##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l03n01_8033.34100000035d6e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17575583 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74072672297699 33.0 33.0 33.0 33.0 33.0 2 32.7530893854275 33.0 33.0 33.0 33.0 33.0 3 32.78335455500964 33.0 33.0 33.0 33.0 33.0 4 36.47004033948689 37.0 37.0 37.0 37.0 37.0 5 36.578380074220014 37.0 37.0 37.0 37.0 37.0 6 36.61945256666593 37.0 37.0 37.0 37.0 37.0 7 36.607899095011526 37.0 37.0 37.0 37.0 37.0 8 36.61440704413617 37.0 37.0 37.0 37.0 37.0 9 36.6071748516109 37.0 37.0 37.0 37.0 37.0 10-11 36.5887509961974 37.0 37.0 37.0 37.0 37.0 12-13 36.55397707148605 37.0 37.0 37.0 37.0 37.0 14-15 38.64198026887643 40.0 37.0 40.0 37.0 40.0 16-17 38.60138483030691 40.0 37.0 40.0 37.0 40.0 18-19 38.57177380687742 40.0 37.0 40.0 37.0 40.0 20-21 38.52554814255664 40.0 37.0 40.0 37.0 40.0 22-23 38.463960256681105 40.0 37.0 40.0 37.0 40.0 24-25 38.413422416769905 40.0 37.0 40.0 37.0 40.0 26-27 38.32099222540726 40.0 37.0 40.0 37.0 40.0 28-29 38.25517364061266 40.0 37.0 40.0 37.0 40.0 30-31 38.195447741335244 40.0 37.0 40.0 37.0 40.0 32-33 38.095172205667374 40.0 37.0 40.0 37.0 40.0 34-35 38.02230014219158 40.0 37.0 40.0 37.0 40.0 36-37 37.915880372218666 40.0 37.0 40.0 35.0 40.0 38-39 37.80109171911964 40.0 37.0 40.0 33.0 40.0 40-41 37.67460359636434 40.0 37.0 40.0 33.0 40.0 42-43 37.547156700292675 40.0 37.0 40.0 33.0 40.0 44-45 37.405372726469444 40.0 37.0 40.0 33.0 40.0 46-47 37.23962892155555 38.5 37.0 40.0 33.0 40.0 48-49 37.02899690439857 37.0 37.0 40.0 33.0 40.0 50-51 36.83759420669004 37.0 37.0 40.0 33.0 40.0 52-53 36.61444465313043 37.0 37.0 40.0 33.0 40.0 54-55 36.38089561524076 37.0 37.0 40.0 33.0 40.0 56-57 36.133752035423235 37.0 37.0 40.0 33.0 40.0 58-59 35.86333286924251 37.0 37.0 40.0 33.0 40.0 60-61 35.57406815466662 37.0 33.0 38.5 33.0 40.0 62-63 35.314330511824274 37.0 33.0 37.0 33.0 40.0 64-65 35.03325844155496 37.0 33.0 37.0 27.0 40.0 66-67 34.780073013794194 37.0 33.0 37.0 27.0 40.0 68-69 34.49168605104024 37.0 33.0 37.0 27.0 37.0 70-71 34.227481131066895 37.0 33.0 37.0 27.0 37.0 72-73 33.98511969702513 37.0 33.0 37.0 27.0 37.0 74-75 33.72870168801798 37.0 33.0 37.0 27.0 37.0 76-77 30.87335114288954 33.0 30.0 35.0 24.5 37.0 78-79 33.46980916650105 35.0 33.0 37.0 27.0 37.0 80-81 33.93971426154114 37.0 33.0 37.0 27.0 37.0 82-83 33.91504907689264 37.0 33.0 37.0 27.0 37.0 84-85 33.815156117438605 37.0 33.0 37.0 27.0 37.0 86-87 33.718884431884845 37.0 33.0 37.0 27.0 37.0 88-89 33.569859076651966 37.0 33.0 37.0 27.0 37.0 90-91 33.451452591928245 37.0 33.0 37.0 27.0 37.0 92-93 33.32235897950014 37.0 33.0 37.0 27.0 37.0 94-95 33.20298384981028 37.0 33.0 37.0 27.0 37.0 96-97 33.105511066119405 37.0 33.0 37.0 27.0 37.0 98-99 32.95068288773123 33.0 33.0 37.0 27.0 37.0 100-101 31.015486342615205 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.06858158535786885 2306 2 0.08352021680252619 2306 3 0.0656705491136691 2306 4 -0.12051781610322365 2306 5 -0.06694463184907562 2306 6 0.07218942397271633 2306 7 0.09468398469697092 2306 8 0.07664303429061903 2306 9 0.0735827363177961 2306 10-11 0.0918912839397521 2306 12-13 0.11115055364527393 2306 14-15 0.19604574232621275 2306 16-17 0.15481987271139275 2306 18-19 0.14599033074259893 2306 20-21 0.13024371615441765 2306 22-23 0.15944691333397998 2306 24-25 0.1349531851911152 2306 26-27 0.14307219732570076 2306 28-29 0.12511153474982706 2306 30-31 0.10362651803117728 2306 32-33 0.1367291606935055 2306 34-35 0.130624137264725 2306 36-37 0.14836669717886508 2306 38-39 0.05923620442713684 2306 40-41 0.010064654656780192 2306 42-43 0.04893391441827788 2306 44-45 0.032984128229458065 2306 46-47 -0.0396200295114042 2306 48-49 -0.006059078484000224 2306 50-51 -0.019743605208205395 2306 52-53 0.01653268860501811 2306 54-55 0.057002838449221827 2306 56-57 -0.07498349465949161 2306 58-59 -0.1917058218130876 2306 60-61 -0.10669970837166431 2306 62-63 -0.05717753161655992 2306 64-65 -0.11166678728935153 2306 66-67 -0.14687628665748065 2306 68-69 -0.22309281210137755 2306 70-71 -0.21504254055582805 2306 72-73 -0.3073958435134827 2306 74-75 -0.33604014009995353 2306 76-77 -0.6080539282388422 2306 78-79 -0.2590469342729662 2306 80-81 -0.10412419335627021 2306 82-83 -0.13216194448452967 2306 84-85 -0.283885676968616 2306 86-87 -0.3170480439468264 2306 88-89 -0.297473892211805 2306 90-91 -0.3473763432807999 2306 92-93 -0.4257637590760268 2306 94-95 -0.41852200801867667 2306 96-97 -0.5391324471617978 2306 98-99 -0.5453016867740317 2306 100-101 -0.8739151472856292 2307 1 0.09588196715082375 2307 2 0.09279245953905502 2307 3 0.07363865505907796 2307 4 -0.08171541932536286 2307 5 -0.016853745987567947 2307 6 0.10977111167592568 2307 7 0.12714633421908417 2307 8 0.12732225761644855 2307 9 0.07818068233422082 2307 10-11 0.0824745323835927 2307 12-13 0.09507571333820408 2307 14-15 0.1333941203659137 2307 16-17 0.1258438406683169 2307 18-19 0.0867949246466182 2307 20-21 0.12801872494139133 2307 22-23 0.12491308165001413 2307 24-25 0.07443420753033791 2307 26-27 0.011053983839140358 2307 28-29 -0.037195264354075164 2307 30-31 0.010830397893769828 2307 32-33 -0.0305511793233606 2307 34-35 0.07451025432060021 2307 36-37 0.018007559686509467 2307 38-39 -0.02354070507800543 2307 40-41 -0.041227089528611316 2307 42-43 -0.09273820078291095 2307 44-45 -0.0567291235834233 2307 46-47 -0.0768991398027552 2307 48-49 -0.09681072255646228 2307 50-51 -0.16269468788554775 2307 52-53 -0.18592362206426571 2307 54-55 -0.1347798215826046 2307 56-57 -0.18919931499313236 2307 58-59 -0.22706590554528105 2307 60-61 -0.30702216192511145 2307 62-63 -0.27772186043372216 2307 64-65 -0.2548243026482666 2307 66-67 -0.294788992774329 2307 68-69 -0.39482273477410246 2307 70-71 -0.4619671692729099 2307 72-73 -0.49466113283102686 2307 74-75 -0.5432504449177529 2307 76-77 -0.7478258411946719 2307 78-79 -0.2812465590951234 2307 80-81 -0.16804252227323246 2307 82-83 -0.20270119162834277 2307 84-85 -0.33437781324860083 2307 86-87 -0.3194171090943456 2307 88-89 -0.3902793907417319 2307 90-91 -0.4292476625622399 2307 92-93 -0.4701191474479245 2307 94-95 -0.5431785885447269 2307 96-97 -0.5426166307323967 2307 98-99 -0.6545168574541051 2307 100-101 -0.9627881875074991 2201 1 0.05661214973714834 2201 2 0.0642123631048932 2201 3 0.08260856012084616 2201 4 -0.07264837196709806 2201 5 -0.0417492828989765 2201 6 0.06352199814406845 2201 7 0.07175386630034097 2201 8 0.0456385466298741 2201 9 0.06261379673880896 2201 10-11 0.047505886138559106 2201 12-13 0.0481643701017731 2201 14-15 0.111384889889095 2201 16-17 0.014893614894681662 2201 18-19 0.06518377215308391 2201 20-21 0.01344791066438944 2201 22-23 0.013184496491284392 2201 24-25 0.004623401933443461 2201 26-27 0.0868327644108362 2201 28-29 0.07137124917722559 2201 30-31 0.02656223859079887 2201 32-33 0.025816389705092035 2201 34-35 -0.0037068693915145445 2201 36-37 -0.01627933071360843 2201 38-39 -0.0049788302889837155 2201 40-41 0.014541906120548731 2201 42-43 -0.0525279665859415 2201 44-45 -0.14691831405915678 2201 46-47 -0.0730266600607763 2201 48-49 -0.12096395144041594 2201 50-51 -0.1786753307837614 2201 52-53 -0.1517558798906009 2201 54-55 -0.12503487023537474 2201 56-57 -0.23526457918880794 2201 58-59 -0.17482964335567885 2201 60-61 -0.21562598861157056 2201 62-63 -0.28107360197964226 2201 64-65 -0.23815976398515204 2201 66-67 -0.3509059721390315 2201 68-69 -0.4732914375482764 2201 70-71 -0.5217687509587563 2201 72-73 -0.480207745781307 2201 74-75 -0.5834349421713512 2201 76-77 -0.9987464095908543 2201 78-79 -0.49144005730528306 2201 80-81 -0.28854420783029155 2201 82-83 -0.2597619663323485 2201 84-85 -0.27765400544862473 2201 86-87 -0.42446611784363597 2201 88-89 -0.3806024611565064 2201 90-91 -0.3801715108263437 2201 92-93 -0.3724513394805413 2201 94-95 -0.561273260900272 2201 96-97 -0.5378933087395694 2201 98-99 -0.6890047543134656 2201 100-101 -0.8944833266737433 1101 1 0.09047980921772592 1101 2 0.08643371394458654 1101 3 0.0750605602951282 1101 4 -0.09097623573534008 1101 5 -0.07261391455948996 1101 6 0.05414112480326594 1101 7 0.01270457275264647 1101 8 0.042021775045938625 1101 9 0.07322805115158815 1101 10-11 0.04648689796358951 1101 12-13 0.03739632604167298 1101 14-15 0.07322013862430055 1101 16-17 0.051214253683660615 1101 18-19 0.05716181518720731 1101 20-21 0.06099581679305999 1101 22-23 0.07725496322905911 1101 24-25 0.09742839636081868 1101 26-27 0.06357316128341495 1101 28-29 0.06494531833221373 1101 30-31 0.0656170134157179 1101 32-33 0.0027192197775391946 1101 34-35 0.04560666388194079 1101 36-37 -0.08510247238495339 1101 38-39 -0.05740706889225322 1101 40-41 -0.057948931479522514 1101 42-43 -0.06069759009715625 1101 44-45 -0.09662943669499668 1101 46-47 -0.09281534511173817 1101 48-49 -0.07942923598194795 1101 50-51 -0.08571175878999782 1101 52-53 -0.06833132311276557 1101 54-55 -0.08825546728233036 1101 56-57 -0.18224609604092734 1101 58-59 -0.19680580776401513 1101 60-61 -0.22637933160506662 1101 62-63 -0.31199085858634135 1101 64-65 -0.2972303173927031 1101 66-67 -0.37718114912103573 1101 68-69 -0.2997316243989232 1101 70-71 -0.294948791239122 1101 72-73 -0.32655423090057667 1101 74-75 -0.4024339204050591 1101 76-77 -0.634651417651142 1101 78-79 -0.27920059951135556 1101 80-81 -0.11931167565355594 1101 82-83 -0.118057495249829 1101 84-85 -0.1543410605926141 1101 86-87 -0.2090923907765756 1101 88-89 -0.24690303472297614 1101 90-91 -0.2286362238158972 1101 92-93 -0.27633570982936106 1101 94-95 -0.5082843298008584 1101 96-97 -0.47650270870836664 1101 98-99 -0.5261692989762707 1101 100-101 -0.7759623064440859 2304 1 0.06703137135068005 2304 2 0.08913632141463523 2304 3 0.07452614638862087 2304 4 -0.10269536501260745 2304 5 -0.07750859782626662 2304 6 0.012976376545495327 2304 7 0.039251436741366774 2304 8 0.02090831486270872 2304 9 0.052428192095909765 2304 10-11 0.030219965068752686 2304 12-13 0.036931601274623915 2304 14-15 0.04101044478669991 2304 16-17 0.06096740535542011 2304 18-19 0.008703002251643 2304 20-21 0.048335101109088896 2304 22-23 0.055181319818210284 2304 24-25 0.044415124103352355 2304 26-27 0.07708678590169171 2304 28-29 -0.011571790743040822 2304 30-31 0.017600217550082675 2304 32-33 0.025390897798054368 2304 34-35 -0.0066496198859624656 2304 36-37 -0.025746705412899473 2304 38-39 -0.042830684897268156 2304 40-41 -0.025871960216498735 2304 42-43 -0.049951955456172925 2304 44-45 -0.03402901386201762 2304 46-47 -0.052964902703159566 2304 48-49 -0.04973931350133398 2304 50-51 -0.08730560422105782 2304 52-53 0.0030644172364659994 2304 54-55 -0.08371921326932608 2304 56-57 -0.052520937478554686 2304 58-59 -0.12485118400706341 2304 60-61 -0.04962770683018647 2304 62-63 -0.15611935283111222 2304 64-65 -0.22317386135950557 2304 66-67 -0.26225690669679125 2304 68-69 -0.26393744226753313 2304 70-71 -0.2660434588454166 2304 72-73 -0.2347969292014156 2304 74-75 -0.30173683325448764 2304 76-77 -0.5661007951470296 2304 78-79 -0.15295069738935752 2304 80-81 -0.05389960925311499 2304 82-83 -0.06628727365402654 2304 84-85 -0.11200177627658547 2304 86-87 -0.16439679137167218 2304 88-89 -0.22231001146716522 2304 90-91 -0.3161769410659119 2304 92-93 -0.2876036215042461 2304 94-95 -0.2890188063492829 2304 96-97 -0.2534677075338294 2304 98-99 -0.4432456829712592 2304 100-101 -0.6447703481009626 2202 1 0.04508358821940561 2202 2 0.06521909958861016 2202 3 0.04575289878850697 2202 4 -0.09949626525013144 2202 5 -0.054862831049241834 2202 6 0.03283315606074666 2202 7 -0.011665228475798983 2202 8 0.003741666556408063 2202 9 -0.02125401710011232 2202 10-11 0.010462089923073847 2202 12-13 0.031466446342165 2202 14-15 0.04120187571597711 2202 16-17 0.015807027595805323 2202 18-19 -0.025013893533007092 2202 20-21 -0.033952947841562775 2202 22-23 0.020149918767998543 2202 24-25 -0.047500397939707284 2202 26-27 -0.09999399872354076 2202 28-29 -0.035581200911586564 2202 30-31 0.04469081047380996 2202 32-33 0.06478102456186008 2202 34-35 -0.004556297066358184 2202 36-37 -0.03237863717419742 2202 38-39 -0.07954034380040298 2202 40-41 -0.09055019970906386 2202 42-43 -0.08548232349445328 2202 44-45 -0.08444279843506308 2202 46-47 -0.09021619227698352 2202 48-49 -0.08610587522403534 2202 50-51 -0.06369306776607431 2202 52-53 -0.06422920746614835 2202 54-55 -0.09329576051633381 2202 56-57 -0.1254125398356365 2202 58-59 -0.11783300821529963 2202 60-61 -0.04672551344339837 2202 62-63 -0.20025717923486042 2202 64-65 -0.14662579296619072 2202 66-67 -0.16255920038913985 2202 68-69 -0.12952309432127862 2202 70-71 -0.1851709454665098 2202 72-73 -0.273113868664403 2202 74-75 -0.2881559877501658 2202 76-77 -0.42342599967179595 2202 78-79 -0.10317998448550725 2202 80-81 -0.03609844134084028 2202 82-83 0.001640242536915082 2202 84-85 -0.02838356743617254 2202 86-87 -0.14274705005950494 2202 88-89 -0.24455158454430403 2202 90-91 -0.11681363492524355 2202 92-93 -0.1494168956592361 2202 94-95 -0.19932573361712969 2202 96-97 -0.23536062217360865 2202 98-99 -0.3046131868704194 2202 100-101 -0.3244964259991434 1102 1 0.06224647751973578 1102 2 0.06514244180603868 1102 3 0.048311690049224865 1102 4 -0.14292173861446145 1102 5 -0.08152031986092823 1102 6 -0.016505147235342577 1102 7 -0.012657193861144833 1102 8 -0.0023522403372524536 1102 9 0.01326838977799838 1102 10-11 0.021916822319575147 1102 12-13 0.02047707052554415 1102 14-15 -0.012949890503101358 1102 16-17 -0.03577150605438817 1102 18-19 0.009847368326077799 1102 20-21 -0.030520334102476454 1102 22-23 -0.02474959394548648 1102 24-25 -0.0037901572268665973 1102 26-27 -0.024383087660424962 1102 28-29 -0.018217484366481074 1102 30-31 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-0.2288187128955883 1213 50-51 -0.13610134261723061 1213 52-53 -0.17629740051510367 1213 54-55 -0.2228204064422954 1213 56-57 -0.17941804563348285 1213 58-59 -0.141439799158249 1213 60-61 -0.20531546390521527 1213 62-63 -0.12922093663325995 1213 64-65 -0.06181805703796783 1213 66-67 -0.019928712188431064 1213 68-69 -0.022795612957615674 1213 70-71 0.01681460898493725 1213 72-73 0.17847139737171602 1213 74-75 0.1285021313225272 1213 76-77 0.38401532339019795 1213 78-79 -0.1766675221508507 1213 80-81 -0.12851904308457307 1213 82-83 -0.0022799622886822135 1213 84-85 -0.015511493251771924 1213 86-87 0.04421615099952447 1213 88-89 -0.008846073021281597 1213 90-91 0.02848004548859251 1213 92-93 0.047333773777999966 1213 94-95 0.14564352831705918 1213 96-97 0.1903918930849926 1213 98-99 0.2727000588139319 1213 100-101 0.8736869549999327 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 48.0 8 499.0 9 1465.0 10 1083.0 11 453.0 12 661.0 13 941.0 14 2358.0 15 4910.0 16 8527.0 17 12534.0 18 17118.0 19 21226.0 20 26375.0 21 33182.0 22 41654.0 23 51376.0 24 63882.0 25 80115.0 26 100875.0 27 127999.0 28 163398.0 29 208590.0 30 267763.0 31 351718.0 32 476567.0 33 675125.0 34 1061723.0 35 2004760.0 36 4333172.0 37 6201121.0 38 1234308.0 39 55.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 20.259819696206574 23.912587532487002 30.161985868573705 25.66560690273272 2 25.521856088642974 23.873535233511173 30.254393268206236 20.350215409639613 3 21.531547488353585 21.903870841724 35.29163726745224 21.272944402470177 4 21.49995962844897 22.459886958512637 34.24548432327752 21.794669089760866 5 27.33817137104357 21.634007816412122 30.467404694342143 20.56041611820217 6 27.522199405846166 22.07308286729379 30.391560837555147 20.013156889304895 7 27.428945031297115 25.847660359260914 26.293540305320175 20.429854304121804 8 25.399993843731956 23.335288507926023 30.12539612483979 21.139321523502236 9 22.656562800790166 22.34642799615808 33.351735757499476 21.645273445552277 10-11 26.261070546985277 23.283046751994714 28.627611197064272 21.828271503955744 12-13 25.581182712402768 26.29334401026697 25.654190247913828 22.471283029416437 14-15 25.431659934125655 24.419534191269786 26.90482301497481 23.24398285962975 16-17 24.964442846899342 24.361142613948246 26.658145372463576 24.016269166688833 18-19 24.72368057435136 24.784580403392592 27.05709107914087 23.434647943115174 20-21 23.843355381891367 25.16797580513424 27.417968820900285 23.570699992074104 22-23 23.88744470565423 25.225319516057766 27.066819091090856 23.820416687197156 24-25 24.290531358191647 25.234574011001516 26.715696998500704 23.759197632306137 26-27 24.04786349548753 25.224306438390276 26.98242910021871 23.74540096590349 28-29 24.09525859725628 25.560510905362012 26.509098130918623 23.83513236646309 30-31 24.141099318448713 25.325004709800876 26.60022419278053 23.933671778969885 32-33 24.169350136146644 25.6356907362957 26.305060032799915 23.88989909475774 34-35 23.942899702367914 25.737684910548214 26.44762648225729 23.87178890482658 36-37 24.068131896582752 25.65531557078373 26.483236242326974 23.793316290306542 38-39 24.193213329600674 25.50117398667129 26.396337934370163 23.909274749357877 40-41 23.962765399751675 25.869208184460284 26.21268990871567 23.95533650707237 42-43 23.99087091952389 25.763471222003776 26.216622020008785 24.029035838463553 44-45 23.98344380639841 25.56221369938842 26.398516055026167 24.05582643918701 46-47 23.86947730418625 25.6968236126694 26.432682276817616 24.00101680632673 48-49 23.90113838371086 25.461336748618198 26.343471663580782 24.294053204090158 50-51 23.842716915782837 25.642715959766743 26.63417862227383 23.880388502176586 52-53 24.08334716646369 25.75386482107574 26.41488738086744 23.747900631593133 54-55 23.74882065143689 25.74521541154691 26.288052930876443 24.217911006139754 56-57 24.012579417411015 25.93812937209793 26.299840123694135 23.74945108679692 58-59 23.868630703802424 25.98118426986155 26.34731540921592 23.802869617120102 60-61 24.13945585915731 25.985925427711948 25.994472923133603 23.880145789997144 62-63 23.862323310914494 26.088523066581743 26.257790314996583 23.791363307507186 64-65 24.027550173553664 26.031496115419156 26.096385430409647 23.844568280617533 66-67 24.29844763947501 25.977026238881223 26.09281530160531 23.631710820038453 68-69 24.14306579936838 25.75310456597972 26.383802969711674 23.720026664940228 70-71 24.08167938136227 26.166028527231706 26.130664449110075 23.62162764229595 72-73 23.93173377542965 25.86561107975807 26.349044833084236 23.853610311728044 74-75 24.02447007749626 25.916427764796207 26.31084243375284 23.748259723954693 76-77 23.945074468110537 25.912713450453495 26.177011866108685 23.965200215327283 78-79 24.096365521850068 25.864015776005576 25.986900902995696 24.05271779914866 80-81 24.073928187543327 25.76940058436233 26.24810439578182 23.908566832312523 82-83 23.864624162577144 26.230403563338434 26.192515381244817 23.712456892839604 84-85 23.903056955081695 26.16115871716156 26.10944246661021 23.82634186114653 86-87 23.77527002635467 26.10744982362873 26.256573442421825 23.860706707594773 88-89 23.6909643108876 26.88129249000273 25.82595400558134 23.60178919352833 90-91 23.816120744075672 26.2416453589299 25.920038599527455 24.022195297466972 92-93 23.72561319410024 26.567477801125193 25.835207036118913 23.87170196865565 94-95 23.676153205493705 26.766986258622598 25.484159828516304 24.072700707367385 96-97 23.68404392509183 26.44363288840903 25.63314226885987 24.23918091763927 98-99 23.95891913018607 26.348512179851173 25.489573939954536 24.202994750008223 100-101 24.116360457439324 26.829429182328063 25.046540530947798 24.007669829284815 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 17154.0 1 10198.0 2 4270.5 3 4862.0 4 4704.5 5 3901.5 6 2588.5 7 2397.5 8 2471.0 9 2543.0 10 2565.0 11 2583.5 12 2649.5 13 2690.5 14 2880.5 15 3101.0 16 3365.5 17 3753.0 18 4096.0 19 4492.0 20 5180.5 21 5970.0 22 6824.5 23 7892.5 24 9107.0 25 10694.5 26 12699.0 27 15189.5 28 18221.0 29 21831.0 30 26063.5 31 32036.5 32 48457.5 33 78682.5 34 116345.5 35 148790.5 36 183864.0 37 248155.5 38 318749.0 39 365248.5 40 413536.0 41 462474.0 42 505805.5 43 569150.0 44 715936.5 45 894236.5 46 1046985.5 47 1187187.5 48 1262547.5 49 1235221.5 50 1102435.5 51 991687.5 52 894991.0 53 802426.0 54 750241.5 55 685734.0 56 601791.0 57 457797.5 58 310583.5 59 219580.0 60 162252.0 61 132554.5 62 115769.0 63 88289.5 64 56599.0 65 37235.5 66 24826.0 67 16163.5 68 12115.0 69 9552.5 70 7783.5 71 6772.0 72 6098.0 73 5457.0 74 4752.0 75 3785.0 76 2905.0 77 2537.0 78 2367.5 79 2064.5 80 1520.0 81 981.0 82 688.5 83 553.0 84 440.5 85 362.5 86 304.5 87 270.5 88 261.0 89 266.5 90 231.5 91 174.0 92 144.0 93 119.5 94 100.0 95 88.0 96 61.5 97 37.0 98 30.5 99 18.5 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11436889461931363 2 0.0 3 0.0 4 0.5712811916395605 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.770756907466455E-4 12-13 0.0 14-15 0.0 16-17 3.1293414278206304E-5 18-19 0.0 20-21 0.0016158781190928348 22-23 5.519020336338203E-4 24-25 0.0 26-27 9.956995452156552E-5 28-29 4.4095265573836155E-4 30-31 0.0030155471940816986 32-33 0.0013228579672150847 34-35 0.001098114355580694 36-37 0.008167581126611846 38-39 0.015751966805311667 40-41 0.01311194058256844 42-43 0.010540190900068578 44-45 0.009194574086105708 46-47 0.016585509567449343 48-49 0.016030762677972047 50-51 0.01511756395221712 52-53 0.01727396468156988 54-55 0.019242604925253405 56-57 0.025598012879572755 58-59 0.033694472610097774 60-61 0.018116042011237975 62-63 0.009095004131584141 64-65 0.01907475843048848 66-67 0.02507455940437367 68-69 0.03240575291300436 70-71 0.031259276008084626 72-73 0.033896457374984375 74-75 0.026943629693535632 76-77 0.03441706599433999 78-79 0.01749017372567385 80-81 0.005925834721954885 82-83 0.008543102097950322 84-85 0.015760501372842083 86-87 0.02993357318502607 88-89 0.025819911635363676 90-91 0.03673277865092726 92-93 0.027083027629865822 94-95 0.037355802080647905 96-97 0.01646318076617999 98-99 0.019145879826575313 100-101 0.29059064498742376 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7575583E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.65550056430726 #Duplication Level Percentage of deduplicated Percentage of total 1 81.46395581197451 20.89998564304814 2 9.09839454640778 4.668477328393098 3 2.691532078351334 2.0715780826498125 4 1.6063951106084309 1.6485148266686007 5 0.9283694308855993 1.1908891228985552 6 0.6101374936471751 0.939202968754207 7 0.35724215852679825 0.6415658479775032 8 0.2847478831139151 0.5844279580731476 9 0.24324362888246284 0.5616483352252354 >10 1.8913245750224166 9.932560739981879 >50 0.3088157988815742 5.661135230128869 >100 0.39470200418063994 21.77093840973659 >500 0.0827032747323932 14.983298659395029 >1k 0.03822771948623579 14.078616177957556 >5k 1.863198534032056E-4 0.2995235825842202 >10k+ 2.2165445539104944E-5 0.06763708652782663 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.827654024335921E-5 0.0 0.0 2 7.965596361725241E-5 0.0 0.0 3 8.534567530419901E-5 0.0 0.0 4 9.672509867809222E-5 0.0 0.0 5 1.0241481036503881E-4 0.0 0.0 6 1.0810452205198542E-4 0.0 0.0 7 1.2517365711282522E-4 0.0 0.0 8 1.3655308048671842E-4 0.0 0.0 9 1.7638106229534461E-4 0.0 0.0 10-11 2.3327817916481063E-4 0.0 0.0 12-13 3.3853784537332275E-4 0.0 0.0 14-15 4.6940121417309456E-4 0.0 0.0 16-17 6.656962673727523E-4 0.0 0.0 18-19 9.217332932853494E-4 0.0 0.0 20-21 0.0012261328685369923 0.0 0.0 22-23 0.0015305324437886356 0.0 0.0 24-25 0.0018605357216315386 0.0 0.0 26-27 0.002258815539717801 0.0 0.0 28-29 0.0028533904110037206 0.0 0.0 30-31 0.0034821035524113197 0.0 0.0 32-33 0.004210386648340485 0.0 0.0 34-35 0.004987032293608695 0.0 0.0 36-37 0.005914455298580992 0.0 0.0 38-39 0.006901620276266228 0.0 0.0 40-41 0.008193184829203105 0.0 0.0 42-43 0.00972371727299174 0.0 5.689711686946601E-6 44-45 0.011493217607632134 5.689711686946601E-6 5.689711686946601E-6 46-47 0.013797550840845507 5.689711686946601E-6 5.689711686946601E-6 48-49 0.01662533754925797 5.689711686946601E-6 5.689711686946601E-6 50-51 0.02001356085883467 5.689711686946601E-6 5.689711686946601E-6 52-53 0.02405610101241023 5.689711686946601E-6 5.689711686946601E-6 54-55 0.029216669512470796 5.689711686946601E-6 5.689711686946601E-6 56-57 0.03561759516028572 5.689711686946601E-6 5.689711686946601E-6 58-59 0.04363439892719348 5.689711686946601E-6 5.689711686946601E-6 60-61 0.05398398448574934 5.689711686946601E-6 5.689711686946601E-6 62-63 0.06702480367223096 5.689711686946601E-6 5.689711686946601E-6 64-65 0.08285927129700335 5.689711686946601E-6 5.689711686946601E-6 66-67 0.10277041734547297 5.689711686946601E-6 5.689711686946601E-6 68-69 0.1272532467344042 5.689711686946601E-6 5.689711686946601E-6 70-71 0.15771311825047285 5.689711686946601E-6 5.689711686946601E-6 72-73 0.19521400797913788 5.689711686946601E-6 1.1379423373893202E-5 74-75 0.24210292199126482 5.689711686946601E-6 1.1379423373893202E-5 76-77 0.2995149577683995 5.689711686946601E-6 1.1379423373893202E-5 78-79 0.36981134566062474 5.689711686946601E-6 1.1379423373893202E-5 80-81 0.45560650818809256 5.689711686946601E-6 1.1379423373893202E-5 82-83 0.5571138095390633 5.689711686946601E-6 1.1379423373893202E-5 84-85 0.6778921643737224 5.689711686946601E-6 1.1379423373893202E-5 86-87 0.822075148232636 5.689711686946601E-6 1.1379423373893202E-5 88-89 0.9921548548346875 5.689711686946601E-6 1.1379423373893202E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 126095 0.0 65.50349 1 TTTGTGT 137440 0.0 60.17121 2 ACTTTGT 37055 0.0 57.97356 1 TTGTGTT 153975 0.0 55.399525 3 TGTGTTT 161245 0.0 53.16558 4 GTGTTTG 177600 0.0 48.3663 5 TTTGAGG 57270 0.0 35.675686 8 GTTTGAG 134475 0.0 35.096146 7 TTGAGGG 31060 0.0 35.091152 9 TGTTTGA 249930 0.0 34.353848 6 TAACTTT 21690 0.0 30.21493 1 TTGAGGC 9415 0.0 29.395136 9 AACTTTG 22500 0.0 29.241228 2 TTGAGTC 15725 0.0 28.176609 4 TGAGTCT 3560 0.0 27.202412 5 GTTTGAC 54655 0.0 26.95995 7 GTTTGAA 20605 0.0 25.905865 2 TTTGAGT 51870 0.0 25.874594 3 TTGAGGT 18020 0.0 25.869257 9 TTGAGCC 18010 0.0 24.24942 9 >>END_MODULE