##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C8364ACXX_l03n01_7765.34100000035c6f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14427885 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.73463712803367 33.0 33.0 33.0 33.0 33.0 2 32.74526224737721 33.0 33.0 33.0 33.0 33.0 3 32.77489049850342 33.0 33.0 33.0 33.0 33.0 4 36.46125131992665 37.0 37.0 37.0 37.0 37.0 5 36.57073923170305 37.0 37.0 37.0 37.0 37.0 6 36.608220955462286 37.0 37.0 37.0 37.0 37.0 7 36.59160479862433 37.0 37.0 37.0 37.0 37.0 8 36.602280860985516 37.0 37.0 37.0 37.0 37.0 9 36.59247159233664 37.0 37.0 37.0 37.0 37.0 10-11 36.57756521485997 37.0 37.0 37.0 37.0 37.0 12-13 36.538339888348155 37.0 37.0 37.0 37.0 37.0 14-15 38.59530655394051 40.0 37.0 40.0 37.0 40.0 16-17 38.55738900746714 40.0 37.0 40.0 37.0 40.0 18-19 38.51808040471629 40.0 37.0 40.0 37.0 40.0 20-21 38.470456272696936 40.0 37.0 40.0 37.0 40.0 22-23 38.416456154176444 40.0 37.0 40.0 37.0 40.0 24-25 38.36852688387799 40.0 37.0 40.0 37.0 40.0 26-27 38.27866031646357 40.0 37.0 40.0 37.0 40.0 28-29 38.21238372776051 40.0 37.0 40.0 37.0 40.0 30-31 38.15408997923119 40.0 37.0 40.0 37.0 40.0 32-33 38.05413984794029 40.0 37.0 40.0 37.0 40.0 34-35 37.988369189247074 40.0 37.0 40.0 37.0 40.0 36-37 37.88413849292533 40.0 37.0 40.0 33.0 40.0 38-39 37.763073728408564 40.0 37.0 40.0 33.0 40.0 40-41 37.62796698892457 40.0 37.0 40.0 33.0 40.0 42-43 37.49011161372579 40.0 37.0 40.0 33.0 40.0 44-45 37.32603780803631 40.0 37.0 40.0 33.0 40.0 46-47 37.13672107866122 37.0 37.0 40.0 33.0 40.0 48-49 36.910912826100294 37.0 37.0 40.0 33.0 40.0 50-51 36.69865967880948 37.0 37.0 40.0 33.0 40.0 52-53 36.46495706058096 37.0 37.0 40.0 33.0 40.0 54-55 36.218572611300964 37.0 37.0 40.0 33.0 40.0 56-57 35.94989740353489 37.0 37.0 40.0 33.0 40.0 58-59 35.67000225604792 37.0 33.0 38.5 33.0 40.0 60-61 35.37371724961767 37.0 33.0 37.0 33.0 40.0 62-63 35.113154526806944 37.0 33.0 37.0 30.0 40.0 64-65 34.817622749280304 37.0 33.0 37.0 27.0 40.0 66-67 34.57529336420411 37.0 33.0 37.0 27.0 37.0 68-69 34.29988657381175 37.0 33.0 37.0 27.0 37.0 70-71 34.0473188897749 37.0 33.0 37.0 27.0 37.0 72-73 33.82280514434375 37.0 33.0 37.0 27.0 37.0 74-75 33.57061007209303 37.0 33.0 37.0 27.0 37.0 76-77 30.671416150045555 33.0 30.0 33.0 24.5 35.0 78-79 33.33514208770031 35.0 33.0 37.0 27.0 37.0 80-81 33.84149059269602 37.0 33.0 37.0 27.0 37.0 82-83 33.8247737974069 37.0 33.0 37.0 27.0 37.0 84-85 33.73492805771602 37.0 33.0 37.0 27.0 37.0 86-87 33.64823357685482 37.0 33.0 37.0 27.0 37.0 88-89 33.49987884572133 37.0 33.0 37.0 27.0 37.0 90-91 33.39296175426959 37.0 33.0 37.0 27.0 37.0 92-93 33.26828984289797 37.0 33.0 37.0 27.0 37.0 94-95 33.1501635548107 35.0 33.0 37.0 27.0 37.0 96-97 33.052537845983665 33.0 33.0 37.0 27.0 37.0 98-99 32.89599338364563 33.0 33.0 37.0 27.0 37.0 100-101 30.935445250637912 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.0946186720032216 2306 2 0.10313145082137964 2306 3 0.09375188439832272 2306 4 -0.09186355724384754 2306 5 -0.04222652596308052 2306 6 0.07973222835638438 2306 7 0.09034054123327451 2306 8 0.0347229779625593 2306 9 0.08849196169335727 2306 10-11 0.11484382502943902 2306 12-13 0.11993919007942822 2306 14-15 0.20128719191372113 2306 16-17 0.16840605046635204 2306 18-19 0.14823380142177456 2306 20-21 0.12740264524247635 2306 22-23 0.1170866459580111 2306 24-25 0.085614642926366 2306 26-27 0.12521274811563643 2306 28-29 0.1078857330196783 2306 30-31 0.1258438160241795 2306 32-33 0.1486748010211869 2306 34-35 0.10391315152131142 2306 36-37 0.11654020414360389 2306 38-39 0.003116999130092779 2306 40-41 -0.07526455177683289 2306 42-43 -0.0025866473407774038 2306 44-45 -0.05219963719964227 2306 46-47 -0.057790896662545777 2306 48-49 -0.03394790513142709 2306 50-51 -0.034670453803272494 2306 52-53 -0.05291321460244802 2306 54-55 -0.006602276274122687 2306 56-57 -0.1917892187518575 2306 58-59 -0.2784617801289784 2306 60-61 -0.15145019284411632 2306 62-63 -0.13942217859590045 2306 64-65 -0.21286487536745824 2306 66-67 -0.23066412348882182 2306 68-69 -0.27716886737994173 2306 70-71 -0.3307461691617277 2306 72-73 -0.380635904935275 2306 74-75 -0.43008538852342326 2306 76-77 -0.7582142058386268 2306 78-79 -0.2798475719494249 2306 80-81 -0.11871451344812556 2306 82-83 -0.12891527752610443 2306 84-85 -0.3053495698008035 2306 86-87 -0.39350880663261023 2306 88-89 -0.4260983323559131 2306 90-91 -0.4431661688036357 2306 92-93 -0.4651059109630964 2306 94-95 -0.4774116584379726 2306 96-97 -0.5517499874685967 2306 98-99 -0.606028578550962 2306 100-101 -0.8679023579592595 2307 1 0.08981675504303865 2307 2 0.08122926672572106 2307 3 0.07440640604407633 2307 4 -0.10190878132856085 2307 5 -0.044245432492594716 2307 6 0.06695490403804882 2307 7 0.08767931719943789 2307 8 0.12413876976765437 2307 9 0.0676921139823321 2307 10-11 0.0735646041663145 2307 12-13 0.09430689328486608 2307 14-15 0.13568815126460265 2307 16-17 0.08595393858961842 2307 18-19 0.08439314128850128 2307 20-21 0.11258170180126825 2307 22-23 0.0904328253304314 2307 24-25 0.05856846080006761 2307 26-27 0.009549379307038919 2307 28-29 -0.0037786118216800446 2307 30-31 0.007081137359747913 2307 32-33 -0.015829383496893 2307 34-35 0.06855309630567064 2307 36-37 0.045022724802315395 2307 38-39 0.0032533013085185303 2307 40-41 -0.013646435073773944 2307 42-43 -0.10723436891431248 2307 44-45 -0.05253191409196489 2307 46-47 -0.043570651561829266 2307 48-49 -0.0970486608119785 2307 50-51 -0.2256145163875516 2307 52-53 -0.21101212195051033 2307 54-55 -0.14271865735775435 2307 56-57 -0.16254779483811888 2307 58-59 -0.2817760821608246 2307 60-61 -0.3625900382571814 2307 62-63 -0.3229480858917242 2307 64-65 -0.3695096815935912 2307 66-67 -0.3608532335675321 2307 68-69 -0.46388070883831745 2307 70-71 -0.5580666404707273 2307 72-73 -0.5585754754954806 2307 74-75 -0.5837970661019583 2307 76-77 -0.7462770902570703 2307 78-79 -0.38008345223408213 2307 80-81 -0.2206916227680722 2307 82-83 -0.3104089715113787 2307 84-85 -0.33554144697468047 2307 86-87 -0.4013884619242347 2307 88-89 -0.4580425722550885 2307 90-91 -0.4895059977587479 2307 92-93 -0.48782726485338657 2307 94-95 -0.6010444673511799 2307 96-97 -0.635296027150531 2307 98-99 -0.7470467066085149 2307 100-101 -1.103339413681688 2201 1 0.06689256662464516 2201 2 0.07986306714617086 2201 3 0.08439559472531499 2201 4 -0.004914368068227759 2201 5 0.005705148406448757 2201 6 0.049001366541048696 2201 7 0.09106919363685506 2201 8 0.08885870125254769 2201 9 0.08342811579011311 2201 10-11 0.07906914826965306 2201 12-13 0.07964225408937153 2201 14-15 0.12380082512842705 2201 16-17 0.04509362871322509 2201 18-19 0.059396371542149495 2201 20-21 0.010010100074616446 2201 22-23 0.07239034682630319 2201 24-25 0.06659425276173181 2201 26-27 0.12071780246410668 2201 28-29 0.06298456703168398 2201 30-31 0.035248091405598814 2201 32-33 0.048291340298561636 2201 34-35 -0.007180738237416051 2201 36-37 -0.0168234831997367 2201 38-39 0.015915430384687568 2201 40-41 3.1177149173089447E-4 2201 42-43 -0.024216952061216546 2201 44-45 -0.08812632175823154 2201 46-47 -0.08793788005421987 2201 48-49 -0.06508971317802548 2201 50-51 -0.15402502312625188 2201 52-53 -0.1548126502699816 2201 54-55 -0.09013537612715794 2201 56-57 -0.18889310098591494 2201 58-59 -0.16443695867779695 2201 60-61 -0.1720148062604281 2201 62-63 -0.21598259779371176 2201 64-65 -0.21677154299192836 2201 66-67 -0.36709803201599556 2201 68-69 -0.4989614596550922 2201 70-71 -0.5103919273614537 2201 72-73 -0.43300438787635187 2201 74-75 -0.5476540604762619 2201 76-77 -0.9768764878074983 2201 78-79 -0.47872356487834367 2201 80-81 -0.26868490546870305 2201 82-83 -0.20240107969062393 2201 84-85 -0.3272723392829633 2201 86-87 -0.38687198989852334 2201 88-89 -0.3670511463542496 2201 90-91 -0.3092152057319524 2201 92-93 -0.39612040755116595 2201 94-95 -0.48443551639102367 2201 96-97 -0.48877343008707896 2201 98-99 -0.6288595470499132 2201 100-101 -0.8561400595812216 1101 1 0.04066974929553169 1101 2 0.06751128878492096 1101 3 0.04044349009279813 1101 4 -0.10062838989792056 1101 5 -0.08402252219011785 1101 6 0.013168490687775147 1101 7 0.014423035894189695 1101 8 0.04833549960763861 1101 9 0.05683787433613219 1101 10-11 0.03209600686127345 1101 12-13 0.018808355484672745 1101 14-15 0.05391653615823344 1101 16-17 0.03769370344732437 1101 18-19 0.054465699167451476 1101 20-21 0.03771015567757274 1101 22-23 0.006038473731685201 1101 24-25 0.026944794841689657 1101 26-27 0.0513650356420996 1101 28-29 -0.003450530085757464 1101 30-31 0.01910360082912632 1101 32-33 -0.06693363164494315 1101 34-35 0.02321600358582998 1101 36-37 -0.10126471245795443 1101 38-39 -0.06602763019539992 1101 40-41 0.009234935107649278 1101 42-43 -0.11343629892618878 1101 44-45 -0.14488599039436423 1101 46-47 -0.10642900864873894 1101 48-49 -0.08045358602240071 1101 50-51 -0.09896984975659251 1101 52-53 -0.03735843630082769 1101 54-55 -0.1740029795183773 1101 56-57 -0.18659654252002866 1101 58-59 -0.2417633003703088 1101 60-61 -0.25251806276062183 1101 62-63 -0.35461521631513904 1101 64-65 -0.3727945233752834 1101 66-67 -0.4594255869032864 1101 68-69 -0.3605731518957924 1101 70-71 -0.2551745760011386 1101 72-73 -0.3960439591053131 1101 74-75 -0.4473998226501479 1101 76-77 -0.7070282569287158 1101 78-79 -0.30596255220577717 1101 80-81 -0.1943071262705871 1101 82-83 -0.15148427528258424 1101 84-85 -0.18043180492058042 1101 86-87 -0.2842622497565941 1101 88-89 -0.24255568224322843 1101 90-91 -0.2476502840220789 1101 92-93 -0.3203943530646143 1101 94-95 -0.47958096371482384 1101 96-97 -0.4545327672649435 1101 98-99 -0.5628051060733625 1101 100-101 -0.7829119431789806 2304 1 0.07966355905011113 2304 2 0.08036949868161258 2304 3 0.05803350259395046 2304 4 -0.1255134070649575 2304 5 -0.08769203662196645 2304 6 -0.014349385643008361 2304 7 0.04779325788133093 2304 8 0.033960806673739796 2304 9 0.03469073547903889 2304 10-11 0.010049742322351563 2304 12-13 0.016971230697514272 2304 14-15 0.046691968314796384 2304 16-17 0.04876548930554492 2304 18-19 0.04331237099934526 2304 20-21 0.04044656609335817 2304 22-23 0.0359546018579735 2304 24-25 0.026658448150186587 2304 26-27 0.06974861066783689 2304 28-29 -0.04878255846433888 2304 30-31 -0.05082596790433058 2304 32-33 0.031355346093732805 2304 34-35 -0.0068896312551629535 2304 36-37 -0.044840249843275615 2304 38-39 -0.07687247645940687 2304 40-41 -0.08065221419933266 2304 42-43 -0.1301399180132563 2304 44-45 -0.07867788376228901 2304 46-47 -0.12801623900747217 2304 48-49 -0.0584024790111215 2304 50-51 -0.07446236549775165 2304 52-53 -0.01486434724230179 2304 54-55 -0.15620467757076284 2304 56-57 -0.15067205842041886 2304 58-59 -0.17716006925809324 2304 60-61 -0.1954710362425729 2304 62-63 -0.2425148508164412 2304 64-65 -0.22369193325614845 2304 66-67 -0.2516339680726105 2304 68-69 -0.40298881550459953 2304 70-71 -0.3393437124865457 2304 72-73 -0.26851053635481037 2304 74-75 -0.3762834599082865 2304 76-77 -0.6792916107028368 2304 78-79 -0.32192434809151393 2304 80-81 -0.12143325542686512 2304 82-83 -0.15895317963362743 2304 84-85 -0.18214022537895147 2304 86-87 -0.27488768416645826 2304 88-89 -0.3150087883281074 2304 90-91 -0.33334103012560234 2304 92-93 -0.29970005377332853 2304 94-95 -0.3761049783433634 2304 96-97 -0.34434273816932404 2304 98-99 -0.524242001720026 2304 100-101 -0.6703120233076945 2202 1 0.05402843613944697 2202 2 0.042558107158981784 2202 3 0.030860689543231956 2202 4 -0.09433111748570866 2202 5 -0.050744693809051 2202 6 0.04095697119811348 2202 7 0.028556009636815816 2202 8 0.039713387456210114 2202 9 -0.030866110656461387 2202 10-11 0.020757828603173323 2202 12-13 0.03262371108321105 2202 14-15 0.06190400573440513 2202 16-17 -0.008188826816116546 2202 18-19 2.8018505877014377E-4 2202 20-21 -0.04375105061482287 2202 22-23 0.018772644611473766 2202 24-25 0.016779586275085023 2202 26-27 -0.0954054808933833 2202 28-29 0.010711368132461985 2202 30-31 0.08146972084917792 2202 32-33 0.0591360122080431 2202 34-35 0.0056394035669598 2202 36-37 -0.006107645187384492 2202 38-39 -0.05367592308929403 2202 40-41 -0.03515257730840915 2202 42-43 -0.047810728467027275 2202 44-45 -0.0683702300443727 2202 46-47 -0.06868722332463761 2202 48-49 -0.02731762239022828 2202 50-51 -0.031189524608706165 2202 52-53 -0.03735980336288236 2202 54-55 -3.379040469937422E-4 2202 56-57 -0.0679043266955972 2202 58-59 -0.025699860847780087 2202 60-61 -0.005037914358936746 2202 62-63 -0.16183130909930554 2202 64-65 -0.20217567015365034 2202 66-67 -0.14310068656272534 2202 68-69 -0.17161897921379676 2202 70-71 -0.17595290051307444 2202 72-73 -0.21843200801863816 2202 74-75 -0.2695868495028151 2202 76-77 -0.4308536708157362 2202 78-79 -0.026580552307613914 2202 80-81 0.005845023169300134 2202 82-83 -0.02609546791470052 2202 84-85 0.0045396684219340955 2202 86-87 -0.08689266835976639 2202 88-89 -0.17536264878172148 2202 90-91 -0.10983330845805739 2202 92-93 -0.05118596505437978 2202 94-95 -0.11644221371179242 2202 96-97 -0.19468150191169542 2202 98-99 -0.3778372954828626 2202 100-101 -0.3517598646314539 1102 1 0.05159576017976519 1102 2 0.04723201682585909 1102 3 0.04252638048180302 1102 4 -0.09142336498507575 1102 5 -0.07708743530480433 1102 6 -0.03929571689030098 1102 7 -0.013643827406347953 1102 8 0.019913524777031455 1102 9 0.02943407070827675 1102 10-11 0.020038564070809173 1102 12-13 0.03284621804346699 1102 14-15 -0.038929884519866675 1102 16-17 -0.058695287207889635 1102 18-19 -0.05986816918050408 1102 20-21 -0.013606297136810497 1102 22-23 -0.0237679021299968 1102 24-25 -0.04248253062745988 1102 26-27 0.001998645597751647 1102 28-29 -0.013660474502707132 1102 30-31 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50-51 -0.10262482480366941 1213 52-53 -0.09025919504201596 1213 54-55 -0.12653129598485435 1213 56-57 -0.13879518079465925 1213 58-59 -0.11954059144774476 1213 60-61 -0.1439020553982715 1213 62-63 -0.09833584225766145 1213 64-65 -0.008857747437282626 1213 66-67 0.043817813458474575 1213 68-69 0.08527809394875874 1213 70-71 0.15349215671071192 1213 72-73 0.16974353049131707 1213 74-75 0.17996688565843044 1213 76-77 0.49874510986787257 1213 78-79 -0.07793343299147892 1213 80-81 -0.10949832930592152 1213 82-83 0.07319071279090394 1213 84-85 0.044532687276706895 1213 86-87 0.07690161356836711 1213 88-89 0.037852552170683396 1213 90-91 0.08032202828196233 1213 92-93 0.10108582674721589 1213 94-95 0.18363296467036605 1213 96-97 0.2534990549168583 1213 98-99 0.31061509761931916 1213 100-101 0.9149425490755547 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 42.0 8 448.0 9 1289.0 10 823.0 11 380.0 12 528.0 13 692.0 14 1586.0 15 3058.0 16 5422.0 17 8222.0 18 11705.0 19 15308.0 20 19519.0 21 25395.0 22 32334.0 23 40789.0 24 51954.0 25 65891.0 26 84751.0 27 108023.0 28 138166.0 29 176661.0 30 229799.0 31 304606.0 32 416039.0 33 599279.0 34 949241.0 35 1773605.0 36 3776026.0 37 4852593.0 38 733688.0 39 21.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 20.255769918370696 22.81960293949587 31.095093283940678 25.829533858192754 2 26.727777494761014 22.72477220327165 30.167623321089682 20.379826980877656 3 21.50444781061119 20.918499142459204 37.195146759209685 20.381906287719925 4 21.430694991070233 20.8525831078963 36.2043586297044 21.51236327132906 5 28.069249235074995 20.058179005446743 32.004136434411556 19.868435325066702 6 29.29764133828347 20.59291434607359 30.77166888979223 19.337775425850705 7 28.02005283518686 25.724290150635383 26.47556450581634 19.780092508361413 8 26.950748498480547 21.974322639804793 30.424771198273344 20.650157663441316 9 22.88911368506195 21.19592026135501 34.569439664926634 21.34552638865641 10-11 27.296715068855654 22.03690779829012 28.90536049489489 21.761016637959337 12-13 26.605330580331078 25.77990467764333 25.237579867042186 22.377184874983406 14-15 26.402456077242093 23.661645487193724 26.54462868258237 23.391269752981813 16-17 25.964180048338353 23.41144898547472 26.067597868321755 24.556773097865168 18-19 25.64372740703159 24.033259206044406 26.695274463304912 23.627738923619088 20-21 24.29126588075633 24.71300837957414 27.197180689776584 23.798545049892937 22-23 24.471499131070754 24.714415339897805 26.716652061197486 24.097433467833955 24-25 24.711362753445844 24.597704375935905 26.49227520180539 24.19865766881286 26-27 24.569226939569976 24.542632576486778 26.78129643465378 24.106844049289467 28-29 24.80803304257885 24.758541811807387 26.116915122625866 24.3165100229879 30-31 24.87203734048378 24.567312687459484 26.104285504071374 24.45636446798537 32-33 24.695387573800858 25.01474249702551 25.92444536120088 24.365424567972756 34-35 24.5381622986787 25.117682360570694 25.79057133408067 24.55358400666994 36-37 24.577378128988492 25.166502422215746 25.971130407321386 24.284989041474375 38-39 24.766810733789153 24.93244026621483 25.846563748705208 24.45418525129081 40-41 24.488739362145697 25.52596676588364 25.63931798225685 24.345975889713813 42-43 24.455989501452063 25.37902103757728 25.808675026909043 24.35631443406161 44-45 24.72873663758928 24.96370510715385 26.127207304637228 24.18035095061964 46-47 24.255221036543094 25.29356372553913 26.1155324520044 24.335682785913377 48-49 24.48605467907175 25.009886113429047 26.145892310771785 24.35816689672742 50-51 24.26490655589756 25.22742597352236 26.44643238035939 24.061235090220684 52-53 24.344919431143605 25.49130306114307 26.208945313889153 23.954832193824167 54-55 24.18660019820332 25.245741160720247 26.06736188375716 24.50029675731927 56-57 24.286680771036902 25.49803941442726 26.144414418764605 24.070865395771232 58-59 24.108831188514664 25.466081973074633 26.36253572665926 24.062551111751443 60-61 24.559593933121253 25.36142628079591 25.69424639225356 24.384733393829276 62-63 24.249858888343056 25.604918969018097 26.035958501146396 24.109263641492447 64-65 24.458497659836695 25.670289397312757 25.939665800091 23.931547142759545 66-67 24.786628851104986 25.820334623975 25.47831267554458 23.91472384937543 68-69 24.75065032292722 25.737617933971606 25.710515800945682 23.801215942155494 70-71 24.515211411718596 26.216362787865705 25.496547299798404 23.771878500617298 72-73 24.78113901968765 25.763755323042314 25.49814184270838 23.956963814561654 74-75 24.624020038768613 25.685213712663025 25.571906345240308 24.118859903328055 76-77 24.756623572117725 25.456118575801607 25.50063781910759 24.286620032973083 78-79 24.925966703144333 25.279634358215645 25.43656387341488 24.35783506522514 80-81 24.7101461807245 25.284986199978714 25.57621851378985 24.42864910550694 82-83 24.377677259739265 25.719282536326304 25.612535262991315 24.290504940943112 84-85 24.425829955590636 25.640281858015783 25.548271571552743 24.38561661484084 86-87 24.149646551907328 25.709093113819133 25.743100006645385 24.398160327628162 88-89 24.125076295540566 26.37427299119838 25.23769475294665 24.262955960314393 90-91 24.220607838367382 25.851897990751826 25.414505610227145 24.512988560653643 92-93 23.97211171467787 26.248307722332488 25.323874777258908 24.45570578573074 94-95 24.101184936525406 26.550056066899625 24.945390025066732 24.40336897150824 96-97 24.047752319158754 26.119870003231444 25.129958814761604 24.702418862848198 98-99 24.335226717939513 25.972569452881256 25.26105023071151 24.43115359846772 100-101 24.845930100203912 26.353152490604685 24.522532585994945 24.278384823196454 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 18370.0 1 10676.5 2 4807.0 3 5870.0 4 5665.5 5 4526.0 6 2600.0 7 2433.5 8 2728.5 9 3025.0 10 2916.5 11 2481.5 12 2140.0 13 2052.0 14 2021.5 15 2053.5 16 2123.0 17 2283.5 18 2519.0 19 2630.5 20 2746.0 21 3047.0 22 3423.5 23 3737.0 24 4167.5 25 4718.5 26 5220.0 27 5836.5 28 6595.0 29 7552.5 30 9333.0 31 12624.5 32 27035.5 33 49135.5 34 71324.5 35 109796.0 36 147013.0 37 205314.5 38 277218.5 39 293181.5 40 281023.0 41 291327.5 42 332279.0 43 403295.5 44 492581.5 45 597841.5 46 702229.0 47 794988.0 48 903775.0 49 989996.0 50 975250.0 51 899957.0 52 843453.0 53 820338.0 54 768214.5 55 672303.5 56 586358.0 57 472714.0 58 363923.0 59 292413.0 60 222197.5 61 150101.0 62 95157.0 63 60566.5 64 32274.5 65 16711.5 66 9551.0 67 6231.5 68 4789.0 69 3739.5 70 3001.5 71 2485.5 72 2208.0 73 1987.5 74 1648.0 75 1250.0 76 967.5 77 873.5 78 839.0 79 721.0 80 551.5 81 376.5 82 259.5 83 232.0 84 181.0 85 119.0 86 101.0 87 97.5 88 92.0 89 86.0 90 80.0 91 76.0 92 70.0 93 55.5 94 44.0 95 39.5 96 33.5 97 32.0 98 25.0 99 11.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11107657151412005 2 0.0 3 0.0 4 0.5686626972699047 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.51516143911599E-4 12-13 0.0 14-15 0.0 16-17 2.7724091230280807E-5 18-19 0.0 20-21 0.001680773030835774 22-23 4.886371079336992E-4 24-25 0.0 26-27 1.0743085351733813E-4 28-29 5.302232447791205E-4 30-31 0.0030080638984854675 32-33 0.0014347217211670316 34-35 0.0011817393886907194 36-37 0.008441985779620506 38-39 0.01606264535654394 40-41 0.013349149927380208 42-43 0.010458913416623434 44-45 0.0087920024314028 46-47 0.016613661669745775 48-49 0.016270576040771048 50-51 0.015262112222269583 52-53 0.017480039520692048 54-55 0.01939646732698521 56-57 0.02599480103979204 58-59 0.03349070220617921 60-61 0.018540486010250292 62-63 0.009467777155140897 64-65 0.01882812345676445 66-67 0.025398733078341007 68-69 0.032340152420122566 70-71 0.031439119455138435 72-73 0.033681305333387396 74-75 0.026649782695107427 76-77 0.034020925450958334 78-79 0.018152348733026357 80-81 0.00633842035752295 82-83 0.00892022635334285 84-85 0.01610769700479315 86-87 0.030070242450643322 88-89 0.02573488768450816 90-91 0.03639826627395492 92-93 0.027082971620580563 94-95 0.037306230261746606 96-97 0.01697754036714321 98-99 0.019652915170865308 100-101 0.28916227153182883 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4427885E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.833033956312438 #Duplication Level Percentage of deduplicated Percentage of total 1 76.7315303492031 13.683559862371572 2 9.110296195863105 3.2492844282578157 3 3.5986638894420517 1.9252528601332648 4 1.937521223534396 1.3820752708145962 5 1.2454769561930747 1.1105316425797882 6 0.9110869136749398 0.974846632123026 7 0.8992547312755268 1.1225508110747762 8 0.540626659631467 0.7712810863116576 9 0.33932058110885316 0.5446003900490863 >10 3.0766724862717676 11.359378498784388 >50 0.5995542897953616 7.674077257247158 >100 0.8810229659557334 34.19199228752348 >500 0.09356204805994957 11.123384674284228 >1k 0.03509271984969262 10.463261631095309 >5k 2.791019880706116E-4 0.32503045776933814 >10k+ 3.888815316120925E-5 0.09889220958023481 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.0396534211355302E-4 0.0 0.0 2 1.0396534211355302E-4 0.0 0.0 3 1.0396534211355302E-4 0.0 0.0 4 1.1089636492112323E-4 0.0 0.0 5 1.2475841053626364E-4 0.0 0.0 6 1.3168943334383384E-4 0.0 0.0 7 1.3168943334383384E-4 0.0 0.0 8 1.4555147895897424E-4 0.0 0.0 9 1.5248250176654445E-4 0.0 0.0 10-11 1.9753415001575077E-4 0.0 0.0 12-13 2.911029579179485E-4 0.0 0.0 14-15 4.539819938958482E-4 0.0 0.0 16-17 5.960679614510373E-4 0.0 3.465511403785101E-6 18-19 8.005331342743583E-4 0.0 6.931022807570202E-6 20-21 0.0011158946720188025 0.0 6.931022807570202E-6 22-23 0.0014451182553783869 0.0 6.931022807570202E-6 24-25 0.0017396867247001205 0.0 6.931022807570202E-6 26-27 0.002093168887886201 0.0 6.931022807570202E-6 28-29 0.002609530087050181 0.0 6.931022807570202E-6 30-31 0.0031328223090217313 0.0 6.931022807570202E-6 32-33 0.0038432521467976766 0.0 6.931022807570202E-6 34-35 0.0046888369293212415 0.0 1.0396534211355302E-5 36-37 0.0055309562004410215 0.0 1.3862045615140404E-5 38-39 0.006782005817207442 0.0 1.3862045615140404E-5 40-41 0.008175141401529053 0.0 1.3862045615140404E-5 42-43 0.009890569546402678 0.0 1.3862045615140404E-5 44-45 0.01205997968517215 0.0 1.3862045615140404E-5 46-47 0.014655647726607192 0.0 1.3862045615140404E-5 48-49 0.018013728276874953 0.0 1.3862045615140404E-5 50-51 0.02201985945965053 0.0 1.3862045615140404E-5 52-53 0.027030988949523785 0.0 1.7327557018925503E-5 54-55 0.032950082427188734 0.0 2.0793068422710605E-5 56-57 0.0402380529093488 0.0 2.0793068422710605E-5 58-59 0.04954988205131937 0.0 2.0793068422710605E-5 60-61 0.06092369047854207 0.0 2.0793068422710605E-5 62-63 0.07411342688134817 0.0 2.0793068422710605E-5 64-65 0.09062658872038418 0.0 2.0793068422710605E-5 66-67 0.111367674472038 0.0 2.0793068422710605E-5 68-69 0.1362084602143696 0.0 2.0793068422710605E-5 70-71 0.16654554704310437 3.465511403785101E-6 2.0793068422710605E-5 72-73 0.20326264036620753 6.931022807570202E-6 2.0793068422710605E-5 74-75 0.2473682040021805 6.931022807570202E-6 2.0793068422710605E-5 76-77 0.29982218460987176 6.931022807570202E-6 2.0793068422710605E-5 78-79 0.3642772312088709 6.931022807570202E-6 2.0793068422710605E-5 80-81 0.44076106789040803 6.931022807570202E-6 2.0793068422710605E-5 82-83 0.5324238445205239 6.931022807570202E-6 2.0793068422710605E-5 84-85 0.6409220755502278 6.931022807570202E-6 2.0793068422710605E-5 86-87 0.7690143080569327 6.931022807570202E-6 2.0793068422710605E-5 88-89 0.9183743840486669 6.931022807570202E-6 2.0793068422710605E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGTG 134760 0.0 59.887676 1 TTTGTGT 142615 0.0 56.65582 2 ACTTTGT 46575 0.0 55.32076 1 TTGTGTT 157820 0.0 52.682713 3 TGTGTTT 169375 0.0 49.398693 4 GTGTTTG 190140 0.0 44.130806 5 TAACTTT 26150 0.0 40.68414 1 AACTTTG 26470 0.0 40.401806 2 TTGAGTC 17375 0.0 32.561188 4 TTTGAGG 59505 0.0 32.07037 8 GTTTGAG 146540 0.0 32.009296 7 TGTTTGA 266550 0.0 31.449865 6 TTGAGGC 8210 0.0 30.587496 9 TTTGAGT 52800 0.0 29.197176 3 TTGAGGG 38185 0.0 28.668034 9 GTTTGAC 50930 0.0 28.037258 7 TTGACGC 3000 0.0 27.216896 9 TTTGACC 13165 0.0 26.431055 8 TTTGACG 17330 0.0 26.214693 8 TGAGTGG 7615 0.0 25.870783 5 >>END_MODULE