FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l03n01_8033.34100000035d6e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l03n01_8033.34100000035d6e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17575583
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1260950.065.503491
TTTGTGT1374400.060.171212
ACTTTGT370550.057.973561
TTGTGTT1539750.055.3995253
TGTGTTT1612450.053.165584
GTGTTTG1776000.048.36635
TTTGAGG572700.035.6756868
GTTTGAG1344750.035.0961467
TTGAGGG310600.035.0911529
TGTTTGA2499300.034.3538486
TAACTTT216900.030.214931
TTGAGGC94150.029.3951369
AACTTTG225000.029.2412282
TTGAGTC157250.028.1766094
TGAGTCT35600.027.2024125
GTTTGAC546550.026.959957
GTTTGAA206050.025.9058652
TTTGAGT518700.025.8745943
TTGAGGT180200.025.8692579
TTGAGCC180100.024.249429