##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l03n02_7765.34200000035c6c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14427885 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.397180251991195 33.0 33.0 33.0 33.0 33.0 2 32.39860284442245 33.0 33.0 33.0 33.0 33.0 3 32.38230267291429 33.0 33.0 33.0 33.0 33.0 4 36.23046482557908 37.0 37.0 37.0 37.0 37.0 5 36.20749028703791 37.0 37.0 37.0 37.0 37.0 6 36.18788082938005 37.0 37.0 37.0 37.0 37.0 7 36.15298735746785 37.0 37.0 37.0 37.0 37.0 8 36.18601125528794 37.0 37.0 37.0 37.0 37.0 9 36.16825764829703 37.0 37.0 37.0 37.0 37.0 10-11 36.16035264350943 37.0 37.0 37.0 37.0 37.0 12-13 36.11983873589234 37.0 37.0 37.0 37.0 37.0 14-15 38.06940455929612 40.0 37.0 40.0 37.0 40.0 16-17 38.069221649604216 40.0 37.0 40.0 37.0 40.0 18-19 37.99986342419558 40.0 37.0 40.0 37.0 40.0 20-21 37.95967191310438 40.0 37.0 40.0 37.0 40.0 22-23 37.90656000515668 40.0 37.0 40.0 35.0 40.0 24-25 37.84294004284065 40.0 37.0 40.0 33.0 40.0 26-27 37.79218246472023 40.0 37.0 40.0 33.0 40.0 28-29 37.71563066936007 40.0 37.0 40.0 33.0 40.0 30-31 37.64947159614871 40.0 37.0 40.0 33.0 40.0 32-33 37.59487215208605 40.0 37.0 40.0 33.0 40.0 34-35 37.51489566904644 40.0 37.0 40.0 33.0 40.0 36-37 37.39501895114911 40.0 37.0 40.0 33.0 40.0 38-39 37.266445567039106 40.0 37.0 40.0 33.0 40.0 40-41 37.17025305510822 40.0 37.0 40.0 33.0 40.0 42-43 37.06430166306427 40.0 37.0 40.0 33.0 40.0 44-45 36.878094329141106 38.5 37.0 40.0 33.0 40.0 46-47 36.700190464506754 37.0 37.0 40.0 33.0 40.0 48-49 36.50752962059235 37.0 37.0 40.0 33.0 40.0 50-51 35.17755027850582 37.0 35.0 38.5 30.0 38.5 52-53 35.53696335256346 37.0 35.0 38.5 33.0 40.0 54-55 36.18289783984278 37.0 37.0 40.0 33.0 40.0 56-57 36.104392466394074 37.0 37.0 40.0 33.0 40.0 58-59 35.87832326082444 37.0 37.0 40.0 33.0 40.0 60-61 35.61637242742093 37.0 37.0 37.0 33.0 40.0 62-63 35.350549092954374 37.0 33.0 37.0 33.0 40.0 64-65 35.09173125513546 37.0 33.0 37.0 33.0 40.0 66-67 34.846776017413504 37.0 33.0 37.0 27.0 38.5 68-69 34.59649553624804 37.0 33.0 37.0 27.0 37.0 70-71 34.38052760331816 37.0 33.0 37.0 27.0 37.0 72-73 34.19511508443545 37.0 33.0 37.0 27.0 37.0 74-75 33.89350947834697 37.0 33.0 37.0 27.0 37.0 76-77 33.78021414781169 37.0 33.0 37.0 27.0 37.0 78-79 33.62927047172887 37.0 33.0 37.0 27.0 37.0 80-81 33.48739014762039 37.0 33.0 37.0 27.0 37.0 82-83 33.366028700672345 37.0 33.0 37.0 27.0 37.0 84-85 33.26153573444756 37.0 33.0 37.0 27.0 37.0 86-87 33.16749953302234 37.0 33.0 37.0 27.0 37.0 88-89 33.050220735748866 37.0 33.0 37.0 27.0 37.0 90-91 32.99300846936332 37.0 33.0 37.0 27.0 37.0 92-93 32.886034439559225 35.0 33.0 37.0 27.0 37.0 94-95 32.7638114664762 33.0 33.0 37.0 27.0 37.0 96-97 32.64353465528731 33.0 33.0 37.0 27.0 37.0 98-99 31.175812047295913 33.0 30.0 37.0 18.5 37.0 100-101 2.020010278706824 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.043643463289477324 2306 2 0.09383382016157071 2306 3 0.10036649261714103 2306 4 0.09989653295667722 2306 5 0.14070079660933743 2306 6 0.0809969141603375 2306 7 0.06639301800484532 2306 8 0.023381825589510186 2306 9 0.048159962155089886 2306 10-11 0.07817662103167322 2306 12-13 0.032869695917590036 2306 14-15 0.05140424687798628 2306 16-17 0.0920060776778513 2306 18-19 0.07777325845763272 2306 20-21 0.04754875441584261 2306 22-23 -0.0018221731072003422 2306 24-25 0.06705662327574657 2306 26-27 0.004138990895867778 2306 28-29 0.052062537141601695 2306 30-31 8.444933787714604E-4 2306 32-33 -0.05854879237062249 2306 34-35 0.025624185426813995 2306 36-37 -0.04374636647579422 2306 38-39 -0.09998320927322624 2306 40-41 -0.027153985005703873 2306 42-43 -0.036807135500048105 2306 44-45 -0.08501486727730878 2306 46-47 -0.0698520074066451 2306 48-49 -0.1552892704961124 2306 50-51 -0.1552057101749753 2306 52-53 -0.04097953799347209 2306 54-55 -0.0413340814301435 2306 56-57 0.05601139683787437 2306 58-59 -0.014350441162193306 2306 60-61 -0.1444766595467044 2306 62-63 -0.08069196439315363 2306 64-65 0.029079271742631363 2306 66-67 -0.04288003777333671 2306 68-69 -0.047417778778736874 2306 70-71 -0.12185466912985277 2306 72-73 -0.04471047441038678 2306 74-75 0.0016255838901813036 2306 76-77 0.03864502266424097 2306 78-79 -0.003953649194109232 2306 80-81 0.010719577820907489 2306 82-83 -0.01965559655384652 2306 84-85 -0.11930788969000616 2306 86-87 -0.16727094341891302 2306 88-89 -0.26482948702730624 2306 90-91 -0.1579753209540229 2306 92-93 -0.1670276586838284 2306 94-95 -0.4160204932040301 2306 96-97 -0.29647107544023044 2306 98-99 -0.4774251413197099 2306 100-101 -0.024122349237905905 2307 1 0.07153184842036353 2307 2 0.056230419708725776 2307 3 0.011107505462909728 2307 4 4.1585033656588166E-5 2307 5 0.06713209427029199 2307 6 0.05397637373071262 2307 7 0.08276720838848917 2307 8 0.038425990962934975 2307 9 0.01629761940932184 2307 10-11 -0.008455639422905392 2307 12-13 -0.007346323636774343 2307 14-15 -0.0244215506499188 2307 16-17 -0.04822080033424214 2307 18-19 -0.05283398699017283 2307 20-21 -0.11505008798828698 2307 22-23 -0.1954870389617085 2307 24-25 -0.17018785329053543 2307 26-27 -0.2603013078558618 2307 28-29 -0.21837469592681913 2307 30-31 -0.26388212858177695 2307 32-33 -0.20024365362998964 2307 34-35 -0.22868616978967538 2307 36-37 -0.23980662396539998 2307 38-39 -0.15776486787800081 2307 40-41 -0.2100071709600897 2307 42-43 -0.20407499032736354 2307 44-45 -0.2097990140285475 2307 46-47 -0.20889071388210567 2307 48-49 -0.26279090979979003 2307 50-51 -0.10569515869454449 2307 52-53 -0.09600193855791872 2307 54-55 -0.06322422605724398 2307 56-57 -0.08223341223077085 2307 58-59 -0.09200357499970835 2307 60-61 -0.026047556186767906 2307 62-63 -0.005972242987937193 2307 64-65 -0.07789755247087271 2307 66-67 -0.09927588240655183 2307 68-69 -0.10310199635486583 2307 70-71 -0.10219728418203289 2307 72-73 -0.06381229444681935 2307 74-75 -0.032930035010267034 2307 76-77 -0.07941676222456096 2307 78-79 -0.1303613631805547 2307 80-81 -0.07275794923788226 2307 82-83 -0.18947721431414521 2307 84-85 -0.17186675141761043 2307 86-87 -0.11424075029775338 2307 88-89 -0.17158437839086815 2307 90-91 -0.22188405831473546 2307 92-93 -0.3130532687832144 2307 94-95 -0.3279660865618368 2307 96-97 -0.2208790854024656 2307 98-99 -0.4939535317549577 2307 100-101 -0.024122349237905905 2201 1 0.1512087585091706 2201 2 0.1273534524232005 2201 3 0.1067965880545998 2201 4 0.1084256371289527 2201 5 0.12526963817964543 2201 6 0.13622335619116654 2201 7 0.12784824734040257 2201 8 0.11063191460264221 2201 9 0.03081207070930958 2201 10-11 0.07762937718949559 2201 12-13 0.08558940385240277 2201 14-15 0.1093685335704393 2201 16-17 0.1348371138976674 2201 18-19 0.12606131947163846 2201 20-21 0.13633876843330484 2201 22-23 0.13840770722118378 2201 24-25 0.11545990734993694 2201 26-27 0.08526397984430645 2201 28-29 0.11702565810433185 2201 30-31 0.05157823460688604 2201 32-33 0.043798272910727576 2201 34-35 0.11982384591557604 2201 36-37 0.08053768065840217 2201 38-39 0.03628492204794043 2201 40-41 0.05868457834990437 2201 42-43 0.07480651559536966 2201 44-45 -0.010270324317119162 2201 46-47 0.0022499017629584728 2201 48-49 -0.08896806103822996 2201 50-51 -0.10716332496648562 2201 52-53 0.0404194576536554 2201 54-55 0.13540037061171262 2201 56-57 0.07351829715781122 2201 58-59 0.08011971985668254 2201 60-61 0.017815501236960074 2201 62-63 0.04705464568991147 2201 64-65 6.922798652908568E-4 2201 66-67 -0.027083340623178742 2201 68-69 0.00965802452577691 2201 70-71 0.05012606775476769 2201 72-73 -0.015409494959570225 2201 74-75 0.025041722163976488 2201 76-77 0.05662564615020926 2201 78-79 -0.12851815602889616 2201 80-81 -0.11698715053309883 2201 82-83 2.374429312297366E-4 2201 84-85 -0.10795590688334755 2201 86-87 -0.13572163900070677 2201 88-89 -0.13224888779471655 2201 90-91 -0.17474838469608045 2201 92-93 -0.20904371717615078 2201 94-95 -0.20792506379433462 2201 96-97 -0.21074209299131752 2201 98-99 -0.5643922217152699 2201 100-101 -0.024122349237905905 1101 1 0.05733277289692751 1101 2 0.018629713803953507 1101 3 0.049834115544094004 1101 4 0.10475669953816436 1101 5 0.09835723002923658 1101 6 0.10266759431894457 1101 7 0.0868375500116727 1101 8 0.04853726462887664 1101 9 0.04705862618020262 1101 10-11 0.01872466379211346 1101 12-13 -0.07080513438916824 1101 14-15 0.023720216872774813 1101 16-17 0.0224109710638416 1101 18-19 -0.009028601402668812 1101 20-21 0.005478548629099578 1101 22-23 0.03781179039209093 1101 24-25 0.05116898348474308 1101 26-27 0.06599788984847521 1101 28-29 0.040256868533070644 1101 30-31 0.035085330331433795 1101 32-33 0.0676819668674753 1101 34-35 0.05159371832828441 1101 36-37 -0.057310874787965815 1101 38-39 -0.055498814614793446 1101 40-41 -0.06876862453315624 1101 42-43 0.018191235993036514 1101 44-45 0.03857712096920807 1101 46-47 0.018028928262033617 1101 48-49 -0.08720418447180123 1101 50-51 -0.1571225546225179 1101 52-53 -0.05003859513291076 1101 54-55 0.0745913524020736 1101 56-57 0.0944162487576321 1101 58-59 0.07995039617046018 1101 60-61 0.07515890456628682 1101 62-63 0.044395542871328075 1101 64-65 0.0669448958843617 1101 66-67 0.0702318263571371 1101 68-69 -0.05553385652622467 1101 70-71 -0.02237629189823309 1101 72-73 0.0023104838138650052 1101 74-75 0.010512337837539576 1101 76-77 0.026463296163690586 1101 78-79 -0.033925634029159824 1101 80-81 -0.0723933948564266 1101 82-83 -0.08938825772131054 1101 84-85 -0.11132758191035208 1101 86-87 -0.08318655767759253 1101 88-89 -0.34739869429123615 1101 90-91 -0.2755835392232413 1101 92-93 -0.3231918600603265 1101 94-95 -0.29182929769221033 1101 96-97 -0.33555637679729244 1101 98-99 -0.465353464265462 1101 100-101 -0.024122349237905905 2304 1 0.0661939543417347 2304 2 0.06015891454990907 2304 3 0.04309098226143959 2304 4 0.05167634605207638 2304 5 0.08051970216150295 2304 6 0.08423860927703686 2304 7 0.03194889612895224 2304 8 0.118571296918482 2304 9 0.0455135744501618 2304 10-11 0.0220093279422926 2304 12-13 0.020941940994283925 2304 14-15 0.04356360159402328 2304 16-17 0.05771034059667102 2304 18-19 0.08651654121684516 2304 20-21 0.06188538073003258 2304 22-23 0.07531153643061828 2304 24-25 0.0624002300003923 2304 26-27 5.563238613532917E-4 2304 28-29 0.03237969704251498 2304 30-31 -0.04211599036167257 2304 32-33 0.04071552628681019 2304 34-35 0.04205503901406615 2304 36-37 0.023279494692424407 2304 38-39 0.06458754226169106 2304 40-41 0.09625639981084078 2304 42-43 0.09862704095330344 2304 44-45 0.08449765494983552 2304 46-47 0.029452683572706917 2304 48-49 0.023366909948293824 2304 50-51 0.11135382603863775 2304 52-53 0.15698021056744693 2304 54-55 0.21397046006712372 2304 56-57 0.10942153955327427 2304 58-59 0.10624122662517266 2304 60-61 0.11907060802393943 2304 62-63 0.052455708677328516 2304 64-65 0.017233627824964515 2304 66-67 0.053209645826051144 2304 68-69 0.0052152779875882516 2304 70-71 0.06188226301734545 2304 72-73 0.050896726219846755 2304 74-75 -0.034657751825719174 2304 76-77 0.057159403269231746 2304 78-79 -0.05105794134677666 2304 80-81 0.06504997394463885 2304 82-83 -0.08672819358576334 2304 84-85 -0.07834733678579653 2304 86-87 -0.03483075571448779 2304 88-89 0.027771702966894907 2304 90-91 -0.060029932820675924 2304 92-93 -0.07696141858203731 2304 94-95 -0.07827380776362958 2304 96-97 -0.02038247262952808 2304 98-99 -0.19906955557885908 2304 100-101 -0.024122349237905905 2202 1 0.1393848910021731 2202 2 0.06486562808671437 2202 3 0.11224723935932701 2202 4 0.10721815414261471 2202 5 0.1436081410862755 2202 6 0.15753740976045805 2202 7 0.1501451769355242 2202 8 0.13415130098147188 2202 9 0.09201456847988254 2202 10-11 0.07909844157622103 2202 12-13 0.06027184464993951 2202 14-15 0.096117113630946 2202 16-17 0.07343509783971314 2202 18-19 0.022793649936993177 2202 20-21 0.037143240738529926 2202 22-23 0.07788056804533028 2202 24-25 0.03200097099774979 2202 26-27 0.0826668641808439 2202 28-29 0.09118907028084067 2202 30-31 0.04821577261613896 2202 32-33 0.08346707620886917 2202 34-35 -0.0025999677059189708 2202 36-37 -0.0038268553293292484 2202 38-39 0.021696290326318035 2202 40-41 0.07336184285190228 2202 42-43 -0.034514721161642115 2202 44-45 0.03581015691955258 2202 46-47 0.08935282136102529 2202 48-49 0.03075214397875925 2202 50-51 -0.20408751792417945 2202 52-53 0.006995376974224143 2202 54-55 0.20760924595953867 2202 56-57 0.1634753889095819 2202 58-59 0.14984727438651646 2202 60-61 0.17549622362739825 2202 62-63 0.14732933294425266 2202 64-65 0.11513328229699482 2202 66-67 0.11578909023619843 2202 68-69 0.02725712056216878 2202 70-71 0.10110267192742839 2202 72-73 0.12170655696129273 2202 74-75 0.011892345880625044 2202 76-77 0.0018761492093020138 2202 78-79 0.062265958214020145 2202 80-81 0.1740518875683037 2202 82-83 0.1279868489201874 2202 84-85 0.09321612803252322 2202 86-87 0.08573834733229546 2202 88-89 0.07032172248280943 2202 90-91 0.08133910044607973 2202 92-93 -0.028503892370586925 2202 94-95 -0.07071993164376522 2202 96-97 0.012144506760790819 2202 98-99 -0.028239853394946834 2202 100-101 -0.024122349237905905 1102 1 -0.0672707574962601 1102 2 -0.10816755002328904 1102 3 -0.07425636721839624 1102 4 -0.07006177211769682 1102 5 -0.061259380570028554 1102 6 -0.09713433234792035 1102 7 -0.14959639127278734 1102 8 -0.1774932023450475 1102 9 -0.09826149394946526 1102 10-11 -0.140553462906027 1102 12-13 -0.11806482084167413 1102 14-15 -0.23304738928950286 1102 16-17 -0.25123857241543135 1102 18-19 -0.2725492667497704 1102 20-21 -0.2359462132898571 1102 22-23 -0.167842724660332 1102 24-25 -0.11695260364605531 1102 26-27 -0.12669013405517404 1102 28-29 -0.0694804684988739 1102 30-31 -0.1391575041802966 1102 32-33 -0.1888073594841515 1102 34-35 -0.16650105301444285 1102 36-37 -0.1553647969051113 1102 38-39 -0.1381791105685295 1102 40-41 -0.16179752127749936 1102 42-43 -0.15599414323259708 1102 44-45 -0.17492351327197753 1102 46-47 -0.16912259924444584 1102 48-49 -0.1439362536927149 1102 50-51 -0.09714355255736962 1102 52-53 -0.048895543813770814 1102 54-55 -0.0020961573520779098 1102 56-57 -0.029378031286420025 1102 58-59 -0.0880249035775762 1102 60-61 -0.10231774003280947 1102 62-63 -0.04225889999713672 1102 64-65 -0.08316969584996059 1102 66-67 -0.0011979034542761724 1102 68-69 -0.16396624117859915 1102 70-71 -0.09019116814143757 1102 72-73 -0.046459749759947044 1102 74-75 0.003687777503820655 1102 76-77 0.04426374147703882 1102 78-79 0.030819651208702226 1102 80-81 0.05133411971873869 1102 82-83 -0.007271132585728424 1102 84-85 -0.026674010735234788 1102 86-87 -0.0016260566342083393 1102 88-89 0.03927719804275398 1102 90-91 0.01958600679548539 1102 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-0.3493414250693121 1213 86-87 -0.4261733090530413 1213 88-89 -0.40269864636299246 1213 90-91 -0.3477486780888839 1213 92-93 -0.42996254088650687 1213 94-95 -0.3884351070618024 1213 96-97 -0.3603175147020252 1213 98-99 -0.09616635549739172 1213 100-101 -0.024122349237905905 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 113.0 3 82.0 4 156.0 5 243.0 6 1428.0 7 11148.0 8 20633.0 9 20766.0 10 16252.0 11 12795.0 12 12155.0 13 12532.0 14 13960.0 15 15418.0 16 17732.0 17 20891.0 18 24947.0 19 28936.0 20 33920.0 21 39496.0 22 46387.0 23 54854.0 24 65975.0 25 80920.0 26 101981.0 27 135172.0 28 155787.0 29 187635.0 30 246992.0 31 336429.0 32 473699.0 33 710898.0 34 1199211.0 35 2536662.0 36 5326673.0 37 2459858.0 38 5149.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.114139045474648 22.71816691076648 31.258772535682922 25.90892150807595 2 26.786047277067563 22.376332656153537 30.520215778793048 20.31740428798586 3 21.414479000687738 20.729999551399715 37.747537049218245 20.107984398694303 4 21.149823557090173 20.503908306805204 37.01426211039972 21.332006025704906 5 28.147575568686428 19.80636766327916 32.375605616570155 19.670451151464256 6 29.471100205800255 20.398108415688917 31.011817859734197 19.11897351877663 7 28.305323053527353 25.843997850683536 26.311435345603595 19.539243750185513 8 26.952891126389915 21.91810468869727 30.66898969200845 20.46001449290437 9 22.722491971665697 21.002202980757932 35.03022354228154 21.245081505294824 10-11 27.455074498978593 21.848969532140746 29.03928985439349 21.656666114487166 12-13 26.737154112021777 25.858519307253513 25.0704315521146 22.333895028610115 14-15 26.50758401498426 23.585615010392573 26.506529723443723 23.400271251179444 16-17 26.02007206218133 23.38920079847158 25.959904076605554 24.630823062741534 18-19 25.683959732649203 24.022930645375247 26.651198075934353 23.641911546041193 20-21 24.220402151403057 24.78636755766536 27.1983451791451 23.79488511178648 22-23 24.39761910839194 24.77099635330949 26.68654927748696 24.144835260811618 24-25 24.649814724583802 24.67598736971332 26.44408185063871 24.230116055064165 26-27 24.477982387435837 24.583743777020576 26.803210538061112 24.135063297482475 28-29 24.72906480818063 24.812748006411667 26.086969489098987 24.371217696308715 30-31 24.791141989734474 24.634226215374095 26.070451929361333 24.504179865530098 32-33 24.5956106033466 25.043598397113364 25.90646202432382 24.454328975216217 34-35 24.45763351409499 25.212189191388568 25.68498104357892 24.645196250937516 36-37 24.475429970188493 25.25212200118902 25.93807144459459 24.334376584027897 38-39 24.684488783010718 25.014360027788857 25.779330747071167 24.52182044212926 40-41 24.387279651456474 25.650856779283032 25.55211277005703 24.409750799203472 42-43 24.359101453779576 25.462568436416916 25.7529462692614 24.425383840542107 44-45 24.66623519026045 25.037140681835645 26.10144182623112 24.195182301672784 46-47 24.14010645408968 25.37961580556122 26.09952764935232 24.38075009099678 48-49 24.396847586573326 25.072164025691546 26.140173912500458 24.390814475234667 50-51 24.20246745727706 25.292965067855928 26.46139822856532 24.043169246301694 52-53 24.28077530307077 25.54737326639967 26.20988163157511 23.96196979895445 54-55 24.12684699622031 25.29563856552844 26.02745162840202 24.550062809849234 56-57 24.195494721180193 25.59249115802756 26.109503332592027 24.102510788200224 58-59 24.03218598629333 25.551789175462698 26.343090906006832 24.072933932237138 60-61 24.49190339635795 25.413412590288736 25.630352557280116 24.464331456073197 62-63 24.149940748818523 25.682122224245262 26.0296546934175 24.138282333518717 64-65 24.393459416158443 25.7356606298952 25.920051437119575 23.950828516826782 66-67 24.743282381488964 25.892682869298834 25.44063577967283 23.923398969539374 68-69 24.699150074022004 25.79948103062502 25.700416098389766 23.800952796963205 70-71 24.456571773513822 26.316294830283415 25.444420324377663 23.782713071825096 72-73 24.766062507648417 25.84009446436386 25.43226562129286 23.961577406694865 74-75 24.60157022179809 25.751571387270467 25.511525825998106 24.135332564933336 76-77 24.729083612949495 25.491719634700594 25.473671877440562 24.305524874909352 78-79 24.9347619482237 25.329664634223786 25.35888627080315 24.37668714674937 80-81 24.739304933583718 25.273432188062078 25.547307277812752 24.43995560054145 82-83 24.35709422082622 25.756805847543056 25.58489769876338 24.301202232867343 84-85 24.425385290194704 25.680988174655837 25.511021688439083 24.38260484671037 86-87 24.10694472134656 25.75654229303897 25.73581217616585 24.40070080944862 88-89 24.133079691845765 26.421703669383955 25.212148216787643 24.233068421982633 90-91 24.21438861312715 25.87193997688165 25.425608053244552 24.488063356746643 92-93 23.994596152498147 26.25881312598524 25.34560673007566 24.40098399144095 94-95 24.160014940659895 26.593866668457245 24.93207660247223 24.314041788410627 96-97 24.106032371022245 26.090691824288726 25.156347974525232 24.646927830163794 98-99 24.57209807636088 25.866428896516595 25.263979300501504 24.29749372662102 100-101 32.01359818449771 49.7626646655154 5.9844669674421125 12.239270182544777 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 18397.0 1 10716.0 2 4067.0 3 4605.0 4 4473.5 5 3841.5 6 2751.5 7 2934.0 8 3593.5 9 3969.0 10 3731.5 11 3321.0 12 3079.5 13 3116.0 14 3234.0 15 3347.0 16 3460.5 17 3573.5 18 3621.5 19 3685.0 20 3807.5 21 3909.0 22 4229.5 23 4722.5 24 5282.5 25 5983.5 26 6825.0 27 7892.0 28 9215.0 29 10671.0 30 12963.0 31 17320.0 32 37100.5 33 65386.5 34 84374.0 35 122534.5 36 173917.5 37 238017.5 38 287377.0 39 296599.0 40 295044.0 41 315262.5 42 371940.5 43 449067.5 44 538099.5 45 651553.0 46 766211.5 47 853526.0 48 947309.0 49 992766.0 50 940933.0 51 872212.0 52 828006.5 53 787248.5 54 713125.0 55 626217.5 56 528530.5 57 407617.0 58 321375.0 59 252803.0 60 177957.0 61 115991.5 62 72998.5 63 42309.0 64 22803.5 65 12592.5 66 8004.5 67 5939.0 68 4653.5 69 3668.5 70 3001.5 71 2621.0 72 2373.5 73 2091.5 74 1687.5 75 1299.5 76 1088.0 77 1020.0 78 923.0 79 757.0 80 581.0 81 410.5 82 321.0 83 260.5 84 186.5 85 153.5 86 141.5 87 135.0 88 128.0 89 93.5 90 76.0 91 78.0 92 62.5 93 50.0 94 42.5 95 35.0 96 21.5 97 11.0 98 11.0 99 9.5 100 3.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 3.049650035330889E-4 2 0.016433455076748947 3 0.03636014564851328 4 0.01170649752198607 5 0.00447744073369035 6 0.05262032515507297 7 0.05833841897131839 8 0.04875974545125637 9 0.05774235100986735 10-11 0.06421246080073413 12-13 0.07290396340142717 14-15 0.07361092772779933 16-17 0.06298566976379422 18-19 0.0727584119224682 20-21 0.07021819206349372 22-23 0.07228363686014963 24-25 0.07041919172491325 26-27 0.06967410677309945 28-29 0.07218660254084365 30-31 0.08227817174866586 32-33 0.08070482957134743 34-35 0.09362425608465828 36-37 0.09346137704868039 38-39 0.1133430159721955 40-41 0.09596694179361702 42-43 0.09041519252475329 44-45 0.11178007032908843 46-47 0.10595454565932567 48-49 0.1091809367762496 50-51 0.09294848136092018 52-53 0.10194494896514632 54-55 0.10905617836571334 56-57 0.09775861118937391 58-59 0.0946881680856203 60-61 0.10175434583793815 62-63 0.09290689522407476 64-65 0.09282025743898013 66-67 0.093274239432876 68-69 0.11526290928989245 70-71 0.09623032066030468 72-73 0.09105284662304974 74-75 0.3883452079081584 76-77 0.3805686003180646 78-79 0.4047405423594657 80-81 0.432457702566939 82-83 0.4282193821201098 84-85 0.41010861952392885 86-87 0.3817364776611402 88-89 0.3691809298452268 90-91 0.36843237938200923 92-93 0.3797680671837903 94-95 0.3906636350372907 96-97 0.35722144999076444 98-99 0.3649703334896279 100-101 99.61670404220716 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4427885E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.20891226962862 #Duplication Level Percentage of deduplicated Percentage of total 1 74.93996557826114 12.896352931252848 2 10.934819017755082 3.763526823216276 3 3.2506951462552247 1.678227827616412 4 1.9773761614353804 1.3611397154478586 5 1.2395331116518729 1.0665508286858427 6 0.9453218900490427 0.9760776883448091 7 0.6314536725532873 0.7606641597313024 8 0.5196937535123882 0.7154691369014952 9 0.4694254043920703 0.7270470541186268 >10 3.394724891689762 11.95484059759455 >50 0.6608380015743367 8.110569691384415 >100 0.9014901352129835 34.15241431498933 >500 0.10086676344927266 11.641061835331627 >1k 0.033343630186280844 9.632303572330539 >5k 3.7256278291741807E-4 0.3773292530120797 >10k+ 8.027923901637974E-5 0.18642457004197738 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC 15901 0.11021019366317378 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.6634454738168484E-4 0.0 0.0 2 1.6634454738168484E-4 0.0 0.0 3 1.6634454738168484E-4 0.0 0.0 4 1.7327557018925505E-4 0.0 0.0 5 1.8020659299682526E-4 0.0 0.0 6 1.8020659299682526E-4 0.0 0.0 7 1.8020659299682526E-4 0.0 0.0 8 2.0099966141953586E-4 0.0 0.0 9 2.1486170703467625E-4 0.0 0.0 10-11 2.633788666876677E-4 0.0 0.0 12-13 3.708097202050058E-4 0.0 0.0 14-15 5.128956877601949E-4 0.0 0.0 16-17 6.688437009305245E-4 0.0 0.0 18-19 8.837054079652007E-4 0.0 0.0 20-21 0.0011886704114982896 0.0 0.0 22-23 0.0015178939948578742 0.0 0.0 24-25 0.0018644451352363843 0.0 0.0 26-27 0.0022075307642111094 0.0 0.0 28-29 0.0027550815660091553 0.0 0.0 30-31 0.0032402531625390692 0.0 0.0 32-33 0.0039368209546998744 0.0 3.465511403785101E-6 34-35 0.0047893367600310086 0.0 6.931022807570202E-6 36-37 0.0056557146109772844 0.0 6.931022807570202E-6 38-39 0.006948350364589127 0.0 6.931022807570202E-6 40-41 0.0083969341313713 0.0 6.931022807570202E-6 42-43 0.010091569207822214 0.0 6.931022807570202E-6 44-45 0.01223672076676519 6.931022807570202E-6 6.931022807570202E-6 46-47 0.014821992273988876 6.931022807570202E-6 1.7327557018925503E-5 48-49 0.0183290898146194 6.931022807570202E-6 2.0793068422710605E-5 50-51 0.022418393271085815 6.931022807570202E-6 2.0793068422710605E-5 52-53 0.02743645378376664 6.931022807570202E-6 2.0793068422710605E-5 54-55 0.03345258158073758 6.931022807570202E-6 2.0793068422710605E-5 56-57 0.04086184496203013 6.931022807570202E-6 2.0793068422710605E-5 58-59 0.05023605330926882 6.931022807570202E-6 2.0793068422710605E-5 60-61 0.06171382707860508 6.931022807570202E-6 2.0793068422710605E-5 62-63 0.07508030456300421 6.931022807570202E-6 2.0793068422710605E-5 64-65 0.0918776383371506 6.931022807570202E-6 2.0793068422710605E-5 66-67 0.11269149982828391 6.931022807570202E-6 2.0793068422710605E-5 68-69 0.13772288869782368 6.931022807570202E-6 2.0793068422710605E-5 70-71 0.1682401821195553 6.931022807570202E-6 2.0793068422710605E-5 72-73 0.2049434133970433 6.931022807570202E-6 2.0793068422710605E-5 74-75 0.24908016663565033 6.931022807570202E-6 2.7724091230280807E-5 76-77 0.30162771605124383 1.3862045615140404E-5 2.7724091230280807E-5 78-79 0.3662560382204322 1.7327557018925503E-5 2.7724091230280807E-5 80-81 0.44277106450460346 2.0793068422710605E-5 2.7724091230280807E-5 82-83 0.5345204789198139 2.0793068422710605E-5 2.7724091230280807E-5 84-85 0.6434934850118365 2.0793068422710605E-5 2.7724091230280807E-5 86-87 0.7713188731404499 2.0793068422710605E-5 2.7724091230280807E-5 88-89 0.8798829488868257 2.0793068422710605E-5 2.7724091230280807E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCGA 495 3.0827268E-6 365.1188 94-95 GCTAGTG 510 0.0016439381 236.25333 94-95 CGTCGTA 595 0.0026077572 202.50284 94-95 TATGCTA 955 4.24503E-5 189.25003 94-95 CGACGCA 680 0.0038885195 177.18999 94-95 CCGAACA 680 0.0038885195 177.18999 94-95 TCGAAAA 750 0.00521271 160.65227 94-95 TCCACTG 770 0.005639545 156.47948 94-95 GCAAACG 815 0.006683468 147.83951 94-95 ATATTAG 1440 2.1803545E-4 125.50957 94-95 GAGTAGA 2150 0.0010733658 84.062225 94-95 AGTGTCG 735 0.0 81.96544 94-95 CAAGTGA 2235 0.0012520314 80.865234 94-95 CAGTCGG 765 2.3900934E-4 78.75111 94-95 TGACGTA 1010 3.956302E-9 59.648113 94-95 GATGTGC 1020 0.0014827701 59.06333 94-95 CTTTGTG 144755 0.0 58.79822 1 GTACAGA 3175 0.005035896 56.92403 94-95 GTTCGGG 1070 1.335502E-8 56.303364 94-95 TTTGTGT 151735 0.0 56.088085 2 >>END_MODULE