FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l03n01_7965.34100000035ca3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l03n01_7965.34100000035ca3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14622067
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT330800.058.7536471
CTTTGTG1015750.055.5822681
TTTGTGT1046100.054.0210382
TAACTTT165850.049.3591961
TTGTGTT1180050.049.30883
AACTTTG177050.046.3987272
GTGTTTG1353700.043.2721825
TGTGTTT1367750.042.8425944
TTTGAGG413250.030.5436928
TTGAGGG247850.030.4908989
GTTTGAG1069750.029.722067
TTGAGGC52100.029.1560259
TTTGACG137700.027.9577988
TGTTTGA2124550.027.6566496
TTTGAAT83600.024.894383
GTTTGAT503500.024.7909582
TTTGATG161350.024.742478
TTGATTC60650.024.4768284
AGTCCCT8950.024.397817
TTTGATT193900.024.3220543
Produced by FastQC (version 0.11.2)