FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l03n02_7965.34200000035ca0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l03n02_7965.34200000035ca0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14622067
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG2652.2436693E-4459.338794-95
GGCGTTA3806.6062884E-4320.3283494-95
GGCACTA4359.903315E-4279.8270694-95
ACGTGCG5550.0020537644219.3239394-95
GCGTCGA6000.0025934933202.8746294-95
GGGCCCA6350.0030730208191.6925794-95
GGGACCC7350.00475962165.6119494-95
ATTCCCG7651.0397762E-6159.1173494-95
GTATTTG11859.633117E-5154.0819994-95
AGTGTCG3954.965841E-10154.0819994-95
GATCCGC4051.1141401E-6150.277594-95
AGCGCAT4108.317785E-4148.4448594-95
CCTGGCA8800.008154246138.323694-95
ATTATGA9200.009312916132.3095294-95
TATTATA9250.009465001131.5943594-95
AGCACAA9350.009774103130.1869294-95
GTGTGCA15352.6998148E-4118.9492994-95
GAGATCC5851.469889E-6104.0382694-95
AGCCCGG6102.6961972E-599.7744194-95
AGATCGA20308.204775E-489.94440594-95
Produced by FastQC (version 0.11.2)