FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l03n02_6929.34200000035b94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l03n02_6929.34200000035b94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15598456
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA17552.355995E-6141.9923294-95
CGCACTA21809.92785E-485.7327494-95
CTTTGTG767650.065.383191
TTTGTGT850500.059.1241572
TCTCGCA44002.2528776E-456.63557494-95
TTGTGTT959800.053.8275263
CTCCAGA36100.007330085651.7721394-95
ACTTTGT237850.049.831241
TGTGTTT1049500.049.5368584
GTGTTTG1158600.044.8366975
AGTTCTA69804.0640967E-544.6268994-95
GTTTGAG957850.034.183992
TGTTTGA1870750.033.10561
AACTTTG113250.030.3886322
TAACTTT117400.029.5908681
TTTGAGT413000.029.3841783
TTTGAGG362600.028.9650028
TGACCGG24354.8619768E-825.58485894-95
TTGAGGG194000.025.5240359
GGCTCAG49150.025.35061594-95
Produced by FastQC (version 0.11.2)