FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l03n02_6777.34200000035b44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l03n02_6777.34200000035b44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12714603
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCTG35258.223007E-569.421594-95
CCATCCG26450.002297247869.3886994-95
CTTTGTG1084750.067.934851
TTTGTGT1220400.060.430132
CAAATTA30950.00428155559.29986294-95
TTGTGTT1336800.056.5022543
GATCGGT22100.055.3644394-95
TGTGTTT1439800.052.716934
GTGTTTG1525000.049.728865
AACTTTG128250.049.6885952
ACTTTGT280500.049.1788561
TAACTTT135800.046.5835081
GAGCCGG14103.2732714E-843.38843594-95
TTTGAGG425200.035.2776578
GTTTGAG1172600.033.8459937
TGTTTGA2244600.033.7717746
TTGAGGG198450.033.731279
GGACGCG18750.032.62810594-95
AGTCCCC19250.031.78061794-95
TGACCCA40500.030.21120694-95
Produced by FastQC (version 0.11.2)