FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l03n02_30076.34200000035ce3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l03n02_30076.34200000035ce3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16090946
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT228800.1421917642381001No Hit
CTTTGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC215530.13394489049929073No Hit
TGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT175400.10900539968252954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGA7704.0252417E-8321.284794-95
TCCCACG3957.0709956E-4313.150994-95
GCGTCGG5350.0017539391231.2048894-95
GTCGTGC5800.0022335749213.2665994-95
ATCGTGA6700.0034392823184.6188294-95
CACGATG7000.003920847176.7065994-95
TATTGTA7200.0042655803171.7980794-95
CACGATA7550.004916289163.8339294-95
ATATTAG15251.2141772E-6162.2224394-95
GCATTGG8400.006763752147.2554894-95
TCAATTA13151.3682853E-4141.0965194-95
CCACTAA13351.4530706E-4138.982794-95
TATTAAG9300.009169076133.0049694-95
AGAGGCA15352.5332283E-4120.87421494-95
ATATTAA17103.892643E-4108.5040594-95
TTGGACG11951.5643309E-10103.5101494-95
GAGCGTA21209.147588E-487.51977594-95
TCTTAGA21559.762391E-486.09833594-95
AAAGATA21750.001012726285.3066394-95
GCTAGCT7400.083.5774494-95
Produced by FastQC (version 0.11.2)