40.0 33.0 40.0 26-27 37.796719630865155 40.0 37.0 40.0 33.0 40.0 28-29 37.71969322440115 40.0 37.0 40.0 33.0 40.0 30-31 37.656961080608255 40.0 37.0 40.0 33.0 40.0 32-33 37.600188255490586 40.0 37.0 40.0 33.0 40.0 34-35 37.515789661145234 40.0 37.0 40.0 33.0 40.0 36-37 37.395638369435595 40.0 37.0 40.0 33.0 40.0 38-39 37.27750223136268 40.0 37.0 40.0 33.0 40.0 40-41 37.19054352279523 40.0 37.0 40.0 33.0 40.0 42-43 37.0948348342129 40.0 37.0 40.0 33.0 40.0 44-45 36.93110538637609 40.0 37.0 40.0 33.0 40.0 46-47 36.773661277694174 37.0 37.0 40.0 33.0 40.0 48-49 36.60060545360003 37.0 37.0 40.0 33.0 40.0 50-51 35.29329763911672 37.0 35.0 38.5 30.0 38.5 52-53 35.645560832889586 37.0 35.0 38.5 33.0 40.0 54-55 36.30732422930153 37.0 37.0 40.0 33.0 40.0 56-57 36.25032350278224 37.0 37.0 40.0 33.0 40.0 58-59 36.033676066392786 37.0 37.0 40.0 33.0 40.0 60-61 35.77715783311427 37.0 37.0 40.0 33.0 40.0 62-63 35.51039214460197 37.0 37.0 37.0 33.0 40.0 64-65 35.26380914362841 37.0 33.0 37.0 33.0 40.0 66-67 35.01246857643356 37.0 33.0 37.0 30.0 40.0 68-69 34.74834991248939 37.0 33.0 37.0 27.0 38.5 70-71 34.51802025571499 37.0 33.0 37.0 27.0 37.0 72-73 34.31145174529915 37.0 33.0 37.0 27.0 37.0 74-75 34.003049657015644 37.0 33.0 37.0 27.0 37.0 76-77 33.8752613497942 37.0 33.0 37.0 27.0 37.0 78-79 33.7157858433487 37.0 33.0 37.0 27.0 37.0 80-81 33.56146840193011 37.0 33.0 37.0 27.0 37.0 82-83 33.43878621266788 37.0 33.0 37.0 27.0 37.0 84-85 33.32652148722464 37.0 33.0 37.0 27.0 37.0 86-87 33.22559433732582 37.0 33.0 37.0 27.0 37.0 88-89 33.10638688343937 37.0 33.0 37.0 27.0 37.0 90-91 33.03293759871294 37.0 33.0 37.0 27.0 37.0 92-93 32.92009366061997 37.0 33.0 37.0 27.0 37.0 94-95 32.795103155326345 35.0 33.0 37.0 27.0 37.0 96-97 32.67436713194664 33.0 33.0 37.0 27.0 37.0 98-99 31.23351785826962 33.0 30.0 37.0 21.0 37.0 100-101 2.0197737110626717 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.057219724236411196 2306 2 0.12086507172825378 2306 3 0.12573884423634496 2306 4 0.13768309548424185 2306 5 0.16059477507554476 2306 6 0.13874651533690496 2306 7 0.12290482260441138 2306 8 0.1255680247492279 2306 9 0.13422989326704737 2306 10-11 0.11652456368003783 2306 12-13 0.08980468800831432 2306 14-15 0.10209413806669687 2306 16-17 0.13813279093574948 2306 18-19 0.12101181869500266 2306 20-21 0.14712087262828533 2306 22-23 0.12395325353505626 2306 24-25 0.10567258627775544 2306 26-27 0.0635287224334391 2306 28-29 0.07555716243878408 2306 30-31 0.03233262892759825 2306 32-33 0.008295703849121594 2306 34-35 0.0619371302272782 2306 36-37 -7.329627716003984E-4 2306 38-39 -0.08899398947016124 2306 40-41 -0.005638627140506003 2306 42-43 -0.019070833433382006 2306 44-45 0.009756965739903478 2306 46-47 0.002727928010415326 2306 48-49 -0.08639601777056782 2306 50-51 -0.060527849538246414 2306 52-53 -0.014875925855996286 2306 54-55 -0.01886180955451522 2306 56-57 0.037319096267644625 2306 58-59 0.11338938758795791 2306 60-61 -0.08701623796867608 2306 62-63 -0.04823216714144252 2306 64-65 0.04208677914713377 2306 66-67 -0.026081120319823015 2306 68-69 -0.007348920620174226 2306 70-71 -0.09410187210597343 2306 72-73 -0.010945428914737931 2306 74-75 0.013301646001835365 2306 76-77 0.08946529547918658 2306 78-79 -0.017669081789797758 2306 80-81 0.02656942100502846 2306 82-83 -0.007011768156786502 2306 84-85 -0.12134222277852302 2306 86-87 -0.15164934051671253 2306 88-89 -0.21829683361541186 2306 90-91 -0.16205423426708876 2306 92-93 -0.21811203135498403 2306 94-95 -0.3637267431070441 2306 96-97 -0.2889167883081285 2306 98-99 -0.4657861194428392 2306 100-101 -0.02405260306086321 2307 1 0.051308009128248955 2307 2 -0.011285117242302078 2307 3 -0.01652721061410034 2307 4 -0.017302647333259813 2307 5 0.03713690275804993 2307 6 0.027428372489190167 2307 7 0.028637467139475348 2307 8 0.002162678993101963 2307 9 -0.010176774275187483 2307 10-11 -0.01803148280111344 2307 12-13 -0.04781760392074119 2307 14-15 -0.07441001576975026 2307 16-17 -0.0579437736773869 2307 18-19 -0.09899402237963528 2307 20-21 -0.15545471595943638 2307 22-23 -0.19592433207925097 2307 24-25 -0.17187145139326532 2307 26-27 -0.23301410441471404 2307 28-29 -0.1801032714450841 2307 30-31 -0.2737327265076104 2307 32-33 -0.25537259960815106 2307 34-35 -0.2631740819224433 2307 36-37 -0.2370296794228537 2307 38-39 -0.2473454974457212 2307 40-41 -0.24722262596920785 2307 42-43 -0.2446507242668403 2307 44-45 -0.22921577203474186 2307 46-47 -0.24522228681447444 2307 48-49 -0.24816856723617065 2307 50-51 -0.17727389912944602 2307 52-53 -0.13762091068630866 2307 54-55 -0.04933248238918253 2307 56-57 -0.07544342977623586 2307 58-59 -0.05028189469744859 2307 60-61 -0.05593310640088589 2307 62-63 -0.10612571891799405 2307 64-65 -0.1332936585060267 2307 66-67 -0.11547787029299883 2307 68-69 -0.11028971440136814 2307 70-71 -0.11224987198900038 2307 72-73 -0.10454424437190113 2307 74-75 -0.07779811416901339 2307 76-77 -0.07245768372667527 2307 78-79 -0.12055554464148344 2307 80-81 -0.11395034582371721 2307 82-83 -0.19078999447791745 2307 84-85 -0.12628759610010576 2307 86-87 -0.11747535872638792 2307 88-89 -0.15102386287576053 2307 90-91 -0.1969789304480969 2307 92-93 -0.2865985342139439 2307 94-95 -0.21998780792875294 2307 96-97 -0.11251864380147936 2307 98-99 -0.27364742354106397 2307 100-101 -0.02405260306086321 2201 1 0.11346422028524472 2201 2 0.10735125042672422 2201 3 0.08958076648490731 2201 4 0.12106248845010015 2201 5 0.11432279903593923 2201 6 0.10529460219461129 2201 7 0.07736480436737025 2201 8 0.06952411708537909 2201 9 0.01897470596686901 2201 10-11 0.030186185910864083 2201 12-13 0.019579996301509084 2201 14-15 0.07523397354901107 2201 16-17 0.09603105868858108 2201 18-19 0.033988484585250944 2201 20-21 0.05917626883908156 2201 22-23 0.04766929131773878 2201 24-25 0.02852411860382631 2201 26-27 -0.029921024310674227 2201 28-29 0.03209341340298266 2201 30-31 -0.050800226803858095 2201 32-33 -0.02926989658107715 2201 34-35 0.01264927897261714 2201 36-37 0.03350771913272865 2201 38-39 -0.036481947494110045 2201 40-41 -0.033218333098410824 2201 42-43 -0.013675856483935434 2201 44-45 -0.10914852044252399 2201 46-47 -0.09149854762284093 2201 48-49 -0.12570566013062745 2201 50-51 -0.14094492589899943 2201 52-53 -0.021875038659914026 2201 54-55 0.05746400011408781 2201 56-57 0.008763005171580573 2201 58-59 0.03744218349778805 2201 60-61 -0.03405310191480737 2201 62-63 0.009396163908668598 2201 64-65 -0.02500437790090615 2201 66-67 -0.09399796286764683 2201 68-69 -0.034188848180896514 2201 70-71 -0.04089628982993787 2201 72-73 -0.09763716929396793 2201 74-75 -0.03003399016598962 2201 76-77 -5.48525474421524E-4 2201 78-79 -0.12521418062991074 2201 80-81 -0.13389138141145196 2201 82-83 -0.06761129421213496 2201 84-85 -0.14414275350712558 2201 86-87 -0.2071628134954011 2201 88-89 -0.16620577435076456 2201 90-91 -0.2648759250528414 2201 92-93 -0.3180489405515132 2201 94-95 -0.2978832513181118 2201 96-97 -0.3110717175276463 2201 98-99 -0.5497972783200495 2201 100-101 -0.02405260306086321 1101 1 0.06448921444088995 1101 2 0.01478048924597175 1101 3 0.03542335825765264 1101 4 0.08743386887463345 1101 5 0.06362688764711066 1101 6 0.062046294246144384 1101 7 0.04548507764417309 1101 8 0.058307934106295534 1101 9 0.03850056143262037 1101 10-11 0.03800535904380098 1101 12-13 -0.040709827402821475 1101 14-15 0.06121555552058311 1101 16-17 0.016194372640676136 1101 18-19 -0.01887394144678467 1101 20-21 -0.009606011550268079 1101 22-23 0.0175051668861812 1101 24-25 0.05677237267648394 1101 26-27 0.05232401782722462 1101 28-29 0.022712975753442777 1101 30-31 -0.0726288089942031 1101 32-33 -0.020987363443943252 1101 34-35 -0.02452752126387736 1101 36-37 -0.08905334908855878 1101 38-39 -0.1254065864911098 1101 40-41 -0.05827559370596447 1101 42-43 -0.04446039122422718 1101 44-45 -0.028529360989359986 1101 46-47 -0.03828418611433193 1101 48-49 -0.10272887685329835 1101 50-51 -0.10779424090331702 1101 52-53 0.01551902160322527 1101 54-55 0.1193845740647177 1101 56-57 0.09712086604925929 1101 58-59 0.06283468276812698 1101 60-61 0.06567946329013807 1101 62-63 0.009458903451026401 1101 64-65 0.005462940803511174 1101 66-67 0.021973363962175085 1101 68-69 -0.03534944538713347 1101 70-71 -0.04880943641568791 1101 72-73 -0.0030865660958099284 1101 74-75 -0.029753025517422316 1101 76-77 0.02694988987428104 1101 78-79 -0.009351223804102915 1101 80-81 -0.06083799142887614 1101 82-83 -0.0817450907836701 1101 84-85 -0.15785237417932763 1101 86-87 -0.14647837530991836 1101 88-89 -0.3360228308674209 1101 90-91 -0.2248367784268055 1101 92-93 -0.22855029470807864 1101 94-95 -0.2260176072610136 1101 96-97 -0.27504177382552086 1101 98-99 -0.4382547157339367 1101 100-101 -0.02405260306086321 2304 1 0.056407623336042434 2304 2 0.011902875903246013 2304 3 0.032604471717831984 2304 4 0.05778270608393399 2304 5 0.06642815614605269 2304 6 0.04303447058315868 2304 7 -0.010520325221690996 2304 8 0.04475731160218288 2304 9 0.013107091853100883 2304 10-11 -0.006860548089555607 2304 12-13 0.001953824621303113 2304 14-15 0.036949836312999196 2304 16-17 0.027727729464736228 2304 18-19 0.02953671551038184 2304 20-21 0.020042410656294862 2304 22-23 0.05351546365229609 2304 24-25 0.043873030416676784 2304 26-27 -0.017051912013819503 2304 28-29 0.015339042623701005 2304 30-31 -0.043751665465897815 2304 32-33 -0.03182128404530005 2304 34-35 0.027031283841445486 2304 36-37 0.013338397838076332 2304 38-39 0.0324600645150781 2304 40-41 0.009050770713422196 2304 42-43 -0.018475934251398485 2304 44-45 -0.018529948257388185 2304 46-47 -0.03483163737109152 2304 48-49 -0.07937792547909339 2304 50-51 0.031783121871718834 2304 52-53 0.11111979679701989 2304 54-55 0.11934483559473819 2304 56-57 0.03575233578538928 2304 58-59 0.0039800511809318095 2304 60-61 0.022172690132201467 2304 62-63 -0.026214105710359092 2304 64-65 6.487132567229992E-4 2304 66-67 -0.016467635176496742 2304 68-69 0.020457460884891532 2304 70-71 0.04271765621605539 2304 72-73 0.06943712038136596 2304 74-75 -0.08042897101894653 2304 76-77 0.029256091424663566 2304 78-79 -0.08078944320744341 2304 80-81 8.690009757827966E-4 2304 82-83 -0.1596238786624582 2304 84-85 -0.11408583279650486 2304 86-87 -0.036722874566045505 2304 88-89 0.037067177745839786 2304 90-91 -0.016424555421558296 2304 92-93 -0.10410526299560985 2304 94-95 -0.08118204230916604 2304 96-97 -0.0634171947009392 2304 98-99 -0.3028816047794294 2304 100-101 -0.02405260306086321 2202 1 0.09064911814005683 2202 2 0.03948187860704877 2202 3 0.10689310433472343 2202 4 0.08589227492466023 2202 5 0.12122644341420141 2202 6 0.17173316545134298 2202 7 0.15085319411176812 2202 8 0.10352230670286389 2202 9 0.06525728347730109 2202 10-11 0.06773086138709772 2202 12-13 0.06684661460388241 2202 14-15 0.09275410229472669 2202 16-17 0.07341364842016418 2202 18-19 -0.00993127368212754 2202 20-21 0.025658652039005858 2202 22-23 0.022220734428977096 2202 24-25 0.008268447863663653 2202 26-27 -0.01859490714928569 2202 28-29 0.04839559350411804 2202 30-31 0.016820810612486525 2202 32-33 0.030054052963969013 2202 34-35 -0.016974227166073774 2202 36-37 0.02802369157486595 2202 38-39 -0.017516453548616084 2202 40-41 0.042534803963071965 2202 42-43 -0.06966213441025815 2202 44-45 0.0019373857601081568 2202 46-47 -0.03806951131851122 2202 48-49 -0.05239404007867421 2202 50-51 -0.21776185521648017 2202 52-53 -0.009943041057795199 2202 54-55 0.17493602474705483 2202 56-57 0.09329508643814677 2202 58-59 0.11777474658252629 2202 60-61 0.12115934311557197 2202 62-63 0.08397546019271118 2202 64-65 0.06627379177142956 2202 66-67 0.10117237224495312 2202 68-69 0.009329052380365965 2202 70-71 0.11428226342994208 2202 72-73 0.06949993214514194 2202 74-75 0.009910362287328667 2202 76-77 -0.06181060210610667 2202 78-79 0.0500857034338722 2202 80-81 0.11201857221302447 2202 82-83 0.09732783745156581 2202 84-85 0.04072992097366068 2202 86-87 0.07530458770340687 2202 88-89 0.028892540749524187 2202 90-91 -0.03719652649336069 2202 92-93 -0.07568620126806991 2202 94-95 -0.0043050818573888705 2202 96-97 0.11158799934875674 2202 98-99 -0.009814429047576567 2202 100-101 -0.02405260306086321 1102 1 -0.056983399811002755 1102 2 -0.06763732080270302 1102 3 -0.0755825453548411 1102 4 -0.05334085079916662 1102 5 -0.0699310337143757 1102 6 -0.07300670017004052 1102 7 -0.07636504800519361 1102 8 -0.11229316593895078 1102 9 -0.05956645519889037 1102 10-11 -0.08909905440168586 1102 12-13 -0.04791695898124004 1102 14-15 -0.16959254345805164 1102 16-17 -0.16125628068153475 1102 18-19 -0.19634883428594918 1102 20-21 -0.21400566558294543 1102 22-23 -0.128044137999332 1102 24-25 -0.07614885252752401 1102 26-27 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-0.6555665682154554 1213 76-77 -0.5179621620764294 1213 78-79 -0.4762388064878209 1213 80-81 -0.4872548909037633 1213 82-83 -0.4298928360690084 1213 84-85 -0.42381967876374205 1213 86-87 -0.4911462149271344 1213 88-89 -0.4576980196318843 1213 90-91 -0.4071608319703728 1213 92-93 -0.45768494540278226 1213 94-95 -0.4004859910529106 1213 96-97 -0.45950107770957516 1213 98-99 -0.05300619586737554 1213 100-101 -0.02405260306086321 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 134.0 3 125.0 4 205.0 5 338.0 6 1977.0 7 16489.0 8 30778.0 9 31292.0 10 23818.0 11 18041.0 12 16176.0 13 16570.0 14 17766.0 15 19927.0 16 22664.0 17 26334.0 18 30691.0 19 36024.0 20 41830.0 21 48411.0 22 56645.0 23 66197.0 24 78905.0 25 96190.0 26 121141.0 27 160322.0 28 188153.0 29 222726.0 30 288383.0 31 386331.0 32 535008.0 33 791511.0 34 1352845.0 35 2911490.0 36 6326782.0 37 3578723.0 38 14641.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.218715353123187 23.83757470183843 30.284023169020486 25.659686776017892 2 25.476192887952987 23.521918149191986 30.738975198100952 20.262913764754074 3 21.531295660618056 21.73489673183597 35.78212414354903 20.951683463996943 4 21.25132643533847 22.08724814799809 35.03866294407416 21.62276247258928 5 27.420730350044174 21.365046655714558 30.829449340119453 20.384773654121812 6 27.647343558112127 21.85619475714191 30.688408690881708 19.808052993864255 7 27.761246830482545 25.8505556187797 26.19079323744429 20.197404313293468 8 25.366426820345005 23.320885050801266 30.388370703797023 20.924317425056703 9 22.534728567516684 22.1516597013124 33.82196702515327 21.491644706017645 10-11 26.419693686072332 23.12184712610824 28.742515805308017 21.715943382511412 12-13 25.695847283113448 26.367819599226245 25.519674296625467 22.41665882103484 14-15 25.536581759263566 24.341198998946687 26.88266172725938 23.239557514530368 16-17 24.993105443670675 24.325295643243887 26.613565298621968 24.06803361446347 18-19 24.737395995672912 24.76979677160616 27.069292451436272 23.423514781284656 20-21 23.770660256800234 25.210671056568085 27.457937173170443 23.560731513461235 22-23 23.800500212208135 25.245420102071712 27.07894506992164 23.87513461579851 24-25 24.227274940052435 25.286292155814994 26.721845283322892 23.764587620809685 26-27 23.953369500405604 25.226608348413226 27.031569489900804 23.78845266128037 28-29 24.02242981948543 25.60551727075147 26.49626125073799 23.87579165902511 30-31 24.07150585833005 25.351999911395527 26.59194432177021 23.98454990850421 32-33 24.067294664177393 25.671809066905887 26.305670737403336 23.955225531513385 34-35 23.88237199948278 25.766993941068282 26.425458088912606 23.925175970536333 36-37 24.037539330439017 25.714397499162406 26.46059734827886 23.787465822119717 38-39 24.116712435808164 25.52858039636577 26.38197948316267 23.972727684663397 40-41 23.866216929345658 25.936985974013787 26.19795925596744 23.998837840673115 42-43 23.90306012619373 25.80047356248421 26.218989447864278 24.077476863457782 44-45 23.913297324699993 25.604681597006145 26.376111266961814 24.105909811332037 46-47 23.767843520901604 25.760415943427244 26.44498893119743 24.026751604473727 48-49 23.79442706083151 25.49354097597777 26.38130612433212 24.33072583885861 50-51 23.768320442260485 25.661297671434554 26.683890072833144 23.886491813471817 52-53 24.027011697670538 25.757782478272457 26.44033383251527 23.77487199154173 54-55 23.680831704268144 25.7507196902953 26.286514843230535 24.281933762206027 56-57 23.912585874861705 25.982623813402565 26.32179484711532 23.782995464620406 58-59 23.804015763442095 26.04073239130043 26.336132482485848 23.819119362771627 60-61 24.084764477445265 25.99506872929006 26.005391927407157 23.914774865857517 62-63 23.7664231997001 26.146364646324137 26.280573551431825 23.806638602543938 64-65 23.97748075724385 26.042369230269824 26.11978161881475 23.860368393671582 66-67 24.245416723189866 25.997345431404984 26.119068210658348 23.638169634746806 68-69 24.087626478688566 25.75385657449653 26.4119198569259 23.746597089889004 70-71 24.012863168373897 26.1968823361236 26.157235233212557 23.63301926228995 72-73 23.86792230406193 25.860108540502914 26.391204820539592 23.880764334895563 74-75 23.953685166185675 25.91668940308498 26.35135221974036 23.77827321098898 76-77 23.893927679136517 25.904222122091824 26.206270621313422 23.99557957745823 78-79 24.090741895975075 25.884319610453904 25.97614971082859 24.04878878274243 80-81 24.060213665560006 25.771927395380033 26.268898202702438 23.89896073635752 82-83 23.85094935230557 26.216195096473804 26.22264654071303 23.710209010507597 84-85 23.877171822295946 26.175573117015094 26.145804106369496 23.801450954319467 86-87 23.759731403899544 26.076411323976412 26.31903654728412 23.844820724839927 88-89 23.688560978627496 26.885914464467252 25.874654135284025 23.55087042162122 90-91 23.8399737984957 26.219405046530795 25.957618438594793 23.98300271637871 92-93 23.758771868826464 26.523333254609465 25.92487098590735 23.793023890656716 94-95 23.742717182857316 26.7300763300785 25.537667153675347 23.989539333388837 96-97 23.769972099382628 26.368083436496327 25.705336349607034 24.156608114514018 98-99 24.214596128059004 26.18127882088011 25.56461356571048 24.039511485350403 100-101 31.991530213176393 49.952903765438144 5.8067773365383895 12.248788684847069 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 17070.0 1 10133.5 2 3922.5 3 4181.0 4 4028.0 5 3593.5 6 2727.5 7 2747.0 8 3032.5 9 3270.0 10 3393.5 11 3471.0 12 3681.0 13 4064.5 14 4531.5 15 4958.0 16 5239.5 17 5454.5 18 5748.0 19 6169.5 20 6690.0 21 7306.5 22 8162.5 23 9429.0 24 10946.5 25 12946.5 26 15494.5 27 18589.5 28 22767.0 29 27171.5 30 32002.0 31 39374.5 32 62939.0 33 97715.0 34 133580.5 35 168892.0 36 210721.5 37 281171.0 38 340929.5 39 392062.0 40 447697.0 41 491215.5 42 537869.5 43 626825.0 44 800143.5 45 975651.5 46 1131089.5 47 1231142.5 48 1259403.5 49 1177425.0 50 1044321.0 51 948992.5 52 843805.0 53 762955.5 54 706773.5 55 651881.5 56 530050.5 57 370116.0 58 255269.0 59 183645.5 60 141002.5 61 122112.5 62 99634.0 63 67878.0 64 44551.0 65 29770.5 66 19614.0 67 14098.5 68 10944.5 69 8754.5 70 7442.0 71 6740.0 72 6099.0 73 5530.5 74 4483.5 75 3302.0 76 2756.5 77 2575.5 78 2248.0 79 1753.5 80 1248.5 81 797.0 82 662.0 83 567.0 84 438.0 85 368.5 86 343.0 87 312.0 88 309.0 89 281.0 90 211.5 91 168.5 92 138.5 93 113.5 94 103.0 95 76.0 96 47.0 97 30.5 98 18.0 99 11.5 100 4.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 2.56037025912597E-4 2 0.01693827169204003 3 0.036539328453571066 4 0.012329605225613284 5 0.005046774266321634 6 0.05218603559267423 7 0.05810333574709869 8 0.04836823905073306 9 0.05731246582261311 10-11 0.06344881987698502 12-13 0.07243856434236065 14-15 0.07329486595124611 16-17 0.06259251826809956 18-19 0.07244140919820412 20-21 0.06965629532744376 22-23 0.07190088658794419 24-25 0.07008586855980822 26-27 0.06933198176128781 28-29 0.07182692033601389 30-31 0.08188917545437895 32-33 0.08023346935347749 34-35 0.0936782580697323 36-37 0.09300402723482915 38-39 0.11325086627282863 40-41 0.09609354068084114 42-43 0.09055176149775515 44-45 0.11156386675764896 46-47 0.10571484314346785 48-49 0.10888970226478406 50-51 0.09296135439717704 52-53 0.10159833673796198 54-55 0.10950703598281775 56-57 0.09777769534017734 58-59 0.09589724562764149 60-61 0.1026395539766732 62-63 0.09325721940489826 64-65 0.09318609800881142 66-67 0.09343075561135013 68-69 0.1149577797789126 70-71 0.09628699087819732 72-73 0.09134832113392768 74-75 0.39142087064764797 76-77 0.3829289759548801 78-79 0.40691964528289043 80-81 0.4345005226853641 82-83 0.43028729118118014 84-85 0.41240168249326353 86-87 0.3841152808416085 88-89 0.3715637768602043 90-91 0.3706164398643277 92-93 0.38193612126550797 94-95 0.3928774368395063 96-97 0.35985435020846823 98-99 0.3676919280572371 100-101 99.62246771558019 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7575583E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.50309359698506 #Duplication Level Percentage of deduplicated Percentage of total 1 82.19420741419208 20.962065648141436 2 8.558399985439753 4.365313517382111 3 2.890953609987364 2.2118478150014877 4 1.348446694509866 1.3755824904252094 5 0.7553170025388697 0.9631460105571498 6 0.47218992935373083 0.7225382378317176 7 0.4048343583280771 0.7227169972201438 8 0.3093249713908315 0.6310994957812079 9 0.2600222599716573 0.5968234830021081 >10 1.9429716081397042 10.118304396205277 >50 0.33945823333164304 6.098580941448143 >100 0.3987598454697708 21.635745690753623 >500 0.08809037635583668 15.856057760972972 >1k 0.03681438815307701 13.364228059249808 >5k 1.8702204584402997E-4 0.30979940158457847 >10k+ 2.2300791918591734E-5 0.06615005444297291 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.827654024335921E-5 0.0 0.0 2 7.396625193030582E-5 0.0 0.0 3 8.534567530419901E-5 0.0 0.0 4 9.672509867809222E-5 0.0 0.0 5 9.672509867809222E-5 0.0 0.0 6 1.1948394542587862E-4 0.0 0.0 7 1.3086336879977183E-4 0.0 0.0 8 1.3086336879977183E-4 0.0 0.0 9 1.8207077398229123E-4 0.0 0.0 10-11 2.531921700691238E-4 0.0 0.0 12-13 4.0965924146015524E-4 0.0 0.0 14-15 5.547468894772935E-4 0.0 2.8448558434733005E-6 16-17 7.311279517726382E-4 0.0 5.689711686946601E-6 18-19 9.672509867809222E-4 0.0 5.689711686946601E-6 20-21 0.001285874841249932 0.0 5.689711686946601E-6 22-23 0.0015931192723450483 0.0 5.689711686946601E-6 24-25 0.0019544159644661573 0.0 5.689711686946601E-6 26-27 0.0023726097734567326 0.0 5.689711686946601E-6 28-29 0.003012702338238225 0.0 5.689711686946601E-6 30-31 0.003658484614706664 0.0 5.689711686946601E-6 32-33 0.004409526557383615 0.0 5.689711686946601E-6 34-35 0.005157723644217094 0.0 8.534567530419901E-6 36-37 0.00607661208165897 0.0 1.7069135060839803E-5 38-39 0.007046707924283365 0.0 1.7069135060839803E-5 40-41 0.00843784243174181 0.0 1.7069135060839803E-5 42-43 0.010005358001495598 0.0 1.7069135060839803E-5 44-45 0.011831755453005455 0.0 1.7069135060839803E-5 46-47 0.014221434361523028 0.0 1.7069135060839803E-5 48-49 0.01701508279981381 0.0 1.7069135060839803E-5 50-51 0.020497186352225128 0.0 1.7069135060839803E-5 52-53 0.024710417856409088 0.0 1.7069135060839803E-5 54-55 0.03004167770707805 0.0 1.7069135060839803E-5 56-57 0.03656208730031886 0.0 1.7069135060839803E-5 58-59 0.04466139188668734 0.0 1.7069135060839803E-5 60-61 0.055119081967295196 0.0 1.7069135060839803E-5 62-63 0.06832774764854174 0.0 1.7069135060839803E-5 64-65 0.08434144119145293 0.0 1.7069135060839803E-5 66-67 0.10433793291522676 0.0 1.7069135060839803E-5 68-69 0.12894024624958386 0.0 1.7069135060839803E-5 70-71 0.1592806338202266 0.0 1.7069135060839803E-5 72-73 0.1970261811514304 0.0 1.7069135060839803E-5 74-75 0.24394354372199206 0.0 2.2758846747786404E-5 76-77 0.3011251461758054 0.0 2.2758846747786404E-5 78-79 0.3713873957979089 0.0 2.2758846747786404E-5 80-81 0.45702609125398574 0.0 2.2758846747786404E-5 82-83 0.558644341982852 0.0 2.2758846747786404E-5 84-85 0.6797128721135453 0.0 2.2758846747786404E-5 86-87 0.8240068053503544 0.0 2.2758846747786404E-5 88-89 0.9481477797919989 0.0 2.2758846747786404E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTATAA 1215 1.053212E-4 150.68657 94-95 AGCGCTA 940 0.009852954 129.84694 94-95 ACATCCG 1515 2.536046E-4 120.84765 94-95 GAGTCTA 1995 0.0 91.77152 94-95 TTCTCTA 4040 1.7996426E-6 75.52978 94-95 CGACGTG 2665 0.002392025 68.6995 94-95 CTTTGTG 136100 0.0 64.30636 1 TTTGTGT 147995 0.0 59.172974 2 ACTTTGT 38950 0.0 57.41227 1 TTGTGTT 164520 0.0 54.58695 3 AGTCCGG 1140 1.0597432E-8 53.533386 94-95 TGTGTTT 171525 0.0 52.67834 4 ACATCCA 3650 0.008309188 50.16005 94-95 GTGTTTG 191915 0.0 47.056797 5 ATTGGCG 1490 0.0 40.95843 94-95 AGTTCTA 6050 0.0011994243 40.349133 94-95 GATCCCA 1665 7.550043E-7 36.653492 94-95 TTGAGGG 33360 0.0 35.240143 9 TTTGAGG 62265 0.0 35.195465 8 GTTTGAG 144860 0.0 34.790207 7 >>END_MODULE