FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l03n02_30082.34200000035d35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l03n02_30082.34200000035d35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15022337
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGAGTAGCGAAAGACAGGTGAGAATCCTGTCCACCGTAAGACTAAG164460.10947697418850343No Hit
CTTTGTGTTTGAGGGGTCCATCCCGGATTACCAATCTCAGATAAACTCCG161480.10749326153447364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTG8802.7123695E-5211.7472494-95
ACATCCG14007.760864E-7177.4643694-95
GGAGTTA11257.222058E-5165.633494-95
CAGTCTA7550.0048533166164.536594-95
CGCCTAA8600.0071638026144.4477494-95
TCCTAGA12951.2652646E-4143.8900194-95
TCTCGCG9150.008622311135.7650994-95
CGGAAGG20658.1000343E-490.23611594-95
CTTTGTG1586550.077.191141
GATCCAC16552.4975965E-575.06045594-95
TTTGTGT1695650.072.244472
GGCCCTA8652.4627116E-771.8063994-95
GATCCCG17650.070.3824794-95
TATATCG9202.662538E-567.5136194-95
GAATCGG9350.066.4305194-95
GCGCCTA9352.9458533E-866.4305194-95
TTGTGTT1912300.065.9436343
CGTTTAG19200.064.7005594-95
TGTGTTT1981700.063.8982244
GTTCTCA41051.6222801E-460.52377794-95
Produced by FastQC (version 0.11.2)