FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l03n02_7331.34200000035c1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l03n02_7331.34200000035c1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19808495
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA11753.6379788E-12311.7979794-95
GGGCACA10451.9696745E-7233.7241794-95
TAGGGCA13501.599447E-4135.6898794-95
GCTCACA17204.1941862E-4106.5007794-95
GCATTCA23451.1011349E-5104.1542794-95
CTCTCGG18005.0253904E-4101.767494-95
ACATCCG20608.591574E-488.9229794-95
CGAGGTA23300.001401183978.6185994-95
GTCTCCA25900.002132038470.7263894-95
CTTTGTG1591800.068.068551
TTTGTGT1687350.064.233772
TTGTGTT1920250.058.012773
TGACGTA32800.00543471555.8479694-95
AACTTTG173900.055.47032
TAACTTT174450.055.241751
TGTGTTT2052650.054.6148874
ACTTTGT405850.054.4819831
GTGTTTG2365150.047.378795
TCTCGCA55557.8628724E-443.9679194-95
CGTGACA48400.037.84737894-95
Produced by FastQC (version 0.11.2)