FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l03n02_7765.34200000035c6c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l03n02_7765.34200000035c6c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14427885
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC159010.11021019366317378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA4953.0827268E-6365.118894-95
GCTAGTG5100.0016439381236.2533394-95
CGTCGTA5950.0026077572202.5028494-95
TATGCTA9554.24503E-5189.2500394-95
CGACGCA6800.0038885195177.1899994-95
CCGAACA6800.0038885195177.1899994-95
TCGAAAA7500.00521271160.6522794-95
TCCACTG7700.005639545156.4794894-95
GCAAACG8150.006683468147.8395194-95
ATATTAG14402.1803545E-4125.5095794-95
GAGTAGA21500.001073365884.06222594-95
AGTGTCG7350.081.9654494-95
CAAGTGA22350.001252031480.86523494-95
CAGTCGG7652.3900934E-478.7511194-95
TGACGTA10103.956302E-959.64811394-95
GATGTGC10200.001482770159.0633394-95
CTTTGTG1447550.058.798221
GTACAGA31750.00503589656.9240394-95
GTTCGGG10701.335502E-856.30336494-95
TTTGTGT1517350.056.0880852
Produced by FastQC (version 0.11.2)