FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l03n02_6957.34200000035bd7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l03n02_6957.34200000035bd7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26458576
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTAG12901.3550994E-4141.4750494-95
TCGAGTA14402.099941E-4126.7380694-95
GCAAACG22408.940411E-6108.63261494-95
GTATAGA17554.613919E-4103.99020494-95
ATCTATG17604.6664185E-4103.6947794-95
GATGACA29503.5076228E-582.48714494-95
TATCTCG32055.2909014E-575.924294-95
ACTAACG24550.074.33922694-95
CGCAAAA29150.003458642962.60816694-95
CTTTGTG4654050.059.3203741
GACTAAA31050.004441356358.77707394-95
TAACTTT697450.056.9731251
TTTGTGT4959700.055.6676862
ACATCCA77352.4627298E-855.05363594-95
AACTTTG738650.054.2742772
AGATCGA34350.00662364153.13036394-95
CTCACCG58451.6428869E-552.0395894-95
CTAACCA46953.4972592E-451.82898794-95
TTGTGTT5497850.051.3628123
ACTTTGT1571350.051.030651
Produced by FastQC (version 0.11.2)