##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l03n02_6929.34200000035b94.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15598456 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.398402572664885 33.0 33.0 33.0 33.0 33.0 2 32.39806170559445 33.0 33.0 33.0 33.0 33.0 3 32.381861832991675 33.0 33.0 33.0 33.0 33.0 4 36.23060288787557 37.0 37.0 37.0 37.0 37.0 5 36.20937931292687 37.0 37.0 37.0 37.0 37.0 6 36.19688544814948 37.0 37.0 37.0 37.0 37.0 7 36.16977379043156 37.0 37.0 37.0 37.0 37.0 8 36.1988121131989 37.0 37.0 37.0 37.0 37.0 9 36.18640703926081 37.0 37.0 37.0 37.0 37.0 10-11 36.17398356606577 37.0 37.0 37.0 37.0 37.0 12-13 36.14361588095642 37.0 37.0 37.0 37.0 37.0 14-15 38.151800633344735 40.0 37.0 40.0 37.0 40.0 16-17 38.15233417974189 40.0 37.0 40.0 37.0 40.0 18-19 38.10604321991869 40.0 37.0 40.0 37.0 40.0 20-21 38.06808112931177 40.0 37.0 40.0 37.0 40.0 22-23 38.007842827520875 40.0 37.0 40.0 37.0 40.0 24-25 37.947033090967466 40.0 37.0 40.0 37.0 40.0 26-27 37.90131654697106 40.0 37.0 40.0 35.0 40.0 28-29 37.83267244527279 40.0 37.0 40.0 33.0 40.0 30-31 37.77488929032463 40.0 37.0 40.0 33.0 40.0 32-33 37.72066674419571 40.0 37.0 40.0 33.0 40.0 34-35 37.6445772581594 40.0 37.0 40.0 33.0 40.0 36-37 37.53567455650739 40.0 37.0 40.0 33.0 40.0 38-39 37.42436228944711 40.0 37.0 40.0 33.0 40.0 40-41 37.34621259950344 40.0 37.0 40.0 33.0 40.0 42-43 37.25929229790435 40.0 37.0 40.0 33.0 40.0 44-45 37.096792432533064 40.0 37.0 40.0 33.0 40.0 46-47 36.93726484852091 38.5 37.0 40.0 33.0 40.0 48-49 36.76658994326105 37.0 37.0 40.0 33.0 40.0 50-51 35.46538590101481 37.0 35.0 38.5 30.0 38.5 52-53 35.813475961979826 37.0 37.0 38.5 33.0 40.0 54-55 36.46158199888502 37.0 37.0 40.0 33.0 40.0 56-57 36.38643497792346 37.0 37.0 40.0 33.0 40.0 58-59 36.165793332365716 37.0 37.0 40.0 33.0 40.0 60-61 35.91985931171649 37.0 37.0 40.0 33.0 40.0 62-63 35.653898469181826 37.0 37.0 37.0 33.0 40.0 64-65 35.41541675663284 37.0 35.0 37.0 33.0 40.0 66-67 35.1775674143646 37.0 33.0 37.0 33.0 40.0 68-69 34.92440585145094 37.0 33.0 37.0 33.0 40.0 70-71 34.710386880598946 37.0 33.0 37.0 27.0 37.0 72-73 34.51152989116359 37.0 33.0 37.0 27.0 37.0 74-75 34.20927010339997 37.0 33.0 37.0 27.0 37.0 76-77 34.0823432780783 37.0 33.0 37.0 27.0 37.0 78-79 33.92614900474765 37.0 33.0 37.0 27.0 37.0 80-81 33.77030406086347 37.0 33.0 37.0 27.0 37.0 82-83 33.65743795411546 37.0 33.0 37.0 27.0 37.0 84-85 33.54827346373256 37.0 33.0 37.0 27.0 37.0 86-87 33.447616898749466 37.0 33.0 37.0 27.0 37.0 88-89 33.33612317142158 37.0 33.0 37.0 27.0 37.0 90-91 33.26988825047812 37.0 33.0 37.0 27.0 37.0 92-93 33.166382877895096 37.0 33.0 37.0 27.0 37.0 94-95 33.051984824651875 37.0 33.0 37.0 27.0 37.0 96-97 32.935385015029695 37.0 33.0 37.0 27.0 37.0 98-99 31.49300443582365 33.0 30.0 37.0 21.0 37.0 100-101 2.0195153610075254 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.03456812756081007 2306 2 0.10845878052740687 2306 3 0.12324482194156872 2306 4 0.12034448763829175 2306 5 0.1594895625851862 2306 6 0.11126343158728247 2306 7 0.13771424550284905 2306 8 0.11628110075622544 2306 9 0.09568705196584659 2306 10-11 0.10552374127547637 2306 12-13 0.05663607868073228 2306 14-15 0.11931529917072226 2306 16-17 0.135149758470952 2306 18-19 0.13522838594465725 2306 20-21 0.11611495543475314 2306 22-23 0.11450273190479976 2306 24-25 0.11254016671202294 2306 26-27 0.03852983760781825 2306 28-29 0.043380704295685746 2306 30-31 0.02208622308558006 2306 32-33 0.02013128953795018 2306 34-35 0.059181368225900144 2306 36-37 -0.00390175780102453 2306 38-39 -0.03707308747427618 2306 40-41 0.02411873987813351 2306 42-43 -0.012757922132848876 2306 44-45 -0.008765947709079569 2306 46-47 -0.006662989411587716 2306 48-49 -0.07280086569621602 2306 50-51 -0.04538258305498033 2306 52-53 -0.046468302451231125 2306 54-55 0.033761814817651725 2306 56-57 0.09118506904929546 2306 58-59 0.045089482566552874 2306 60-61 -0.03198445950800988 2306 62-63 -0.015461019111008056 2306 64-65 0.07264809261217664 2306 66-67 -0.020495289545571893 2306 68-69 0.03553934697495009 2306 70-71 -0.05643455045017021 2306 72-73 -0.046486954355941634 2306 74-75 -0.0032310568548865604 2306 76-77 0.029167797543053098 2306 78-79 0.018241995005489287 2306 80-81 0.08900088232795866 2306 82-83 0.010665231920867768 2306 84-85 -0.12285333497297302 2306 86-87 -0.1396735310992696 2306 88-89 -0.240773861417658 2306 90-91 -0.1336508766623652 2306 92-93 -0.19590192285704688 2306 94-95 -0.3374110404291244 2306 96-97 -0.2603967814825836 2306 98-99 -0.45210192399554927 2306 100-101 -0.0239331559074607 2307 1 0.07180214662353279 2307 2 0.03366296160869098 2307 3 0.025914650767312253 2307 4 0.012786017256978255 2307 5 0.05625339112604877 2307 6 0.0331521659409546 2307 7 0.03073624673505293 2307 8 0.0479849013364273 2307 9 0.01690368559994937 2307 10-11 -0.021330860550669684 2307 12-13 -0.051042978721447696 2307 14-15 -0.01584676773598659 2307 16-17 -0.010340910412864446 2307 18-19 -0.03990175148746289 2307 20-21 -0.10241191149204809 2307 22-23 -0.14891823333690013 2307 24-25 -0.17762113457426665 2307 26-27 -0.24865079728994743 2307 28-29 -0.209969162878906 2307 30-31 -0.25589214499845525 2307 32-33 -0.12952748621117394 2307 34-35 -0.16460873141443955 2307 36-37 -0.18615948944882632 2307 38-39 -0.22196046189556995 2307 40-41 -0.22009816320240816 2307 42-43 -0.190745577243149 2307 44-45 -0.1611612067218644 2307 46-47 -0.21392038551415027 2307 48-49 -0.16233936615681444 2307 50-51 -0.14523047642982334 2307 52-53 -0.11542222104954902 2307 54-55 -0.009104276943368461 2307 56-57 -0.004607265827445417 2307 58-59 -0.020135799619772854 2307 60-61 0.010563023029561691 2307 62-63 -0.003971740899842757 2307 64-65 -0.06803711385494182 2307 66-67 -0.0363016206275546 2307 68-69 -0.06540769879097041 2307 70-71 -0.07031211699240458 2307 72-73 -0.06562790514826844 2307 74-75 -0.03756226324031786 2307 76-77 -0.08946984260842328 2307 78-79 -0.08206561699338266 2307 80-81 -0.09456627672669526 2307 82-83 -0.16591132918350127 2307 84-85 -0.1785600718987297 2307 86-87 -0.14356289007219658 2307 88-89 -0.2084762756548173 2307 90-91 -0.2255589687290538 2307 92-93 -0.29577999365521634 2307 94-95 -0.288393993143508 2307 96-97 -0.24264941139956875 2307 98-99 -0.490230845815816 2307 100-101 -0.0239331559074607 2201 1 0.17077075589715918 2201 2 0.16454530519018107 2201 3 0.12130375825915962 2201 4 0.15798476007094564 2201 5 0.16113901253478957 2201 6 0.09164195356554217 2201 7 0.09132505373818844 2201 8 0.08085276703246791 2201 9 0.034195091918881815 2201 10-11 0.07004619134902157 2201 12-13 0.10264442954061792 2201 14-15 0.13331454101254536 2201 16-17 0.13849695022701525 2201 18-19 0.12483728285522488 2201 20-21 0.0988895485901935 2201 22-23 0.12716552953320814 2201 24-25 0.1288485946984821 2201 26-27 0.0834267077881421 2201 28-29 0.16333792001093883 2201 30-31 0.11011939392354719 2201 32-33 0.07897392850163953 2201 34-35 0.11584240161867143 2201 36-37 0.10662711660111057 2201 38-39 0.1050900007392741 2201 40-41 0.0964950249116896 2201 42-43 0.08320171570206014 2201 44-45 0.12156715066434742 2201 46-47 0.11649934332647405 2201 48-49 0.03516363708892101 2201 50-51 0.010795041796306748 2201 52-53 0.09014660149790643 2201 54-55 0.1916493633803782 2201 56-57 0.09893090636212776 2201 58-59 0.09220578004695312 2201 60-61 0.038557162387533594 2201 62-63 0.06351351051893772 2201 64-65 0.02570448447676199 2201 66-67 0.020119965599377565 2201 68-69 0.041304279910328034 2201 70-71 0.04999750858186047 2201 72-73 -0.0074476740924680485 2201 74-75 0.038266611479294 2201 76-77 0.07439053090148917 2201 78-79 -0.07525051864817556 2201 80-81 -0.07970251665565797 2201 82-83 0.022327574618230983 2201 84-85 -0.0824634673099709 2201 86-87 -0.02066498957394458 2201 88-89 -0.0531700089054965 2201 90-91 -0.1497981314018375 2201 92-93 -0.14745052250717805 2201 94-95 -0.12676121363832493 2201 96-97 -0.1343168658229601 2201 98-99 -0.44011035086028727 2201 100-101 -0.0239331559074607 1101 1 0.08320152774494005 1101 2 0.07237231631866337 1101 3 0.07077361336754251 1101 4 0.13533633640282972 1101 5 0.09423654821792837 1101 6 0.11138440321258969 1101 7 0.07246330140858959 1101 8 0.06095729486348489 1101 9 0.04778247413970149 1101 10-11 0.028788266254451855 1101 12-13 -0.033516537067391994 1101 14-15 0.03098227423286204 1101 16-17 0.03328433433789968 1101 18-19 0.0070780923261963835 1101 20-21 -0.0032693411278188478 1101 22-23 0.012174011197224388 1101 24-25 0.0701266562484335 1101 26-27 0.09640436250173678 1101 28-29 0.044180081822986494 1101 30-31 -0.015386152870263459 1101 32-33 0.02311107593646966 1101 34-35 0.03427703574036656 1101 36-37 -0.05953607810206307 1101 38-39 -0.03189368909534096 1101 40-41 -0.03163643924487758 1101 42-43 -0.06992094329869758 1101 44-45 -0.014513851680426626 1101 46-47 -0.03842941760182583 1101 48-49 -0.12606267304374796 1101 50-51 -0.16404609283843996 1101 52-53 0.035024872263441864 1101 54-55 0.10858012414220752 1101 56-57 0.1262043539740887 1101 58-59 0.1182877601365746 1101 60-61 0.10286805762744677 1101 62-63 0.09183067350318908 1101 64-65 0.10434370353122802 1101 66-67 0.08168463360775036 1101 68-69 0.0015476838581633956 1101 70-71 0.0014224899265613544 1101 72-73 0.07353758740339345 1101 74-75 0.07516121385517494 1101 76-77 0.13320715430869967 1101 78-79 0.046179758511598834 1101 80-81 -0.008096699764372772 1101 82-83 -0.01312567191267533 1101 84-85 -0.1005982717918954 1101 86-87 -0.09472443718808421 1101 88-89 -0.22722567639082314 1101 90-91 -0.1898044340396723 1101 92-93 -0.1649499745702414 1101 94-95 -0.1865735738598815 1101 96-97 -0.19232421453875048 1101 98-99 -0.3347665208846635 1101 100-101 -0.0239331559074607 2304 1 0.09411384654345767 2304 2 0.060350320095395205 2304 3 0.09659188806266883 2304 4 0.09019935043787797 2304 5 0.11401362576774687 2304 6 0.09046434621548372 2304 7 0.040599884420203125 2304 8 0.08901230453698616 2304 9 0.0613202041188643 2304 10-11 0.03575035306046459 2304 12-13 0.09759663430020993 2304 14-15 0.07459174469277485 2304 16-17 0.07901485876885062 2304 18-19 0.08726253488845259 2304 20-21 0.08946118625915744 2304 22-23 0.07001827632357305 2304 24-25 0.08644243733026968 2304 26-27 0.0250204598210928 2304 28-29 0.04516127629182165 2304 30-31 -0.003950169375883661 2304 32-33 0.015169690932594904 2304 34-35 0.061976535584342685 2304 36-37 0.045704459744591475 2304 38-39 0.020822525204444275 2304 40-41 0.0189096639038695 2304 42-43 0.053196342937340546 2304 44-45 -8.827522577306013E-4 2304 46-47 -0.019330588983514474 2304 48-49 -0.06004933474721241 2304 50-51 0.00874475755635018 2304 52-53 0.14826448301163708 2304 54-55 0.15546249329918282 2304 56-57 0.05694183873686853 2304 58-59 0.05633049740434615 2304 60-61 0.09269861075340913 2304 62-63 0.08879106700423023 2304 64-65 0.027152449806671086 2304 66-67 0.04479369358858776 2304 68-69 0.018467129097338386 2304 70-71 0.04316563127977702 2304 72-73 0.058015956691299664 2304 74-75 -0.016168664489406126 2304 76-77 0.07561579973079091 2304 78-79 -0.009330830081815122 2304 80-81 0.05539211405404387 2304 82-83 -0.01416049043765355 2304 84-85 -0.014446374699396358 2304 86-87 0.004042316344744279 2304 88-89 0.04569839000701137 2304 90-91 0.010964094346263664 2304 92-93 -0.002192500183646473 2304 94-95 -0.02138087494511609 2304 96-97 0.023992546047587382 2304 98-99 -0.11448993956154752 2304 100-101 -0.0239331559074607 2202 1 0.07891676181954921 2202 2 0.06534006284921645 2202 3 0.07962218696474821 2202 4 0.0828626120135354 2202 5 0.09492577881563591 2202 6 0.09957458399507857 2202 7 0.12113026476153976 2202 8 0.070986668248878 2202 9 0.055865146019172585 2202 10-11 0.0361963137729191 2202 12-13 0.06398016672530815 2202 14-15 0.10850464168390062 2202 16-17 0.06692839081269852 2202 18-19 0.031129288856746484 2202 20-21 0.06864690600539802 2202 22-23 0.06133291019556708 2202 24-25 -0.0025230312601465243 2202 26-27 0.01080870332322803 2202 28-29 0.060224881057052926 2202 30-31 0.03728604787823997 2202 32-33 0.07599471296849458 2202 34-35 -0.030711676518343722 2202 36-37 -0.015220244566272356 2202 38-39 -0.002573442403139836 2202 40-41 0.09372168429328553 2202 42-43 -0.054597163259117565 2202 44-45 -0.014151003882027169 2202 46-47 0.004512592854524655 2202 48-49 -0.028117800355708766 2202 50-51 -0.1889985442189257 2202 52-53 -0.018216804897228656 2202 54-55 0.16934102686791164 2202 56-57 0.10084392614551518 2202 58-59 0.07808426181353667 2202 60-61 0.10140836923817176 2202 62-63 0.05026240134939286 2202 64-65 0.09192175692466975 2202 66-67 0.11516518555536948 2202 68-69 0.014071277948673355 2202 70-71 0.06009695422881123 2202 72-73 0.06847524789765913 2202 74-75 -0.027716915961072175 2202 76-77 -0.05005732761500781 2202 78-79 0.07223256735627359 2202 80-81 0.08772665054391382 2202 82-83 0.0489765706721812 2202 84-85 -0.01331451889009827 2202 86-87 0.034324087010993765 2202 88-89 -0.005953182253037426 2202 90-91 -0.06242316485883492 2202 92-93 -0.05187127949235304 2202 94-95 -0.06506515768667498 2202 96-97 0.026822004244309028 2202 98-99 -0.10819929739357548 2202 100-101 -0.0239331559074607 1102 1 -0.03729014936260455 1102 2 -0.04239588561660668 1102 3 -0.022888868416288233 1102 4 -0.017322343388734396 1102 5 -0.02438624495264463 1102 6 -0.0905534037672382 1102 7 -0.08215916812628166 1102 8 -0.10828471613896085 1102 9 -0.058053462489567664 1102 10-11 -0.05994823317853104 1102 12-13 -0.03217401699534861 1102 14-15 -0.11183860900961662 1102 16-17 -0.11042869457666171 1102 18-19 -0.17071273216738092 1102 20-21 -0.19679911498803904 1102 22-23 -0.1219475024998502 1102 24-25 -0.040906834360612265 1102 26-27 -0.051288167686173836 1102 28-29 -0.012724072645617923 1102 30-31 -0.05060618296282371 1102 32-33 -0.05611716758460261 1102 34-35 -0.08158486483880978 1102 36-37 -0.09781769920663663 1102 38-39 -0.03261522143630202 1102 40-41 -0.08226009961073544 1102 42-43 -0.10230475007361406 1102 44-45 -0.07203307840632078 1102 46-47 -0.12818097808405327 1102 48-49 -0.0743937866647073 1102 50-51 -0.019548108217961158 1102 52-53 0.032901999751736355 1102 54-55 0.07339997672733745 1102 56-57 0.02224094542442856 1102 58-59 1.8439035965656103E-4 1102 60-61 -0.05418011451829585 1102 62-63 -0.010237765055769898 1102 64-65 0.022941321815494575 1102 66-67 0.06629887096376308 1102 68-69 -0.03949409732035036 1102 70-71 -0.013392739103174733 1102 72-73 0.03651998579385918 1102 74-75 0.025162153743600868 1102 76-77 0.06858492966653529 1102 78-79 0.04140957050540095 1102 80-81 -0.011132929335289532 1102 82-83 -0.012811240107005517 1102 84-85 -0.03570384313162123 1102 86-87 -0.010013453246223492 1102 88-89 -0.012778771372779829 1102 90-91 -0.046208231121440235 1102 92-93 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-0.5519582509861678 1213 88-89 -0.49860248145524366 1213 90-91 -0.4624442662253969 1213 92-93 -0.4887462233749531 1213 94-95 -0.4772902038222142 1213 96-97 -0.557144391493047 1213 98-99 -0.13159117753879457 1213 100-101 -0.0239331559074607 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 104.0 3 80.0 4 191.0 5 235.0 6 1805.0 7 14486.0 8 26626.0 9 26634.0 10 19710.0 11 14192.0 12 12330.0 13 12307.0 14 13339.0 15 14979.0 16 17053.0 17 19883.0 18 23504.0 19 27628.0 20 32469.0 21 37806.0 22 44746.0 23 52609.0 24 62899.0 25 77058.0 26 97336.0 27 130786.0 28 152425.0 29 178756.0 30 236100.0 31 318712.0 32 452770.0 33 680714.0 34 1172266.0 35 2554932.0 36 5709591.0 37 3336006.0 38 25389.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.922153772260753 24.463070549846787 30.077537350262055 24.537238327630405 2 26.141044809519055 24.833567776223376 28.591544744181107 20.433842670076466 3 21.872282389809 22.721568442347287 33.430979178520474 21.97516998932324 4 21.608593857595373 23.322105265317607 32.529012040294866 22.540288836792158 5 26.097819564977247 22.58891806188312 30.086270726455 21.226991646684628 6 27.537459514885477 23.191853035779047 28.659311066408094 20.611376382927382 7 26.11780771075462 27.083148245210648 25.564535077986537 21.234508966048192 8 25.107658761556724 24.417520239975453 28.63513320053816 21.83968779792967 9 22.812414225135978 23.303276570138255 31.362372488718854 22.52193671600692 10-11 25.572486580580396 24.142994556342927 27.672071534485067 22.612447328591614 12-13 25.043113089528855 26.148099625439546 25.748018238165688 23.06076904686591 14-15 24.689073322796798 25.065504760847716 26.809678684881376 23.435743231474106 16-17 24.361666603426414 25.031427413171958 26.52537500001203 24.0815309833896 18-19 24.288738281272554 25.200452549939918 26.819858307088555 23.690950861698965 20-21 23.764734487329015 25.478496341317896 27.061532322528574 23.695236848824514 22-23 23.91503590719271 25.70665531038892 26.544262056972528 23.83404672544584 24-25 24.215631099119346 25.601275633642818 26.288324061241834 23.894769205996006 26-27 24.098133915538263 25.62717566230446 26.50695292939979 23.767737492757483 28-29 24.06550760464043 25.965363762801402 26.223837686921094 23.745290945637073 30-31 24.157107155358336 25.849714822709895 26.125016044400574 23.868161977531198 32-33 24.080726262655524 26.15854240088249 26.02744981908845 23.733281517373538 34-35 23.93208701601732 26.11537057847315 26.126503854535887 23.826038550973642 36-37 24.046770505369622 25.92600768114265 26.181612633128847 23.845609180358878 38-39 24.127152669135317 25.839712126297513 26.180845403243293 23.852289801323874 40-41 23.848699925915398 26.065767491137283 26.042679019875788 24.042853563071535 42-43 23.89178845859358 25.91613004298754 26.043927708351696 24.14815379006719 44-45 24.09069340112577 25.846501298898257 26.080121033898173 23.9826842660778 46-47 23.89414618290078 25.933123070095697 26.12725022469758 24.045480522305947 48-49 23.918653115035056 25.703228458066985 26.07740310396423 24.30071532293373 50-51 23.891008389797 25.846438477543792 26.301553033101893 23.96100009955731 52-53 23.923282161411223 25.88088765994247 26.340890121657225 23.854940056989083 54-55 23.82812275625737 25.92689965730253 26.029344683953482 24.215632902486615 56-57 23.925259619132756 26.058156983060744 26.164324739666966 23.852258658139537 58-59 23.797919452374323 26.245663119409702 26.15521568696654 23.801201741249436 60-61 24.15467902967387 26.00396409678695 25.847979381383094 23.993377492156085 62-63 23.890050245355383 26.16854981807495 26.217840670519866 23.723559266049808 64-65 24.023703578409492 26.091389981449314 26.09871798174808 23.786188458393113 66-67 24.114076312553863 26.103939113406717 26.146784369521818 23.6352002045176 68-69 24.051494865484006 25.932714646580123 26.373555032361594 23.64223545557427 70-71 23.94121414238512 26.1758358675292 26.193550105207365 23.68939988487831 72-73 23.86993208249329 25.940846713194553 26.289136930252155 23.90008427406 74-75 23.966483391620034 25.96418590501169 26.239767808272564 23.82956289509571 76-77 23.99437295336611 25.982408400413533 26.073767466673875 23.949451179546482 78-79 23.92384636945523 25.927219848122334 26.065750102723772 24.083183679698664 80-81 23.946078239632886 25.923366556523643 26.182709550623347 23.947845653220128 82-83 23.757262962141237 26.344456816126517 26.1711037539163 23.72717646781595 84-85 23.915502080480703 26.152549101423777 25.970195236694067 23.961753581401453 86-87 23.852170570834172 26.22230144910655 26.066642072725454 23.85888590733383 88-89 23.94105541295398 26.809628151243338 25.6878576876384 23.561458748164288 90-91 23.967769943341693 26.24007670477057 25.79639319379643 23.995760158091308 92-93 23.879800626351187 26.39444963180768 25.749683923709753 23.976065818131378 94-95 23.993731604738404 26.598321404121567 25.594477767489494 23.813469223650532 96-97 24.00371515916382 26.303463920753305 25.657303267591992 24.03551765249088 98-99 24.37301548899818 26.197481960043785 25.58598490298175 23.843517647976284 100-101 32.15684926799093 49.78091277751048 5.890439205443673 12.171798749054918 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2122.0 1 1654.5 2 1290.5 3 1409.5 4 1482.0 5 1501.0 6 1526.0 7 1688.5 8 1919.5 9 2110.0 10 2419.5 11 2845.0 12 3271.5 13 3806.0 14 4333.0 15 4801.0 16 5209.0 17 5554.0 18 5972.0 19 6560.0 20 7353.0 21 8295.5 22 9450.0 23 11101.0 24 13485.5 25 16810.0 26 20968.5 27 25864.5 28 31904.5 29 40251.0 30 51764.0 31 67136.0 32 87143.5 33 112156.5 34 143291.5 35 180462.0 36 221105.0 37 264551.0 38 306930.0 39 355859.5 40 411093.0 41 470146.5 42 517797.5 43 551853.5 44 623759.5 45 735397.5 46 835126.0 47 911034.5 48 966681.5 49 956430.5 50 910872.0 51 853087.0 52 780913.0 53 711740.0 54 645849.0 55 602412.0 56 531030.0 57 409536.0 58 302567.5 59 231311.5 60 177772.5 61 133275.5 62 94915.5 63 65065.5 64 41597.0 65 25843.0 66 16588.0 67 11954.0 68 8969.5 69 7036.0 70 5365.5 71 3968.0 72 2977.0 73 2159.5 74 1545.5 75 1099.0 76 798.0 77 565.0 78 418.5 79 338.5 80 266.0 81 191.0 82 148.5 83 126.5 84 108.5 85 86.5 86 60.0 87 52.0 88 54.0 89 46.0 90 32.0 91 27.5 92 24.5 93 16.5 94 13.0 95 10.0 96 5.5 97 2.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 2.4361385511489084E-4 2 0.016783712439231165 3 0.03661900895832254 4 0.011719108609211066 5 0.004468390974081024 6 0.052300048158612625 7 0.05792239949902733 8 0.0482611868764447 9 0.0573967064432531 10-11 0.06376592657632268 12-13 0.07213855012316604 14-15 0.07321878524387286 16-17 0.06254785730074822 18-19 0.07196866151367802 20-21 0.06972484969025139 22-23 0.0717314585494872 24-25 0.06993320364528387 26-27 0.06934981257119294 28-29 0.07178274567687981 30-31 0.0820626092736358 32-33 0.0802932033785908 34-35 0.0932367921543004 36-37 0.09261493573466502 38-39 0.11280283125458057 40-41 0.09565690347813911 42-43 0.09010827738335128 44-45 0.11133473723296716 46-47 0.10514502204577172 48-49 0.10874153185417838 50-51 0.09274956444407062 52-53 0.10143311620073167 54-55 0.10852676700822185 56-57 0.0968012475080867 58-59 0.09499978715842132 60-61 0.10154530679190299 62-63 0.09249953969803165 64-65 0.09228798029753714 66-67 0.09274635899860859 68-69 0.11458505893147373 70-71 0.09581717575124102 72-73 0.09092246053070893 74-75 0.3885448662354787 76-77 0.37965937141470923 78-79 0.40299180893288417 80-81 0.4313664121628449 82-83 0.4267730088157443 84-85 0.40917511322915545 86-87 0.38079730455373273 88-89 0.36854288655236134 90-91 0.3674113643042619 92-93 0.3788227501491173 94-95 0.3899552622387754 96-97 0.35676287447937155 98-99 0.3644206836881804 100-101 99.62691179178248 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5598456E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.6046492391962 #Duplication Level Percentage of deduplicated Percentage of total 1 82.99662290575654 30.380622695030553 2 8.903047993507203 6.517858979241215 3 2.38567626113516 2.6198052821138873 4 1.1522963891132922 1.6871762057233766 5 0.6997377001104002 1.2806826535991531 6 0.5669176766869115 1.245109362157466 7 0.4954069950279986 1.2693939498550875 8 0.25770405998347395 0.7546533378569474 9 0.23640568932564923 0.7788192602324202 >10 1.6740709567341066 12.625900766284005 >50 0.2876107124029776 7.65922711369608 >100 0.31442158257600245 22.522218546234996 >500 0.020293361183862615 5.156494314221816 >1k 0.009768989656601121 5.46704337445982 >5k 1.872679982984455E-5 0.03499415929330625 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.616336386114112E-5 0.0 0.0 2 9.616336386114112E-5 0.0 0.0 3 9.616336386114112E-5 6.410890924076075E-6 0.0 4 1.025742547852172E-4 6.410890924076075E-6 0.0 5 1.025742547852172E-4 6.410890924076075E-6 0.0 6 1.2180692755744544E-4 6.410890924076075E-6 0.0 7 1.4103960032967364E-4 6.410890924076075E-6 0.0 8 1.602722731019019E-4 6.410890924076075E-6 0.0 9 1.795049458741301E-4 6.410890924076075E-6 6.410890924076075E-6 10-11 2.1797029141858656E-4 6.410890924076075E-6 6.410890924076075E-6 12-13 3.590098917482602E-4 6.410890924076075E-6 6.410890924076075E-6 14-15 4.744059283816296E-4 6.410890924076075E-6 6.410890924076075E-6 16-17 6.314727560214933E-4 6.410890924076075E-6 6.410890924076075E-6 18-19 8.302103746678517E-4 6.410890924076075E-6 6.410890924076075E-6 20-21 0.00102894799331421 6.410890924076075E-6 6.410890924076075E-6 22-23 0.001278972739353177 6.410890924076075E-6 6.410890924076075E-6 24-25 0.0015578464945504864 9.616336386114112E-6 6.410890924076075E-6 26-27 0.001868774704368176 1.282178184815215E-5 6.410890924076075E-6 28-29 0.002208551923344208 1.282178184815215E-5 9.616336386114112E-6 30-31 0.0026092326060989626 1.282178184815215E-5 1.282178184815215E-5 32-33 0.003093254870866706 1.282178184815215E-5 1.282178184815215E-5 34-35 0.0038016583179771124 1.282178184815215E-5 1.282178184815215E-5 36-37 0.004484418201391215 1.282178184815215E-5 1.282178184815215E-5 38-39 0.005465284512774855 1.9232672772228225E-5 1.282178184815215E-5 40-41 0.006551930524405749 1.9232672772228225E-5 1.282178184815215E-5 42-43 0.007968737418626562 1.9232672772228225E-5 1.282178184815215E-5 44-45 0.009709294304513217 1.9232672772228225E-5 1.282178184815215E-5 46-47 0.011924257118781499 1.9232672772228225E-5 1.282178184815215E-5 48-49 0.014764281798147202 1.9232672772228225E-5 1.282178184815215E-5 50-51 0.017956905478337087 1.9232672772228225E-5 1.282178184815215E-5 52-53 0.021825878151016997 2.243811823426626E-5 1.282178184815215E-5 54-55 0.02683598940818245 2.56435636963043E-5 1.282178184815215E-5 56-57 0.03282376153126951 2.56435636963043E-5 1.282178184815215E-5 58-59 0.04013538263017827 2.56435636963043E-5 1.602722731019019E-5 60-61 0.04908819180565051 3.205445462038038E-5 1.9232672772228225E-5 62-63 0.060505988541429995 3.846534554445646E-5 1.9232672772228225E-5 64-65 0.07485035698405021 4.487623646853253E-5 1.9232672772228225E-5 66-67 0.09324640849068652 4.487623646853253E-5 1.9232672772228225E-5 68-69 0.11636408116290484 4.808168193057057E-5 1.9232672772228225E-5 70-71 0.14536054081250094 5.12871273926086E-5 1.9232672772228225E-5 72-73 0.18174234680663265 5.12871273926086E-5 1.9232672772228225E-5 74-75 0.22783985799620166 5.12871273926086E-5 1.9232672772228225E-5 76-77 0.2846243243562055 5.12871273926086E-5 1.9232672772228225E-5 78-79 0.35447739186493843 5.449257285464664E-5 1.9232672772228225E-5 80-81 0.4403063995564689 6.410890924076076E-5 1.9232672772228225E-5 82-83 0.5452687112109045 7.051980016483682E-5 1.9232672772228225E-5 84-85 0.6751661831145339 7.69306910889129E-5 1.9232672772228225E-5 86-87 0.8325407335187534 7.69306910889129E-5 1.9232672772228225E-5 88-89 0.9718654205262367 7.69306910889129E-5 1.9232672772228225E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCGA 1755 2.355995E-6 141.99232 94-95 CGCACTA 2180 9.92785E-4 85.73274 94-95 CTTTGTG 76765 0.0 65.38319 1 TTTGTGT 85050 0.0 59.124157 2 TCTCGCA 4400 2.2528776E-4 56.635574 94-95 TTGTGTT 95980 0.0 53.827526 3 CTCCAGA 3610 0.0073300856 51.77213 94-95 ACTTTGT 23785 0.0 49.83124 1 TGTGTTT 104950 0.0 49.536858 4 GTGTTTG 115860 0.0 44.836697 5 AGTTCTA 6980 4.0640967E-5 44.62689 94-95 GTTTGAG 95785 0.0 34.18399 2 TGTTTGA 187075 0.0 33.1056 1 AACTTTG 11325 0.0 30.388632 2 TAACTTT 11740 0.0 29.590868 1 TTTGAGT 41300 0.0 29.384178 3 TTTGAGG 36260 0.0 28.965002 8 TGACCGG 2435 4.8619768E-8 25.584858 94-95 TTGAGGG 19400 0.0 25.524035 9 GGCTCAG 4915 0.0 25.350615 94-95 >>END_MODULE