##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	C8364ACXX_l03n02_6957.34200000035bd7.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26458576
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44056452622394	33.0	33.0	33.0	33.0	33.0
2	32.439727406342655	33.0	33.0	33.0	33.0	33.0
3	32.4302682049102	33.0	33.0	33.0	33.0	33.0
4	36.29071095889665	37.0	37.0	37.0	37.0	37.0
5	36.27134393022512	37.0	37.0	37.0	37.0	37.0
6	36.237315417126	37.0	37.0	37.0	37.0	37.0
7	36.20240503494973	37.0	37.0	37.0	37.0	37.0
8	36.236184517262004	37.0	37.0	37.0	37.0	37.0
9	36.219761940325135	37.0	37.0	37.0	37.0	37.0
10-11	36.218471697040684	37.0	37.0	37.0	37.0	37.0
12-13	36.17494066952054	37.0	37.0	37.0	37.0	37.0
14-15	38.13997508785053	40.0	37.0	40.0	37.0	40.0
16-17	38.1442906073252	40.0	37.0	40.0	37.0	40.0
18-19	38.08212467670218	40.0	37.0	40.0	37.0	40.0
20-21	38.03622819686139	40.0	37.0	40.0	37.0	40.0
22-23	37.97696225223912	40.0	37.0	40.0	37.0	40.0
24-25	37.90990182162487	40.0	37.0	40.0	35.0	40.0
26-27	37.85539355935104	40.0	37.0	40.0	33.0	40.0
28-29	37.76824504841077	40.0	37.0	40.0	33.0	40.0
30-31	37.71322604436459	40.0	37.0	40.0	33.0	40.0
32-33	37.65286504836844	40.0	37.0	40.0	33.0	40.0
34-35	37.57154203990419	40.0	37.0	40.0	33.0	40.0
36-37	37.43564092413742	40.0	37.0	40.0	33.0	40.0
38-39	37.31382403195093	40.0	37.0	40.0	33.0	40.0
40-41	37.223332276083184	40.0	37.0	40.0	33.0	40.0
42-43	37.11831379360703	40.0	37.0	40.0	33.0	40.0
44-45	36.95692247383231	40.0	37.0	40.0	33.0	40.0
46-47	36.79942104593989	37.0	37.0	40.0	33.0	40.0
48-49	36.626327868136215	37.0	37.0	40.0	33.0	40.0
50-51	35.28788316121019	37.0	35.0	38.5	30.0	38.5
52-53	35.647640428570305	37.0	35.0	38.5	33.0	40.0
54-55	36.31348527600276	37.0	37.0	40.0	33.0	40.0
56-57	36.232252162777016	37.0	37.0	40.0	33.0	40.0
58-59	36.002644492281064	37.0	37.0	40.0	33.0	40.0
60-61	35.751623386685665	37.0	37.0	38.5	33.0	40.0
62-63	35.49567590107646	37.0	35.0	37.0	33.0	40.0
64-65	35.25197865901778	37.0	33.0	37.0	33.0	40.0
66-67	35.00949076019813	37.0	33.0	37.0	30.0	40.0
68-69	34.75444485372153	37.0	33.0	37.0	27.0	38.5
70-71	34.538844985459534	37.0	33.0	37.0	27.0	37.0
72-73	34.3386294107438	37.0	33.0	37.0	27.0	37.0
74-75	34.03333962492917	37.0	33.0	37.0	27.0	37.0
76-77	33.915978395813895	37.0	33.0	37.0	27.0	37.0
78-79	33.76153975557868	37.0	33.0	37.0	27.0	37.0
80-81	33.59341944555142	37.0	33.0	37.0	27.0	37.0
82-83	33.470974968569735	37.0	33.0	37.0	27.0	37.0
84-85	33.35646702982051	37.0	33.0	37.0	27.0	37.0
86-87	33.23966660186096	37.0	33.0	37.0	27.0	37.0
88-89	33.1180259285307	37.0	33.0	37.0	27.0	37.0
90-91	33.03857078325001	37.0	33.0	37.0	27.0	37.0
92-93	32.92941216866697	37.0	33.0	37.0	27.0	37.0
94-95	32.79920555815249	35.0	33.0	37.0	27.0	37.0
96-97	32.6748321224846	33.0	33.0	37.0	27.0	37.0
98-99	31.20150090465942	33.0	30.0	37.0	18.5	37.0
100-101	2.0198491029902743	2.0	2.0	2.0	2.0	2.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2306	1	0.07675839731480494
2306	2	0.1235392337399901
2306	3	0.13606642078990916
2306	4	0.12726014405139807
2306	5	0.17152196347926463
2306	6	0.15592680290613004
2306	7	0.15972377769410429
2306	8	0.12076421473754095
2306	9	0.1323899178055612
2306	10-11	0.0896439138076559
2306	12-13	0.0892841192956979
2306	14-15	0.13852917793438735
2306	16-17	0.13914364443512284
2306	18-19	0.13209367157961083
2306	20-21	0.09342774436252199
2306	22-23	0.10999088582060068
2306	24-25	0.09169245671638038
2306	26-27	0.05505732729479007
2306	28-29	0.07717865016074654
2306	30-31	0.03762059029804021
2306	32-33	0.0243153773460989
2306	34-35	0.041629818377209915
2306	36-37	0.01768497205217301
2306	38-39	-0.06683826737234
2306	40-41	-0.0117322464492986
2306	42-43	-0.022547572532744198
2306	44-45	-0.020660710433766383
2306	46-47	-0.005887421884580135
2306	48-49	-0.0725261344617607
2306	50-51	-0.0543148757666998
2306	52-53	-0.04945078350199594
2306	54-55	0.020129450040322183
2306	56-57	0.0764299861465858
2306	58-59	0.07374465455568924
2306	60-61	-0.09355138702278509
2306	62-63	-0.029075628013671917
2306	64-65	0.021413347704076102
2306	66-67	-0.042275958678075654
2306	68-69	0.011483214110320716
2306	70-71	-0.1181473924704477
2306	72-73	-0.023201460510328786
2306	74-75	0.015736101220845455
2306	76-77	0.041601564128058044
2306	78-79	-0.01486853915206865
2306	80-81	0.06591839272541478
2306	82-83	0.005440470421383736
2306	84-85	-0.10676477381982608
2306	86-87	-0.1337289568036084
2306	88-89	-0.25638727810651574
2306	90-91	-0.20768688758765563
2306	92-93	-0.21582264700268894
2306	94-95	-0.3128652038762283
2306	96-97	-0.30189382987938274
2306	98-99	-0.5605388413196231
2306	100-101	-0.02385862009985873
2307	1	0.0530463156998735
2307	2	0.018732381945156362
2307	3	0.007756526898980098
2307	4	0.017779517374918896
2307	5	0.03449530953509594
2307	6	0.04871211166083356
2307	7	0.05886895631986988
2307	8	0.009673184184912031
2307	9	0.005445306214447498
2307	10-11	-6.841390790626178E-4
2307	12-13	-0.03656258193379358
2307	14-15	0.006586861414881184
2307	16-17	-0.05211419776030368
2307	18-19	-0.08905768914377887
2307	20-21	-0.1322368779825709
2307	22-23	-0.18065043923518687
2307	24-25	-0.17512092194737505
2307	26-27	-0.2821390682475027
2307	28-29	-0.21606112121384058
2307	30-31	-0.2982252484025736
2307	32-33	-0.2340756062719791
2307	34-35	-0.22635606137641417
2307	36-37	-0.250983828752787
2307	38-39	-0.24829353858301317
2307	40-41	-0.24825281457952997
2307	42-43	-0.23652376937865682
2307	44-45	-0.20627133462717495
2307	46-47	-0.2703526906217846
2307	48-49	-0.2556249015860459
2307	50-51	-0.2191485164109892
2307	52-53	-0.15508042537592814
2307	54-55	-0.08724900036047956
2307	56-57	-0.09864164519463259
2307	58-59	-0.09307343798452195
2307	60-61	-0.10663891724583152
2307	62-63	-0.10530235249780162
2307	64-65	-0.1389203128216181
2307	66-67	-0.13748812696634616
2307	68-69	-0.12388875867461024
2307	70-71	-0.07823785108713821
2307	72-73	-0.09655323930794424
2307	74-75	-0.03330989979857435
2307	76-77	-0.05140398981308891
2307	78-79	-0.09458405358387267
2307	80-81	-0.057611445427887986
2307	82-83	-0.11144179162425871
2307	84-85	-0.1498156657863987
2307	86-87	-0.07195877005409557
2307	88-89	-0.1390077885866603
2307	90-91	-0.18554368232704377
2307	92-93	-0.26254287254797504
2307	94-95	-0.27447615434100214
2307	96-97	-0.19051603610571277
2307	98-99	-0.45756732002891454
2307	100-101	-0.02385862009985873
2201	1	0.16498391495272813
2201	2	0.15755332668422284
2201	3	0.12629494889461768
2201	4	0.15071756431080274
2201	5	0.18443050089106805
2201	6	0.14766366831078415
2201	7	0.14870721318810354
2201	8	0.11804360946163683
2201	9	0.036121023171439504
2201	10-11	0.09223852864322879
2201	12-13	0.07656867640598364
2201	14-15	0.13058227649941756
2201	16-17	0.1263147863262617
2201	18-19	0.11154396071486872
2201	20-21	0.13452171690658332
2201	22-23	0.12695123490151872
2201	24-25	0.12673971614155022
2201	26-27	0.061547536107752876
2201	28-29	0.1468924916005534
2201	30-31	0.03215016323085251
2201	32-33	0.02536788889786834
2201	34-35	0.0947344755804238
2201	36-37	0.054449996978270576
2201	38-39	0.004546209665441836
2201	40-41	-0.044892253591527265
2201	42-43	0.007553767050964666
2201	44-45	-0.021899107207552504
2201	46-47	-0.018921653685900708
2201	48-49	-0.0822921710490192
2201	50-51	-0.06557445173866228
2201	52-53	0.019187759724424325
2201	54-55	0.16290100843796296
2201	56-57	0.07276883666451539
2201	58-59	0.08403204589000524
2201	60-61	0.022026067041927888
2201	62-63	0.05960078427595761
2201	64-65	-0.006847154814437317
2201	66-67	-0.030478671151200842
2201	68-69	0.020627032823597347
2201	70-71	0.03585339900292439
2201	72-73	-0.014478929053773015
2201	74-75	0.02406120989230942
2201	76-77	0.02905438449783304
2201	78-79	-0.10536014541743555
2201	80-81	-0.09706725436073071
2201	82-83	-0.06142519328061269
2201	84-85	-0.07740129464908563
2201	86-87	-0.0973148029839308
2201	88-89	-0.0828447779295729
2201	90-91	-0.16779189542606332
2201	92-93	-0.3245982270656995
2201	94-95	-0.22770964536016436
2201	96-97	-0.2333255640294496
2201	98-99	-0.5562426512624015
2201	100-101	-0.02385862009985873
1101	1	0.09180414161666306
1101	2	0.04495397419976399
1101	3	0.0635826962553665
1101	4	0.14102932381966582
1101	5	0.12230113926588615
1101	6	0.08758337575970643
1101	7	0.11132966374778874
1101	8	0.10175098435916397
1101	9	0.0796999058428014
1101	10-11	0.0730611730018822
1101	12-13	-0.006497397322192455
1101	14-15	0.09118250126828542
1101	16-17	0.07513678780590283
1101	18-19	0.07022259609471604
1101	20-21	0.0841453852683074
1101	22-23	0.08567412542407027
1101	24-25	0.12870904719084564
1101	26-27	0.13347608907975683
1101	28-29	0.13521086267434157
1101	30-31	0.08113456595250312
1101	32-33	0.11758580176779532
1101	34-35	0.1194254664820491
1101	36-37	0.030352335915004858
1101	38-39	0.0036795467995389686
1101	40-41	0.027045411586904322
1101	42-43	0.058769234234532064
1101	44-45	0.049615864354898065
1101	46-47	0.048728996126349955
1101	48-49	-0.0416182954413955
1101	50-51	-0.06350678514041874
1101	52-53	0.06623293318518364
1101	54-55	0.1557845617693161
1101	56-57	0.16043485418389736
1101	58-59	0.13617919265171707
1101	60-61	0.13531526495981439
1101	62-63	0.06538600308648057
1101	64-65	0.07617396315097125
1101	66-67	0.1103822268967889
1101	68-69	0.02208152835510191
1101	70-71	0.04772310917638123
1101	72-73	0.06878035412883321
1101	74-75	0.05117860973589217
1101	76-77	0.08496212393005464
1101	78-79	0.01439687009434465
1101	80-81	-0.04848781417820902
1101	82-83	-0.030853815319652256
1101	84-85	-0.06572199144980573
1101	86-87	-0.02474228482808627
1101	88-89	-0.20401512650803966
1101	90-91	-0.16713060129641377
1101	92-93	-0.16974449132803926
1101	94-95	-0.14115025514934842
1101	96-97	-0.16225879445703129
1101	98-99	-0.2493562243772658
1101	100-101	-0.02385862009985873
2304	1	0.09588870502822289
2304	2	0.05788967974140746
2304	3	0.0695155095162363
2304	4	0.12757635984566207
2304	5	0.12944942712442753
2304	6	0.10835425897619899
2304	7	0.08232509679284306
2304	8	0.07124223357299542
2304	9	0.05907214368986047
2304	10-11	0.043274461076869386
2304	12-13	0.0662327576528412
2304	14-15	0.0697952853617565
2304	16-17	0.08181900824671118
2304	18-19	0.0890547717805461
2304	20-21	0.09594065399674889
2304	22-23	0.0886342930292372
2304	24-25	0.07536023694050442
2304	26-27	-0.006667679632940349
2304	28-29	0.03216664550909343
2304	30-31	0.028130674903842134
2304	32-33	0.06817645690623664
2304	34-35	0.060444680700058484
2304	36-37	0.09648866835667746
2304	38-39	0.07493666106983454
2304	40-41	0.039720553323427055
2304	42-43	0.06314101525282467
2304	44-45	0.0634060765820621
2304	46-47	0.01744443761742076
2304	48-49	0.006355158793532212
2304	50-51	0.12189230759764769
2304	52-53	0.16089208911954245
2304	54-55	0.19493058005169672
2304	56-57	0.08549736788828
2304	58-59	0.08122867082807517
2304	60-61	0.09545981971842821
2304	62-63	0.05789799312758248
2304	64-65	0.041556508480233845
2304	66-67	0.07984535379326019
2304	68-69	0.08083073161067489
2304	70-71	0.10227207138660077
2304	72-73	0.0707544764816177
2304	74-75	-0.02609279201205794
2304	76-77	0.0738796929690011
2304	78-79	0.021450777167842716
2304	80-81	0.0864392049608469
2304	82-83	-0.06636640882153699
2304	84-85	-0.032793886483467816
2304	86-87	-0.0025262309283959894
2304	88-89	0.08837390803150669
2304	90-91	0.05390422167439368
2304	92-93	-0.03631095078113589
2304	94-95	-0.009069162719690382
2304	96-97	0.00391729614673153
2304	98-99	-0.13732693163070664
2304	100-101	-0.02385862009985873
2202	1	0.08645209751082206
2202	2	0.07557167582586999
2202	3	0.06768255670245082
2202	4	0.0655643731714477
2202	5	0.10704208915970526
2202	6	0.11893773568549904
2202	7	0.12219182937484163
2202	8	0.10663319514161174
2202	9	0.06560569304485142
2202	10-11	0.03069817364353611
2202	12-13	0.03404729895734704
2202	14-15	0.05666283266583605
2202	16-17	0.04198442950604431
2202	18-19	0.015485285309665642
2202	20-21	0.07843499021408462
2202	22-23	0.05724115326680135
2202	24-25	0.03712523120152156
2202	26-27	0.014074621206987104
2202	28-29	0.06764205565443149
2202	30-31	0.02487129984658054
2202	32-33	0.02244199956606252
2202	34-35	-0.031592161716872624
2202	36-37	-0.018935057959375
2202	38-39	-0.013001348640521826
2202	40-41	0.04840072353760405
2202	42-43	-0.055290709536663485
2202	44-45	-0.027207798701560648
2202	46-47	-0.014632750893532886
2202	48-49	-0.04799461128042992
2202	50-51	-0.21167611089779825
2202	52-53	-0.02712658986323646
2202	54-55	0.16388471349990397
2202	56-57	0.09786444884574763
2202	58-59	0.09648701016944727
2202	60-61	0.07065215277748393
2202	62-63	0.09047363323847435
2202	64-65	0.04238650232525032
2202	66-67	0.0756789838974612
2202	68-69	-0.01473134637570439
2202	70-71	0.02419061429577596
2202	72-73	0.056796308415123065
2202	74-75	0.04179495943503042
2202	76-77	-0.052034002695968695
2202	78-79	0.03544145169229296
2202	80-81	0.08273026944466721
2202	82-83	0.10136711947593113
2202	84-85	0.02451081992104065
2202	86-87	0.05960387787941812
2202	88-89	-0.001387183003103587
2202	90-91	-0.018780977759057293
2202	92-93	-0.09954740540147355
2202	94-95	-0.07701478148857177
2202	96-97	0.04289034450222573
2202	98-99	-0.02957944841865867
2202	100-101	-0.02385862009985873
1102	1	-0.06128080122133639
1102	2	-0.09458888160337864
1102	3	-0.06941173316756277
1102	4	-0.04839730459482183
1102	5	-0.0675096523794565
1102	6	-0.0816758624114442
1102	7	-0.09026478219353606
1102	8	-0.10773162418249882
1102	9	-0.06916222018346474
1102	10-11	-0.11666359362940426
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1213	94-95	-0.415775406359046
1213	96-97	-0.48629631734452516
1213	98-99	-0.0959619811859227
1213	100-101	-0.02385862009985873
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	155.0
3	142.0
4	295.0
5	436.0
6	2307.0
7	15576.0
8	29617.0
9	29561.0
10	23869.0
11	20945.0
12	21316.0
13	23384.0
14	25719.0
15	29323.0
16	33576.0
17	39463.0
18	46544.0
19	54644.0
20	63688.0
21	74286.0
22	86143.0
23	100413.0
24	119758.0
25	145670.0
26	184969.0
27	244778.0
28	286946.0
29	343830.0
30	450287.0
31	603773.0
32	834871.0
33	1231131.0
34	2087052.0
35	4463550.0
36	9611764.0
37	5105576.0
38	23219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.790029416243417	21.35996617496729	31.784676015970813	28.06532839281848
2	25.837132147787205	20.261616388651436	35.032175858251584	18.869075605309774
3	20.012861633729194	18.974350307529082	43.834147611085186	17.178640447656537
4	19.037987956375026	17.985446499006443	43.95068849603617	19.025877048582355
5	30.58212210420294	17.4617203769402	35.20843525049539	16.747722268361464
6	29.998126309432717	17.835140565552038	35.874159840741974	16.29257328427327
7	31.67127118414606	24.98180277750424	26.740364661447707	16.60656137690199
8	27.501128995854092	20.513224140285207	34.0286213447164	17.957025519144302
9	21.2032532045143	18.78872069052638	40.80800156982505	19.200024535134272
10-11	28.90671407387148	19.985222058464085	30.913319975601045	20.19474389206339
12-13	27.289335297590796	27.042444685856164	24.041686020346486	21.62653399620656
14-15	27.253070282335717	23.123091025472863	25.824498475543567	23.799340216647856
16-17	26.457297749069674	22.598143511935376	25.338499909236678	25.606058829758265
18-19	26.23550280185508	23.874557210300413	26.213450897599117	23.67648909024539
20-21	24.135790121373848	25.17431282319926	27.102826837229	23.587070218197898
22-23	24.64513862419006	25.123063996228122	26.465357171142312	23.7664402084395
24-25	24.720006465855022	25.315698321419255	26.135656558107673	23.828638654618047
26-27	24.893759951081282	25.115804886985522	26.18941730280341	23.801017859129793
28-29	24.630155989808532	25.614563514258883	25.75797520454311	23.997305291389477
30-31	24.99726049864485	25.399799820654863	25.57162527948682	24.031314401213468
32-33	24.816911285499927	25.580840336897303	25.430670484947083	24.171577892655684
34-35	25.06258708360268	25.529360111805964	25.578608499359625	23.829444305231736
36-37	25.034117909216917	25.578468580966778	25.530302232158924	23.857111277657374
38-39	25.10229708097699	25.27950292463046	25.552199917432084	24.066000076960474
40-41	24.904684837891068	25.565313681237136	25.261544865355578	24.268456615516218
42-43	24.84832513238806	25.739537685485892	25.2772670523247	24.13487012980135
44-45	24.814009107133423	25.433115138954456	25.533029169452277	24.219846584459848
46-47	24.46996664792681	25.746708230944044	25.390277066785927	24.393048054343218
48-49	24.64566440701033	25.220206628770452	25.293120920681083	24.841008043538135
50-51	24.520605329204436	25.303772907296533	25.95744119850006	24.218180564998967
52-53	24.770985332025514	25.199203618707077	25.824462924896828	24.205348124370573
54-55	24.53394447153567	25.13086096264747	25.332731951629732	25.00246261418712
56-57	24.572136479225225	25.351937293915842	25.60239671100285	24.473529515856075
58-59	24.746651230883533	25.422230924879297	25.45706509203722	24.374052752199958
60-61	24.82631944661326	25.466426198729646	25.19369317025445	24.51356118440264
62-63	24.493324819204002	25.903282037973874	25.33393683782059	24.269456305001533
64-65	24.810509964991105	25.44330067489845	25.18502712896459	24.56116223114585
66-67	24.989094753925027	25.581577501392594	25.325401156253047	24.103926588429335
68-69	24.631044804198275	25.033197747407737	25.828502073366995	24.507255375026993
70-71	24.711177691145465	25.255574010334204	25.407728362942684	24.62551993557765
72-73	24.168285733524293	24.958876458389604	26.14870523828654	24.72413256979956
74-75	24.471798450847448	24.778596213322192	25.93936124211461	24.810244093715745
76-77	24.223585199654995	24.800222461510284	25.774667690625986	25.20152464820874
78-79	24.795427174229502	24.99719370771208	25.155858323944873	25.051520794113546
80-81	24.47391560462731	24.818426254141617	25.643144717684628	25.06451342354644
82-83	24.528580263658107	25.450117191987403	25.53248627101357	24.48881627334092
84-85	24.532953944197054	25.58789010036327	25.4033270793354	24.475828876104273
86-87	24.400332640667077	25.451108525895656	25.67880022024791	24.469758613189356
88-89	24.38219075434109	26.58162691054654	24.901185838224393	24.13499649688798
90-91	24.486604687691184	26.03636674562316	24.922850937782727	24.554177628902934
92-93	24.33506404734482	26.314884631807033	24.692711809778032	24.657339511070113
94-95	24.373083255235784	26.459584069575744	24.530931401194977	24.6364012739935
96-97	24.23622213182942	26.083193230391743	24.553190971246035	25.1273936665328
98-99	24.658330280311763	25.858327078716975	24.173844396879097	25.309498244092165
100-101	32.05688463846906	49.94270861751678	5.7345945704634405	12.265812173550726
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11956.0
1	7218.0
2	3800.0
3	4904.5
4	5766.0
5	4973.5
6	3010.0
7	3004.5
8	3276.0
9	3320.0
10	3209.0
11	3371.5
12	3712.5
13	4010.5
14	4275.0
15	4520.0
16	4756.5
17	4924.0
18	4968.0
19	4992.0
20	5163.0
21	5513.5
22	5961.5
23	6867.5
24	7889.5
25	9200.5
26	11129.5
27	13301.5
28	16199.5
29	20128.5
30	25661.5
31	33525.5
32	49655.5
33	73597.0
34	104386.5
35	156568.5
36	224744.0
37	305647.0
38	394413.5
39	501016.0
40	620669.0
41	749861.5
42	873793.0
43	993532.0
44	1176489.5
45	1406833.0
46	1633715.5
47	1766574.0
48	1810839.0
49	1753054.0
50	1626735.0
51	1543191.5
52	1443791.0
53	1293806.0
54	1127589.0
55	996166.0
56	834666.0
57	628438.0
58	475677.5
59	373631.0
60	298655.0
61	233878.5
62	166655.0
63	116449.0
64	81019.0
65	54996.5
66	39961.5
67	32157.5
68	26900.0
69	23077.0
70	21638.0
71	20904.0
72	21378.0
73	20581.0
74	17647.0
75	15067.5
76	12398.0
77	11030.5
78	11124.5
79	9223.5
80	6775.0
81	5154.5
82	4124.5
83	3371.5
84	2390.0
85	1901.5
86	1901.5
87	1935.5
88	1859.0
89	1596.5
90	1182.0
91	883.5
92	677.0
93	573.5
94	553.5
95	469.5
96	334.0
97	255.0
98	169.0
99	91.0
100	27.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	2.4566703816562163E-4
2	0.016138434661033912
3	0.03541384842479807
4	0.011663515073524743
5	0.004622319810408542
6	0.05080016399975569
7	0.056616803564938645
8	0.04727767662175017
9	0.05618216188203024
10-11	0.06222179152800967
12-13	0.07078612242775273
14-15	0.07176501108752036
16-17	0.06113707706718608
18-19	0.07061037600814193
20-21	0.06817071334451257
22-23	0.07057825031853567
24-25	0.06857511908426213
26-27	0.0679628412352955
28-29	0.07034203201260718
30-31	0.08043894728121423
32-33	0.07878730888616228
34-35	0.0914939639986672
36-37	0.09125774569273871
38-39	0.11121157843112948
40-41	0.09381646238255603
42-43	0.08848548765436205
44-45	0.1090553777346143
46-47	0.10350330267207124
48-49	0.10669319467532946
50-51	0.09096294524693997
52-53	0.09925893215114827
54-55	0.10707114396481504
56-57	0.09581581412393471
58-59	0.0935764645837327
60-61	0.10015845146012392
62-63	0.0910328658654948
64-65	0.09073239618035378
66-67	0.09090058361417486
68-69	0.11272337558907176
70-71	0.09408102688519594
72-73	0.0891809143470155
74-75	0.38906288834289493
76-77	0.3808141450998723
78-79	0.40470809918115014
80-81	0.43181651196950277
82-83	0.4275797004343696
84-85	0.41006742010605557
86-87	0.3817476798449017
88-89	0.3693660611213544
90-91	0.3682454414780296
92-93	0.37938360703916946
94-95	0.38997752562345006
96-97	0.3576458536544068
98-99	0.36552987583307583
100-101	99.6190252264521
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.6458576E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.381298759020034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.3849753972369	12.021412064054724
2	9.273060835035167	3.038095598985577
3	3.9867463577631095	1.9592424948885738
4	2.39959662347562	1.572340367611593
5	1.7050630139610432	1.3965573317325495
6	1.2180985775399726	1.1972422029971772
7	0.9009240509001801	1.0330814225887692
8	0.5017317901360752	0.657521468089359
9	0.45402101156013047	0.6693708449915087
>10	4.340892182350401	15.404819584142976
>50	0.8281898080189851	9.467795501309082
>100	0.8737206295101372	29.108243447840497
>500	0.09563692980477903	10.775141299598856
>1k	0.03605221340830284	9.916934943858521
>5k	0.001010138078430012	1.0794320374910757
>10k+	2.804412206333746E-4	0.7027693898191896
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.3054906658619874E-4	0.0	0.0
2	2.3432855948105446E-4	0.0	0.0
3	2.3810805237591017E-4	0.0	0.0
4	2.418875452707659E-4	0.0	0.0
5	2.4566703816562163E-4	0.0	0.0
6	2.5700551685018877E-4	0.0	0.0
7	2.7968247421932305E-4	0.0	0.0
8	3.023594315884574E-4	0.0	0.0
9	3.552723321164374E-4	0.0	0.0
10-11	5.102315408055218E-4	0.0	0.0
12-13	7.029856784431633E-4	0.0	0.0
14-15	9.032988018705164E-4	0.0	0.0
16-17	0.0011508555864835658	0.0	0.0
18-19	0.0014758919754411575	3.779492894855717E-6	0.0
20-21	0.0019048644190072816	3.779492894855717E-6	0.0
22-23	0.0023943087488910966	3.779492894855717E-6	0.0
24-25	0.002949894204434887	3.779492894855717E-6	0.0
26-27	0.0035565028140592298	3.779492894855717E-6	0.0
28-29	0.004304842407240662	3.779492894855717E-6	0.0
30-31	0.005107984647397501	3.779492894855717E-6	0.0
32-33	0.006079314321375421	3.779492894855717E-6	0.0
34-35	0.007224500668516704	3.779492894855717E-6	0.0
36-37	0.008554882167505915	3.779492894855717E-6	0.0
38-39	0.010338802813877813	3.779492894855717E-6	0.0
40-41	0.012563034382500404	3.779492894855717E-6	0.0
42-43	0.015388205321405052	3.779492894855717E-6	0.0
44-45	0.018906913206515726	3.779492894855717E-6	0.0
46-47	0.023396950765604316	3.779492894855717E-6	0.0
48-49	0.028771389662089145	3.779492894855717E-6	0.0
50-51	0.035283455919925544	3.779492894855717E-6	0.0
52-53	0.0438610150448006	3.779492894855717E-6	0.0
54-55	0.05427918720947038	3.779492894855717E-6	0.0
56-57	0.06724284783882549	3.779492894855717E-6	0.0
58-59	0.083313251627752	3.779492894855717E-6	0.0
60-61	0.10372629275286774	3.779492894855717E-6	0.0
62-63	0.12976699879842363	3.779492894855717E-6	0.0
64-65	0.16282244365683174	3.779492894855717E-6	0.0
66-67	0.2031817585345485	3.779492894855717E-6	0.0
68-69	0.2519561143426615	3.779492894855717E-6	0.0
70-71	0.311341396453082	3.779492894855717E-6	0.0
72-73	0.3785256621520372	3.779492894855717E-6	0.0
74-75	0.4580046182379581	3.779492894855717E-6	0.0
76-77	0.5503886528133638	3.779492894855717E-6	0.0
78-79	0.6621898321360908	3.779492894855717E-6	0.0
80-81	0.7936405950191726	3.779492894855717E-6	0.0
82-83	0.9513040308745263	3.779492894855717E-6	0.0
84-85	1.144368086929546	3.779492894855717E-6	0.0
86-87	1.3741518062045364	3.779492894855717E-6	0.0
88-89	1.5693947399134407	3.779492894855717E-6	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCTAG	1290	1.3550994E-4	141.47504	94-95
TCGAGTA	1440	2.099941E-4	126.73806	94-95
GCAAACG	2240	8.940411E-6	108.632614	94-95
GTATAGA	1755	4.613919E-4	103.990204	94-95
ATCTATG	1760	4.6664185E-4	103.69477	94-95
GATGACA	2950	3.5076228E-5	82.487144	94-95
TATCTCG	3205	5.2909014E-5	75.9242	94-95
ACTAACG	2455	0.0	74.339226	94-95
CGCAAAA	2915	0.0034586429	62.608166	94-95
CTTTGTG	465405	0.0	59.320374	1
GACTAAA	3105	0.0044413563	58.777073	94-95
TAACTTT	69745	0.0	56.973125	1
TTTGTGT	495970	0.0	55.667686	2
ACATCCA	7735	2.4627298E-8	55.053635	94-95
AACTTTG	73865	0.0	54.274277	2
AGATCGA	3435	0.006623641	53.130363	94-95
CTCACCG	5845	1.6428869E-5	52.03958	94-95
CTAACCA	4695	3.4972592E-4	51.828987	94-95
TTGTGTT	549785	0.0	51.362812	3
ACTTTGT	157135	0.0	51.03065	1
>>END_MODULE