##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l03n02_7331.34200000035c1c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19808495 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.408290180551326 33.0 33.0 33.0 33.0 33.0 2 32.40921084615464 33.0 33.0 33.0 33.0 33.0 3 32.39459050271108 33.0 33.0 33.0 33.0 33.0 4 36.24535362227166 37.0 37.0 37.0 37.0 37.0 5 36.22474105175583 37.0 37.0 37.0 37.0 37.0 6 36.20475063855179 37.0 37.0 37.0 37.0 37.0 7 36.17421187222956 37.0 37.0 37.0 37.0 37.0 8 36.206396043717604 37.0 37.0 37.0 37.0 37.0 9 36.19070373594763 37.0 37.0 37.0 37.0 37.0 10-11 36.18168952260129 37.0 37.0 37.0 37.0 37.0 12-13 36.14774643404257 37.0 37.0 37.0 37.0 37.0 14-15 38.120417023100444 40.0 37.0 40.0 37.0 40.0 16-17 38.12246864287266 40.0 37.0 40.0 37.0 40.0 18-19 38.07077180775218 40.0 37.0 40.0 37.0 40.0 20-21 38.02239672928206 40.0 37.0 40.0 37.0 40.0 22-23 37.953060416755534 40.0 37.0 40.0 37.0 40.0 24-25 37.887245043098936 40.0 37.0 40.0 33.0 40.0 26-27 37.83730924535155 40.0 37.0 40.0 33.0 40.0 28-29 37.76221805341597 40.0 37.0 40.0 33.0 40.0 30-31 37.701515334708674 40.0 37.0 40.0 33.0 40.0 32-33 37.64287105103139 40.0 37.0 40.0 33.0 40.0 34-35 37.565028312347806 40.0 37.0 40.0 33.0 40.0 36-37 37.448326437722805 40.0 37.0 40.0 33.0 40.0 38-39 37.32287404974482 40.0 37.0 40.0 33.0 40.0 40-41 37.23406142162744 40.0 37.0 40.0 33.0 40.0 42-43 37.13218755387524 40.0 37.0 40.0 33.0 40.0 44-45 36.950434598893054 38.5 37.0 40.0 33.0 40.0 46-47 36.77006688292069 37.0 37.0 40.0 33.0 40.0 48-49 36.57764820093601 37.0 37.0 40.0 33.0 40.0 50-51 35.22936573424685 37.0 35.0 38.5 30.0 38.5 52-53 35.587197311052655 37.0 35.0 38.5 33.0 40.0 54-55 36.2169364961851 37.0 37.0 40.0 33.0 40.0 56-57 36.12175458054739 37.0 37.0 40.0 33.0 40.0 58-59 35.88748143157771 37.0 37.0 40.0 33.0 40.0 60-61 35.62954532891065 37.0 37.0 37.0 33.0 40.0 62-63 35.36622370856544 37.0 33.0 37.0 33.0 40.0 64-65 35.130775558668134 37.0 33.0 37.0 33.0 40.0 66-67 34.89504195043591 37.0 33.0 37.0 33.0 38.5 68-69 34.65534648139599 37.0 33.0 37.0 27.0 37.0 70-71 34.45372308698869 37.0 33.0 37.0 27.0 37.0 72-73 34.273961752268406 37.0 33.0 37.0 27.0 37.0 74-75 33.987871541982365 37.0 33.0 37.0 27.0 37.0 76-77 33.8832272971773 37.0 33.0 37.0 27.0 37.0 78-79 33.74035475688587 37.0 33.0 37.0 27.0 37.0 80-81 33.596577049392195 37.0 33.0 37.0 27.0 37.0 82-83 33.48487262661803 37.0 33.0 37.0 27.0 37.0 84-85 33.37909187447103 37.0 33.0 37.0 27.0 37.0 86-87 33.2737606769217 37.0 33.0 37.0 27.0 37.0 88-89 33.15354720285413 37.0 33.0 37.0 27.0 37.0 90-91 33.084414817985916 37.0 33.0 37.0 27.0 37.0 92-93 32.9764572220151 35.0 33.0 37.0 27.0 37.0 94-95 32.85684386925912 33.0 33.0 37.0 27.0 37.0 96-97 32.73883270788619 33.0 33.0 37.0 27.0 37.0 98-99 31.227394711208497 33.0 30.0 35.0 21.0 37.0 100-101 2.019737945765188 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.05940006936295816 2306 2 0.11425365673894561 2306 3 0.12219960328528856 2306 4 0.13891244438663364 2306 5 0.1769131919992546 2306 6 0.14360206344021975 2306 7 0.1284559043282556 2306 8 0.09636671110378359 2306 9 0.1425379903443158 2306 10-11 0.12951568396268698 2306 12-13 0.11747446348743296 2306 14-15 0.16200167856960235 2306 16-17 0.15083775062353055 2306 18-19 0.1356532970176687 2306 20-21 0.14078088316733073 2306 22-23 0.1129422746493205 2306 24-25 0.13877717891305963 2306 26-27 0.05764148084561782 2306 28-29 0.12409897739638609 2306 30-31 0.05665277703940319 2306 32-33 0.03233422358167104 2306 34-35 0.07949262801939483 2306 36-37 0.02551483699684809 2306 38-39 -0.07497700854532496 2306 40-41 -0.015007913688677377 2306 42-43 -0.027641391232933188 2306 44-45 0.013400597623508759 2306 46-47 0.0015030785044700679 2306 48-49 -0.07363126239121698 2306 50-51 -0.03185846625460442 2306 52-53 -0.011661995971138595 2306 54-55 0.040637912682150557 2306 56-57 0.12849849520030432 2306 58-59 0.07109543755602488 2306 60-61 -0.07219014612660857 2306 62-63 0.02930541889663374 2306 64-65 0.10752845882347373 2306 66-67 0.03255586442939773 2306 68-69 -0.0048666961912502416 2306 70-71 -0.10795942653359702 2306 72-73 0.004643231236741485 2306 74-75 -0.019383196051443008 2306 76-77 0.01832517951437751 2306 78-79 -0.0165629309415678 2306 80-81 0.060945059776138066 2306 82-83 -0.035558919152798296 2306 84-85 -0.1494104715826765 2306 86-87 -0.10493865058866447 2306 88-89 -0.225502463897314 2306 90-91 -0.19359492571765458 2306 92-93 -0.23248348755450365 2306 94-95 -0.3679067739449806 2306 96-97 -0.27298734436168104 2306 98-99 -0.4810023103735084 2306 100-101 -0.023868859417998767 2307 1 0.04996117089563512 2307 2 0.04245719674852921 2307 3 0.02007194256536593 2307 4 0.0438134620297177 2307 5 0.08273755569923935 2307 6 0.08049176509253897 2307 7 0.0711079657980136 2307 8 0.04321989466962606 2307 9 0.054921448903975545 2307 10-11 0.04733591379262947 2307 12-13 0.016676813226702336 2307 14-15 0.021596219754933088 2307 16-17 0.011434208592476125 2307 18-19 0.006867383678752503 2307 20-21 -0.05775348036974748 2307 22-23 -0.10850356808612815 2307 24-25 -0.11817459591443225 2307 26-27 -0.21253019104739934 2307 28-29 -0.11993586894885055 2307 30-31 -0.22115622391052625 2307 32-33 -0.12996764246722847 2307 34-35 -0.1515122638321742 2307 36-37 -0.15232158230159598 2307 38-39 -0.13180296861560947 2307 40-41 -0.1678100591831324 2307 42-43 -0.1162470329245835 2307 44-45 -0.09630436458700586 2307 46-47 -0.15251088054007056 2307 48-49 -0.09067254836664063 2307 50-51 -0.09063578678591 2307 52-53 -0.03516340469559509 2307 54-55 0.07407683594895076 2307 56-57 0.016759485923806494 2307 58-59 0.0022415483167748107 2307 60-61 0.023899273707876034 2307 62-63 0.016643592375444882 2307 64-65 -0.038089903085456456 2307 66-67 -0.041903251857149826 2307 68-69 0.006360534014746122 2307 70-71 0.017057236695066536 2307 72-73 0.01144359992217403 2307 74-75 0.04093713163916135 2307 76-77 -0.010186390566758519 2307 78-79 -0.052432241247842626 2307 80-81 -0.008697306698955742 2307 82-83 -0.13320551293009686 2307 84-85 -0.09827739634674515 2307 86-87 -0.01512573670627404 2307 88-89 -0.14144161579507397 2307 90-91 -0.13654483697272468 2307 92-93 -0.18455909543933302 2307 94-95 -0.18474306660636586 2307 96-97 -0.1962721667149978 2307 98-99 -0.49139678291919253 2307 100-101 -0.023868859417998767 2201 1 0.1302544514985584 2201 2 0.12859767866270033 2201 3 0.10720633998960238 2201 4 0.1513706555701475 2201 5 0.12804563098896438 2201 6 0.13075665896521826 2201 7 0.12273146430300841 2201 8 0.09667295262542552 2201 9 0.0571875461605984 2201 10-11 0.08772352751910972 2201 12-13 0.08742523912030009 2201 14-15 0.14860571485772311 2201 16-17 0.15915969645473638 2201 18-19 0.12756873864245222 2201 20-21 0.13427406235278738 2201 22-23 0.10564112137323889 2201 24-25 0.08558027118888845 2201 26-27 0.04316083768551238 2201 28-29 0.08449416889964567 2201 30-31 0.052231772327374415 2201 32-33 0.06801403741045675 2201 34-35 0.14848332325557578 2201 36-37 0.10224262635255599 2201 38-39 0.05156432637881636 2201 40-41 0.06297616352127022 2201 42-43 0.05479240891559556 2201 44-45 0.06341088995552724 2201 46-47 0.05478923469956243 2201 48-49 -0.004037000405539004 2201 50-51 -0.007021387219765529 2201 52-53 0.08523638382271059 2201 54-55 0.22278731810637709 2201 56-57 0.13413339677689606 2201 58-59 0.17651013588234576 2201 60-61 0.0783145127641589 2201 62-63 0.13022475929917476 2201 64-65 0.07954997588901591 2201 66-67 0.07086464560525485 2201 68-69 0.06837449684907426 2201 70-71 0.09680672970522153 2201 72-73 0.02939054860264889 2201 74-75 0.07178932303487073 2201 76-77 0.10776807946461986 2201 78-79 -0.046731029490473475 2201 80-81 -0.07984297720619082 2201 82-83 0.02149401443427479 2201 84-85 -0.02132198236107996 2201 86-87 -0.09309754331665232 2201 88-89 -0.05888587645493004 2201 90-91 -0.1657949126048237 2201 92-93 -0.19817082069370429 2201 94-95 -0.18223286856900245 2201 96-97 -0.1848683304565455 2201 98-99 -0.552737102462018 2201 100-101 -0.023868859417998767 1101 1 0.07828647239037423 1101 2 0.05401023125661908 1101 3 0.04088347373207313 1101 4 0.09364811390185679 1101 5 0.10366699997051398 1101 6 0.06914993535900038 1101 7 0.05295765820373077 1101 8 0.07585182307619931 1101 9 0.04801304336947254 1101 10-11 0.04355622124179348 1101 12-13 -0.03147265029270585 1101 14-15 0.056265486598071845 1101 16-17 0.03020767260257884 1101 18-19 0.027310477093465124 1101 20-21 0.03744365297584551 1101 22-23 0.0668656464504096 1101 24-25 0.10158366191006962 1101 26-27 0.08070233477315725 1101 28-29 0.05891070459711756 1101 30-31 -0.02374718956814803 1101 32-33 0.03937586357048417 1101 34-35 0.014269312909618748 1101 36-37 -0.0875576661985491 1101 38-39 -0.09909190431347525 1101 40-41 -0.02620137434109182 1101 42-43 -0.05904902939422385 1101 44-45 -0.05230237242145819 1101 46-47 -0.03155537755021243 1101 48-49 -0.08633662535866904 1101 50-51 -0.14063488906482746 1101 52-53 -0.04930819404960829 1101 54-55 0.08223249234832508 1101 56-57 0.11219815120141874 1101 58-59 0.07540793209535934 1101 60-61 0.09340499437726635 1101 62-63 0.07794119655481069 1101 64-65 0.058461967331119524 1101 66-67 0.10133188960323025 1101 68-69 -0.039466475475727236 1101 70-71 -0.04734173020938215 1101 72-73 0.03821326243239298 1101 74-75 -0.008152876883457338 1101 76-77 0.009035507600728465 1101 78-79 -0.06814227473671508 1101 80-81 -0.07434175693456524 1101 82-83 -0.060203122170193524 1101 84-85 -0.15125153695635163 1101 86-87 -0.12604067913863304 1101 88-89 -0.23907607735654324 1101 90-91 -0.2511664604096495 1101 92-93 -0.2577159360863064 1101 94-95 -0.2701343880282181 1101 96-97 -0.24418877317181398 1101 98-99 -0.40836449882281656 1101 100-101 -0.023868859417998767 2304 1 0.10848026792589849 2304 2 0.06562549528253214 2304 3 0.08551394515378519 2304 4 0.12705263265861788 2304 5 0.11847865606179653 2304 6 0.09358105599309852 2304 7 0.07105254765291846 2304 8 0.11696631076686259 2304 9 0.07796032924422747 2304 10-11 0.0644386204106695 2304 12-13 0.06911888332341931 2304 14-15 0.09143018478861364 2304 16-17 0.1118219413021535 2304 18-19 0.1134969460460411 2304 20-21 0.08761219288076205 2304 22-23 0.14402721124396578 2304 24-25 0.08968683201135264 2304 26-27 0.03506775918400962 2304 28-29 0.0738233161024624 2304 30-31 0.022095098211273978 2304 32-33 0.09061336645807216 2304 34-35 0.08079201668500957 2304 36-37 0.07206840790091462 2304 38-39 0.07444173319679948 2304 40-41 0.10972394443480482 2304 42-43 0.08927836831426106 2304 44-45 0.0787677608930224 2304 46-47 0.0693901321706889 2304 48-49 0.03144270788540382 2304 50-51 0.09828283844268526 2304 52-53 0.2279107325732781 2304 54-55 0.2680916301220506 2304 56-57 0.11442522660220789 2304 58-59 0.12610979425268454 2304 60-61 0.1684864091225151 2304 62-63 0.13401343544680344 2304 64-65 0.10276025220594676 2304 66-67 0.05958912886258361 2304 68-69 0.09548932682619693 2304 70-71 0.13055429392009898 2304 72-73 0.14181705687599333 2304 74-75 0.02326488893233858 2304 76-77 0.068195655842203 2304 78-79 -0.006534905937485291 2304 80-81 0.0573450157007116 2304 82-83 -0.08417569749158105 2304 84-85 0.00689614061644761 2304 86-87 0.07135431221058042 2304 88-89 0.1203341295737701 2304 90-91 0.052319028964319614 2304 92-93 0.012693014745757125 2304 94-95 -0.011267763906708694 2304 96-97 0.02180905133116795 2304 98-99 -0.22241799267318285 2304 100-101 -0.023868859417998767 2202 1 0.09182473632385069 2202 2 0.07738621332605078 2202 3 0.11776341657527212 2202 4 0.09691950562149287 2202 5 0.13205177621254194 2202 6 0.13822169387361782 2202 7 0.1578243108615922 2202 8 0.10634386390517392 2202 9 0.08364302346991082 2202 10-11 0.08433088786910758 2202 12-13 0.07635476063399693 2202 14-15 0.08355292404687731 2202 16-17 0.0854596952609441 2202 18-19 0.03653815877073896 2202 20-21 0.08135339279102283 2202 22-23 0.06837293136868539 2202 24-25 0.05931258787911986 2202 26-27 0.00587047444687272 2202 28-29 0.07506856339134771 2202 30-31 0.09330067799549369 2202 32-33 0.04930653667814511 2202 34-35 -0.0353809645066292 2202 36-37 0.015410716984995076 2202 38-39 0.01600610465718688 2202 40-41 0.10053940257195393 2202 42-43 -0.017460696703665235 2202 44-45 0.018907565196009557 2202 46-47 0.040191765122798984 2202 48-49 -0.025827992973709968 2202 50-51 -0.19212010198430818 2202 52-53 0.00823358373062888 2202 54-55 0.17216463536880866 2202 56-57 0.08868747706666369 2202 58-59 0.11474552488185452 2202 60-61 0.1549366841499875 2202 62-63 0.12898333304332255 2202 64-65 0.06321493514462162 2202 66-67 0.09695910041562428 2202 68-69 0.017941003121570986 2202 70-71 0.07320585533129531 2202 72-73 0.07443664308643605 2202 74-75 0.051978270450554476 2202 76-77 -0.06213270790593839 2202 78-79 0.039808492932806416 2202 80-81 0.11585809580513029 2202 82-83 0.12108851720224578 2202 84-85 0.04163371178334074 2202 86-87 0.04311702272905649 2202 88-89 0.04458923614774335 2202 90-91 0.023304979278918836 2202 92-93 -0.07654973107197094 2202 94-95 0.005617909807625665 2202 96-97 0.028879080904374632 2202 98-99 -0.022976755346615363 2202 100-101 -0.023868859417998767 1102 1 -0.051815398631362086 1102 2 -0.07649079854940055 1102 3 -0.08756765701482294 1102 4 -0.050556526132254476 1102 5 -0.05194983668439335 1102 6 -0.10056689699663934 1102 7 -0.09405299811209744 1102 8 -0.10952849456997171 1102 9 -0.08511292591573039 1102 10-11 -0.0846936814622623 1102 12-13 -0.05733803088231326 1102 14-15 -0.19793555976091426 1102 16-17 -0.15039173372468895 1102 18-19 -0.22403220193376683 1102 20-21 -0.1963142747975084 1102 22-23 -0.12602354349260025 1102 24-25 -0.09852035900330947 1102 26-27 -0.12300764308073298 1102 28-29 -0.0849700490596561 1102 30-31 -0.11607886910459797 1102 32-33 -0.12046297264366501 1102 34-35 -0.09784692274182305 1102 36-37 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-0.45944684087779564 1213 98-99 -0.10264480373133011 1213 100-101 -0.023868859417998767 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 137.0 3 110.0 4 200.0 5 281.0 6 1804.0 7 13764.0 8 26252.0 9 26743.0 10 20772.0 11 16178.0 12 14923.0 13 15603.0 14 17134.0 15 19253.0 16 22649.0 17 26364.0 18 30931.0 19 36368.0 20 42893.0 21 50656.0 22 59562.0 23 70332.0 24 85038.0 25 104694.0 26 134089.0 27 178698.0 28 206877.0 29 249366.0 30 328973.0 31 448646.0 32 637837.0 33 974079.0 34 1689137.0 35 3589844.0 36 7359718.0 37 3296409.0 38 12181.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.821204455211795 22.225459338268973 30.785698884632062 26.16763732188717 2 27.9529163846092 22.313084825871137 29.406108780344876 20.32789000917479 3 21.788426696627923 20.475818788528507 36.63816185906856 21.097592655775006 4 21.511336360671265 20.671906445362005 35.84479692701975 21.971960266946983 5 27.998034894010036 19.988057099142285 31.531095944416844 20.48281206243084 6 29.89962922935599 20.642515569240548 29.651867947727133 19.805987253676328 7 28.315410231084414 26.77759361175236 24.467547531857033 20.439448625306188 8 26.60799844114386 22.34408951139426 29.615074559072863 21.432837488389012 9 23.077903778949143 21.188891389212795 33.4172583468515 22.315946484986565 10-11 27.57568896000792 21.977878871648937 27.54944406719814 22.896988101145002 12-13 26.663686716882257 25.426046010620013 24.38152191336621 23.52874535913152 14-15 26.215168318856463 23.718497048852765 25.82364470321207 24.242689929078704 16-17 25.719510189335587 23.4930429718882 25.320424679577847 25.467022159198365 18-19 25.7333044094857 24.025638828709457 25.68728912080058 24.553767641004264 20-21 24.822267049463534 24.378604665307773 26.198182198167448 24.60094608706125 22-23 24.918936694755107 24.53518227899023 25.546419684337017 24.999461341917645 24-25 25.415133993374265 24.41744943428455 25.263610376271018 24.903806196070168 26-27 25.084836137882295 24.48233771308491 25.598519530554892 24.834306618477907 28-29 25.053612403345927 25.05442322945573 24.969723475206766 24.92224089199157 30-31 25.383385438628775 24.81929370218608 24.816744771559733 24.980576087625415 32-33 25.085492561745472 25.084156227343573 24.975670630199136 24.85468058071182 34-35 24.910360633219764 25.238451326834426 24.90970374947222 24.94148429047359 36-37 25.14955430568349 24.948679844389748 24.998923724398665 24.902842125528096 38-39 25.08287308454149 24.634523771438836 25.14422527065186 25.138377873367812 40-41 24.851358103694007 25.056290627831224 24.741681103092414 25.350670165382354 42-43 24.850240129157594 24.934151940456214 24.90080094927921 25.314806981106987 44-45 25.2160118041499 24.729648059013876 24.879501956328316 25.1748381805079 46-47 24.76894888964782 24.85434135336051 25.130000203152953 25.246709553838713 48-49 24.924771682061373 24.581750868190102 24.990467432631092 25.503010017117433 50-51 24.805546727689727 24.893828754595344 25.429397457670643 24.871227060044284 52-53 24.92538411216802 24.66704669551642 25.52772335388792 24.879845838427638 54-55 24.690591757698176 24.847776006494673 24.988262666414922 25.47336956939223 56-57 24.838938073041756 25.058129897116483 25.235203459124605 24.86772857071716 58-59 24.650404023555463 25.249785763650323 25.202534767582048 24.897275445212163 60-61 25.222747473743944 25.049032351535814 24.718222686504667 25.009997488215575 62-63 24.773236205382503 25.256702360679935 25.26819523300213 24.701866200935427 64-65 25.039391285080832 25.114303320095534 25.012168689644476 24.834136705179166 66-67 25.159010427514662 25.209539655489703 25.152709432786175 24.478740484209467 68-69 25.034287075837607 24.86418406159976 25.520713366262438 24.580815496300197 70-71 24.872058707011483 25.373598984857757 25.10548796115359 24.64885434697717 72-73 24.82721168799359 24.93443011645626 25.42145427136174 24.81690392418842 74-75 24.85598977332782 24.877812268965858 25.373676029290998 24.892521928415324 76-77 24.744538184799357 24.949765060999106 25.225227736157553 25.08046901804399 78-79 24.9886808502593 24.812592515754115 25.046054018315267 25.152672615671317 80-81 24.872389902939005 24.672465093710873 25.404738539681638 25.050406463668484 82-83 24.632493632634173 25.463792559039206 25.229550154400638 24.674163653925987 84-85 24.82988651531269 25.149567438111387 25.07951344102775 24.94103260554817 86-87 24.729227122764087 25.222841276443585 25.279772879668343 24.768158721123978 88-89 24.823422571083704 26.26271431972062 24.55202768894606 24.36183542024962 90-91 24.896412194658456 25.273658697749035 24.79468203199584 25.035247075596672 92-93 24.69596250079179 25.589961360613163 24.82802305694559 24.886053081649457 94-95 24.814888826867428 25.76118688468061 24.47320964258153 24.950714645870434 96-97 24.755204642699788 25.220270541284464 24.78261346460656 25.24191135140919 98-99 25.234013736453253 25.065423474352244 24.709594862764796 24.99096792642971 100-101 31.93098306528917 50.05258813505166 5.845271061880925 12.171157737778252 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8981.0 1 5486.0 2 2764.5 3 3083.0 4 2769.5 5 2415.5 6 1904.0 7 1970.0 8 2139.5 9 2176.5 10 2111.5 11 2146.5 12 2264.0 13 2470.5 14 2837.0 15 3154.5 16 3352.5 17 3411.0 18 3336.5 19 3435.5 20 3624.5 21 3889.0 22 4615.5 23 5720.5 24 6846.0 25 8355.0 26 10521.5 27 12923.5 28 15614.0 29 19042.0 30 23928.0 31 31393.0 32 41932.5 33 54687.0 34 71810.0 35 94559.0 36 121278.0 37 150762.0 38 186723.0 39 250463.5 40 329999.5 41 403299.0 42 472028.0 43 567033.5 44 710779.0 45 884835.5 46 1088905.5 47 1259237.5 48 1353549.0 49 1345402.0 50 1260164.0 51 1192957.0 52 1140584.5 53 1075159.5 54 1011768.0 55 979771.5 56 902528.0 57 725683.5 58 539046.5 59 402835.0 60 308265.5 61 228831.0 62 156550.5 63 101138.5 64 63253.0 65 38590.5 66 23244.5 67 15948.5 68 11845.0 69 8984.0 70 6939.0 71 5398.0 72 4039.0 73 2997.0 74 2316.5 75 1765.0 76 1321.0 77 1083.0 78 877.0 79 659.5 80 494.5 81 370.0 82 308.5 83 263.0 84 202.0 85 149.5 86 125.5 87 112.5 88 94.0 89 79.0 90 63.5 91 50.5 92 45.5 93 34.0 94 24.0 95 20.5 96 16.0 97 11.5 98 6.0 99 4.5 100 2.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 2.7261031188891433E-4 2 0.015821494767775137 3 0.03494965165198063 4 0.011686905037459939 5 0.0046040852674572195 6 0.0504934877687578 7 0.056243546013970275 8 0.04726255073896326 9 0.0558346305461369 10-11 0.0619052583247743 12-13 0.07056568406635638 14-15 0.07147943344509515 16-17 0.06114043494975262 18-19 0.07061111911833787 20-21 0.06809199790291993 22-23 0.07034608131511254 24-25 0.06846052665788087 26-27 0.0677613316912769 28-29 0.07001289093391497 30-31 0.08017267339088607 32-33 0.07847643145024395 34-35 0.09128911610902292 36-37 0.0909963124406978 38-39 0.11062930323580869 40-41 0.09372998806825052 42-43 0.08823991928715433 44-45 0.10881947366521282 46-47 0.10323348644104462 48-49 0.10634831167133092 50-51 0.0905848728033099 52-53 0.0991821943060288 54-55 0.10706012748570752 56-57 0.0958149521202898 58-59 0.09307117981451898 60-61 0.09982838171198771 62-63 0.09070855711148172 64-65 0.09061768700751874 66-67 0.09071360545059078 68-69 0.11232302100689628 70-71 0.09369212552493261 72-73 0.08878766408048668 74-75 0.38651598720649905 76-77 0.3784664104971125 78-79 0.40265805150769907 80-81 0.4307747761755752 82-83 0.42649883295020646 84-85 0.40818598283211316 86-87 0.3794685058102597 88-89 0.3668981414287153 90-91 0.3658051760116051 92-93 0.3773381067062389 94-95 0.3885908545803202 96-97 0.35502192367466584 98-99 0.3629074293630081 100-101 99.62080915284074 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.9808495E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.50983720150152 #Duplication Level Percentage of deduplicated Percentage of total 1 79.42695475640949 18.673147757342136 2 8.881974868156195 4.176275663563602 3 3.4219535271324695 2.413487110019648 4 1.7771722073668974 1.6712411709691541 5 1.0270351920015959 1.2072715082085186 6 0.7408123615697663 1.0449826810419052 7 0.5151536081612822 0.8477824223146494 8 0.2964545591392634 0.557567873840559 9 0.29651938056155064 0.6274010127682916 >10 2.514438173222736 12.385540276766141 >50 0.49685954287035744 8.33188265083181 >100 0.5058113157960274 24.523116681059633 >500 0.06772420249462889 10.835667810547731 >1k 0.03074440527457687 12.105288328577817 >5k 3.91899843218818E-4 0.5993470521485367 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.464018341625651E-4 0.0 0.0 2 1.464018341625651E-4 0.0 0.0 3 1.5649851238067305E-4 0.0 0.0 4 1.6659519059878097E-4 0.0 0.0 5 1.766918688168889E-4 0.0 0.0 6 1.9183688614405082E-4 0.0 0.0 7 2.0193356436215876E-4 0.0 0.0 8 2.120302425802667E-4 0.0 0.0 9 2.271752599074286E-4 0.0 0.0 10-11 2.9785200743418416E-4 0.0 0.0 12-13 3.6095624629735876E-4 0.0 0.0 14-15 4.7959221536012707E-4 0.0 0.0 16-17 6.51235745067962E-4 0.0 0.0 18-19 8.10258427003162E-4 0.0 0.0 20-21 9.995711435926858E-4 0.0 0.0 22-23 0.0012519880990453842 0.0 0.0 24-25 0.0015473159369250415 0.0 0.0 26-27 0.0018274987574775367 0.0 0.0 28-29 0.00211777825624814 0.0 0.0 30-31 0.0024989278589817145 0.0 0.0 32-33 0.0030264792958778547 0.0 0.0 34-35 0.003566651580546629 0.0 0.0 36-37 0.004429917568194858 0.0 0.0 38-39 0.005550648850404839 0.0 0.0 40-41 0.006868265357867925 0.0 0.0 42-43 0.008690715776236407 0.0 0.0 44-45 0.010911984984220153 0.0 0.0 46-47 0.013766820750390173 0.0 0.0 48-49 0.01737638321336376 0.0 0.0 50-51 0.022051145228347738 0.0 0.0 52-53 0.027627035774297846 0.0 0.0 54-55 0.034649275474991914 0.0 0.0 56-57 0.04294117246161306 0.0 0.0 58-59 0.05350734621686302 0.0 0.0 60-61 0.06694854909471921 0.0 0.0 62-63 0.08361059232415183 0.0 0.0 64-65 0.10400840649428439 0.0 0.0 66-67 0.12918447363113655 0.0 0.0 68-69 0.16020651745627318 0.0 0.0 70-71 0.19855622549820165 0.0 0.0 72-73 0.24579101037206513 0.0 0.0 74-75 0.3027413238613029 0.0 0.0 76-77 0.371921238842224 0.0 0.0 78-79 0.45688731021715684 0.0 0.0 80-81 0.5583967888524595 0.0 0.0 82-83 0.6789233609115685 0.0 0.0 84-85 0.8237021540505728 0.0 0.0 86-87 0.9945455220096227 0.0 0.0 88-89 1.1418787747378083 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCGA 1175 3.6379788E-12 311.79797 94-95 GGGCACA 1045 1.9696745E-7 233.72417 94-95 TAGGGCA 1350 1.599447E-4 135.68987 94-95 GCTCACA 1720 4.1941862E-4 106.50077 94-95 GCATTCA 2345 1.1011349E-5 104.15427 94-95 CTCTCGG 1800 5.0253904E-4 101.7674 94-95 ACATCCG 2060 8.591574E-4 88.92297 94-95 CGAGGTA 2330 0.0014011839 78.61859 94-95 GTCTCCA 2590 0.0021320384 70.72638 94-95 CTTTGTG 159180 0.0 68.06855 1 TTTGTGT 168735 0.0 64.23377 2 TTGTGTT 192025 0.0 58.01277 3 TGACGTA 3280 0.005434715 55.84796 94-95 AACTTTG 17390 0.0 55.4703 2 TAACTTT 17445 0.0 55.24175 1 TGTGTTT 205265 0.0 54.614887 4 ACTTTGT 40585 0.0 54.481983 1 GTGTTTG 236515 0.0 47.37879 5 TCTCGCA 5555 7.8628724E-4 43.96791 94-95 CGTGACA 4840 0.0 37.847378 94-95 >>END_MODULE