##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l03n02_7965.34200000035ca0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14622067 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41565785466583 33.0 33.0 33.0 33.0 33.0 2 32.41790172347042 33.0 33.0 33.0 33.0 33.0 3 32.402163182537734 33.0 33.0 33.0 33.0 33.0 4 36.251337652877666 37.0 37.0 37.0 37.0 37.0 5 36.229773738555565 37.0 37.0 37.0 37.0 37.0 6 36.21783807993767 37.0 37.0 37.0 37.0 37.0 7 36.18460310707098 37.0 37.0 37.0 37.0 37.0 8 36.217572112068694 37.0 37.0 37.0 37.0 37.0 9 36.20145045156748 37.0 37.0 37.0 37.0 37.0 10-11 36.18922040912547 37.0 37.0 37.0 37.0 37.0 12-13 36.15188451126643 37.0 37.0 37.0 37.0 37.0 14-15 38.11744854540743 40.0 37.0 40.0 37.0 40.0 16-17 38.11186024520336 40.0 37.0 40.0 37.0 40.0 18-19 38.058392907104036 40.0 37.0 40.0 37.0 40.0 20-21 38.022734576445316 40.0 37.0 40.0 37.0 40.0 22-23 37.96601308146106 40.0 37.0 40.0 37.0 40.0 24-25 37.9096179767197 40.0 37.0 40.0 35.0 40.0 26-27 37.85441028959859 40.0 37.0 40.0 33.0 40.0 28-29 37.78366098992707 40.0 37.0 40.0 33.0 40.0 30-31 37.71740647201247 40.0 37.0 40.0 33.0 40.0 32-33 37.663685647179705 40.0 37.0 40.0 33.0 40.0 34-35 37.583275264707794 40.0 37.0 40.0 33.0 40.0 36-37 37.47319144413714 40.0 37.0 40.0 33.0 40.0 38-39 37.361349288031576 40.0 37.0 40.0 33.0 40.0 40-41 37.26465813622657 40.0 37.0 40.0 33.0 40.0 42-43 37.16636690284622 40.0 37.0 40.0 33.0 40.0 44-45 36.99616610291828 40.0 37.0 40.0 33.0 40.0 46-47 36.81569763700303 37.0 37.0 40.0 33.0 40.0 48-49 36.61168992044695 37.0 37.0 40.0 33.0 40.0 50-51 35.2851163929149 37.0 35.0 38.5 30.0 38.5 52-53 35.63911367660947 37.0 35.0 38.5 33.0 40.0 54-55 36.24734310819393 37.0 37.0 40.0 33.0 40.0 56-57 36.14000588972817 37.0 37.0 40.0 33.0 40.0 58-59 35.91173402501849 37.0 37.0 40.0 33.0 40.0 60-61 35.65597996507607 37.0 37.0 37.0 33.0 40.0 62-63 35.37278388889888 37.0 33.0 37.0 33.0 40.0 64-65 35.134199186749726 37.0 33.0 37.0 33.0 40.0 66-67 34.90381872822769 37.0 33.0 37.0 33.0 38.5 68-69 34.66591583802755 37.0 33.0 37.0 30.0 37.0 70-71 34.4704480221572 37.0 33.0 37.0 27.0 37.0 72-73 34.28610223164755 37.0 33.0 37.0 27.0 37.0 74-75 34.00379330774507 37.0 33.0 37.0 27.0 37.0 76-77 33.89349617943893 37.0 33.0 37.0 27.0 37.0 78-79 33.74805142118416 37.0 33.0 37.0 27.0 37.0 80-81 33.60943763969895 37.0 33.0 37.0 27.0 37.0 82-83 33.50882659065917 37.0 33.0 37.0 27.0 37.0 84-85 33.40898243045939 37.0 33.0 37.0 27.0 37.0 86-87 33.319527259723266 37.0 33.0 37.0 27.0 37.0 88-89 33.207791244562074 37.0 33.0 37.0 27.0 37.0 90-91 33.1427990310809 37.0 33.0 37.0 27.0 37.0 92-93 33.03873929041633 37.0 33.0 37.0 27.0 37.0 94-95 32.91870930423175 33.0 33.0 37.0 27.0 37.0 96-97 32.80625023808193 33.0 33.0 37.0 27.0 37.0 98-99 31.328734097580046 33.0 30.0 37.0 21.0 37.0 100-101 2.019829583601279 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.08418982520272067 2306 2 0.14839332993206966 2306 3 0.14881081178502598 2306 4 0.1621584937289171 2306 5 0.22143768052113444 2306 6 0.22191832038043913 2306 7 0.20345719132645002 2306 8 0.17730811830444537 2306 9 0.1913034509230016 2306 10-11 0.17535001395025773 2306 12-13 0.11318865543511691 2306 14-15 0.16711579837133428 2306 16-17 0.18279497853190918 2306 18-19 0.13367834384930433 2306 20-21 0.1285560682032738 2306 22-23 0.15512595280760166 2306 24-25 0.1631224493714356 2306 26-27 0.11230028589658758 2306 28-29 0.14403889099551037 2306 30-31 0.1405003113055372 2306 32-33 0.0811867503703283 2306 34-35 0.09370896355810032 2306 36-37 0.009757836026302869 2306 38-39 -0.012931969885180195 2306 40-41 0.04336642845741778 2306 42-43 0.05571913291931452 2306 44-45 0.09089157558432959 2306 46-47 0.06414393374767968 2306 48-49 0.037263928834882165 2306 50-51 0.03530285729578253 2306 52-53 0.07606156287958044 2306 54-55 0.14722984655791294 2306 56-57 0.1897748922209459 2306 58-59 0.14158460692907937 2306 60-61 0.03449037417358625 2306 62-63 0.06748690295431459 2306 64-65 0.15576505750614444 2306 66-67 0.1378354096450849 2306 68-69 0.07519017336954192 2306 70-71 0.017245743709004557 2306 72-73 0.12024332094789969 2306 74-75 0.11608024644480253 2306 76-77 0.1886860831734012 2306 78-79 0.12265553569704934 2306 80-81 0.1854119101832623 2306 82-83 0.10285293473536683 2306 84-85 0.035224709552934996 2306 86-87 0.07251419084813904 2306 88-89 -0.03911572418519427 2306 90-91 0.015258857518894331 2306 92-93 -0.03159162874020893 2306 94-95 -0.16931690986647396 2306 96-97 -0.034741661985137284 2306 98-99 -0.29975955670981946 2306 100-101 -0.024033584940457597 2307 1 0.06526644544045013 2307 2 0.03383079328135352 2307 3 -0.0030875649199160193 2307 4 0.006414073163121259 2307 5 0.06628660696549105 2307 6 0.02586803488364353 2307 7 0.06500483177149619 2307 8 0.04589302083734026 2307 9 -0.02153446877576215 2307 10-11 -0.0011232721563558812 2307 12-13 -0.0022828024139514014 2307 14-15 -0.04544716779147251 2307 16-17 -0.0610313782508527 2307 18-19 -0.05407919575181808 2307 20-21 -0.10560548357475597 2307 22-23 -0.1750145332021944 2307 24-25 -0.1689718216053322 2307 26-27 -0.24200369937334898 2307 28-29 -0.1970354029556347 2307 30-31 -0.2635950602445689 2307 32-33 -0.16358708816766665 2307 34-35 -0.22688401635175381 2307 36-37 -0.2264873697640155 2307 38-39 -0.2014245088128419 2307 40-41 -0.22749136504868517 2307 42-43 -0.1952472139319852 2307 44-45 -0.19333928555230528 2307 46-47 -0.24940767179180057 2307 48-49 -0.24737826731504953 2307 50-51 -0.1278937428018807 2307 52-53 -0.10128378018506368 2307 54-55 -0.06219814079548769 2307 56-57 -0.09754861493983924 2307 58-59 -0.0609188106940195 2307 60-61 -0.01608078786318856 2307 62-63 -0.05614812371573663 2307 64-65 -0.09749344398949233 2307 66-67 -0.08930410339866057 2307 68-69 -0.03603796249419844 2307 70-71 0.0012002816032747887 2307 72-73 -0.035817081080224966 2307 74-75 -0.06732760935604887 2307 76-77 -0.05006226898754562 2307 78-79 -0.061862221202147794 2307 80-81 -0.1201386012360004 2307 82-83 -0.19681298343261489 2307 84-85 -0.15028348663735613 2307 86-87 -0.09110565406567162 2307 88-89 -0.2333808480689683 2307 90-91 -0.19106845299709363 2307 92-93 -0.239942882135459 2307 94-95 -0.21925205996193853 2307 96-97 -0.10232725060492953 2307 98-99 -0.40757220864679766 2307 100-101 -0.024033584940457597 2201 1 0.1556288754986781 2201 2 0.1698911639961196 2201 3 0.1178986134680926 2201 4 0.15904517607125257 2201 5 0.19150982189503196 2201 6 0.13826647781236545 2201 7 0.12872083357731867 2201 8 0.07137173193992652 2201 9 0.029393497700681337 2201 10-11 0.09522733493447078 2201 12-13 0.12176194361905601 2201 14-15 0.13715099700228706 2201 16-17 0.15371184986198472 2201 18-19 0.13648076104467322 2201 20-21 0.15688427100649704 2201 22-23 0.17401577247885314 2201 24-25 0.10058716402258483 2201 26-27 0.093201737441575 2201 28-29 0.1381779492103803 2201 30-31 0.12162930591488674 2201 32-33 0.10930743600202675 2201 34-35 0.1271573520467726 2201 36-37 0.09227934343896038 2201 38-39 0.056577603162175194 2201 40-41 0.041084730197084696 2201 42-43 0.07747891907325766 2201 44-45 0.08261114322255736 2201 46-47 0.06275309815099916 2201 48-49 -0.007455440586632278 2201 50-51 -0.03325038030585148 2201 52-53 0.1300240020162562 2201 54-55 0.19178468056154685 2201 56-57 0.16289288578742145 2201 58-59 0.11802286761962932 2201 60-61 0.1164884003277109 2201 62-63 0.07715558766404484 2201 64-65 0.037312350953385476 2201 66-67 0.00676372353257193 2201 68-69 0.0816250040544304 2201 70-71 0.03938490165709396 2201 72-73 0.0052414384530123925 2201 74-75 0.07363246327457063 2201 76-77 0.013419850053601579 2201 78-79 -0.1416861469095565 2201 80-81 -0.14684738224250538 2201 82-83 4.4472510688109423E-4 2201 84-85 -0.07114439002500461 2201 86-87 -0.08406710699187414 2201 88-89 -0.09804919496869502 2201 90-91 -0.19696539325210694 2201 92-93 -0.23500616691660525 2201 94-95 -0.22836444746501172 2201 96-97 -0.2357528606046344 2201 98-99 -0.4864128493110371 2201 100-101 -0.024033584940457597 1101 1 0.13254882734262452 1101 2 0.06737236729308904 1101 3 0.09339591989891716 1101 4 0.15014268078787296 1101 5 0.12022765706323923 1101 6 0.12406825906837327 1101 7 0.07525948834496177 1101 8 0.1028743284512359 1101 9 0.09879681715609223 1101 10-11 0.10187555256866432 1101 12-13 0.054106468410964226 1101 14-15 0.11313569349076147 1101 16-17 0.06860262533503914 1101 18-19 0.09742033430337926 1101 20-21 0.08014327262794296 1101 22-23 0.0815477898400161 1101 24-25 0.16355159574893463 1101 26-27 0.11994303675621154 1101 28-29 0.08666973376821119 1101 30-31 0.05975599120387898 1101 32-33 0.13015587406625428 1101 34-35 0.14501461189665577 1101 36-37 0.08354466186236209 1101 38-39 8.20023438073747E-4 1101 40-41 0.047657778713222854 1101 42-43 0.07158951465950025 1101 44-45 0.08168716357638317 1101 46-47 0.0363447789688891 1101 48-49 -0.03266697695780607 1101 50-51 -0.09644722281212381 1101 52-53 0.05635507934466233 1101 54-55 0.12191096830066783 1101 56-57 0.1380016840850402 1101 58-59 0.1429104997624222 1101 60-61 0.16845008647179327 1101 62-63 0.125375893722385 1101 64-65 0.14397086326574993 1101 66-67 0.15048602174334036 1101 68-69 0.07070116794212566 1101 70-71 0.06125977426736284 1101 72-73 0.07039533555709454 1101 74-75 0.08972450246599095 1101 76-77 0.1060367943423941 1101 78-79 0.03360059854506403 1101 80-81 0.017694059732910716 1101 82-83 0.06802959135199416 1101 84-85 -0.0347948473819244 1101 86-87 -0.09913489777900963 1101 88-89 -0.25824641802076087 1101 90-91 -0.1588806108352756 1101 92-93 -0.16882462947265964 1101 94-95 -0.12325820510869079 1101 96-97 -0.1230950800075803 1101 98-99 -0.27220093438892334 1101 100-101 -0.024033584940457597 2304 1 0.08229731805241869 2304 2 0.055246205497418543 2304 3 0.10227546259702791 2304 4 0.14624102236817293 2304 5 0.12117430456515166 2304 6 0.10372954437823978 2304 7 0.07647573896234405 2304 8 0.10968289883858517 2304 9 0.08763334216538254 2304 10-11 0.051378932014422674 2304 12-13 0.08782203451411874 2304 14-15 0.09027423910718113 2304 16-17 0.08071378741212243 2304 18-19 0.0909076441875527 2304 20-21 0.07141775590043409 2304 22-23 0.09278923050513299 2304 24-25 0.08444463456628881 2304 26-27 -0.0029199488815621066 2304 28-29 0.05111656152585198 2304 30-31 0.03556246891087511 2304 32-33 0.032311313088079885 2304 34-35 0.07178324934876201 2304 36-37 8.970656110278696E-4 2304 38-39 0.08120922199253044 2304 40-41 0.057375722576701094 2304 42-43 0.061907444359356134 2304 44-45 0.0721653725527247 2304 46-47 0.0020964842886925794 2304 48-49 0.02370807343992709 2304 50-51 0.05775585632706992 2304 52-53 0.15926168986441525 2304 54-55 0.17333091048308802 2304 56-57 0.07813902690540431 2304 58-59 0.07112639992925551 2304 60-61 0.14149994642112063 2304 62-63 0.0729577103146326 2304 64-65 -0.0018914970667083253 2304 66-67 -0.0023690196996781765 2304 68-69 0.07262364082701112 2304 70-71 0.052437004255970976 2304 72-73 0.042426832476181175 2304 74-75 -0.04798975288863261 2304 76-77 0.01888015376316332 2304 78-79 -0.061217292863950945 2304 80-81 0.04904238143471673 2304 82-83 -0.14258527122233033 2304 84-85 -0.10099181904774923 2304 86-87 -0.07238268007361626 2304 88-89 -0.03925476716683107 2304 90-91 -0.09374223317021091 2304 92-93 -0.04836167148467041 2304 94-95 -0.042901293709185495 2304 96-97 -0.008993418304186207 2304 98-99 -0.17418951220591694 2304 100-101 -0.024033584940457597 2202 1 0.09889244362605609 2202 2 0.0644773172984543 2202 3 0.04826197834891843 2202 4 0.08980534454878608 2202 5 0.11290755634470884 2202 6 0.10287627017888923 2202 7 0.15062262463332132 2202 8 0.1167883443170723 2202 9 0.04118765072457364 2202 10-11 0.046329073437000545 2202 12-13 0.02895559571361872 2202 14-15 0.030952187442196077 2202 16-17 0.05284396219501275 2202 18-19 -0.025996191573597116 2202 20-21 0.03568756932110517 2202 22-23 0.06103291664030763 2202 24-25 0.0378512182582611 2202 26-27 -0.02152825765518429 2202 28-29 0.024492003180810684 2202 30-31 -0.001729820591265252 2202 32-33 0.009918586721823885 2202 34-35 -0.0674878213671235 2202 36-37 -0.04962069874638786 2202 38-39 -0.08392506123524157 2202 40-41 0.028759683574982375 2202 42-43 -0.07949369643313986 2202 44-45 -0.07119713120071225 2202 46-47 -0.03328291868358946 2202 48-49 -0.04931319043796378 2202 50-51 -0.23517464383701991 2202 52-53 -0.01747192932409547 2202 54-55 0.11979293212149145 2202 56-57 0.08671885744472974 2202 58-59 0.08599876754133362 2202 60-61 0.06087438308318838 2202 62-63 0.06065770311636953 2202 64-65 0.07433943593721892 2202 66-67 0.07841983390217422 2202 68-69 -0.025367055024453578 2202 70-71 0.05754281744792422 2202 72-73 0.06459400699173301 2202 74-75 -0.0029051903867269857 2202 76-77 -0.11027385416017665 2202 78-79 3.0157620328452595E-4 2202 80-81 0.029537311390313903 2202 82-83 0.0370996069116174 2202 84-85 0.0154869080443234 2202 86-87 0.08366836847778814 2202 88-89 -0.015944642788653596 2202 90-91 -0.06948140153389915 2202 92-93 -0.13348865033674429 2202 94-95 -0.14452714214335316 2202 96-97 -0.02479303967034241 2202 98-99 -0.08952118551136223 2202 100-101 -0.024033584940457597 1102 1 -0.07228688893796686 1102 2 -0.10277097871026797 1102 3 -0.09168772763459998 1102 4 -0.10129283853954973 1102 5 -0.08711177172921936 1102 6 -0.11914769400635805 1102 7 -0.1384521037086941 1102 8 -0.13661402453922022 1102 9 -0.08064592287024652 1102 10-11 -0.11449927187529596 1102 12-13 -0.06410414757018401 1102 14-15 -0.22746873822092795 1102 16-17 -0.20405986866092718 1102 18-19 -0.2522325287150622 1102 20-21 -0.232372170978131 1102 22-23 -0.14576741910317992 1102 24-25 -0.11872412596329696 1102 26-27 -0.11858543874551941 1102 28-29 -0.07536332061282991 1102 30-31 -0.1565203513826745 1102 32-33 -0.10909699678061457 1102 34-35 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-0.5132837386660754 1213 80-81 -0.5567556626290227 1213 82-83 -0.460368955721016 1213 84-85 -0.4455297703555985 1213 86-87 -0.5392716622754747 1213 88-89 -0.4889658636492271 1213 90-91 -0.45071687062388577 1213 92-93 -0.49922103906099835 1213 94-95 -0.43563662907575207 1213 96-97 -0.5188879878865293 1213 98-99 -0.15234588506487867 1213 100-101 -0.024033584940457597 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 79.0 3 69.0 4 174.0 5 196.0 6 1178.0 7 8617.0 8 16443.0 9 16779.0 10 13348.0 11 11154.0 12 10993.0 13 11784.0 14 13441.0 15 15207.0 16 18099.0 17 21019.0 18 24569.0 19 29232.0 20 34585.0 21 40429.0 22 47829.0 23 56446.0 24 67253.0 25 80733.0 26 100849.0 27 133027.0 28 151296.0 29 181142.0 30 237029.0 31 315188.0 32 443759.0 33 665239.0 34 1162040.0 35 2633601.0 36 5656233.0 37 2395254.0 38 7754.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.311693024376773 23.639036812525536 29.440370607810102 25.60889955528759 2 26.679530592122475 23.43508534206767 28.53189481723114 21.353489248578718 3 22.46324762619718 21.66088445145214 33.84363369450543 22.032234227845255 4 22.529831159505093 21.682034383952196 32.941758575554225 22.846375880988486 5 27.52089641721186 21.05941795200817 29.997142545662157 21.422543085117805 6 28.79940256896985 21.587950493801596 28.801954815788676 20.81069212143987 7 27.64580661906449 25.472169819164233 25.53772448796022 21.34429907381107 8 26.84030114591011 22.75223693181056 28.359147260492364 22.048314661786968 9 23.49465301385536 21.91044927274419 31.870925095662745 22.723972617737704 10-11 27.130936793357012 22.646925369604425 27.438682289087783 22.783455547950776 12-13 26.462813304784845 25.404009497776446 24.867389029581606 23.265788167857103 14-15 26.397300698638798 23.767653450954967 25.58979205706306 24.245253793343174 16-17 26.148667952961173 23.637923265084908 25.425744891461633 24.78766389049229 18-19 25.70918744090126 23.98287889005428 25.745829386573522 24.56210428247094 20-21 24.87976648205562 24.50043738077422 26.135150590795963 24.4846455463742 22-23 25.044630376304994 24.515882322949064 25.913157859617147 24.526329441128794 24-25 25.298982799091785 24.562144670383393 25.568772049145217 24.570100481379605 26-27 25.02489642358936 24.533431800087303 25.768813844830753 24.672857931492583 28-29 25.17423957290683 24.76337038387679 25.351350986963773 24.711039056252602 30-31 25.18996213231503 24.74572840802577 25.24832883185938 24.81598062779982 32-33 24.943966548917764 24.896545073262192 25.1798282450755 24.97966013274454 34-35 24.732728907191625 25.00477714445698 25.24233889950952 25.020155048841875 36-37 24.94807105031005 24.90640399146739 25.19456178825406 24.950963169968503 38-39 25.127435885638665 24.911326176712556 25.04943592178875 24.911802015860026 40-41 24.779600082131534 25.008898210394392 25.06814887685743 25.14335283061665 42-43 24.944489888117296 24.932863533220846 25.207450176431735 24.915196402230126 44-45 25.000395383698482 24.90500350368152 25.17409377542822 24.92050733719178 46-47 24.826216804136315 25.071432745085954 25.0214016690649 25.080948781712838 48-49 25.051975443395875 24.617211051827425 25.26036672785667 25.070446776920036 50-51 24.82802793719672 25.080535082684147 25.3680419309171 24.723395049202033 52-53 25.096109907961633 24.821449536693322 25.404958208097966 24.677482347247082 54-55 24.832401867459453 24.849175373371395 25.153809626048034 25.164613133121115 56-57 24.824121418293753 24.83770648948822 25.392924823727796 24.945247268490224 58-59 24.68340598761454 24.91566374916627 25.308932292334273 25.091997970884915 60-61 24.936841722615103 24.837696422533618 25.02530404387545 25.20015781097583 62-63 24.556030778511857 25.149139740271863 25.254029303157978 25.040800178058298 64-65 24.852082812883815 25.108500783313964 25.083437063864157 24.955979339938068 66-67 24.928370655524915 25.035214431114312 25.15574524449023 24.880669668870546 68-69 24.998676876365618 25.221365601893904 25.142234934859527 24.637722586880955 70-71 24.93699883452935 25.256930812745924 25.092725783968202 24.713344568756522 72-73 25.187145064394606 24.998654937631546 25.031442971956714 24.782757026017137 74-75 25.397819269373915 24.940112517717026 25.091223646220907 24.57084456668815 76-77 25.361984670007192 24.963956252606334 24.899995184212788 24.77406389317369 78-79 25.452597129201195 24.807864430140324 25.055428657497437 24.684109783161052 80-81 25.394669407436698 24.83674705252115 24.959252659133398 24.80933088090876 82-83 25.007812717854634 25.20705591103708 24.979638855314235 24.805492515794054 84-85 24.908541874888904 25.18284672120557 25.1045381640652 24.804073239840328 86-87 25.031366379634655 25.20927052606547 25.05277158245822 24.70659151184165 88-89 25.029992234195014 25.48682488471845 24.74204962543985 24.741133255646684 90-91 24.839126893055056 25.261083852044962 25.03296570917588 24.866823545724102 92-93 24.783823499074973 25.480885257449298 24.779007773021206 24.956283470454522 94-95 24.965988251095293 25.38650120364053 25.009235205520014 24.63827533974417 96-97 24.954363803243115 25.321155490588517 24.75233050537734 24.97215020079103 98-99 25.134979027286146 25.12924765236706 24.8865084807189 24.849264839627892 100-101 31.800515227947972 50.14586157063739 5.90088594073981 12.152737260674822 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 10944.0 1 6215.0 2 1962.5 3 2132.0 4 2134.5 5 1965.5 6 1483.0 7 1567.0 8 1743.0 9 1793.0 10 1840.0 11 1984.5 12 2074.5 13 2205.5 14 2460.5 15 2758.0 16 2902.5 17 2904.0 18 2851.5 19 2724.0 20 2671.5 21 2778.0 22 2968.5 23 3279.0 24 3663.5 25 4154.0 26 4772.0 27 5807.5 28 7619.0 29 9830.5 30 12220.0 31 16176.0 32 29824.0 33 45105.0 34 58325.5 35 88363.0 36 131751.0 37 188007.5 38 214151.5 39 212471.5 40 232056.0 41 273906.0 42 331276.5 43 390162.0 44 428839.0 45 511195.0 46 650410.5 47 750806.5 48 843625.0 49 937885.5 50 1021385.0 51 1060926.0 52 1001684.5 53 925004.0 54 897792.0 55 836689.5 56 714553.0 57 551485.5 58 380308.5 59 259199.0 60 178498.0 61 124138.5 62 87050.0 63 53003.5 64 26285.0 65 15404.5 66 8581.0 67 5732.0 68 4720.5 69 3915.5 70 3046.5 71 2574.0 72 2391.5 73 2187.5 74 1797.5 75 1431.5 76 1193.0 77 1056.5 78 1206.0 79 1159.0 80 728.5 81 400.5 82 285.5 83 205.5 84 147.0 85 109.5 86 88.5 87 81.5 88 72.5 89 57.0 90 49.5 91 45.5 92 36.0 93 29.5 94 22.0 95 11.0 96 6.5 97 8.0 98 7.0 99 3.5 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 2.2568628635062334E-4 2 0.016222056703747836 3 0.03501556927621793 4 0.011626263236244232 5 0.004520564705386728 6 0.05124446495834002 7 0.05683191029011151 8 0.04759245050648448 9 0.056264275085047825 10-11 0.06278524096490598 12-13 0.07137499780297821 14-15 0.07237348864562035 16-17 0.06176965267632818 18-19 0.071108277646382 20-21 0.06897109690442535 22-23 0.07100227348158096 24-25 0.06911471545028483 26-27 0.06858469462627959 28-29 0.07085523544653434 30-31 0.08105899118093222 32-33 0.07931847118468272 34-35 0.09194664475275623 36-37 0.09167308561778577 38-39 0.11141379669509105 40-41 0.09432660922699916 42-43 0.08902298149775952 44-45 0.10943733194492954 46-47 0.10357974696737471 48-49 0.10703001155718955 50-51 0.09144739933143516 52-53 0.09965759286973586 54-55 0.10736512149752836 56-57 0.09656637464456974 58-59 0.09388891461104644 60-61 0.10065608371237801 62-63 0.09142688239631237 64-65 0.09096867084523687 66-67 0.09133113669907271 68-69 0.11269268565107793 70-71 0.09441551594586456 72-73 0.08944699815696372 74-75 0.3872742478884825 76-77 0.37883494857464406 78-79 0.40334584706799664 80-81 0.4318233530184208 82-83 0.42725149597522705 84-85 0.4089811652483879 86-87 0.3796658844471168 88-89 0.3673386259275108 90-91 0.3660187031012784 92-93 0.37756973757540574 94-95 0.38899767043879635 96-97 0.3552438926726297 98-99 0.3634027938731234 100-101 99.61904496812933 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4622067E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.671675483571073 #Duplication Level Percentage of deduplicated Percentage of total 1 75.32930845402575 9.545485511312412 2 11.182065036167483 2.8339099874900153 3 3.867690654188549 1.47030362522154 4 1.8370535709628741 0.9311418678870778 5 1.3364726094425121 0.8467673599768469 6 0.6823792838189316 0.5188133304759086 7 0.5970268697741019 0.5295731524124767 8 0.397151691403953 0.4026061880977805 9 0.30667269928249474 0.3497451232480698 >10 2.6967967929487373 7.087393238323464 >50 0.5348621988137814 4.849061458183935 >100 0.8608046637349623 25.18237322217285 >500 0.24519446498969638 22.21280446698993 >1k 0.12613554861675882 22.922546773133202 >5k 3.854618293181408E-4 0.3174746950746432 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.0942365398818102E-4 0.0 0.0 2 1.0942365398818102E-4 0.0 0.0 3 1.0942365398818102E-4 0.0 0.0 4 1.0942365398818102E-4 0.0 0.0 5 1.1626263236244232E-4 0.0 0.0 6 1.1626263236244232E-4 0.0 0.0 7 1.2310161073670364E-4 0.0 0.0 8 1.4361854585948758E-4 0.0 0.0 9 1.6413548098227152E-4 0.0 0.0 10-11 2.222667971634927E-4 0.0 0.0 12-13 3.2143198359028177E-4 0.0 0.0 14-15 4.4795308351411605E-4 0.0 0.0 16-17 7.146732401103073E-4 0.0 0.0 18-19 0.001015588288577805 0.0 0.0 20-21 0.0013165033370453028 0.0 0.0 22-23 0.001672130212506891 0.0 0.0 24-25 0.002072210447401178 0.0 0.0 26-27 0.0024996465957925103 0.0 0.0 28-29 0.003015989463049239 0.0 0.0 30-31 0.003498137438434662 0.0 0.0 32-33 0.004127323448866703 0.0 0.0 34-35 0.004941161875403799 0.0 0.0 36-37 0.005693449496572543 0.0 0.0 38-39 0.00666458442571765 0.0 0.0 40-41 0.0077895963682836356 0.0 0.0 42-43 0.009239459783627033 0.0 0.0 44-45 0.010870556125888359 0.0 0.0 46-47 0.01291541065979249 0.0 0.0 48-49 0.015456091125830568 0.0 0.0 50-51 0.018663571983359124 0.0 0.0 52-53 0.022674632799863384 0.0 0.0 54-55 0.027373010942980905 0.0 0.0 56-57 0.03306987992874058 0.0 0.0 58-59 0.04000118451105442 0.0 0.0 60-61 0.04897734362727239 0.0 0.0 62-63 0.059878675155844927 0.0 0.0 64-65 0.07360450475298738 0.0 0.0 66-67 0.09039761615098604 0.0 0.0 68-69 0.11141037720590392 0.0 0.0 70-71 0.13744636787671674 0.0 0.0 72-73 0.1699109982193352 0.0 0.0 74-75 0.21003870383031345 0.0 0.0 76-77 0.2592177973196266 0.0 0.0 78-79 0.31997528119656404 0.0 0.0 80-81 0.3968112032313899 0.0 0.0 82-83 0.48978027525109824 0.0 0.0 84-85 0.6029927232586201 0.0 0.0 86-87 0.7410033068512133 0.0 0.0 88-89 0.8621831646647495 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGACG 265 2.2436693E-4 459.3387 94-95 GGCGTTA 380 6.6062884E-4 320.32834 94-95 GGCACTA 435 9.903315E-4 279.82706 94-95 ACGTGCG 555 0.0020537644 219.32393 94-95 GCGTCGA 600 0.0025934933 202.87462 94-95 GGGCCCA 635 0.0030730208 191.69257 94-95 GGGACCC 735 0.00475962 165.61194 94-95 ATTCCCG 765 1.0397762E-6 159.11734 94-95 GTATTTG 1185 9.633117E-5 154.08199 94-95 AGTGTCG 395 4.965841E-10 154.08199 94-95 GATCCGC 405 1.1141401E-6 150.2775 94-95 AGCGCAT 410 8.317785E-4 148.44485 94-95 CCTGGCA 880 0.008154246 138.3236 94-95 ATTATGA 920 0.009312916 132.30952 94-95 TATTATA 925 0.009465001 131.59435 94-95 AGCACAA 935 0.009774103 130.18692 94-95 GTGTGCA 1535 2.6998148E-4 118.94929 94-95 GAGATCC 585 1.469889E-6 104.03826 94-95 AGCCCGG 610 2.6961972E-5 99.77441 94-95 AGATCGA 2030 8.204775E-4 89.944405 94-95 >>END_MODULE